Biological Sensors
Nucleic Acid Extraction and Purification (408)
Problem: Need an effective system to pre-process samples for nucleic acid analysis (extraction and clean-up).
Project Activity Scope (from Technical Overview Chart): Mechanisms through Instruments
PNNL Solution:
- Use microbeads with selective surface coatings in a renewable column to capture target analyte
- Use a scalable automated fluidic system for processing microliters to liters of sample
- Automatically perfuse sample and wash solutions to remove inhibitory and interfering compounds
- Elute the concentrated and purified sample suitable for PCR analysis
Capabilities Employed to Meet Client Needs: Optmized fluidics processing protocols, silica and polymer microbeads, reagent optimization, simple automated fluidics system design, sample purification protocols, interferent and inhibitor removal approaches and reagents, polymerase chain reaction (PCR) validation.
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