Fish Image
U.S. Food & Drug Administration
Seafood Products Research Center
Center for Food Safety & Applied Nutrition
Regulatory Fish Encyclopedia:
IEF Gel Lane Banding Pattern Peak Report Data
Gel Image

Peak Report Data from Isoelectric Focusing for a specimen of:
Blacktip Shark  ----  Go to Display Gel Lane Navigator.


FDA Seafood List Scientific Name: Carcharhinus limbatus
FDA Seafood List Common Name: Blacktip Shark
FDA Seafood List Market Name: Shark
Unique IEF Gel Code: 19950809gel15
Unique Web Code: btg01Srr
Tissue Aliquot Code: carclimb I94-001 #02 Gel Analysis Conditions
Gel ImageFillet ImageCalibrated Gel Image
Gel ImageFish ImageCalibrated Gel

DISPLAY GEL LANE NAVIGATOR
RETURN TO TOP OF THIS DOCUMENT
Lane a
pI Markers
Lane b
Specimen
Lane c
pI Markers
Lane d
Co-Migrated
Lane e
Specimen
Lane f
pI Markers
Go to Gel Schematic

 
DISPLAY GEL LANE LETTER: Lane a
DIGITAL REPORT GEL LANE: 6
LANE TYPE: pI Markers
ACTUAL GEL LANE: 8
Go to Gel Schematic
Return to Lane Navigator
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
13.5652.90.15889184.951568.56.33.567.5180.155588
24.5536115.90.35002473.961836.85.14.55360.66.70.333619
35.252723.20.44140775.420569.36.95.2527.67.20.423382
45.8583536.80.64072266.6651611.48.55.85835.65.90.640602
56.55118552.30.46868486.132210.57.96.551184.27.70.446633
66.85126355.70.263563114.773238.26.16.851263.311.10.243407
77.35144863.90.296518126.5206111.28.47.351447.112.10.303674
88.15177078.10.0906789162.45754.23.18.151769.715.60.0888135
98.45188583.10.155313123.177585.44.18.451885.711.80.151739
108.65195886.40.17268103.043855.23.98.651957.510.20.168547
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
119.3220197.10.4921361411.299119.314.59.32197.614.40.443454
PeakCounter: 11
DISPLAY GEL LANE LETTER: Lane b
DIGITAL REPORT GEL LANE: 7
LANE TYPE: Specimen
ACTUAL GEL LANE: 9
Go to Gel Schematic
Return to Lane Navigator
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
14.89744919.80.018513760.1583740.30.34.897449.15.80.0153238
25.03648521.40.023433770.2495180.50.45.036484.47.10.0199035
35.13651122.50.021206380.2477690.50.45.136511.27.70.0181067
45.25155224.30.028193850.2326020.50.45.251552.44.70.0276886
55.34159526.20.0267502100.3990250.80.75.341595.99.60.0235452
65.72377834.30.037899280.51086610.95.723776.97.80.036803
75.86684737.30.024926860.2349440.50.45.866848.75.70.0234229
85.97690039.70.0479253110.8467391.71.55.976900.511.10.0431933
96.083952420.067162460.6780181.41.26.083951.85.90.0645215
106.1498043.20.016310550.159650.30.36.14980.75.30.0170308
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
116.2100944.50.143227163.893967.86.86.21006.916.30.135087
126.254103545.60.0597718151.353052.72.46.2541036.515.10.0505975
136.3651089480.035363440.1523330.30.36.3651086.83.60.0235595
146.431112149.40.5015391513.27326.523.36.4311125.814.70.509653
157.054133358.80.0268026110.47794510.87.0541329.210.80.0249722
167.14136560.20.0203167130.444340.90.87.141364.513.30.0187807
177.374145364.10.0182682100.3717830.70.77.3741458.110.40.0201403
187.589153967.90.0189835130.366650.70.67.5891540.713.50.0153606
198.258180479.50.132139245.6727411.3108.2581809.824.20.132497
208.635194785.80.188268.501631714.98.6351950.325.60.187729
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
219.064210592.80.2765442711.857923.720.89.0642106.226.60.25168
PeakCounter: 21
DISPLAY GEL LANE LETTER: Lane c
DIGITAL REPORT GEL LANE: 8
LANE TYPE: pI Markers
ACTUAL GEL LANE: 10
Go to Gel Schematic
Return to Lane Navigator
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
13.5823.60.134371307.0603711.49.53.584.9300.132782
24.55363160.32473773.7262654.55364.16.90.305888
35.253423.60.34205284.425657.15.95.2533.67.60.328322
45.8584937.50.53417676.82438119.25.85849.770.546718
56.55117952.10.3574384.70197.66.36.5511797.90.336923
66.85125755.50.227578114.06916.65.56.851258.510.80.213171
77.35144563.80.344018106.114839.98.27.351442.810.40.330438
88.15176177.70.0909044172.65564.33.68.151759.617.20.0869487
98.45187782.90.115084122.4822343.38.451876.612.10.115771
108.65195386.20.151609102.5080443.48.651952.49.80.144887
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
119.3218996.60.4999992817.465428.223.59.32179.227.90.353125
PeakCounter: 11
DISPLAY GEL LANE LETTER: Lane d
DIGITAL REPORT GEL LANE: 9
LANE TYPE: Co-Migrated
ACTUAL GEL LANE: 11
Go to Gel Schematic
Return to Lane Navigator
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
13.51004.40.125025276.255816.963.5103.226.90.131024
24.55409180.24052393.580383.93.54.55408.89.10.221404
34.82948221.30.01370890.1974080.20.24.829479.88.60.0129994
