WU-BLAST 2.0 search of the National Center for Biotechnology Information's NR Protein Database.
BEAUTY post-processing provided by the Human Genome Sequencing Center, Baylor College of Medicine.
BEAUTY Reference:
Worley KC, Culpepper P, Wiese BA, Smith RF. BEAUTY-X: enhanced BLAST searches for DNA queries. Bioinformatics 1998;14(10):890-1. Abstract
Worley KC, Wiese BA, Smith RF. BEAUTY: an enhanced BLAST-based search tool that integrates multiple biological information resources into sequence similarity search results. Genome Res 1995 Sep;5(2):173-84 Abstract
RepeatMasker repeats found in sequence:No Repeats Found.Reference: Gish, Warren (1994-1997). unpublished. Gish, Warren and David J. States (1993). Identification of protein coding regions by database similarity search. Nat. Genet. 3:266-72.Notice: statistical significance is estimated under the assumption that the equivalent of one entire reading frame in the query sequence codes for protein and that significant alignments will involve only coding reading frames.
Query= A10D07_CONSENSUS (418 letters)
Translating both strands of query sequence in all 6 reading framesDatabase: nr 625,274 sequences; 197,782,623 total letters.Observed Numbers of Database Sequences Satisfying Various EXPECTation Thresholds (E parameter values) Histogram units: = 3 Sequences : less than 3 sequences EXPECTation Threshold (E parameter) | V Observed Counts--> 10000 916 173 |========================================================= 6310 743 115 |====================================== 3980 628 122 |======================================== 2510 506 129 |=========================================== 1580 377 83 |=========================== 1000 294 45 |=============== 631 249 44 |============== 398 205 30 |========== 251 175 28 |========= 158 147 7 |== 100 140 15 |===== 63.1 125 3 |= 39.8 122 3 |= 25.1 119 3 |= 15.8 116 5 |= >>>>>>>>>>>>>>>>>>>>> Expect = 10.0, Observed = 111 <<<<<<<<<<<<<<<<< 10.0 111 4 |= 6.31 107 0 | 3.98 107 0 | 2.51 107 1 |: 1.58 106 3 |= 1.00 103 3 |= 0.63 100 0 | 0.40 100 2 |: 0.25 98 1 |: 0.16 97 0 | 0.10 97 1 |: 0.063 96 3 |= 0.040 93 3 |= 0.025 90 1 |: 0.016 89 2 |: 0.010 87 1 |: 0.0063 86 1 |: Smallest Sum Reading High Probability Sequences producing High-scoring Segment Pairs: Frame Score P(N) N gi|11273665|pir||JC731120K protein - soybean >gi|5381... +3 548 6.3e-52 1 gi|1071924|pir||S49196Kunitz trypsin inhibitor precur... +3 512 4.1e-48 1 gi|125722|sp|P25272|KTI1_SOYBNKUNITZ-TYPE TRYPSIN INH... +3 310 1.0e-26 1 gi|81979|pir||JS0650chymotrypsin inhibitor (Kunitz) W... +3 285 4.7e-24 1 gi|124150|sp|P10822|ICW3_PSOTECHYMOTRYPSIN INHIBITOR ... +3 285 4.7e-24 1 gi|125723|sp|P25273|KTI2_SOYBNKUNITZ-TYPE TRYPSIN INH... +3 282 9.7e-24 1 gi|81981|pir||JS0651chymotrypsin inhibitor (Kunitz) W... +3 282 9.7e-24 1 gi|2144584|pir||TISYtrypsin inhibitor A (Kunitz) prec... +3 281 1.2e-23 1 gi|99957|pir||S19190trypsin inhibitor B (Kunitz) prec... +3 272 1.1e-22 1 gi|125020|sp|P01070|ITRA_SOYBNTRYPSIN INHIBITORS A/C ... +3 268 3.0e-22 1 gi|2129823|pir||JC4990chymotrypsin inhibitor ECI prec... +3 266 4.8e-22 1 gi|7638034|gb|AAF65315.1|AF233296_1(AF233296) kunitz ... +3 257 4.3e-21 1 gi|2982148|pdb|2WBC| Refined Crystal Structure (2.3... +3 226 8.3e-18 1 gi|8569421|pdb|1EYL|AChain A, Structure Of A Recombin... +3 226 8.3e-18 1 gi|7438247|pir||JC5562trypsin inhibitor ETIb precurso... +3 222 2.2e-17 1 gi|462427|sp|P24924|ITRY_ACACOTRYPSIN INHIBITOR PRECU... +3 218 5.9e-17 1 gi|299509|gb|AAB26177.1|Kunitz-type trypsin inhibitor... +3 218 5.9e-17 1 gi|265716|gb|AAB25433.1|chymotrypsin inhibitor, ECI [... +3 216 9.6e-17 1 gi|462388|sp|P34952|IECI_ERYVACHYMOTRYPSIN INHIBITOR ... +3 216 9.6e-17 1 gi|417176|sp|P32733|ID5A_PROJUKUNITZ-TYPE TRYPSIN INH... +3 214 1.6e-16 1 gi|3913945|sp|P81365|IT1B_ERYVATRYPSIN INHIBITOR 1B (... +3 214 1.6e-16 1 gi|348574|pir||JH0781trypsin inhibitor ETIb (Kunitz) ... +3 214 1.6e-16 1 gi|124154|sp|P09943|IDE3_ERYCATRYPSIN INHIBITOR DE-3 ... +3 206 1.1e-15 1 gi|124155|sp|P07475|IDE3_ERYLATRYPSIN INHIBITOR DE-3 ... +3 206 1.1e-15 1 gi|124152|sp|P09941|ID5A_ADEPATRYPSIN INHIBITOR DE5, ... +3 204 1.8e-15 1 gi|225058|prf||1208243Atrypsin isoinhibitor DE5 [Aden... +3 204 1.8e-15 1 gi|3913898|sp|P81366|IDE3_ERYVATRYPSIN INHIBITOR DE-3... +3 204 1.8e-15 1 gi|68821|pir||TISYCtrypsin inhibitor C (Kunitz) - soy... +3 202 2.9e-15 1 gi|3891584|pdb|1AVU| Trypsin Inhibitor From Soybean... +3 201 3.7e-15 1 gi|3891586|pdb|1AVW|BChain B, Complex Porcine Pancrea... +3 201 3.7e-15 1 gi|3891588|pdb|1AVX|BChain B, Complex Porcine Pancrea... +3 201 3.7e-15 1 gi|125023|sp|P01071|ITRB_SOYBNTRYPSIN INHIBITOR B (KU... +3 198 7.7e-15 1 gi|124938|sp|P10821|IT1A_PSOTETRYPSIN INHIBITOR 1A (W... +3 191 4.3e-14 1 gi|417198|sp|P32877|IT1B_PSOTETRYPSIN INHIBITOR 1B (W... +3 190 5.4e-14 1 gi|625261|pir||A61433trypsin inhibitor 2a (Kunitz) - ... +3 189 7.0e-14 1 gi|9665064|gb|AAF97266.1|AC034106_9(AC034106) Contain... +3 181 4.9e-13 1 gi|124940|sp|P25700|IT2_PSOTETRYPSIN INHIBITOR 2 (WTI... +3 171 5.6e-12 1 gi|2129874|pir||S65830alpha fucosidase precursor - ga... +3 169 9.1e-12 1 gi|81982|pir||JQ0949proteinase inhibitor nodulin prec... +3 159 1.0e-10 1 gi|7438249|pir||T03803tumor-related protein, clone NF... +3 159 1.0e-10 1 gi|3650368|emb|CAA09607.1|(AJ011398) profucosidase [P... +3 159 1.0e-10 1 gi|3859063|emb|CAA57931.1|(X82595) alpha-fucosidase [... +3 159 1.0e-10 1 gi|1076518|pir||S49578trypsin inhibitor homolog - gar... +3 156 2.2e-10 1 gi|5689162|dbj|BAA82840.1|(AB023648) miraculin homolo... +3 153 4.5e-10 1 gi|7438248|pir||T07871miraculin homolog, root-knot ne... +3 142 6.6e-09 1 gi|12324312|gb|AAG52121.1|AC010556_3(AC010556) putati... +3 141 8.5e-09 1 gi|6166552|sp|P13087|MIRA_RICDUMIRACULIN PRECURSOR (M... +3 138 1.8e-08 1 gi|543616|pir||JX0310kunitz type subtilisin inhibitor... +3 137 2.3e-08 1 gi|543617|pir||JX0311kunitz type subtilisin inhibitor... +3 137 2.3e-08 1 gi|5689168|dbj|BAA82843.1|(AB023651) miraculin homolo... +3 136 2.9e-08 1 Locally-aligned regions (HSPs) with respect to query sequence: Locus_ID Frame 3 Hits gi|11273665 | ______________________________________________ gi|1071924 | ______________________________________________ gi|125722 | ___________________________________________ gi|81979 | _________________________________________ gi|124150 | _________________________________________ gi|125723 | ___________________________________________ gi|81981 | _________________________________________ gi|2144584 | __________________________________________ gi|99957 | __________________________________________ gi|125020 | __________________________________________ gi|2129823 | ______________________________________________ gi|7638034 | __________________________________________ gi|2982148 | ________________________________ gi|8569421 | ________________________________ gi|7438247 | _______________________________________ gi|462427 | ____________________________________ gi|299509 | ____________________________________ gi|265716 | ____________________________________ gi|462388 | ____________________________________ gi|417176 | ______________________________ gi|3913945 | _______________________________ gi|348574 | _______________________________ gi|124154 | ______________________________________ gi|124155 | ______________________________________ gi|124152 | ______________________________ gi|225058 | ______________________________ gi|3913898 | ______________________________________ gi|68821 | _________________________________ gi|3891584 | _________________________________ gi|3891586 | _________________________________ gi|3891588 | _________________________________ gi|125023 | _________________________________ gi|124938 | _______________________________ gi|417198 | _______________________________ gi|625261 | _______________________________ gi|9665064 | ____________________________________ gi|124940 | _______________________________ gi|2129874 | _____________________________________ gi|81982 | ________________________ gi|7438249 | _________________________________________ gi|3650368 | ________________________________________ gi|3859063 | _________________________________________ gi|1076518 | _____________________________________ gi|5689162 | _______________________________ gi|7438248 | ______________________________________ gi|12324312 | _____________________________ gi|6166552 | ____________________________________________ gi|543616 | _______________________ gi|543617 | _______________________ gi|5689168 | _____________________________ Prosite Hits: ______ __________________________________________________ Query sequence: | | | | 140 0 50 100 __________________ Prosite hits: SOYBEAN_KUNITZ Soybean trypsin inhibitor (Kunitz) prote 38..54 __________________
