REDDIN KM, CROWLEY-LUKE A, CLARK SO, VINCENT PJ, GORRINGE AR, HUDSON MJ, ROBINSON A; Interscience Conference on Antimicrobial Agents and Chemotherapy (41st : 2001 : Chicago, Ill.).
Abstr Intersci Conf Antimicrob Agents Chemother Intersci Conf Antimicrob Agents Chemother. 2001 Dec 16-19; 41: abstract no. G-1711.
Centre for Applied Microbiology and Research, Salisbury, United Kingdom
BACKGROUND: Serogroup C meningococcal conjugate vaccines generally use diphtheria or tetanus toxoids as protein carriers. This does not broaden protection afforded by the vaccine, and reduced antibody responses to the polysaccharide moiety have been observed in mice primed with carrier. The use of alternative carrier proteins may allow multivalent conjugate vaccines to be formulated into a single injection and reduce the potential for immune suppression in primed individuals. In this study Bordetella pertussis fimbriae were assessed as carrier proteins for Neisseria meningitidis serogroup C polysaccharide (MenCPs). METHODS: Dissociated fimbriae were conjugated to MenCPs using modifications of established methods for reductive amination and carbodiimide-mediated coupling. Conjugates were characterised and purified by size exclusion chromatography and assessed for immunogenicity. RESULTS: Co-elution of protein and polysaccharide moieties confirmed conjugation. In contrast to individual or co-administered antigens, conjugates elicited boostable serum IgG responses to both fimbriae and MenCPs in mice. IgG:IgM ratios confirmed that these responses were thymus-dependent and avidity assay confirmed affinity maturation. High serum bactericidal antibody titres against N. meningitidis serogroup C were observed. In a mouse challenge model, the conjugate vaccine afforded significant protection against lethal infection with N. meningitidis serogroup C (P</=0.01; Finney contingency test). CONCLUSION: B. pertussis fimbriae are effective carrier proteins for MenCPs and could produce a vaccine that protects against meningococcal disease and also augments protection against pertussis, especially where acellular pertussis vaccines deficient in fimbriae are used.
Publication Types:
Keywords:
- Animals
- Antigens, Bacterial
- Bordetella pertussis
- Carrier Proteins
- Diphtheria Toxoid
- Meningococcal Infections
- Meningococcal Vaccines
- Mice
- Pertussis Vaccine
- Tetanus Toxoid
- Vaccines, Conjugate
- Whooping Cough
- immunology
- serogroup C meningococcal conjugate vaccine
Other ID:
UI: 102268557
From Meeting Abstracts