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Library:898 (dbEST ID)
Gessler Wilms tumor
Library Description
| Organism: | Homo sapiens |
| Sex: | pooled (6) |
| Vector: | pSPORT1 |
| Vector type: | plasmid (ampicillin resistant) |
| Cloning site 1: | SalI |
| Cloning site 2: | NotI |
| Lab host: | DH10B |
RNA was prepared from a pool of 6 anonymous Wilms' tumor RNAs. RNA was prepared by acid-phenol, followed by one round of oligo dT selection. cDNA library preparation was with the BRL/Life Tech. Superscript Plasmid system. An oligo-dT NotI primer for first strand synthesis generated gcggccgccc(t)n at the 3' end of the clones. A 5' SalI adaptor was used with sequence 5'-gtcgacccacgcgtccg-3'. Resulting cDNAs were size selected (average size 2 kb), NotI digested, and ligated into NotI/SalI-cut pSPORT1. Library was constructed by Dr. Manfred Gessler.
Source: Wilson RK, Washington University School of Medicine
Gene Content Analysis
4,855 ESTs from this library were grouped into 2,892 UniGene entries (putative genes) [UniGene build #217, 04-Dec-2008]. EST counts for each entry may be used to calculate an approximate expression level in transcripts per million (TPM).
| ESTs | TPM | UniGene Entry | ||
|---|---|---|---|---|
| 1 | 206 |  |
Hs.13366 | Small nuclear ribonucleoprotein 48kDa (U11/U12) (SNRNP48), mRNA. |
| 1 | 206 | |
Hs.133397 | Integrin alpha 6B [human, mRNA Partial, 528 nt]. |
| 1 | 206 | |
Hs.133089 | DNA fragmentation factor, 40kDa, beta polypeptide (caspase-activated DNase), mRNA (cDNA clone IMAGE:4820856). |
| 1 | 206 | |
Hs.133029 | CDNA FLJ30785 fis, clone FEBRA2000901. |
| 1 | 206 | |
Hs.133009 | CDNA clone IMAGE:4329532. |
| 1 | 206 | |
Hs.132902 | Adenylyl cyclase-associated protein homolog CAP2 (CAP2). |
| 1 | 206 | |
Hs.132868 | NY-REN-60 antigen. |
| 1 | 206 | |
Hs.132591 | Solute carrier family 10 (sodium/bile acid cotransporter family), member 4 (SLC10A4), mRNA. |
| 1 | 206 | |
Hs.132483 | CDNA: FLJ22365 fis, clone HRC06613. |
| 1 | 206 | |
Hs.132370 | Cleavage stimulation factor. |
| 1 | 206 | |
Hs.132225 | Phosphoinositide 3-kinase p85 subunit splicing variant p46 mRNA, complete cds, alternatively spliced. |
| 1 | 206 | |
Hs.132161 | CDNA FLJ60701 complete cds, highly similar to WD repeat domain phosphoinositide-interacting protein 3. |
| 1 | 206 | |
Hs.131887 | DnaJ (Hsp40) homolog, subfamily C, member 21, mRNA (cDNA clone IMAGE:4307151). |
| 1 | 206 | |
Hs.131269 | Retinoic acid receptor responder (tazarotene induced) 1, mRNA (cDNA clone MGC:24933 IMAGE:4776506). |
| 1 | 206 | |
Hs.130866 | CDNA FLJ30885 fis, clone FEBRA2004987. |
| 1 | 206 | |
Hs.130746 | Basic-leucine zipper nuclear factor (JEM-1). |
| 1 | 206 | |
Hs.130692 | CDNA PSEC0021 fis, clone NT2RP1000279. |
| 1 | 206 | |
Hs.130307 | Transcribed locus. |
| 1 | 206 | |
Hs.130031 | Homo sapiens, Similar to triple functional domain (PTPRF interacting), clone IMAGE:5497679. |
| 1 | 206 | |
Hs.128959 | PCF11p homolog. |
| 1 | 206 | |
Hs.128866 | ATPase type 13A2 (ATP13A2), transcript variant 2, mRNA. |
| 1 | 206 | |
Hs.12851 | Phosphatidylserine synthase 2 (PTDSS2), mRNA. |