Use the
and
icons to retrieve links to Entrez:
WARNING: Descriptions of 61 database sequences were not reported due to the limiting value of parameter V = 50.>gi|11273665|pir||JC7311 20K protein - soybean >gi|5381209|dbj|BAA82254.1| (AB029441) trypsin inhibitor p20 [Glycine max] Length = 206 Frame 3 hits (HSPs): _______________________________ __________________________________________________ Database sequence: | | | | | | 206 0 50 100 150 200 Plus Strand HSPs: Score = 548 (192.9 bits), Expect = 6.3e-52, P = 6.3e-52 Identities = 111/126 (88%), Positives = 114/126 (90%), Frame = +3 Query: 33 MKSTTSLALFLLCALTSSYQPSATADIVFDXEGNPIRNGGTYYVLPVIRGKGGGIXFAKT 212 MKSTTS+ALFLLCALTSSY PS TADIVFD EGNPIRNGGTYYVLPVIRGKGGGI FAKT Sbjct: 1 MKSTTSVALFLLCALTSSYLPS-TADIVFDTEGNPIRNGGTYYVLPVIRGKGGGIEFAKT 59 Query: 213 ETETCPLTVVQSPFEVSKGLPLIISSPFKILDITXGLILSLSFTYVTPCASTPSSVERYX 392 ETETCPLTVVQSPFEVSKGLPLIISSPFKILDIT GLILSLSFTYV PCASTPS Sbjct: 60 ETETCPLTVVQSPFEVSKGLPLIISSPFKILDITEGLILSLSFTYVPPCASTPSRWTVIL 119 Query: 393 KGLPKK 410 KGLP++ Sbjct: 120 KGLPEE 125
>gi|1071924|pir||S49196 Kunitz trypsin inhibitor precursor - soybean >gi|510515|emb|CAA56343.1| (X80039) Kunitz trypsin inhibitor [Glycine max] Length = 208 Frame 3 hits (HSPs): _______________________________ Annotated Domains: ________________________________________________ __________________________________________________ Database sequence: | | | | | | 208 0 50 100 150 200 __________________ Annotated Domains: DOMO DM03168: TRYPSININHIBITOR(KUNITZ) 1..21 DOMO DM00338: SOYBEANTRYPSININHIBITOR(KUNITZ) 23..199 PROSITE SOYBEAN_KUNITZ: Soybean trypsin inhibito 28..44 __________________ Plus Strand HSPs: Score = 512 (180.2 bits), Expect = 4.1e-48, P = 4.1e-48 Identities = 103/127 (81%), Positives = 110/127 (86%), Frame = +3 Query: 33 MKSTTSLALFLLCALTSSYQPSATADIVFDXEGNPIRNGGTYYVLPVIRGKGGGIXFAKT 212 MKSTTSLALFLLCALTSSYQPSATADIVFD EGNPIRNGGTYYVLPVIRGKGGGI FAKT Sbjct: 1 MKSTTSLALFLLCALTSSYQPSATADIVFDTEGNPIRNGGTYYVLPVIRGKGGGIEFAKT 60 Query: 213 ETETCPLTVVQSPFE-VSKGLPLIISSPFKILDITXGLILSLSFTYVTPCASTPSSVERY 389 ETETCPLTVVQSPFE + +GLPLIISSPFKILDIT GLILSL F TP + SV+RY Sbjct: 61 ETETCPLTVVQSPFEGLQRGLPLIISSPFKILDITEGLILSLKFHLCTPLSLNSFSVDRY 120 Query: 390 XKGLPKK 410 +G ++ Sbjct: 121 SQGSARR 127
>gi|125722|sp|P25272|KTI1_SOYBN KUNITZ-TYPE TRYPSIN INHIBITOR KTI1 PRECURSOR >gi|81814|pir||JQ1091 trypsin inhibitor KTi1 (Kunitz) - soybean >gi|256635|gb|AAB23482.1| (S45035) Kunitz trypsin inhibitor KTi1 [soybeans, Peptide, 203 aa] [Glycine max] Length = 203 Frame 3 hits (HSPs): _______________________________ Annotated Domains: __________________________________________________ __________________________________________________ Database sequence: | | | | || 203 0 50 100 150 200 __________________ Annotated Domains: BLOCKS BL00283A: Soybean trypsin inhibitor (Kun 27..47 BLOCKS BL00283B: Soybean trypsin inhibitor (Kun 65..79 BLOCKS BL00283C: Soybean trypsin inhibitor (Kun 152..160 BLOCKS BL00283D: Soybean trypsin inhibitor (Kun 168..177 DOMO DM03168: TRYPSININHIBITOR(KUNITZ) 1..21 DOMO DM00338: SOYBEANTRYPSININHIBITOR(KUNITZ) 23..197 PFAM Kunitz_legume: Trypsin and protease inhi 27..197 PRINTS KUNITZINHBTR1: Kunitz-type STI motif I - 27..56 PRINTS KUNITZINHBTR2: Kunitz-type STI motif II 65..85 PRINTS KUNITZINHBTR3: Kunitz-type STI motif III 141..160 PRINTS KUNITZINHBTR4: Kunitz-type STI motif IV 166..195 PRODOM PD032704: KTI1(1) KTI2(1) 1..24 PRODOM PD000891: IDE3(3) IAAS(3) IT1B(2) 26..163 PRODOM PD010854: 165..202 PROSITE SOYBEAN_KUNITZ: Soybean trypsin inhibito 28..44 __________________ Plus Strand HSPs: Score = 310 (109.1 bits), Expect = 1.0e-26, P = 1.0e-26 Identities = 67/124 (54%), Positives = 81/124 (65%), Frame = +3 Query: 33 MKSTTSLALFLLCALTSSYQPSATADIVFDXEGNPIRNGGTYYVLPVIRGKGGGIXFAKT 212 MKST ALFL+CA T SY PSATA V D + +P++NGGTYY+LPV+RGKGGGI T Sbjct: 1 MKSTIFFALFLVCAFTISYLPSATAQFVLDTDDDPLQNGGTYYMLPVMRGKGGGIEVDST 60 Query: 213 ETETCPLTVVQSPFEVSKGLPLIISSPFKILDITXGLILSL---SFTYVTPCASTPSS-- 377 E CPLTVVQSP E+ KG+ L+ +SP L I LS+ SF +T CA P+ Sbjct: 61 GKEICPLTVVQSPNELDKGIGLVFTSPLHALFIAERYPLSIKFGSFAVITLCAGMPTEWA 120 Query: 378 -VER 386 VER Sbjct: 121 IVER 124
>gi|81979|pir||JS0650 chymotrypsin inhibitor (Kunitz) WCI-2 precursor - winged bean >gi|248875|gb|AAC60536.1| (S96733) Kunitz chymotrypsin inhibitor 2, WCI-2 [Psophocarpus tetragonolobus=winged beans, DC., Peptide, 207 aa] >gi|497834|dbj|BAA06512.1| (D31703) putative Kunitz chymotrypsin inhibitor [Psophocarpus tetragonolobus] Length = 207 Frame 3 hits (HSPs): ____________________________ Annotated Domains: ___________ _ __________________________________________________ Database sequence: | | | | | | 207 0 50 100 150 200 __________________ Annotated Domains: Entrez domain: signal sequence 1..24 Entrez region: inhibitory 89..90 PROSITE SOYBEAN_KUNITZ: Soybean trypsin inhibito 28..44 __________________ Plus Strand HSPs: Score = 285 (100.3 bits), Expect = 4.7e-24, P = 4.7e-24 Identities = 65/113 (57%), Positives = 78/113 (69%), Frame = +3 Query: 33 MKSTTSLALFLLCALTSSYQPSATADI-VFDXEGNPIRNGGTYYVLPVIRGKGGGIXFAK 209 MKSTT LALFLL A+ S + PS+TAD + D EGN + NGGTYY+LP I GGGI AK Sbjct: 1 MKSTTFLALFLLSAIIS-HLPSSTADDDLVDAEGNLVENGGTYYLLPHIWAHGGGIETAK 59 Query: 210 TETETCPLTVVQSPFEVSKGLPLIISSPFKILDITXGLILSLSFTYVTPCASTP 371 T E CPLTVV+SP EVSKG P+ ISS F L I G +++L F CA++P Sbjct: 60 TGNEPCPLTVVRSPNEVSKGEPIRISSQFLSLFIPRGSLVALGFANPPSCAASP 113
>gi|124150|sp|P10822|ICW3_PSOTE CHYMOTRYPSIN INHIBITOR 3 PRECURSOR (WCI-3) >gi|81980|pir||JX0206 chymotrypsin inhibitor (Kunitz) WCI-3 precursor - winged bean >gi|632296|pir||S42564 chymotrypsin inhibitor WCI-3 - winged bean >gi|218005|dbj|BAA03084.1| (D13974) Kunitz chymotrypsin inhibitor precursor [Psophocarpus tetragonolobus] >gi|218007|dbj|BAA03085.1| (D13975) WCI-3 [Psophocarpus tetragonolobus] >gi|218009|dbj|BAA03086.1| (D13976) WCI-3 [Psophocarpus tetragonolobus] >gi|248873|gb|AAC60535.1| (S96732) Kunitz chymotrypsin inhibitor 3, WCI-3 [Psophocarpus tetragonolobus=winged beans, DC., Peptide, 207 aa] >gi|226931|prf||1611457A chymotrypsin inhibitor WCI-3 [Psophocarpus tetragonolobus] Length = 207 Frame 3 hits (HSPs): ____________________________ __________________________________________________ Database sequence: | | | | | | 207 0 50 100 150 200 Plus Strand HSPs: Score = 285 (100.3 bits), Expect = 4.7e-24, P = 4.7e-24 Identities = 65/113 (57%), Positives = 78/113 (69%), Frame = +3 Query: 33 MKSTTSLALFLLCALTSSYQPSATADI-VFDXEGNPIRNGGTYYVLPVIRGKGGGIXFAK 209 MKSTT LALFLL A+ S + PS+TAD + D EGN + NGGTYY+LP I GGGI AK Sbjct: 1 MKSTTFLALFLLSAIIS-HLPSSTADDDLVDAEGNLVENGGTYYLLPHIWAHGGGIETAK 59 Query: 210 TETETCPLTVVQSPFEVSKGLPLIISSPFKILDITXGLILSLSFTYVTPCASTP 371 T E CPLTVV+SP EVSKG P+ ISS F L I G +++L F CA++P Sbjct: 60 TGNEPCPLTVVRSPNEVSKGEPIRISSQFLSLFIPRGSLVALGFANPPSCAASP 113
>gi|125723|sp|P25273|KTI2_SOYBN KUNITZ-TYPE TRYPSIN INHIBITOR KTI2 PRECURSOR >gi|81815|pir||JQ1092 trypsin inhibitor KTi2 (Kunitz) - soybean >gi|256636|gb|AAB23483.1| (S45035) Kunitz trypsin inhibitor KTi2 [soybeans, Peptide, 204 aa] [Glycine max] Length = 204 Frame 3 hits (HSPs): _______________________________ Annotated Domains: __________________________________________________ __________________________________________________ Database sequence: | | | | | | 204 0 50 100 150 200 __________________ Annotated Domains: BLOCKS BL00283A: Soybean trypsin inhibitor (Kun 27..47 BLOCKS BL00283B: Soybean trypsin inhibitor (Kun 65..79 BLOCKS BL00283C: Soybean trypsin inhibitor (Kun 153..161 BLOCKS BL00283D: Soybean trypsin inhibitor (Kun 169..178 PFAM Kunitz_legume: Trypsin and protease inhi 27..198 PRINTS KUNITZINHBTR1: Kunitz-type STI motif I - 27..56 PRINTS KUNITZINHBTR2: Kunitz-type STI motif II 65..85 PRINTS KUNITZINHBTR3: Kunitz-type STI motif III 141..160 PRINTS KUNITZINHBTR4: Kunitz-type STI motif IV 167..196 PRODOM PD032704: KTI1(1) KTI2(1) 1..24 PRODOM PD000891: IDE3(3) IAAS(3) IT1B(2) 26..164 PRODOM PD010854: 166..203 PROSITE SOYBEAN_KUNITZ: Soybean trypsin inhibito 28..44 __________________ Plus Strand HSPs: Score = 282 (99.3 bits), Expect = 9.7e-24, P = 9.7e-24 Identities = 63/124 (50%), Positives = 77/124 (62%), Frame = +3 Query: 33 MKSTTSLALFLLCALTSSYQPSATADIVFDXEGNPIRNGGTYYVLPVIRGKGGGIXFAKT 212 MKST ALFL+CA T SY PSATA V D + +P++NGGTYY+LPV+RGK GGI T Sbjct: 1 MKSTIFFALFLVCAFTISYLPSATAQFVLDTDDDPLQNGGTYYMLPVMRGKSGGIEGNST 60 Query: 213 ETETCPLTVVQSPFEVSKGLPLIISSPFKILDITXGLILSL---SFTYVTPCASTPSS-- 377 E CPLTVVQSP + +KG+ L+ SP L I LS+ SF + C P+ Sbjct: 61 GKEICPLTVVQSPNKHNKGIGLVFKSPLHALFIAERYPLSIKFDSFAVIPLCGVMPTKWA 120 Query: 378 -VER 386 VER Sbjct: 121 IVER 124
