|
| Status |
Public on Apr 17, 2008 |
| Title |
B1-WV |
| Sample type |
RNA |
| |
|
| Source Name |
Pre-interferon liver biopsy
|
| Organism(s) |
Homo sapiens |
| Characteristics |
Pre-interferon
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 microg total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Target RNA is reverse transcribed into cDNA and in-vitro transcription is performed to generate biotin-labeled cRNA for subsequent hybridization. Hybridized target cRNA is stained with streptavidin phycoerythrin and arrays are scanned using a GeneArray Scanner at an excitation wavelength of 488nm. Light emissions at 570nm are proportional to the bound target at each oligonucleotides position on the GeneChip® array.
|
| Scan protocol |
GeneChips were scanned using a GeneArray Scanner at an excitation wavelength of 488nm. Light emissions at 570nm are proportional to the bound target at each oligonucleotides position on the GeneChip® array.
|
| Description |
Pre-interferon liver biopsy
|
| Data processing |
GCRMA-calculated Signal intensity
|
| |
|
| Submission date |
Apr 16, 2008 |
| Contact name |
Edward James Oakeley |
| E-mail(s) |
edward.oakeley@fmi.ch
|
| Organization name |
Friedrich Miescher Institute for Biomedical Research
|
| Street address |
Maulbeerstrasse 66
|
| City |
Basel |
| ZIP/Postal code |
4058 |
| Country |
Switzerland |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE11190 |
Interferon signaling and treatment outcome in chronic hepatitis C |
|
