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DOI 10.1016/j.bbrc.2006.07.156
Title Effects of fibulin-5 on attachment, adhesion, and proliferation of primary human endothelial cells
Creator/Author Preis, M. [Department of Cardiovascular Medicine, Lady Davis Carmel Medical Center, 7 Michal Street, Haifa 34632 (Israel)]|[Bruce Rappaport Faculty of Medicine, Technion IIT, Haifa (Israel)] ; Cohen, T. ; Sarnatzki, Y. [Department of Cardiovascular Medicine, Lady Davis Carmel Medical Center, 7 Michal Street, Haifa 34632 (Israel)]|[MultiGene Vascular Systems (MGVS) Ltd., Technion IIT, Haifa (Israel)] ; Ben Yosef, Y. [Department of Cardiovascular Medicine, Lady Davis Carmel Medical Center, 7 Michal Street, Haifa 34632 (Israel)]|[MultiGene Vascular Systems (MGVS) Ltd., Technion IIT, Haifa (Israel)] ; Schneiderman, J. [MultiGene Vascular Systems (MGVS) Ltd., Technion IIT, Haifa (Israel)]|[Vascular Surgery, Sheeba Medical Center, Tel Hasomer, Technion IIT, Haifa (Israel)] ; Gluzman, Z. [Department of Cardiovascular Medicine, Lady Davis Carmel Medical Center, 7 Michal Street, Haifa 34632 (Israel)]|[MultiGene Vascular Systems (MGVS) Ltd., Technion IIT, Haifa (Israel)] ; Koren, B. [Department of Cardiovascular Medicine, Lady Davis Carmel Medical Center, 7 Michal Street, Haifa 34632 (Israel)]|[MultiGene Vascular Systems (MGVS) Ltd., Technion IIT, Haifa (Israel)] ; Lewis, B.S. [Department of Cardiovascular Medicine, Lady Davis Carmel Medical Center, 7 Michal Street, Haifa 34632 (Israel)]|[MultiGene Vascular Systems (MGVS) Ltd., Technion IIT, Haifa (Israel)]|[Bruce Rappaport Faculty of Medicine, Technion IIT, Haifa (Israel)] ; Shaul, Y. [MultiGene Vascular Systems (MGVS) Ltd., Technion IIT, Haifa (Israel)]|[Department of Molecular Genetics, Weizmann Institute of Science, Rehovot, Technion IIT, Haifa (Israel)] ; Flugelman, M.Y. [Department of Cardiovascular Medicine, Lady Davis Carmel Medical Center, 7 Michal Street, Haifa 34632 (Israel)]|[MultiGene Vascular Systems (MGVS) Ltd., Technion IIT, Haifa (Israel)]|[Bruce Rappaport Faculty of Medicine, Technion IIT, Haifa (Israel)]. E-mail: myf@tx.technion.ac.il
Publication Date2006 Sep 29
OSTI IdentifierOSTI ID: 20854490
Other Number(s)Journal ID: ISSN 0006-291X; BBRCA9; TRN: US07R0265027480
Resource TypeJournal Article
Resource RelationJournal: Biochemical and Biophysical Research Communications; Journal Volume: 348; Journal Issue: 3; Other Information: DOI: 10.1016/j.bbrc.2006.07.156; PII: S0006-291X(06)01719-0; Copyright (c) 2006 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved; Country of input: International Atomic Energy Agency (IAEA)
Subject60 APPLIED LIFE SCIENCES; BIOLOGICAL STRESS; CELL PROLIFERATION; ENZYME IMMUNOASSAY; PEPTIDES; PHENOTYPE; PROSTHESES; VEINS
Description/Abstract Background: Fibulin-5 is a novel extracellular protein that is thought to act as a bridging peptide between elastin fibers and cell surface integrins in blood vessel wall. Fibulin-5 binding to endothelial cell (EC) surface integrins may effect cell proliferation and cell attachment to extracellular matrix (ECM) or to artificial surfaces. In this paper, we describe the effects of fibulin-5 on attachment, adhesion, and proliferation of primary human EC. After demonstrating that fibulin-5 over-expression inhibited EC proliferation, we tested the hypothesis that co-expression of fibulin-5 and VEGF{sub 165} will lead to unique EC phenotype that will exhibit increased adherence properties and retain its proliferation capacity. Methods and results: Fibulin-5 and VEGF{sub 165} gene transfer to primary human saphenous vein endothelial cells was accomplished using retroviral vectors encoding the two genes. Transgene expression was verified using immunohistochemistry, Western blotting, and ELISA. Fibulin 5 over-expression tended to improve immediate EC attachment (30 min after seeding) and improved significantly adhesion (>40%) under shear stress tested 24 h after EC seeding. The effects of fibulin-5 and VEGF{sub 165} on EC proliferation in the presence or absence of basic FGF were also tested. EC expressing fibulin-5 had reduced proliferation while VEGF{sub 165} co-expression ameliorated this effect. Conclusion: Fibulin-5 improved EC attachment to artificial surfaces. Dual transfer of fibulin-5 and VEGF{sub 165} resulted in EC phenotype with increased adhesion and improved proliferation. This unique EC phenotype can be useful for tissue engineering on endovascular prostheses.
Country of PublicationUnited States
LanguageEnglish
FormatSize: page(s) 1024-1033
System Entry Date2007 Apr 23

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