>gi|81981|pir||JS0651 chymotrypsin inhibitor (Kunitz) WCI-x precursor - winged bean >gi|248877|gb|AAC60537.1| (S96735) Kunitz chymotrypsin inhibitor x, WCI-x [Psophocarpus tetragonolobus=winged beans, DC., Peptide, 207 aa] >gi|497838|dbj|BAA06513.1| (D31707) putative Kunitz chymotrypsin inhibitor-x [Psophocarpus tetragonolobus] Length = 207 Frame 3 hits (HSPs): ____________________________ Annotated Domains: ___________ _ __________________________________________________ Database sequence: | | | | | | 207 0 50 100 150 200 __________________ Annotated Domains: Entrez domain: signal sequence 1..24 Entrez region: inhibitory 89..90 PROSITE SOYBEAN_KUNITZ: Soybean trypsin inhibito 28..44 __________________ Plus Strand HSPs: Score = 282 (99.3 bits), Expect = 9.7e-24, P = 9.7e-24 Identities = 64/113 (56%), Positives = 77/113 (68%), Frame = +3 Query: 33 MKSTTSLALFLLCALTSSYQPSATADI-VFDXEGNPIRNGGTYYVLPVIRGKGGGIXFAK 209 MKST LALFLL A+ S + PS+TAD + D EGN + NGGTYY+LP I GGGI AK Sbjct: 1 MKSTIFLALFLLPAIIS-HLPSSTADHDLVDVEGNLVENGGTYYLLPQITAHGGGIETAK 59 Query: 210 TETETCPLTVVQSPFEVSKGLPLIISSPFKILDITXGLILSLSFTYVTPCASTP 371 T E CPLTVVQSP+EVS G P+ ISS F L I G +++L F CA++P Sbjct: 60 TGNEPCPLTVVQSPYEVSNGEPIRISSLFLSLFIPRGSLVALGFVNPPSCAASP 113
>gi|2144584|pir||TISY trypsin inhibitor A (Kunitz) precursor - soybean >gi|18770|emb|CAA45777.1| (X64447) trypsin inhibitor subtype A [Glycine max] Length = 217 Frame 3 hits (HSPs): ___________________________ Annotated Domains: __________ _ __________________________________________________ Database sequence: | | | | | | 217 0 50 100 150 200 __________________ Annotated Domains: Entrez domain: signal sequence 1..25 Entrez inhibit site: Arg (trypsin) 88 PROSITE SOYBEAN_KUNITZ: Soybean trypsin inhibito 28..44 __________________ Plus Strand HSPs: Score = 281 (98.9 bits), Expect = 1.2e-23, P = 1.2e-23 Identities = 64/116 (55%), Positives = 73/116 (62%), Frame = +3 Query: 33 MKSTTSLALFLLCALTSSYQPSATADIVFDXEGNPIRNGGTYYVLPVIRGKGGGIXFAKT 212 MKST ALFL CA T+SY PSA AD V D EGNP+ NGGTYY+L I GG I A T Sbjct: 1 MKSTIFFALFLFCAFTTSYLPSAIADFVLDNEGNPLENGGTYYILSDITAFGG-IRAAPT 59 Query: 213 ETETCPLTVVQSPFEVSKGLPLIISSPFKILDITXGLILSL---SFTYVTPCASTPS 374 E CPLTVVQS E+ KG+ IISSP++I I G LSL SF + C P+ Sbjct: 60 GNERCPLTVVQSRNELDKGIGTIISSPYRIRFIAEGHPLSLKFDSFAVIMLCVGIPT 116
>gi|99957|pir||S19190 trypsin inhibitor B (Kunitz) precursor - soybean >gi|18772|emb|CAA45778.1| (X64448) trypsin inhibitor subtype B [Glycine max] Length = 217 Frame 3 hits (HSPs): ___________________________ Annotated Domains: ____ __________________________________________________ Database sequence: | | | | | | 217 0 50 100 150 200 __________________ Annotated Domains: PROSITE SOYBEAN_KUNITZ: Soybean trypsin inhibito 28..44 __________________ Plus Strand HSPs: Score = 272 (95.7 bits), Expect = 1.1e-22, P = 1.1e-22 Identities = 63/116 (54%), Positives = 72/116 (62%), Frame = +3 Query: 33 MKSTTSLALFLLCALTSSYQPSATADIVFDXEGNPIRNGGTYYVLPVIRGKGGGIXFAKT 212 MKST ALFL CA T+SY PSA AD V D EGNP+ +GGTYY+L I GG I A T Sbjct: 1 MKSTIFFALFLFCAFTTSYLPSAIADFVLDNEGNPLDSGGTYYILSDITAFGG-IRAAPT 59 Query: 213 ETETCPLTVVQSPFEVSKGLPLIISSPFKILDITXGLILSL---SFTYVTPCASTPS 374 E CPLTVVQS E+ KG+ IISSPF+I I G L L SF + C P+ Sbjct: 60 GNERCPLTVVQSRNELDKGIGTIISSPFRIRFIAEGNPLRLKFDSFAVIMLCVGIPT 116
>gi|125020|sp|P01070|ITRA_SOYBN TRYPSIN INHIBITORS A/C PRECURSOR (KUNITZ) >gi|99955|pir||JQ0968 trypsin inhibitor KTi3+ (Kunitz) - soybean >gi|256429|gb|AAB23464.1| (S45092) Kunitz trypsin inhibitor, KTi [soybeans, Dare and Forrest, Peptide, 216 aa] [Glycine max] Length = 216 Frame 3 hits (HSPs): ___________________________ Annotated Domains: ______________________________________________ __________________________________________________ Database sequence: | | | | | | 216 0 50 100 150 200 __________________ Annotated Domains: BLOCKS BL00283A: Soybean trypsin inhibitor (Kun 26..46 BLOCKS BL00283B: Soybean trypsin inhibitor (Kun 63..77 BLOCKS BL00283C: Soybean trypsin inhibitor (Kun 153..161 BLOCKS BL00283D: Soybean trypsin inhibitor (Kun 169..178 DOMO DM03168: TRYPSININHIBITOR(KUNITZ) 1..20 DOMO DM00338: SOYBEANTRYPSININHIBITOR(KUNITZ) 22..199 Entrez active site: REACTIVE BOND (TRYPSIN). 87..88 PFAM Kunitz_legume: Trypsin and protease inhi 26..199 PRINTS KUNITZINHBTR1: Kunitz-type STI motif I - 26..55 PRINTS KUNITZINHBTR2: Kunitz-type STI motif II 63..83 PRINTS KUNITZINHBTR3: Kunitz-type STI motif III 141..160 PRINTS KUNITZINHBTR4: Kunitz-type STI motif IV 168..197 PRODOM PD021317: ITRA(1) Q39898(1) Q39899(1) 1..24 PRODOM PD000891: IDE3(3) IAAS(3) IT1B(2) 26..164 PRODOM PD010854: 166..198 PROSITE SOYBEAN_KUNITZ: Soybean trypsin inhibito 27..43 __________________ Plus Strand HSPs: Score = 268 (94.3 bits), Expect = 3.0e-22, P = 3.0e-22 Identities = 63/115 (54%), Positives = 72/115 (62%), Frame = +3 Query: 33 MKSTTSLALFLLCALTSSYQPSATADIVFDXEGNPIRNGGTYYVLPVIRGKGGGIXFAKT 212 MKST LFL CA T+SY PSA AD V D EGNP+ NGGTYY+L I GG I A T Sbjct: 1 MKSTIFF-LFLFCAFTTSYLPSAIADFVLDNEGNPLENGGTYYILSDITAFGG-IRAAPT 58 Query: 213 ETETCPLTVVQSPFEVSKGLPLIISSPFKILDITXGLILSL---SFTYVTPCASTPS 374 E CPLTVVQS E+ KG+ IISSP++I I G LSL SF + C P+ Sbjct: 59 GNERCPLTVVQSRNELDKGIGTIISSPYRIRFIAEGHPLSLKFDSFAVIMLCVGIPT 115
>gi|2129823|pir||JC4990 chymotrypsin inhibitor ECI precursor - Erythrina variegata Length = 203 Frame 3 hits (HSPs): _______________________________ Annotated Domains: ___________ __________________________________________________ Database sequence: | | | | || 203 0 50 100 150 200 __________________ Annotated Domains: Entrez domain: signal sequence 1..24 PROSITE SOYBEAN_KUNITZ: Soybean trypsin inhibito 27..43 __________________ Plus Strand HSPs: Score = 266 (93.6 bits), Expect = 4.8e-22, P = 4.8e-22 Identities = 63/126 (50%), Positives = 77/126 (61%), Frame = +3 Query: 33 MKSTTSLALFLLCALTSSYQPSATADIVFDXEGNPIRNGGTYYVLPVIRGKGGGIXFAKT 212 M+STT LFL+ A S Y PS+TA + D EGN + NGGTYY+LP I GGGI A+T Sbjct: 1 MRSTTYFVLFLVSAFIS-YLPSSTAQPLLDVEGNLVENGGTYYLLPHIWALGGGIEAART 59 Query: 213 ETETCPLTVVQSPFEVSKGLPLIISSPFKILDITXGLILSLSFTYVTPCASTPS-SVERY 389 ETCPLTVVQSPFEVS G P+ I+S F I G ++ F CA+ P +V Sbjct: 60 GKETCPLTVVQSPFEVSNGEPIRIASQFLSTFIPDGSPVAPGFANPPSCAALPWWTVVET 119 Query: 390 XKGLPKK 410 +GL K Sbjct: 120 LRGLAVK 126
>gi|7638034|gb|AAF65315.1|AF233296_1 (AF233296) kunitz trypsin inhibitor 3 [Glycine max] Length = 216 Frame 3 hits (HSPs): ___________________________ __________________________________________________ Database sequence: | | | | | | 216 0 50 100 150 200 Plus Strand HSPs: Score = 257 (90.5 bits), Expect = 4.3e-21, P = 4.3e-21 Identities = 62/115 (53%), Positives = 71/115 (61%), Frame = +3 Query: 33 MKSTTSLALFLLCALTSSYQPSATADIVFDXEGNPIRNGGTYYVLPVIRGKGGGIXFAKT 212 MKST LFL CA T+SY PSA AD V D EGNP+ NGGTYY+L I GG I A T Sbjct: 1 MKSTIFF-LFLFCAFTTSYLPSAIADFVLDNEGNPLENGGTYYILSDITAFGG-IRAAPT 58 Query: 213 ETETCPLTVVQSPFEVSKGLPLIISSPFKILDITXGLILSL---SFTYVTPCASTPS 374 E PLTVVQS E+ KG+ IISSP++I I G LSL SF + C P+ Sbjct: 59 GNERYPLTVVQSRNELDKGIGTIISSPYRIRFIAEGHPLSLKFDSFAVIMLCVGIPT 115
