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Title Fluorescent probe studies of normal, persistently infected, Rous sarcoma virus-transformed, and trypsinized rat cells
Creator/Author Burleson, G.R. ; Kulpa, C.F. ; Edwards, H.E. ; Thomas, J.K.
Publication Date1978 Jan 01
OSTI IdentifierOSTI ID: 6000949
Other Number(s)CODEN: ECREA
Resource TypeJournal Article
Resource RelationExp. Cell Res. ; Vol/Issue: 116
Research OrgUniv. of Notre Dame, IN
Subject550700 -- Microbiology ;550700 -- Microbiology ;550200 -- Biochemistry; ;ANIMAL CELLS-- FLUORESCENCE;CELL MEMBRANES-- RESPONSE MODIFYING FACTORS; BUTYRIC ACID;INFECTIOUS DISEASES;LIFETIME;LIPIDS;NAPHTHOLS;ONCOGENIC VIRUSES;PYRENE;RATS;SCINTILLATION QUENCHING;TRYPSIN;VIRUSES
Related SubjectANIMALS;AROMATICS;CARBOXYLIC ACIDS;CELL CONSTITUENTS;CONDENSED AROMATICS;DISEASES;ENZYMES;HYDROCARBONS;HYDROLASES;HYDROXY COMPOUNDS;LUMINESCENCE;MAMMALS;MEMBRANES;MICROORGANISMS;MONOCARBOXYLIC ACIDS;ORGANIC ACIDS;ORGANIC COMPOUNDS;PARASITES;PEPTIDE HYDROLASES;PHENOLS;RODENTS;VERTEBRATES;VIRUSES
Description/Abstract The fluorescent probes pyrene, pyrene butyric acid and N-phenyl 1-naphthylamine were used to study membranes of normal cells, RSV-transformed cells, cells treated with a proteolytic enzyme, and cells persistently infected with lymphocytic choriomeningitis virus.^The lifetimes of excited pyrene and pyrene butyric acid showed only minor changes when these probes were in normal, transformed, trypsinized or persistently infected cells.^However, pyrene, but not pyrene butyric acid, lifetimes are shorter in cell membranes than in homogeneous solvents.^The quenching of excited pyrene in cells by quencher molecules was slower than corresponding reactions in homogeneous solutions indicating that the probe was screened from the quenchers by the membrane.^However, quenching reactions with the pyrene butyric acid probe were similar in cells and homogeneous solvents.^This indicates that pyrene and pyrene butyric acid reside in different lipid regions of the membrane.^Transformed and trypsinized cells showed increased membrane fluidity compared to normal and persistently infected cells.^Membrane fluidity was determined from the excimer/monomer fluorescence ratios of pyrene, and by the polarization of N-phenyl 1-naphthylamine fluorescence.^Several techniques distinguished between normal and transformed or trypsinized cells; however, the only parameter unique to viral transformation was a blue shift of the fluorescence maxima of N-phenly 1-naphthylamine.^This shift reflected a less polar environment for N-phenyl 1-naphthylamine in virus-transformed cells.
Country of PublicationUnited States
LanguageEnglish
FormatPages: 291-300
System Entry Date2001 May 13

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