In Vivo Assay for the Synthesis of Hydroxyproline-rich Proteins

Plant Physiol. 1980 December; 66(6): 1044–1047.

PMCID: PMC440786

In Vivo Assay for the Synthesis of Hydroxyproline-rich Proteins ¹

Joseph E. Varner and Jane E. Burton

Department of Biology, Washington University, St. Louis, Missouri 63130

¹ This work was supported by National Science Foundation Grant PCM 76-14178.

Abstract

Simple methods are described for following in vivo the rate of peptidylproline hydroxylation and for determining what proportion of the total proline incorporated into protein is hydroxylated.

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Selected References

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Chrispeels, Maarten J. Synthesis and Secretion of Hydroxyproline Containing Macromolecules in Carrots. I. Kinetic Analysis. Plant Physiol. 1969 Aug;44(8):1187–1193. [PubMed]
Sadava, D; Chrispeels, MJ. Hydroxyproline biosynthesis in plant cells. Peptidyl proline hydroxylase from carrot disks. Biochim Biophys Acta. 1971 Feb 10;227(2):278–287. [PubMed]
Mitra, R; Gross, RD; Varner, JE. An intact assay for enzymes that labilize C--H bonds. Anal Biochem. 1975 Mar;64(1):102–109. [PubMed]
STONE, N; MEISTER, A. Function of ascorbic acid in the conversion of proline to collagen hydroxyproline. Nature. 1962 May 12;194:555–557. [PubMed]