>gi|2982148|pdb|2WBC| Refined Crystal Structure (2.3 Angstrom) Of A Winged Bean Chymotrypsin Inhibitor And Location Of Its Second Reactive Site >gi|1431754|pdb|1WBC| Mol_id: 1; Molecule: Chymotrypsin Inhibitor (Wci); Chain: Null >gi|4699858|pdb|4WBC|A Chain A, 2.13 A Structure Of A Kunitz-Type Winged Bean Chymotrypsin Inhibitor Protein >gi|227379|prf||1703237A Kunitz type chymotrypsin inhibitor [Psophocarpus tetragonolobus] >gi|362443|prf||1413277A chymotrypsin inhibitor WCI3 [Psophocarpus tetragonolobus] Length = 183 Frame 3 hits (HSPs): _________________________ __________________________________________________ Database sequence: | | | | | 183 0 50 100 150 Plus Strand HSPs: Score = 226 (79.6 bits), Expect = 8.3e-18, P = 8.3e-18 Identities = 49/88 (55%), Positives = 59/88 (67%), Frame = +3 Query: 108 DIVFDXEGNPIRNGGTYYVLPVIRGKGGGIXFAKTETETCPLTVVQSPFEVSKGLPLIIS 287 D+V D EGN + NGGTYY+LP I GGGI AKT E CPLTVV+SP EVSKG P+ IS Sbjct: 3 DLV-DAEGNLVENGGTYYLLPHIWAHGGGIETAKTGNEPCPLTVVRSPNEVSKGEPIRIS 61 Query: 288 SPFKILDITXGLILSLSFTYVTPCASTP 371 S F L I G +++L F CA++P Sbjct: 62 SQFLSLFIPRGSLVALGFANPPSCAASP 89
>gi|8569421|pdb|1EYL|A Chain A, Structure Of A Recombinant Winged Bean Chymotrypsin Inhibitor Length = 186 Frame 3 hits (HSPs): ________________________ __________________________________________________ Database sequence: | | | | | 186 0 50 100 150 Plus Strand HSPs: Score = 226 (79.6 bits), Expect = 8.3e-18, P = 8.3e-18 Identities = 49/88 (55%), Positives = 59/88 (67%), Frame = +3 Query: 108 DIVFDXEGNPIRNGGTYYVLPVIRGKGGGIXFAKTETETCPLTVVQSPFEVSKGLPLIIS 287 D+V D EGN + NGGTYY+LP I GGGI AKT E CPLTVV+SP EVSKG P+ IS Sbjct: 6 DLV-DAEGNLVENGGTYYLLPHIWAHGGGIETAKTGNEPCPLTVVRSPNEVSKGEPIRIS 64 Query: 288 SPFKILDITXGLILSLSFTYVTPCASTP 371 S F L I G +++L F CA++P Sbjct: 65 SQFLSLFIPRGSLVALGFANPPSCAASP 92
>gi|7438247|pir||JC5562 trypsin inhibitor ETIb precursor - Erythrina variegata Length = 201 Frame 3 hits (HSPs): ___________________________ __________________________________________________ Database sequence: | | | | || 201 0 50 100 150 200 Plus Strand HSPs: Score = 222 (78.1 bits), Expect = 2.2e-17, P = 2.2e-17 Identities = 49/107 (45%), Positives = 65/107 (60%), Frame = +3 Query: 51 LALFLLCALTSSYQPSATADIVFDXEGNPIRNGGTYYVLPVIRGKGGGIXFAKTETETCP 230 + LF++ + S S T +V D EG + NGGTYY+LP I G GGG+ AKT ETCP Sbjct: 5 IVLFIVSSFFSFLHLS-TGQLV-DVEGEDVVNGGTYYMLPGIEGDGGGMEGAKTGRETCP 62 Query: 231 LTVVQSPFEVSKGLPLIISSPFKILDITXGLILSLSFTYVTPCASTP 371 +TVVQS +VS G P+ I SPF+ I G ++ + FT CA +P Sbjct: 63 ITVVQSRNDVSNGEPITIESPFRSYFIPKGSLVRIGFTSPPKCAPSP 109
>gi|462427|sp|P24924|ITRY_ACACO TRYPSIN INHIBITOR PRECURSOR >gi|81736|pir||JH0607 trypsin inhibitor (Kunitz) precursor - Acacia confusa >gi|166234|gb|AAA32618.1| (M92852) trypsin inhibitor [Acacia confusa] Length = 176 Frame 3 hits (HSPs): ___________________________ Annotated Domains: __________________________________________________ __________________________________________________ Database sequence: | | | | | 176 0 50 100 150 __________________ Annotated Domains: BLOCKS BL00283A: Soybean trypsin inhibitor (Kun 2..22 BLOCKS BL00283B: Soybean trypsin inhibitor (Kun 40..54 BLOCKS BL00283C: Soybean trypsin inhibitor (Kun 126..134 BLOCKS BL00283D: Soybean trypsin inhibitor (Kun 142..151 DOMO DM00338: SOYBEANTRYPSININHIBITOR(KUNITZ) 1..171 PFAM Kunitz_legume: Trypsin and protease inhi 2..171 PRODOM PD000891: IDE3(3) IAAS(3) IT1B(2) 1..133 PRODOM PD018852: ID5B(2) ITRY(1) 142..175 PROSITE SOYBEAN_KUNITZ: Soybean trypsin inhibito 3..19 __________________ Plus Strand HSPs: Score = 218 (76.7 bits), Expect = 5.9e-17, P = 5.9e-17 Identities = 45/99 (45%), Positives = 63/99 (63%), Frame = +3 Query: 114 VFDXEGNPIRNGGTYYVLPVIRGKGGGIXFAKTETETCPLTVVQSPFEVSKGLPLIISSP 293 + D +G+ +RNGG YY+LP +RGKGGG+ AKT E+CPLTVVQ+ E +GLP +I +P Sbjct: 3 LLDADGDILRNGGAYYILPALRGKGGGLTLAKTGDESCPLTVVQAQSETKRGLPAVIWTP 62 Query: 294 FKILDITXGLILSLSFTYVTPCASTPSSVERYXKGLPKK 410 KI +T G L+ F P P+ +++Y LP K Sbjct: 63 PKIAILTPGFYLNFEFQ---P-RDLPACLQKYST-LPWK 96
>gi|299509|gb|AAB26177.1| Kunitz-type trypsin inhibitor A chain, ACTI-A [Acacia confusa, seeds, Peptide, 136 aa] Length = 136 Frame 3 hits (HSPs): ___________________________________ Annotated Domains: _______ __________________________________________________ Database sequence: | | | | 136 0 50 100 __________________ Annotated Domains: PROSITE SOYBEAN_KUNITZ: Soybean trypsin inhibito 3..19 __________________ Plus Strand HSPs: Score = 218 (76.7 bits), Expect = 5.9e-17, P = 5.9e-17 Identities = 45/99 (45%), Positives = 63/99 (63%), Frame = +3 Query: 114 VFDXEGNPIRNGGTYYVLPVIRGKGGGIXFAKTETETCPLTVVQSPFEVSKGLPLIISSP 293 + D +G+ +RNGG YY+LP +RGKGGG+ AKT E+CPLTVVQ+ E +GLP +I +P Sbjct: 3 LLDADGDILRNGGAYYILPALRGKGGGLTLAKTGDESCPLTVVQAQSETKRGLPAVIWTP 62 Query: 294 FKILDITXGLILSLSFTYVTPCASTPSSVERYXKGLPKK 410 KI +T G L+ F P P+ +++Y LP K Sbjct: 63 PKIAILTPGFYLNFEFQ---P-RDLPACLQKYST-LPWK 96
>gi|265716|gb|AAB25433.1| chymotrypsin inhibitor, ECI [Erythrina variegata, var. Orientalis, seeds, Peptide, 179 aa] >gi|444800|prf||1908233A chymotrypsin inhibitor ECI [Erythrina variegata] Length = 179 Frame 3 hits (HSPs): ____________________________ Annotated Domains: ______ __________________________________________________ Database sequence: | | | | | 179 0 50 100 150 __________________ Annotated Domains: PROSITE SOYBEAN_KUNITZ: Soybean trypsin inhibito 3..19 __________________ Plus Strand HSPs: Score = 216 (76.0 bits), Expect = 9.6e-17, P = 9.6e-17 Identities = 49/98 (50%), Positives = 60/98 (61%), Frame = +3 Query: 120 DXEGNPIRNGGTYYVLPVIRGKGGGIXFAKTETETCPLTVVQSPFEVSKGLPLIISSPFK 299 D EGN + NGGTYY+LP I GGGI A+T ETCPLTVVQSPFEVS G P+ I+S F Sbjct: 5 DLEGNLVENGGTYYLLPHIWALGGGIEAARTGKETCPLTVVQSPFEVSNGEPIRIASQFL 64 Query: 300 ILDITXGLILSLSFTYVTPCASTPS-SVERYXKGLPKK 410 I G ++ F CA++P +V +GL K Sbjct: 65 STFIPDGSPYAIGFANPPSCAASPWWTVVETSEGLAVK 102
>gi|462388|sp|P34952|IECI_ERYVA CHYMOTRYPSIN INHIBITOR ECI >gi|418814|pir||JC1487 chymotrypsin inhibitor (Kunitz) ECI - Erythrina variegata var. orientalis Length = 179 Frame 3 hits (HSPs): ____________________________ Annotated Domains: __________________________________________________ __________________________________________________ Database sequence: | | | | | 179 0 50 100 150 __________________ Annotated Domains: BLOCKS BL00283A: Soybean trypsin inhibitor (Kun 2..22 BLOCKS BL00283B: Soybean trypsin inhibitor (Kun 40..54 BLOCKS BL00283C: Soybean trypsin inhibitor (Kun 127..135 BLOCKS BL00283D: Soybean trypsin inhibitor (Kun 144..153 DOMO DM00338: SOYBEANTRYPSININHIBITOR(KUNITZ) 1..173 Entrez pyrrolidone-carboxylic-acid site 1 Entrez active site: REACTIVE BOND (CHYMOTRYPSIN 64..65 PFAM Kunitz_legume: Trypsin and protease inhi 2..173 PRINTS KUNITZINHBTR1: Kunitz-type STI motif I - 2..31 PRINTS KUNITZINHBTR2: Kunitz-type STI motif II 40..60 PRINTS KUNITZINHBTR3: Kunitz-type STI motif III 115..134 PRINTS KUNITZINHBTR4: Kunitz-type STI motif IV 142..171 PRODOM PD000891: IDE3(3) IAAS(3) IT1B(2) 1..135 PRODOM PD016102: ICW3(1) Q43708(1) Q43721(1) 140..177 PROSITE SOYBEAN_KUNITZ: Soybean trypsin inhibito 3..19 __________________ Plus Strand HSPs: Score = 216 (76.0 bits), Expect = 9.6e-17, P = 9.6e-17 Identities = 49/98 (50%), Positives = 60/98 (61%), Frame = +3 Query: 120 DXEGNPIRNGGTYYVLPVIRGKGGGIXFAKTETETCPLTVVQSPFEVSKGLPLIISSPFK 299 D EGN + NGGTYY+LP I GGGI A+T ETCPLTVVQSPFEVS G P+ I+S F Sbjct: 5 DLEGNLVENGGTYYLLPHIWALGGGIEAARTGKETCPLTVVQSPFEVSNGEPIRIASQFL 64 Query: 300 ILDITXGLILSLSFTYVTPCASTPS-SVERYXKGLPKK 410 I G ++ F CA++P +V +GL K Sbjct: 65 STFIPDGSPYAIGFANPPSCAASPWWTVVETSEGLAVK 102
>gi|417176|sp|P32733|ID5A_PROJU KUNITZ-TYPE TRYPSIN INHIBITOR ALPHA CHAIN >gi|348463|pir||B45588 kunitz trypsin inhibitor alpha chain - Prosopsis juliflora >gi|243387|gb|AAB21123.1| Kunitz trypsin inhibitor alpha chain [Prosopsis juliflora, seeds, Peptide, 137 aa] Length = 137 Frame 3 hits (HSPs): _______________________________ Annotated Domains: __________________________________________________ __________________________________________________ Database sequence: | | | | 137 0 50 100 __________________ Annotated Domains: DOMO DM00338: SOYBEANTRYPSININHIBITOR(KUNITZ) 1..136 Entrez active site: REACTIVE BOND (TRYPSIN). 64..65 PFAM Kunitz_legume: Trypsin and protease inhi 2..136 PRODOM PD000891: IDE3(3) IAAS(3) IT1B(2) 1..134 PROSITE SOYBEAN_KUNITZ: Soybean trypsin inhibito 3..19 __________________ Plus Strand HSPs: Score = 214 (75.3 bits), Expect = 1.6e-16, P = 1.6e-16 Identities = 43/83 (51%), Positives = 54/83 (65%), Frame = +3 Query: 114 VFDXEGNPIRNGGTYYVLPVIRGKGGGIXFAKTETETCPLTVVQSPFEVSKGLPLIISSP 293 + D +G +RNGG+YY+LP RGKGGG+ AKTE ETCPLTVVQ+ E +GLP I SP Sbjct: 3 LLDVDGEILRNGGSYYILPAFRGKGGGLELAKTEGETCPLTVVQARSETDRGLPASIWSP 62 Query: 294 FKILDITXGLILSLSFTYVTPCA 362 +I I G L++ F P A Sbjct: 63 PRIAIIRPGFSLNIEFRPRNPSA 85
