	US 7,364,886 B2
	Chemical sensor enhanced by direct coupling of redox enzyme to conductive surface
Melissa S. Hasenbank, Seattle, Wash. (US); Elain S. Fu, Seattle, Wash. (US); and Paul Yager, Seattle, Wash. (US)
Assigned to University of Washington, Seattle, Wash. (US)
Filed on Feb. 28, 2006, as Appl. No. 11/364,436.
Prior Publication US 2007/0202559 A1, Aug. 30, 2007
Int. Cl. C12N 11/00 (2006.01); C12Q 1/26 (2006.01)

U.S. Cl. 435—174 [435/25]

15 Claims

1. A system for detecting electrochemical oxidoreduction activity mediated by a redox enzyme at a site remote from the enzyme comprising:

(a) a continuous conductive surface having a first region and at least one additional region, wherein a redox enzyme is immobilized on the first region of the conductive surface, wherein the additional region is electrically coupled via the continuous conductive surface with the first region, and the redox enzyme is not present in the additional region; and

(b) means for detecting oxidation or reduction of a substrate capable of producing a detectable signal upon oxidation or reduction at the additional region, wherein the substrate is located at the additional region.