Newsletter 109
November 21, 2005


The NIH X-Ray Diffraction Interest Group

Newsletter web site: http://mcl1.ncifcrf.gov/nihxray

Item 1: October 2005 Publications by Members:

1:  Tang C, Iwahara J, Clore GM.
 Accurate Determination of Leucine and Valine Side-chain Conformations using U-[(15)N/(13)C/(2)H]/[(1)H-(methine/methyl)-Leu/Val] Isotope Labeling, NOE Pattern Recognition, and Methine Cgamma Hgamma/Cbeta-Hbeta Residual Dipolar Couplings: Application to the 34-kDa Enzyme IIA(Chitobiose).
J Biomol NMR. 2005 Oct;33(2):105-21. PMID: 16258829

2:  Gan J, Tropea JE, Austin BP, Court DL, Waugh DS, Ji X.
 Intermediate states of ribonuclease III in complex with double-stranded RNA.
Structure (Camb). 2005 Oct;13(10):1435-42. PMID: 16216575

3:  Lee JY, Chang J, Joseph N, Ghirlando R, Rao DN, Yang W.
 MutH complexed with hemi- and unmethylated DNAs: coupling base recognition and DNA cleavage.
Mol Cell. 2005 Oct 7;20(1):155-66. PMID: 16209953

4:  Ronning DR, Guynet C, Ton-Hoang B, Perez ZN, Ghirlando R, Chandler M, Dyda F.
 Active site sharing and subterminal hairpin recognition in a new class of DNA transposases.
Mol Cell. 2005 Oct 7;20(1):143-54. PMID: 16209952

5:  Phan J, Austin BP, Waugh DS.
 Crystal structure of the Yersinia type III secretion protein YscE.
Protein Sci. 2005 Oct;14(10):2759-63. PMID: 16195558

6:  Zhou T, Hamer DH, Hendrickson WA, Sattentau QJ, Kwong PD.
 Interfacial metal and antibody recognition.
PNAS USA. 2005 Oct 11;102(41):14575-80. PMID: 16195378

7:  Phan J, Shi ZD, Burke TR Jr, Waugh DS.
 Crystal structures of a high-affinity macrocyclic peptide mimetic in complex with the Grb2 SH2 domain.
J Mol Biol. 2005 Oct 14;353(1):104-15. PMID: 16165154

8:  Ramasamy V, Ramakrishnan B, Boeggeman E, Ratner DM, Seeberger PH, Qasba PK.
 Oligosaccharide preferences of beta1,4-galactosyltransferase-I: crystal
structures of Met340His mutant of human beta1,4-galactosyltransferase-I with a pentasaccharide and trisaccharides of the N-glycan moiety.
J Mol Biol. 2005 Oct 14;353(1):53-67. PMID: 16157350

9:  Bradley CM, Ronning DR, Ghirlando R, Craigie R, Dyda F.
 Structural basis for DNA bridging by barrier-to-autointegration factor.
Nat Struct Mol Biol. 2005 Oct;12(10):935-6. PMID: 16155580

10:  Dauter Z.
 Efficient use of synchrotron radiation for macromolecular diffraction data
collection.
Prog Biophys Mol Biol. 2005 Oct;89(2):153-72. Review. PMID: 15910916

11:  Hall TM.
 Structure and function of argonaute proteins.
Structure (Camb). 2005 Oct;13(10):1403-8. PMID: 16216572


Item 2: Tips and Tricks

This section is always open for contributions. Click for Introduction and tips and tricks in Crystallization, Post-crystallization treatments for improving diffraction quality of protein crystals, Derivatization, Diffraction, Symmetry, Structure Solution, Structure Refinement, and Structure Analysis.

 

Item 3: Topic Discussion

Click for previous discussions on: PHASER, HKL2000, Parallel Protein Expression, Structural Genomics, NCS, Missing Atoms, Trends in Crystallography, and Absorption Correction.

 

Item 4: Lectures

Dr. Zbigniew Dauter's Lectures at the NIH (03/29-31)

Part 1: "How to read international tables?"

Part 2: "Data collection strategy" and "Twinning"

           "Phasing methods - a general introduction to all methods"

Part 3: "SAD phasing, Quick halide soaking, and Radiation damage 

           with possible use of it for phasing"

 

 

This site is maintained by Dr. Xinhua Ji (jix@ncifcrf.gov) on the NCI-CCR-MCL server (http://mcl1.ncifcrf.gov).