>gi|3913945|sp|P81365|IT1B_ERYVA TRYPSIN INHIBITOR 1B (ETIB) >gi|262753|gb|AAB24755.1| ETIb=Kunitz-type trypsin inhibitor [Erythrina variegata, var. Orientalis, seeds, Peptide, 176 aa] >gi|383379|prf||1903155B trypsin inhibitor ET1b [Erythrina variegata orientalis] Length = 176 Frame 3 hits (HSPs): _________________________ Annotated Domains: __________________________________________________ __________________________________________________ Database sequence: | | | | | 176 0 50 100 150 __________________ Annotated Domains: Entrez active site: REACTIVE BOND (TRYPSIN) (BY 63..64 PFAM Kunitz_legume: Trypsin and protease inhi 1..172 PRINTS KUNITZINHBTR1: Kunitz-type STI motif I - 1..30 PRINTS KUNITZINHBTR2: Kunitz-type STI motif II 39..59 PRINTS KUNITZINHBTR3: Kunitz-type STI motif III 114..133 PRINTS KUNITZINHBTR4: Kunitz-type STI motif IV 141..170 PRODOM PD000891: IDE3(3) IAAS(3) IT1B(2) 1..135 PRODOM PD009415: IDE3(3) IT1B(2) IT1A(1) 143..175 PROSITE SOYBEAN_KUNITZ: Soybean trypsin inhibito 2..18 __________________ Plus Strand HSPs: Score = 214 (75.3 bits), Expect = 1.6e-16, P = 1.6e-16 Identities = 43/84 (51%), Positives = 54/84 (64%), Frame = +3 Query: 120 DXEGNPIRNGGTYYVLPVIRGKGGGIXFAKTETETCPLTVVQSPFEVSKGLPLIISSPFK 299 D EG + NGGTYY+LP I G GGG+ AKT ETCP+TVVQS +VS G P+ I SPF+ Sbjct: 4 DVEGEDVVNGGTYYMLPGIEGDGGGMEGAKTGRETCPITVVQSRNDVSNGEPITIESPFR 63 Query: 300 ILDITXGLILSLSFTYVTPCASTP 371 I G ++ + FT CA +P Sbjct: 64 SYFIPKGSLVRIGFTSPPKCAPSP 87
>gi|348574|pir||JH0781 trypsin inhibitor ETIb (Kunitz) - Erythrina variegata Length = 176 Frame 3 hits (HSPs): _________________________ Annotated Domains: _________________________________________________ __________________________________________________ Database sequence: | | | | | 176 0 50 100 150 __________________ Annotated Domains: DOMO DM00338: SOYBEANTRYPSININHIBITOR(KUNITZ) 1..172 Entrez modified site: pyrrolidone carboxylic ac 1 PROSITE SOYBEAN_KUNITZ: Soybean trypsin inhibito 2..18 __________________ Plus Strand HSPs: Score = 214 (75.3 bits), Expect = 1.6e-16, P = 1.6e-16 Identities = 43/84 (51%), Positives = 54/84 (64%), Frame = +3 Query: 120 DXEGNPIRNGGTYYVLPVIRGKGGGIXFAKTETETCPLTVVQSPFEVSKGLPLIISSPFK 299 D EG + NGGTYY+LP I G GGG+ AKT ETCP+TVVQS +VS G P+ I SPF+ Sbjct: 4 DVEGEDVVNGGTYYMLPGIEGDGGGMEGAKTGRETCPITVVQSRNDVSNGEPITIESPFR 63 Query: 300 ILDITXGLILSLSFTYVTPCASTP 371 I G ++ + FT CA +P Sbjct: 64 SYFIPKGSLVRIGFTSPPKCAPSP 87
>gi|124154|sp|P09943|IDE3_ERYCA TRYPSIN INHIBITOR DE-3 >gi|81751|pir||A27220 trypsin inhibitor DE-3 (Kunitz) - kaffir tree >gi|230358|pdb|1TIE| Erythrina Trypsin Inhibitor (Kunitz) De-3 Length = 172 Frame 3 hits (HSPs): ______________________________ Annotated Domains: __________________________________________________ __________________________________________________ Database sequence: | | | | | 172 0 50 100 150 __________________ Annotated Domains: BLOCKS BL00283A: Soybean trypsin inhibitor (Kun 1..21 BLOCKS BL00283B: Soybean trypsin inhibitor (Kun 39..53 BLOCKS BL00283C: Soybean trypsin inhibitor (Kun 125..133 BLOCKS BL00283D: Soybean trypsin inhibitor (Kun 139..148 DOMO DM00338: SOYBEANTRYPSININHIBITOR(KUNITZ) 1..168 Entrez active site: REACTIVE BOND (TRYPSIN). 63..64 PFAM Kunitz_legume: Trypsin and protease inhi 1..168 PRINTS KUNITZINHBTR1: Kunitz-type STI motif I - 1..30 PRINTS KUNITZINHBTR2: Kunitz-type STI motif II 39..59 PRINTS KUNITZINHBTR3: Kunitz-type STI motif III 114..133 PRINTS KUNITZINHBTR4: Kunitz-type STI motif IV 137..166 PRODOM PD000891: IDE3(3) IAAS(3) IT1B(2) 2..132 PRODOM PD009415: IDE3(3) IT1B(2) IT1A(1) 135..171 PROSITE SOYBEAN_KUNITZ: Soybean trypsin inhibito 2..18 __________________ Plus Strand HSPs: Score = 206 (72.5 bits), Expect = 1.1e-15, P = 1.1e-15 Identities = 45/104 (43%), Positives = 61/104 (58%), Frame = +3 Query: 111 IVFDXEGNPIRNGGTYYVLPVIRGKGGGIXFAKTETETCPLTVVQSPFEVSKGLPLIISS 290 ++ D G ++NGGTYY+LP + +GGG+ AKT ETCPLTVVQSP E+S G P+ I S Sbjct: 1 VLLDGNGEVVQNGGTYYLLPQVWAQGGGVQLAKTGEETCPLTVVQSPNELSDGKPIRIES 60 Query: 291 PFKILDITXGLILSLSFTYVTPCASTP--SSVERYXKGLPKKNS 416 + I + + F Y CA +P + VE +GL K S Sbjct: 61 RLRSAFIPDDDKVRIGFAYAPKCAPSPWWTVVEDEQEGLSVKLS 104
>gi|124155|sp|P07475|IDE3_ERYLA TRYPSIN INHIBITOR DE-3 >gi|81754|pir||A24082 trypsin inhibitor DE-3 - coral bean Length = 172 Frame 3 hits (HSPs): ______________________________ Annotated Domains: __________________________________________________ __________________________________________________ Database sequence: | | | | | 172 0 50 100 150 __________________ Annotated Domains: BLOCKS BL00283A: Soybean trypsin inhibitor (Kun 1..21 BLOCKS BL00283B: Soybean trypsin inhibitor (Kun 39..53 BLOCKS BL00283C: Soybean trypsin inhibitor (Kun 125..133 BLOCKS BL00283D: Soybean trypsin inhibitor (Kun 139..148 Entrez active site: REACTIVE BOND (TRYPSIN). 63..64 PFAM Kunitz_legume: Trypsin and protease inhi 1..168 PRINTS KUNITZINHBTR1: Kunitz-type STI motif I - 1..30 PRINTS KUNITZINHBTR2: Kunitz-type STI motif II 39..59 PRINTS KUNITZINHBTR3: Kunitz-type STI motif III 114..133 PRINTS KUNITZINHBTR4: Kunitz-type STI motif IV 137..166 PRODOM PD000891: IDE3(3) IAAS(3) IT1B(2) 2..132 PRODOM PD009415: IDE3(3) IT1B(2) IT1A(1) 135..171 PROSITE SOYBEAN_KUNITZ: Soybean trypsin inhibito 2..18 __________________ Plus Strand HSPs: Score = 206 (72.5 bits), Expect = 1.1e-15, P = 1.1e-15 Identities = 45/104 (43%), Positives = 61/104 (58%), Frame = +3 Query: 111 IVFDXEGNPIRNGGTYYVLPVIRGKGGGIXFAKTETETCPLTVVQSPFEVSKGLPLIISS 290 ++ D G ++NGGTYY+LP + +GGG+ AKT ETCPLTVVQSP E+S G P+ I S Sbjct: 1 VLLDGNGEVVQNGGTYYLLPQVWAQGGGVQLAKTGEETCPLTVVQSPNELSDGKPIRIES 60 Query: 291 PFKILDITXGLILSLSFTYVTPCASTP--SSVERYXKGLPKKNS 416 + I + + F Y CA +P + VE +GL K S Sbjct: 61 RLRSTFIPDDDEVRIGFAYAPKCAPSPWWTVVEDEQEGLSVKLS 104
>gi|124152|sp|P09941|ID5A_ADEPA TRYPSIN INHIBITOR DE5, ALPHA CHAIN >gi|81737|pir||A24376 trypsin inhibitor DE5 alpha chain - bead tree Length = 138 Frame 3 hits (HSPs): ______________________________ Annotated Domains: __________________________________________________ __________________________________________________ Database sequence: | | | | 138 0 50 100 __________________ Annotated Domains: DOMO DM00338: SOYBEANTRYPSININHIBITOR(KUNITZ) 1..137 Entrez active site: REACTIVE BOND (TRYPSIN). 64..65 PFAM Kunitz_legume: Trypsin and protease inhi 2..136 PRODOM PD000891: IDE3(3) IAAS(3) IT1B(2) 1..134 PROSITE SOYBEAN_KUNITZ: Soybean trypsin inhibito 3..19 __________________ Plus Strand HSPs: Score = 204 (71.8 bits), Expect = 1.8e-15, P = 1.8e-15 Identities = 39/81 (48%), Positives = 55/81 (67%), Frame = +3 Query: 114 VFDXEGNPIRNGGTYYVLPVIRGKGGGIXFAKTETETCPLTVVQSPFEVSKGLPLIISSP 293 + D +GN +RNGG+YY++P RGKGGG+ A+T +ETCP TVVQ+P E S+GLP +S+P Sbjct: 3 LLDVDGNFLRNGGSYYIVPAFRGKGGGLELARTGSETCPRTVVQAPAEQSRGLPARLSTP 62 Query: 294 FKILDITXGLILSLSFTYVTP 356 +I I L++ F P Sbjct: 63 PRIRYIGPEFYLTIEFEEQKP 83
>gi|225058|prf||1208243A trypsin isoinhibitor DE5 [Adenanthera pavonina] Length = 176 Frame 3 hits (HSPs): ________________________ __________________________________________________ Database sequence: | | | | | 176 0 50 100 150 Plus Strand HSPs: Score = 204 (71.8 bits), Expect = 1.8e-15, P = 1.8e-15 Identities = 39/81 (48%), Positives = 55/81 (67%), Frame = +3 Query: 114 VFDXEGNPIRNGGTYYVLPVIRGKGGGIXFAKTETETCPLTVVQSPFEVSKGLPLIISSP 293 + D +GN +RNGG+YY++P RGKGGG+ A+T +ETCP TVVQ+P E S+GLP +S+P Sbjct: 3 LLDVDGNFLRNGGSYYIVPAFRGKGGGLELARTGSETCPRTVVQAPAEQSRGLPARLSTP 62 Query: 294 FKILDITXGLILSLSFTYVTP 356 +I I L++ F P Sbjct: 63 PRIRYIGPEFYLTIEFEEQKP 83