| 1 | 206 | |
Hs.128258 | Ecto-NOX disulfide-thiol exchanger 1 (ENOX1), transcript variant 2, mRNA. |
| 1 | 206 | |
Hs.127950 | CDNA FLJ51158 complete cds, highly similar to Bromodomain-containing protein 1. |
| 1 | 206 | |
Hs.127361 | CDNA FLJ60829 complete cds. |
| 1 | 206 | |
Hs.12707 | DnaJ (Hsp40) homolog, subfamily C, member 13, mRNA (cDNA clone IMAGE:5107059). |
| 1 | 206 | |
Hs.127022 | HPTP epsilon mRNA for protein tyrosine phosphatase epsilon. |
| 1 | 206 | |
Hs.1270 | Glucokinase (GCK). |
| 1 | 206 | |
Hs.126941 | Family with sequence similarity 49, member B, mRNA (cDNA clone MGC:29507 IMAGE:5017493). |
| 1 | 206 | |
Hs.126550 | Vacuolar protein sorting 4 homolog B (S. cerevisiae), mRNA (cDNA clone MGC:48692 IMAGE:6042862). |
| 1 | 206 | |
Hs.126497 | AE binding protein 2 (AEBP2), transcript variant 1, mRNA. |
| 1 | 206 | |
Hs.126248 | CDNA clone IMAGE:4661943. |
| 1 | 206 | |
Hs.125474 | Leupaxin, mRNA (cDNA clone IMAGE:5271433). |
| 1 | 206 | |
Hs.124696 | 3-hydroxybutyrate dehydrogenase, type 2, mRNA (cDNA clone MGC:32990 IMAGE:4838679). |
| 1 | 206 | |
Hs.124503 | Zinc finger homeodomain protein. |
| 1 | 206 | |
Hs.124246 | CDNA FLJ36756 fis, clone UTERU2018200. |
| 1 | 206 | |
Hs.124027 | CDNA FLJ57394 complete cds, highly similar to Selenide, water dikinase 1 (EC 2.7.9.3). |
| 1 | 206 | |
Hs.12393 | TDP-glucose 4,6-dehydratase, mRNA (cDNA clone IMAGE:3510977). |
| 1 | 206 | |
Hs.123198 | Myosin. |
| 1 | 206 | |
Hs.123119 | Mother against dpp (Mad) related protein. |
| 1 | 206 | |
Hs.123070 | HOX4D mRNA for a homeobox protein. |
| 1 | 206 | |
Hs.122186 | Nephrin (NPHS1). |
| 1 | 206 | |
Hs.121870 | KIAA0607 protein. |
| 1 | 206 | |
Hs.121260 | CDNA FLJ51483 complete cds, highly similar to Multiple inositol polyphosphate phosphatase 1 precursor (EC 3.1.3.62). |
| 1 | 206 | |
Hs.120165 | CDNA FLJ60432 complete cds, highly similar to Leucine zipper-EF-hand-containing transmembrane protein 1. |
| 1 | 206 | |
Hs.119882 | MRNA PLSTIRE for serine/threonine protein kinase. |
| 1 | 206 | |
Hs.119192 | Histone H2A.Z, 5'UTR region. |
| 1 | 206 | |
Hs.119177 | ADP-ribosylation factor 3, mRNA (cDNA clone MGC:33515 IMAGE:4817874). |
| 1 | 206 | |
Hs.118640 | Dishevelled 2 (DVL2). |
| 1 | 206 | |
Hs.118631 | Homo sapiens, Similar to timeless homolog (Drosophila), clone IMAGE:6063535. |
| 1 | 206 | |
Hs.118076 | CDNA FLJ40595 fis, clone THYMU2010705, highly similar to 40S RIBOSOMAL PROTEIN S4, X ISOFORM. |
| 1 | 206 | |
Hs.117929 | Hypothetical LOC401397, mRNA (cDNA clone IMAGE:4244115). |
| 1 | 206 | |
Hs.11776 | U4/U6-associated RNA splicing factor (PRP3). |
| 1 | 206 | |
Hs.117545 | CAMP-specific phosphodiesterase PDE4D2 (PDE4D). |
| 1 | 206 | |
Hs.117183 | Transcribed locus, strongly similar to XP_529375.2 PREDICTED: hypothetical protein [Pan troglodytes]. |
| 1 | 206 | |
Hs.117060 | Extracellular matrix protein 2, female organ and adipocyte specific, mRNA (cDNA clone IMAGE:5725929). |
| 1 | 206 | |
Hs.116935 | Early hematopoietic zinc finger (EHZF gene). |
| 1 | 206 | |
Hs.116602 | Methionyl-tRNA synthetase 2, mitochondrial, mRNA (cDNA clone IMAGE:4999520). |
