Campbell D, Gentsch J, Papadopoulos T, Janney K, Rifat S, Douglas SD; International Conference on AIDS.
Int Conf AIDS. 1989 Jun 4-9; 5: 301 (abstract no. T.B.P.87).
University of Pennsylvania, Philadelphia, PA, USA
OBJECTIVE: Flow cytometric (FC) methods were developed as potentially improved alternatives to enzyme-linked immunosorbent assays (ELISA) for the detection of HIV specific antibodies, and for the quantitation of HIV proteins. METHODS: Recombinant HIV envelope (ENV-9) protein was covalently coupled to 2.39um caboxylated polystyrene microspheres by carbodiimide mediated reactions and served as the solid phase antigen for both the FC serologic assay and for the FC antigen competition assay. The primary antibody probe for both assays was affinity-purified, biotin-conjugated goat anti-human IgG, while the fluorescence probe was FITC-avidin. RESULTS: The evaluation of forty four human sera by FC and ELISA revealed a high level of correlation (coefficient of correlation = 0.874) between FC mean channel fluorescence and ELISA endpoint titer. The FC antigen competition assay detected less than 50 picograms of recombinant HIV envelope protein. CONCLUSION: The results of the FC HIV antibody detection assay and the FC HIV antigen competition assay demonstrate the potential usefulness of flow cytometry as an alternative method for the quantitation of HIV antibodies and detection of HIV antigens in infected cells.
Publication Types:
Keywords:
- AIDS Serodiagnosis
- AIDS Vaccines
- Acquired Immunodeficiency Syndrome
- Enzyme-Linked Immunosorbent Assay
- Flow Cytometry
- HIV Antibodies
- HIV Antigens
- HIV Seropositivity
- Humans
- Serologic Tests
- immunology
- methods
Other ID:
UI: 102177325
From Meeting Abstracts