>gi|3913898|sp|P81366|IDE3_ERYVA TRYPSIN INHIBITOR DE-3 (ETIA) >gi|348573|pir||JH0780 trypsin inhibitor ETIa (Kunitz) - Erythrina variegata >gi|262754|gb|AAB24756.1| ETIa=Kunitz-type trypsin inhibitor [Erythrina variegata, var. Orientalis, seeds, Peptide, 172 aa] >gi|383378|prf||1903155A trypsin inhibitor ET1a [Erythrina variegata orientalis] Length = 172 Frame 3 hits (HSPs): ______________________________ Annotated Domains: __________________________________________________ __________________________________________________ Database sequence: | | | | | 172 0 50 100 150 __________________ Annotated Domains: Entrez active site: REACTIVE BOND (TRYPSIN). 63..64 PFAM Kunitz_legume: Trypsin and protease inhi 1..168 PRINTS KUNITZINHBTR1: Kunitz-type STI motif I - 1..30 PRINTS KUNITZINHBTR2: Kunitz-type STI motif II 39..59 PRINTS KUNITZINHBTR3: Kunitz-type STI motif III 114..133 PRINTS KUNITZINHBTR4: Kunitz-type STI motif IV 137..166 PRODOM PD000891: IDE3(3) IAAS(3) IT1B(2) 2..132 PRODOM PD009415: IDE3(3) IT1B(2) IT1A(1) 135..171 PROSITE SOYBEAN_KUNITZ: Soybean trypsin inhibito 2..18 __________________ Plus Strand HSPs: Score = 204 (71.8 bits), Expect = 1.8e-15, P = 1.8e-15 Identities = 44/104 (42%), Positives = 61/104 (58%), Frame = +3 Query: 111 IVFDXEGNPIRNGGTYYVLPVIRGKGGGIXFAKTETETCPLTVVQSPFEVSKGLPLIISS 290 ++ D G ++NGGTYY+LP + +GGG+ AKT ETCPLTVVQSP E+S G P+ I S Sbjct: 1 VLLDGNGEVVQNGGTYYLLPQVWAQGGGVQLAKTGEETCPLTVVQSPNELSNGKPIRIES 60 Query: 291 PFKILDITXGLILSLSFTYVTPCASTP--SSVERYXKGLPKKNS 416 + I + + F Y CA +P + +E +GL K S Sbjct: 61 RLRSAFIPDDDKVRIGFAYAPKCAPSPWWTVLEDEQEGLSVKLS 104
>gi|68821|pir||TISYC trypsin inhibitor C (Kunitz) - soybean >gi|354136|prf||1108235C inhibitor Tic,trypsin [Glycine max] Length = 181 Frame 3 hits (HSPs): _________________________ Annotated Domains: _____ _ __________________________________________________ Database sequence: | | | | | 181 0 50 100 150 __________________ Annotated Domains: Entrez inhibit site: Arg (trypsin) 63 PROSITE SOYBEAN_KUNITZ: Soybean trypsin inhibito 3..19 __________________ Plus Strand HSPs: Score = 202 (71.1 bits), Expect = 2.9e-15, P = 2.9e-15 Identities = 47/91 (51%), Positives = 55/91 (60%), Frame = +3 Query: 108 DIVFDXEGNPIRNGGTYYVLPVIRGKGGGIXFAKTETETCPLTVVQSPFEVSKGLPLIIS 287 D V D EGNP+ NGGTYY+L I GG I A T E CPLTVVQS E+ KG+ IIS Sbjct: 1 DFVLDNEGNPLENGGTYYILSDITAFGG-IRAAPTGNERCPLTVVQSRNELDKGIETIIS 59 Query: 288 SPFKILDITXGLILSL---SFTYVTPCASTPS 374 SP++I I G LSL SF + C P+ Sbjct: 60 SPYRIRFIAEGHPLSLKFDSFAVIMLCVGIPT 91
>gi|3891584|pdb|1AVU| Trypsin Inhibitor From Soybean (Sti) >gi|3318878|pdb|1BA7|B Chain B, Soybean Trypsin Inhibitor >gi|3318877|pdb|1BA7|A Chain A, Soybean Trypsin Inhibitor >gi|354134|prf||1108235A inhibitor Tia,trypsin [Glycine max] Length = 181 Frame 3 hits (HSPs): _________________________ __________________________________________________ Database sequence: | | | | | 181 0 50 100 150 Plus Strand HSPs: Score = 201 (70.8 bits), Expect = 3.7e-15, P = 3.7e-15 Identities = 47/91 (51%), Positives = 55/91 (60%), Frame = +3 Query: 108 DIVFDXEGNPIRNGGTYYVLPVIRGKGGGIXFAKTETETCPLTVVQSPFEVSKGLPLIIS 287 D V D EGNP+ NGGTYY+L I GG I A T E CPLTVVQS E+ KG+ IIS Sbjct: 1 DFVLDNEGNPLENGGTYYILSDITAFGG-IRAAPTGNERCPLTVVQSRNELDKGIGTIIS 59 Query: 288 SPFKILDITXGLILSL---SFTYVTPCASTPS 374 SP++I I G LSL SF + C P+ Sbjct: 60 SPYRIRFIAEGHPLSLKFDSFAVIMLCVGIPT 91
>gi|3891586|pdb|1AVW|B Chain B, Complex Porcine Pancreatic TrypsinSOYBEAN TRYPSIN Inhibitor, Orthorhombic Crystal Form Length = 171 Frame 3 hits (HSPs): ___________________________ __________________________________________________ Database sequence: | | | | | 171 0 50 100 150 Plus Strand HSPs: Score = 201 (70.8 bits), Expect = 3.7e-15, P = 3.7e-15 Identities = 47/91 (51%), Positives = 55/91 (60%), Frame = +3 Query: 108 DIVFDXEGNPIRNGGTYYVLPVIRGKGGGIXFAKTETETCPLTVVQSPFEVSKGLPLIIS 287 D V D EGNP+ NGGTYY+L I GG I A T E CPLTVVQS E+ KG+ IIS Sbjct: 1 DFVLDNEGNPLENGGTYYILSDITAFGG-IRAAPTGNERCPLTVVQSRNELDKGIGTIIS 59 Query: 288 SPFKILDITXGLILSL---SFTYVTPCASTPS 374 SP++I I G LSL SF + C P+ Sbjct: 60 SPYRIRFIAEGHPLSLKFDSFAVIMLCVGIPT 91
>gi|3891588|pdb|1AVX|B Chain B, Complex Porcine Pancreatic TrypsinSOYBEAN TRYPSIN Inhibitor, Tetragonal Crystal Form Length = 172 Frame 3 hits (HSPs): ___________________________ __________________________________________________ Database sequence: | | | | | 172 0 50 100 150 Plus Strand HSPs: Score = 201 (70.8 bits), Expect = 3.7e-15, P = 3.7e-15 Identities = 47/91 (51%), Positives = 55/91 (60%), Frame = +3 Query: 108 DIVFDXEGNPIRNGGTYYVLPVIRGKGGGIXFAKTETETCPLTVVQSPFEVSKGLPLIIS 287 D V D EGNP+ NGGTYY+L I GG I A T E CPLTVVQS E+ KG+ IIS Sbjct: 1 DFVLDNEGNPLENGGTYYILSDITAFGG-IRAAPTGNERCPLTVVQSRNELDKGIGTIIS 59 Query: 288 SPFKILDITXGLILSL---SFTYVTPCASTPS 374 SP++I I G LSL SF + C P+ Sbjct: 60 SPYRIRFIAEGHPLSLKFDSFAVIMLCVGIPT 91
>gi|125023|sp|P01071|ITRB_SOYBN TRYPSIN INHIBITOR B (KUNITZ) >gi|68823|pir||TISYB trypsin inhibitor B (Kunitz) - soybean >gi|354135|prf||1108235B inhibitor Tib,trypsin [Glycine max] Length = 181 Frame 3 hits (HSPs): _________________________ Annotated Domains: _________________________________________________ __________________________________________________ Database sequence: | | | | | 181 0 50 100 150 __________________ Annotated Domains: BLOCKS BL00283A: Soybean trypsin inhibitor (Kun 2..22 BLOCKS BL00283B: Soybean trypsin inhibitor (Kun 39..53 BLOCKS BL00283C: Soybean trypsin inhibitor (Kun 129..137 BLOCKS BL00283D: Soybean trypsin inhibitor (Kun 145..154 Entrez active site: REACTIVE BOND (TRYPSIN). 63..64 PFAM Kunitz_legume: Trypsin and protease inhi 2..175 PRINTS KUNITZINHBTR1: Kunitz-type STI motif I - 2..31 PRINTS KUNITZINHBTR2: Kunitz-type STI motif II 39..59 PRINTS KUNITZINHBTR3: Kunitz-type STI motif III 117..136 PRINTS KUNITZINHBTR4: Kunitz-type STI motif IV 144..173 PRODOM PD000891: IDE3(3) IAAS(3) IT1B(2) 2..140 PRODOM PD010854: 142..174 PROSITE SOYBEAN_KUNITZ: Soybean trypsin inhibito 3..19 __________________ Plus Strand HSPs: Score = 198 (69.7 bits), Expect = 7.7e-15, P = 7.7e-15 Identities = 47/91 (51%), Positives = 54/91 (59%), Frame = +3 Query: 108 DIVFDXEGNPIRNGGTYYVLPVIRGKGGGIXFAKTETETCPLTVVQSPFEVSKGLPLIIS 287 D V D EGNP+ NGGTYY+L I GG I A T E CPLTVVQS E+ KG+ IIS Sbjct: 1 DFVLDNEGNPLSNGGTYYILSDITAFGG-IRAAPTGNERCPLTVVQSRNELDKGIGTIIS 59 Query: 288 SPFKILDITXGLILSL---SFTYVTPCASTPS 374 SPF+I I G L L SF + C P+ Sbjct: 60 SPFRIRFIAEGNPLRLKFDSFAVIMLCVGIPT 91
>gi|124938|sp|P10821|IT1A_PSOTE TRYPSIN INHIBITOR 1A (WTI-1A) >gi|68824|pir||TIWDK trypsin inhibitor 1A (Kunitz) - winged bean Length = 172 Frame 3 hits (HSPs): __________________________ __________________________________________________ Database sequence: | | | | | 172 0 50 100 150 Plus Strand HSPs: Score = 191 (67.2 bits), Expect = 4.3e-14, P = 4.3e-14 Identities = 43/86 (50%), Positives = 52/86 (60%), Frame = +3 Query: 114 VFDXEGNPIRNGGTYYVLPVIRGKGGGIXFAKTETETCPLTVVQSPFEVSKGLPLIISSP 293 + D EG +RNGGTYY+LP GGGI A T TETCPLTVV+SP EVS G PL ISS Sbjct: 3 LLDSEGELVRNGGTYYLLPDRWALGGGIEAAATGTETCPLTVVRSPNEVSVGEPLRISSQ 62 Query: 294 FKILDITXGLILSLSFTYVTPCASTP 371 + I ++ + F CA +P Sbjct: 63 LRSGFIPDYSVVRIGFANPPKCAPSP 88
>gi|417198|sp|P32877|IT1B_PSOTE TRYPSIN INHIBITOR 1B (WTI-1B) >gi|68825|pir||TIWDKB trypsin inhibitor 1B (Kunitz) - winged bean >gi|351621|prf||0910196A inhibitor WTI,trypsin [Psophocarpus tetragonolobus] Length = 172 Frame 3 hits (HSPs): __________________________ Annotated Domains: __________________________________________________ __________________________________________________ Database sequence: | | | | | 172 0 50 100 150 __________________ Annotated Domains: BLOCKS BL00283A: Soybean trypsin inhibitor (Kun 2..22 BLOCKS BL00283B: Soybean trypsin inhibitor (Kun 40..54 BLOCKS BL00283C: Soybean trypsin inhibitor (Kun 126..134 BLOCKS BL00283D: Soybean trypsin inhibitor (Kun 139..148 DOMO DM00338: SOYBEANTRYPSININHIBITOR(KUNITZ) 1..168 Entrez active site: REACTIVE BOND (TRYPSIN). 64..65 PFAM Kunitz_legume: Trypsin and protease inhi 2..168 PRINTS KUNITZINHBTR1: Kunitz-type STI motif I - 2..31 PRINTS KUNITZINHBTR2: Kunitz-type STI motif II 40..60 PRINTS KUNITZINHBTR3: Kunitz-type STI motif III 115..134 PRINTS KUNITZINHBTR4: Kunitz-type STI motif IV 137..166 PRODOM PD000891: IDE3(3) IAAS(3) IT1B(2) 1..132 PRODOM PD009415: IDE3(3) IT1B(2) IT1A(1) 134..171 PROSITE SOYBEAN_KUNITZ: Soybean trypsin inhibito 3..19 __________________ Plus Strand HSPs: Score = 190 (66.9 bits), Expect = 5.4e-14, P = 5.4e-14 Identities = 43/86 (50%), Positives = 52/86 (60%), Frame = +3 Query: 114 VFDXEGNPIRNGGTYYVLPVIRGKGGGIXFAKTETETCPLTVVQSPFEVSKGLPLIISSP 293 + D EG +RNGGTYY+LP GGGI A T TETCPLTVV+SP EVS G PL ISS Sbjct: 3 LLDSEGELVRNGGTYYLLPDRWALGGGIEAAATGTETCPLTVVRSPNEVSVGEPLRISSQ 62 Query: 294 FKILDITXGLILSLSFTYVTPCASTP 371 + I ++ + F CA +P Sbjct: 63 LRSGFIPDYSLVRIGFANPPKCAPSP 88