| 1 | 206 | |
Hs.116531 | Transcribed locus. |
| 1 | 206 | |
Hs.116471 | Cadherin-11. |
| 1 | 206 | |
Hs.116424 | Transcribed locus. |
| 1 | 206 | |
Hs.114111 | Limkain beta 2. |
| 1 | 206 | |
Hs.114033 | SSR alpha subunit. |
| 1 | 206 | |
Hs.113823 | CDNA FLJ75855 complete cds, highly similar to Homo sapiens ClpX caseinolytic peptidase X homolog (CLPX), mRNA. |
| 1 | 206 | |
Hs.113684 | Cadherin-12. |
| 1 | 206 | |
Hs.11355 | Thymopoietin gamma. |
| 1 | 206 | |
Hs.113314 | CDNA FLJ39735 fis, clone SMINT2016286. |
| 1 | 206 | |
Hs.113157 | Integrin alpha subunit. |
| 1 | 206 | |
Hs.11314 | Chromosome 20 open reading frame 4, mRNA (cDNA clone MGC:4208 IMAGE:2905315). |
| 1 | 206 | |
Hs.112499 | Homo sapiens, clone IMAGE:5191797. |
| 1 | 206 | |
Hs.111801 | CDNA FLJ60199 complete cds, highly similar to Arsenite-resistance protein 2. |
| 1 | 206 | |
Hs.1116 | CDNA FLJ38099 fis, clone D3OST1000238, highly similar to Tumor necrosis factor receptor superfamily member 3 precursor. |
| 1 | 206 | |
Hs.111577 | CDNA PSEC0047 fis, clone NT2RP1001042, highly similar to Integral membrane protein 2C. |
| 1 | 206 | |
Hs.11108 | Cdk5 and Abl enzyme substrate 1 (CABLES1), transcript variant 1, mRNA. |
| 1 | 206 | |
Hs.110637 | Homeobox A10 (HOXA10), transcript variant 2, mRNA. |
| 1 | 206 | |
Hs.109 | Dipeptidase 1 (renal) (DPEP1), transcript variant 2, mRNA. |
| 1 | 206 | |
Hs.10848 | BMS1 homolog, ribosome assembly protein (yeast), mRNA (cDNA clone MGC:166808 IMAGE:9007274). |
| 1 | 206 | |
Hs.108301 | Nuclear receptor subfamily 2, group C, member 1, mRNA (cDNA clone MGC:48851 IMAGE:5183723). |
| 1 | 206 | |
Hs.108112 | Lipopolysaccharide specific response-71 protein, mRNA sequence. |
| 1 | 206 | |
Hs.108106 | CDNA FLJ60383 complete cds, highly similar to Ubiquitin-like PHD and RING fingerdomain-containing protein 1 (EC 6.3.2.-). |
| 1 | 206 | |
Hs.108080 | Cysteine-rich peptide. |
| 1 | 206 | |
Hs.106909 | Full length insert cDNA clone ZB85E09. |
| 1 | 206 | |
Hs.106876 | ATPase, H+ transporting, lysosomal 38kDa, V0 subunit d1 (ATP6V0D1), mRNA. |
| 1 | 206 | |
Hs.106674 | BRCA1 associated protein 1 (BAP1). |
| 1 | 206 | |
Hs.10649 | ICB1 delta variant (ICB1) mRNA, 3' UTR. |
| 1 | 206 | |
Hs.105791 | CDNA clone IMAGE:6186815. |
| 1 | 206 | |
Hs.105584 | Mitogen- and stress-activated protein kinase-2 (MSK2). |
| 1 | 206 | |
Hs.105460 | DKFZP564O0823 protein, mRNA (cDNA clone MGC:3551 IMAGE:3028327). |
| 1 | 206 | |
Hs.105105 | CDNA FLJ23804 fis, clone HEP22939. |
| 1 | 206 | |
Hs.104744 | Mex-3 homolog B (C. elegans), mRNA (cDNA clone MGC:39639 IMAGE:5266259). |
| 1 | 206 | |
Hs.102798 | Proteasome subunit C2. |
| 1 | 206 | |
Hs.101364 | Asparagine synthetase domain containing 1, mRNA (cDNA clone MGC:4948 IMAGE:3453136). |
Source: NCBI UniGene
EST Sequences
6,198 EST sequences from this library have been submitted to the public sequence database as of 04-Dec-2008.
Source: NCBI GenBank
Click the button to save sequences to a file (FASTA format)
Keywords
- kidney
- kidney tumor
- unknown developmental stage
- non-normalized
Source: NCBI Clone Registry