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Title Nitric oxide mediates glutamate-linked enhancement of cGMP levels in the cerebellum
Creator/Author Bredt, D.S. ; Snyder, S.H. (Johns Hopkins Univ. School of Medicine, Baltimore (USA))
Publication Date1989 Nov 01
OSTI IdentifierOSTI ID: 6957883
Other Number(s)ISSN0027-8424; CODEN: PNASA
Resource TypeJournal Article
Resource RelationProceedings of the National Academy of Sciences of the United States of America ; Vol/Issue: 86:22
Subject550201 -- Biochemistry-- Tracer Techniques; ARGININE-- STOICHIOMETRY;CITRULLINE-- BIOSYNTHESIS;GLUTAMIC ACID-- STOICHIOMETRY;NITRIC OXIDE-- BIOLOGICAL EFFECTS;TRITIUM COMPOUNDS-- BIOSYNTHESIS; ASPARTIC ACID;CEREBELLUM;DOSE-RESPONSE RELATIONSHIPS;GUANOSINE;HEMOGLOBIN;LEUKOCYTES;MUSCLES;NEUROREGULATORS;NUCLEOSIDES;SUPEROXIDE DISMUTASE
Related SubjectAMINO ACIDS;AROMATICS;AUTONOMIC NERVOUS SYSTEM AGENTS;AZAARENES;BIOLOGICAL MATERIALS;BLOOD;BLOOD CELLS;BODY;BODY FLUIDS;BRAIN;CARBOXYLIC ACIDS;CENTRAL NERVOUS SYSTEM;CHALCOGENIDES;DRUGS;ENZYMES;GLOBINS;HETEROCYCLIC ACIDS;HETEROCYCLIC COMPOUNDS;HYDROGEN COMPOUNDS;MATERIALS;NERVOUS SYSTEM;NITROGEN COMPOUNDS;NITROGEN OXIDES;NUCLEOSIDES;NUCLEOTIDES;ORGANIC ACIDS;ORGANIC COMPOUNDS;ORGANIC NITROGEN COMPOUNDS;ORGANS;OXIDES;OXIDOREDUCTASES;OXYGEN COMPOUNDS;PIGMENTS;PORPHYRINS;PROTEINS;PURINES;RIBOSIDES;SYNTHESIS
Description/Abstract Nitric oxide, which mediates influences of numerous neurotransmitters and modulators on vascular smooth muscle and leukocytes, can be formed in the brain from arginine by an enzymatic activity that stoichiometrically generates citrulline.^The authors show that glutamate and related amino acids, such as N-methyl-D-aspartate, markedly stimulate arginine-citrulline transformation in cerebellar slices stoichiometrically with enhancement of cGMP levels.^N{sup {omega}}-monomethyl-L-arginine blocks the augmentation both of citrulline and cGMP with identical potencies.^Arginine competitively reverses both effects of N{sup {omega}}-monomethyl-L-arginine with the same potencies.^Hemoglobin, which complexes nitric oxide, prevents the stimulation by N-methyl-D-aspartate of cGMP levels, and superoxide dismutase, which elevates nitric oxide levels, increases cGMP formation.^These data establish that nitric oxide mediates the stimulation by glutamate of cGMP formation.
Country of PublicationUnited States
LanguageEnglish
FormatPages: 9030-9033
System Entry Date2001 May 13

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