>gi|625261|pir||A61433 trypsin inhibitor 2a (Kunitz) - winged bean Length = 180 Frame 3 hits (HSPs): _________________________ Annotated Domains: ______ _ __________________________________________________ Database sequence: | | | | | 180 0 50 100 150 __________________ Annotated Domains: Entrez modified site: pyrrolidone carboxylic ac 1 Entrez inhibit site: Arg (trypsin) 64 PROSITE SOYBEAN_KUNITZ: Soybean trypsin inhibito 3..19 __________________ Plus Strand HSPs: Score = 189 (66.5 bits), Expect = 7.0e-14, P = 7.0e-14 Identities = 39/86 (45%), Positives = 53/86 (61%), Frame = +3 Query: 114 VFDXEGNPIRNGGTYYVLPVIRGKGGGIXFAKTETETCPLTVVQSPFEVSKGLPLIISSP 293 + D EG +RNGGTYY++P +R GGGI AK E CPLTVV+S E S G P++I+SP Sbjct: 3 LLDVEGKAVRNGGTYYLVPQLRPHGGGIEVAKIGKEDCPLTVVKSLDENSNGEPIMIASP 62 Query: 294 FKILDITXGLILSLSFTYVTPCASTP 371 + I +L + F+ CA +P Sbjct: 63 LRSAFIPEYSLLKIGFSDPPKCAPSP 88
>gi|9665064|gb|AAF97266.1|AC034106_9 (AC034106) Contains similarity to a tumor-related protein from Nicotiana tabacum gb|U66263 and contains a trypsin and protease inhibitor PF|00197 domain. ESTs gb|AV561824, gb|T44961, gb|H36186, gb|T45060, gb|N38006, gb|F19847 come from this gene. [Ara> >gi|12083240|gb|AAG48779.1|AF332416_1 (AF332416) putative trypsin and protease inhibitor protein [Arabidopsis thaliana] Length = 196 Frame 3 hits (HSPs): __________________________ __________________________________________________ Database sequence: | | | | | 196 0 50 100 150 Plus Strand HSPs: Score = 181 (63.7 bits), Expect = 4.9e-13, P = 4.9e-13 Identities = 40/101 (39%), Positives = 59/101 (58%), Frame = +3 Query: 51 LALFLLCALTSSYQ---PSATADIVFDXEGNPIRNGGTYYVLPVIRGKGGGIXFAKTETE 221 L +FLL A+ S++ A + V D G + G YY+LPVIRG+GGG+ + +TE Sbjct: 5 LYIFLLLAVFISHRGVTTEAAVEPVKDINGKSLLTGVNYYILPVIRGRGGGLTMSNLKTE 64 Query: 222 TCPLTVVQSPFEVSKGLPLIISSPFKILDITXGLILSLSFT 344 TCP +V+Q FEVS+GLP+ S K I +++ F+ Sbjct: 65 TCPTSVIQDQFEVSQGLPVKFSPYDKSRTIPVSTDVNIKFS 105
>gi|124940|sp|P25700|IT2_PSOTE TRYPSIN INHIBITOR 2 (WTI-2) >gi|100088|pir||A37211 trypsin inhibitor 2 (Kunitz) - winged bean Length = 182 Frame 3 hits (HSPs): _______________________ Annotated Domains: _________________________________________________ __________________________________________________ Database sequence: | | | | | 182 0 50 100 150 __________________ Annotated Domains: DOMO DM00338: SOYBEANTRYPSININHIBITOR(KUNITZ) 1..176 Entrez active site: REACTIVE BOND (TRYPSIN). 64..65 PFAM Kunitz_legume: Trypsin and protease inhi 2..176 PRINTS KUNITZINHBTR1: Kunitz-type STI motif I - 2..31 PRINTS KUNITZINHBTR2: Kunitz-type STI motif II 40..60 PRINTS KUNITZINHBTR3: Kunitz-type STI motif III 118..137 PRINTS KUNITZINHBTR4: Kunitz-type STI motif IV 145..174 PRODOM PD000891: IDE3(3) IAAS(3) IT1B(2) 2..141 PRODOM PD009415: IDE3(3) IT1B(2) IT1A(1) 147..179 PROSITE SOYBEAN_KUNITZ: Soybean trypsin inhibito 3..19 __________________ Plus Strand HSPs: Score = 171 (60.2 bits), Expect = 5.6e-12, P = 5.6e-12 Identities = 37/84 (44%), Positives = 50/84 (59%), Frame = +3 Query: 120 DXEGNPIRNGGTYYVLPVIRGKGGGIXFAKTETETCPLTVVQSPFEVSKGLPLIISSPFK 299 D EG +RNGGTYY++P +R GGG+ AK E CPLTVV+S E S G P+ I+S + Sbjct: 5 DVEGKTVRNGGTYYLVPQLRPGGGGMEAAKVGNEDCPLTVVKSLDENSNGEPIRIASRLR 64 Query: 300 ILDITXGLILSLSFTYVTPCASTP 371 I +++L F CA +P Sbjct: 65 STFIPEYSLVNLGFADPPKCAPSP 88
>gi|2129874|pir||S65830 alpha fucosidase precursor - garden pea Length = 217 Frame 3 hits (HSPs): ________________________ Annotated Domains: ____ __________________________________________________ Database sequence: | | | | | | 217 0 50 100 150 200 __________________ Annotated Domains: PROSITE SOYBEAN_KUNITZ: Soybean trypsin inhibito 32..48 __________________ Plus Strand HSPs: Score = 169 (59.5 bits), Expect = 9.1e-12, P = 9.1e-12 Identities = 42/101 (41%), Positives = 58/101 (57%), Frame = +3 Query: 30 KMKSTTSLALFLLCALTSSYQPSATADI--VFDXEGNPIRNGGTYYVLPVIRGK-GGGIX 200 K S +L+ FL +T+ + D+ V D GNPI GG YY+LP IRG GGG+ Sbjct: 2 KPLSPLTLSFFLFVFITNLSLAFSNEDVEQVLDINGNPIFPGGQYYILPAIRGPPGGGVR 61 Query: 201 FAKTETETCPLTVVQSPFEVSKGLPLIISSPFKILDITXGLILS 332 +T TCP+TV+Q EV GLP+ F I +I+ G+I + Sbjct: 62 LGRTGDLTCPVTVLQDRQEVKYGLPV----KFVIPEISPGIIFT 101
>gi|81982|pir||JQ0949 proteinase inhibitor nodulin precursor - winged bean (fragment) >gi|257812|gb|AAB23733.1| (S46970) Kunitz protease inhibitor [Psophocarpus tetragonolobus] Length = 201 Frame 3 hits (HSPs): ________________ Annotated Domains: __________ __________________________________________________ Database sequence: | | | | || 201 0 50 100 150 200 __________________ Annotated Domains: Entrez domain: signal sequence (fragment) 1..20 PROSITE SOYBEAN_KUNITZ: Soybean trypsin inhibito 23..39 __________________ Plus Strand HSPs: Score = 159 (56.0 bits), Expect = 1.0e-10, P = 1.0e-10 Identities = 37/64 (57%), Positives = 43/64 (67%), Frame = +3 Query: 57 LFLLCALTSSYQPSATADI-VFDXEGNPIRNGGTYYVLPVIRGKGGGIXFAKTETETCPL 233 LFLL + TS Y PSATA V+D GN +RN G YY++PV G G GI T TE CPL Sbjct: 4 LFLLSSFTS-YLPSATAQQPVYDTNGNQLRNNGEYYIVPV-SG-GAGIDVVATGTEKCPL 60 Query: 234 TVVQS 248 T+VQS Sbjct: 61 TIVQS 65
>gi|7438249|pir||T03803 tumor-related protein, clone NF34 - common tobacco >gi|1762933|gb|AAC49969.1| (U66263) tumor-related protein [Nicotiana tabacum] Length = 210 Frame 3 hits (HSPs): ____________________________ __________________________________________________ Database sequence: | | | | | | 210 0 50 100 150 200 Plus Strand HSPs: Score = 159 (56.0 bits), Expect = 1.0e-10, P = 1.0e-10 Identities = 41/117 (35%), Positives = 63/117 (53%), Frame = +3 Query: 33 MKSTTSLALFLLCALTSSYQPSATADI---VFDXEGNPIRNGGTYYVLPVIRGKGGGIXF 203 MK+ FL+ ++ + S++A+ V D G +R G YY+LPV+RG+GGG+ Sbjct: 1 MKTNQLFLPFLIFTISFNSFLSSSAEAPPAVVDIAGKKLRTGIDYYILPVVRGRGGGLTL 60 Query: 204 AKTETETCPL-TVVQSPFEVSKGLPLIIS--SPFKILDITXGLILSLSFTYVTPCAST 368 T E+CPL VVQ E+ GLPL + +P K + I L++ F+ + C T Sbjct: 61 DSTGNESCPLDAVVQEQQEIKNGLPLTFTPVNPKKGV-IRESTDLNIKFSAASICVQT 117
>gi|3650368|emb|CAA09607.1| (AJ011398) profucosidase [Pisum sativum] Length = 217 Frame 3 hits (HSPs): __________________________ __________________________________________________ Database sequence: | | | | | | 217 0 50 100 150 200 Plus Strand HSPs: Score = 159 (56.0 bits), Expect = 1.0e-10, P = 1.0e-10 Identities = 42/111 (37%), Positives = 57/111 (51%), Frame = +3 Query: 42 TTSLALFLLCALTSSYQPSATADIVFDXEGNPIRNGGTYYVLPVIRGK-GGGIXFAKTET 218 T S LF+ S + + V D G PI GG YY+LP IRG GGG+ +T Sbjct: 8 TLSFLLFVFITTLSLAFSNEDVEQVLDVNGKPIFPGGQYYILPAIRGPPGGGVRLGRTGD 67 Query: 219 ETCPLTVVQSPFEVSKGLPLIISSPFKILDITXGLI-----LSLSFTYVTPCAST 368 TCP+TV+Q EV GLP+ F I I+ G+I + + +T CA + Sbjct: 68 LTCPVTVLQDRREVKNGLPV----KFVIPGISPGIIFTGTPIEIEYTKKPNCAKS 118
>gi|3859063|emb|CAA57931.1| (X82595) alpha-fucosidase [Pisum sativum] Length = 217 Frame 3 hits (HSPs): ___________________________ __________________________________________________ Database sequence: | | | | | | 217 0 50 100 150 200 Plus Strand HSPs: Score = 159 (56.0 bits), Expect = 1.0e-10, P = 1.0e-10 Identities = 43/117 (36%), Positives = 63/117 (53%), Frame = +3 Query: 30 KMKSTTSLALFLLCALTSSYQPSATADI--VFDXEGNPIRNGGTYYVLPVIRGK-GGGIX 200 K S +L+ FL +T+ + D+ V D GNPI G Y++LP IRG GGG+ Sbjct: 2 KPLSPLTLSFFLFVFITNLSLAFSNEDVEQVLDFNGNPIFPGVQYFILPAIRGPAGGGVR 61 Query: 201 FAKTETETCPLTVVQSPFEVSKGLPLIISSPFKILDITXGLI-----LSLSFTYVTPCAS 365 +T TCP+TV+Q EV GLP+ F I +I+ G+I + + +T CA Sbjct: 62 LGRTGDLTCPVTVLQDRQEVKNGLPV----KFVIPEISPGIIFTGTPIEIEYTKKPNCAK 117 Query: 366 T 368 + Sbjct: 118 S 118
>gi|1076518|pir||S49578 trypsin inhibitor homolog - garden pea Length = 217 Frame 3 hits (HSPs): ________________________ Annotated Domains: _________________________________________ __________________________________________________ Database sequence: | | | | | | 217 0 50 100 150 200 __________________ Annotated Domains: DOMO DM00338: SOYBEANTRYPSININHIBITOR(KUNITZ) 28..204 PROSITE SOYBEAN_KUNITZ: Soybean trypsin inhibito 32..48 __________________ Plus Strand HSPs: Score = 156 (54.9 bits), Expect = 2.2e-10, P = 2.2e-10 Identities = 40/101 (39%), Positives = 57/101 (56%), Frame = +3 Query: 30 KMKSTTSLALFLLCALTSSYQPSATADI--VFDXEGNPIRNGGTYYVLPVIRGK-GGGIX 200 K S +L+ FL +T+ + D+ V D GNPI G Y++LP IRG GGG+ Sbjct: 2 KPLSPLTLSFFLFVFITNLSLAFSNEDVEQVLDINGNPIFPGVQYFILPAIRGPPGGGVR 61 Query: 201 FAKTETETCPLTVVQSPFEVSKGLPLIISSPFKILDITXGLILS 332 +T TCP+TV+Q EV GLP+ F I +I+ G+I + Sbjct: 62 LGRTGDLTCPVTVLQDRQEVKYGLPV----KFVIPEISPGIIFT 101
>gi|5689162|dbj|BAA82840.1| (AB023648) miraculin homologue [Youngia japonica] Length = 142 Frame 3 hits (HSPs): ______________________________ __________________________________________________ Database sequence: | | | | 142 0 50 100 Plus Strand HSPs: Score = 153 (53.9 bits), Expect = 4.5e-10, P = 4.5e-10 Identities = 36/86 (41%), Positives = 50/86 (58%), Frame = +3 Query: 138 IRNGGTYYVLPVIRGKGGGIXFAKTETETCPLTVVQSPFEVSKGLPLIIS--SPFKILDI 311 +R+G YY+LPV RG GGG+ A T +TCPL VVQ+ EV GLPL + P K + I Sbjct: 1 LRSGTEYYILPVFRGMGGGLTLASTRNDTCPLDVVQADLEVDNGLPLTFTPVDPKKGV-I 59 Query: 312 TXGLILSLSFTYVTPCA-STPSSVERY 389 L++ F+ + C S ++E Y Sbjct: 60 RESTDLNIIFSASSICIQSNVWNLEEY 86
>gi|7438248|pir||T07871 miraculin homolog, root-knot nematode-induced - tomato >gi|2654440|gb|AAC63057.1| (U70076) Lemir [Lycopersicon esculentum] Length = 205 Frame 3 hits (HSPs): ___________________________ __________________________________________________ Database sequence: | | | | | | 205 0 50 100 150 200 Plus Strand HSPs: Score = 142 (50.0 bits), Expect = 6.6e-09, P = 6.6e-09 Identities = 38/108 (35%), Positives = 59/108 (54%), Frame = +3 Query: 60 FLLCALTSSYQPSATADI---VFDXEGNPIRNGGTYYVLPVIRGKGGGIXFAKTETETCP 230 FL+ A++ + S+ A+ V D +G +R G YY+LPV+RG+GGG+ + CP Sbjct: 10 FLILAISFNSLLSSAAESPPEVVDIDGKILRTGVDYYILPVVRGRGGGLTMDSIGDKMCP 69 Query: 231 L-TVVQSPFEVSKGLPLIIS--SPFKILDITXGLILSLSFTYVTPCAST 368 L VVQ E+ +GLPL + P K + I L++ F+ + C T Sbjct: 70 LDAVVQEHNEIDQGLPLTFTPVDPKKGV-IRESTDLNIIFSANSICVQT 117
>gi|12324312|gb|AAG52121.1|AC010556_3 (AC010556) putative trypsin inhibitor; 19671-20297 [Arabidopsis thaliana] Length = 208 Frame 3 hits (HSPs): __________________ __________________________________________________ Database sequence: | | | | | | 208 0 50 100 150 200 Plus Strand HSPs: Score = 141 (49.6 bits), Expect = 8.5e-09, P = 8.5e-09 Identities = 31/77 (40%), Positives = 48/77 (62%), Frame = +3 Query: 60 FLLCALTSSYQPSATADIVFDXEGNPIRNGGTYYVLPVIRGKGGGIXFAKTETETCPLTV 239 +L+ ALT+ +A +V D +GN + + +YYVLPVIRG+GGG+ A + CP + Sbjct: 6 YLVLALTAVLASNAYGAVV-DIDGNAMFHE-SYYVLPVIRGRGGGLTLAGRGGQPCPYDI 63 Query: 240 VQSPFEVSKGLPLIISS 290 VQ EV +G+P+ S+ Sbjct: 64 VQESSEVDEGIPVKFSN 80
>gi|6166552|sp|P13087|MIRA_RICDU MIRACULIN PRECURSOR (MIR) >gi|1109652|dbj|BAA07603.1| (D38598) miraculin precursor [Richadella dulcifica] Length = 220 Frame 3 hits (HSPs): _______________________________ Annotated Domains: _________________________________________________ __________________________________________________ Database sequence: | | | | | | 220 0 50 100 150 200 __________________ Annotated Domains: BLOCKS BL00283A: Soybean trypsin inhibitor (Kun 6..26 BLOCKS BL00283B: Soybean trypsin inhibitor (Kun 47..61 BLOCKS BL00283C: Soybean trypsin inhibitor (Kun 141..149 BLOCKS BL00283D: Soybean trypsin inhibitor (Kun 159..168 DOMO DM00338: SOYBEANTRYPSININHIBITOR(KUNITZ) 3..187 Entrez glycosylation site 71 Entrez glycosylation site 215 PFAM Kunitz_legume: Trypsin and protease inhi 35..216 PRINTS KUNITZINHBTR1: Kunitz-type STI motif I - 6..35 PRINTS KUNITZINHBTR2: Kunitz-type STI motif II 47..67 PRINTS KUNITZINHBTR3: Kunitz-type STI motif III 131..150 PRINTS KUNITZINHBTR4: Kunitz-type STI motif IV 157..186 PRODOM PD202523: MIRA_RICDU 1..33 PRODOM PD000891: IDE3(3) IAAS(3) IT1B(2) 35..177 PRODOM PD016101: MIRA(1) O48625(1) P93378(1) 179..215 PROSITE SOYBEAN_KUNITZ: Soybean trypsin inhibito 36..52 __________________ Plus Strand HSPs: Score = 138 (48.6 bits), Expect = 1.8e-08, P = 1.8e-08 Identities = 43/133 (32%), Positives = 63/133 (47%), Frame = +3 Query: 33 MKSTTSLAL---FLLCALTSSYQPSATA-----DIVFDXEGNPIRNGGTYYVLPVIRGKG 188 MK T L+L F+ L ++ P +A + V D +G +R G YY++PV+R G Sbjct: 1 MKELTMLSLSFFFVSALLAAAANPLLSAADSAPNPVLDIDGEKLRTGTNYYIVPVLRDHG 60 Query: 189 GGIXFAKTE---TETCPLTVVQSPFEVSKGLPLIISSPFKILDITX-GLILSLSFTYVTP 356 GG+ + T T CP VVQ+ EV PL D+ L+++F+ P Sbjct: 61 GGLTVSATTPNGTFVCPPRVVQTRKEVDHDRPLAFFPENPKEDVVRVSTDLNINFSAFMP 120 Query: 357 CASTPSSVERYXK 395 C T S+V R K Sbjct: 121 CRWTSSTVWRLDK 133
>gi|543616|pir||JX0310 kunitz type subtilisin inhibitor, CLSI-II - Canavalia lineata Length = 190 Frame 3 hits (HSPs): __________________ Annotated Domains: _______________________________________________ __________________________________________________ Database sequence: | | | | | 190 0 50 100 150 __________________ Annotated Domains: DOMO DM00338: SOYBEANTRYPSININHIBITOR(KUNITZ) 2..178 Entrez inhibit site: Arg (subtilisin) 68 Entrez inhibit site: Gly (subtilisin) 69 __________________ Plus Strand HSPs: Score = 137 (48.2 bits), Expect = 2.3e-08, P = 2.3e-08 Identities = 28/63 (44%), Positives = 36/63 (57%), Frame = +3 Query: 108 DIVFDXEGNPIRNGGTYYVLPVIRGK-GGGIXFAKTETETCPLTVVQSPFEVSKGLPLII 284 D+V D PI GG YY++P I G GGG+ AKT CP+TV+Q EV G P+ Sbjct: 4 DVVMDASSKPIFPGGEYYIMPAIWGPPGGGVRLAKTRNSDCPVTVLQDYGEVIFGQPVKF 63 Query: 285 SSP 293 + P Sbjct: 64 TLP 66
>gi|543617|pir||JX0311 kunitz type subtilisin inhibitor, CLSI-III - Canavalia lineata Length = 183 Frame 3 hits (HSPs): __________________ Annotated Domains: _ __________________________________________________ Database sequence: | | | | | 183 0 50 100 150 __________________ Annotated Domains: Entrez inhibit site: Arg (subtilisin) 68 Entrez inhibit site: Gly (subtilisin) 69 __________________ Plus Strand HSPs: Score = 137 (48.2 bits), Expect = 2.3e-08, P = 2.3e-08 Identities = 28/63 (44%), Positives = 36/63 (57%), Frame = +3 Query: 108 DIVFDXEGNPIRNGGTYYVLPVIRGK-GGGIXFAKTETETCPLTVVQSPFEVSKGLPLII 284 D+V D PI GG YY++P I G GGG+ AKT CP+TV+Q EV G P+ Sbjct: 4 DVVMDASSKPIFPGGEYYIMPAIWGPPGGGVRLAKTRNSDCPVTVLQDYGEVIFGQPVKF 63 Query: 285 SSP 293 + P Sbjct: 64 TLP 66
>gi|5689168|dbj|BAA82843.1| (AB023651) miraculin homologue [Solanum melongena] Length = 160 Frame 3 hits (HSPs): __________________________ __________________________________________________ Database sequence: | | | | | 160 0 50 100 150 Plus Strand HSPs: Score = 136 (47.9 bits), Expect = 2.9e-08, P = 2.9e-08 Identities = 34/82 (41%), Positives = 46/82 (56%), Frame = +3 Query: 129 GNPIRNGGTYYVLPVIRGKGGGIXFAKTETETCPL-TVVQSPFEVSKGLPLIISSPF--K 299 G +R G YY+LPV+RG+GGG+ +TCPL VVQ EV +GLPL + PF K Sbjct: 1 GKILRTGIDYYILPVVRGRGGGLTMDSIGNKTCPLDAVVQEQEEVKQGLPLTFT-PFNPK 59 Query: 300 ILDITXGLILSLSFTYVTPCAST 368 I L++ F+ + C T Sbjct: 60 KGVIRESTDLNIIFSANSICVQT 82 WARNING: HSPs involving 61 database sequences were not reported due to the limiting value of parameter B = 50. Parameters: filter=none matrix=BLOSUM62 V=50 B=50 E=10 gi H=1 sort_by_pvalue echofilter ctxfactor=5.93 Query ----- As Used ----- ----- Computed ---- Frame MatID Matrix name Lambda K H Lambda K H Std. 0 BLOSUM62 0.318 0.135 0.401 +3 0 BLOSUM62 0.318 0.135 0.401 0.322 0.139 0.413 Q=9,R=2 0.244 0.0300 0.180 n/a n/a n/a +2 0 BLOSUM62 0.318 0.135 0.401 0.341 0.149 0.509 Q=9,R=2 0.244 0.0300 0.180 n/a n/a n/a +1 0 BLOSUM62 0.318 0.135 0.401 0.346 0.153 0.541 Q=9,R=2 0.244 0.0300 0.180 n/a n/a n/a -1 0 BLOSUM62 0.318 0.135 0.401 0.337 0.151 0.469 Q=9,R=2 0.244 0.0300 0.180 n/a n/a n/a -2 0 BLOSUM62 0.318 0.135 0.401 0.367 0.166 0.668 Q=9,R=2 0.244 0.0300 0.180 n/a n/a n/a -3 0 BLOSUM62 0.318 0.135 0.401 0.355 0.160 0.581 Q=9,R=2 0.244 0.0300 0.180 n/a n/a n/a Query Frame MatID Length Eff.Length E S W T X E2 S2 +3 0 138 134 10. 73 3 12 22 0.11 33 30 0.098 36 +2 0 139 136 10. 73 3 12 22 0.11 33 30 0.10 36 +1 0 139 137 10. 73 3 12 22 0.11 33 30 0.10 36 -1 0 139 135 10. 73 3 12 22 0.11 33 30 0.099 36 -2 0 139 135 10. 73 3 12 22 0.11 33 30 0.099 36 -3 0 138 135 10. 73 3 12 22 0.11 33 30 0.099 36 Statistics: Database: /usr/local/dot5/sl_home/beauty/seqdb/blast/nr Title: nr Release date: unknown Posted date: 4:06 PM CST Feb 28, 2001 Format: BLAST # of letters in database: 197,782,623 # of sequences in database: 625,274 # of database sequences satisfying E: 111 No. of states in DFA: 588 (58 KB) Total size of DFA: 166 KB (192 KB) Time to generate neighborhood: 0.01u 0.00s 0.01t Elapsed: 00:00:01 No. of threads or processors used: 6 Search cpu time: 137.49u 1.24s 138.73t Elapsed: 00:00:23 Total cpu time: 137.53u 1.28s 138.81t Elapsed: 00:00:24 Start: Mon Oct 1 22:43:07 2001 End: Mon Oct 1 22:43:31 2001 WARNINGS ISSUED: 2
Annotated Domains Database: March 14, 2000
Release Date: March 14, 2000