44.94851322.60.040670280.5452510.60.54.948513.87.70.0400475
55.07545240.0580166222.447112.72.45.07555.121.50.0641671
65.257925.50.235356103.739134.13.65.2578.810.10.208013
75.28561727.20.032779110.563830.60.55.285621.810.60.030023
85.45469330.60.016903290.2424640.30.25.454695.38.90.015406
95.53272832.10.0196406100.4194870.50.45.532727.710.40.02267
105.59675733.40.010241490.1723670.20.25.596760.990.0108596
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
115.68579735.20.082610691.235041.41.25.685796.98.70.0799722
125.8587138.40.45737175.4872265.35.85872.46.60.466413
135.96792340.70.0314578120.5898080.60.65.967923.912.10.0274307
146.083975430.040385660.5222060.60.56.083972.45.50.0531059
156.178101744.90.068949870.6824640.70.76.1781017.47.20.053489
166.209103145.50.13567681.393381.51.36.2091031.88.20.0961608
176.263105546.50.0302949301.581881.71.56.2631043.4300.02975
186.384110948.90.02688770.2694540.30.36.3841111.87.10.0214764
196.478115150.80.3953231510.329911.3106.4781152.314.50.401448
206.55118352.20.23455893.312543.63.26.551181.38.90.209367
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
216.85126155.60.228196103.6499443.56.851261.29.70.213136
226.897127856.40.0610660.5950170.70.66.8971279.76.50.0517278
237.35144163.60.314501105.471165.37.351440.510.20.303424
247.771160570.80.0229076120.4300780.50.47.7711603.112.20.0199133
258.15175377.30.0782537212.7319132.68.151753.9210.0732578
268.278180479.60.158113184.790145.34.68.2781803.917.80.152121
278.45187382.60.0711452121.499511.61.48.451874.612.10.0700931
288.65195786.30.249134239.499910.49.28.651960.923.40.228898
299.0652109930.2502773010.43411.410.19.0652121.3300.196229
309.3219596.80.426979138.489129.38.29.32192.813.10.366821
PeakCounter: 30
DISPLAY GEL LANE LETTER: Lane e
DIGITAL REPORT GEL LANE: 10
LANE TYPE: Specimen
ACTUAL GEL LANE: 12
Go to Gel Schematic
Return to Lane Navigator
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
15.10653523.60.0075996590.1072260.20.25.1065399.50.00637902
25.22857225.20.019429490.2671950.50.55.228573.48.50.0177305
35.32461727.20.017904290.2421240.50.45.324616.98.90.0153844
45.71980135.30.024891590.52425410.95.719796.69.20.0320868
55.86186738.20.020923370.2569820.50.45.861868.97.40.019497
65.97391940.50.0400101100.6130111.215.973920.69.70.035608
76.08297042.80.053177650.4942110.86.0829705.40.0514821
86.188101944.90.11822153.482136.85.96.1881023.215.20.129586
96.228103845.80.074298770.3462230.70.66.2281041.57.20.0271612
106.368110348.60.032023140.144960.30.26.3681102.13.60.0225905
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
116.454114350.40.4985871614.590728.324.76.4541146.515.70.525293
127.065134259.20.026006290.3693370.70.67.0651340.69.30.0225137
137.406146964.80.0298805120.5525091.10.97.4061469.311.80.0265235
147.612155068.30.011572180.2158360.40.47.6121553.88.40.0144212
157.698158469.80.00882418120.2607630.50.47.6981589.811.60.0127321
168.276181179.90.120267224.624897.88.2761812.622.50.116172
178.641195386.10.2311792811.111221.618.88.6411955.4280.224096
189.075211593.30.2955882813.356125.922.69.0752115.427.70.272359
PeakCounter: 18
DISPLAY GEL LANE LETTER: Lane f
DIGITAL REPORT GEL LANE: 11
LANE TYPE: pI Markers
ACTUAL GEL LANE: 13
Go to Gel Schematic
Return to Lane Navigator
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
13.59140.134489307.1482512.293.593.1300.134435
24.5537016.30.28953483.712346.44.74.55369.37.60.276764
35.253323.50.32280784.547537.85.75.2532.58.10.315958
45.8584737.40.60138377.2136512.49.15.85847.36.60.617861
56.55118652.40.13231674.50917.75.76.551178.96.50.388694
66.85125955.60.261273114.759998.266.851258.9110.2441
77.35144763.90.345066116.6047611.38.37.351444.811.40.325761
88.151765780.087764172.503974.33.28.151765.316.70.0846492
98.451879830.141171122.975785.13.88.451878.5120.140115
108.65195086.10.159869112.959845.13.78.651949.910.60.158214
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
119.32197970.4895711511.469119.614.59.32194.214.90.434777
PeakCounter: 11
Gel Banding Patterns Display in HTML using GIF stacking technique
(Uses Migration Position Normalized Data from above text peak reports)

Return to Lane Navigator    Go to Gel Schematic Key

a b c d e f
MK SP MK CO SP MK
white spacer for gel schematic
pI 3.50 label for gel schematic
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pI 4.55 label for gel schematic
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pI 5.20 label for gel schematic
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pI 3.50 label for gel schematic
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pI 4.55 label for gel schematic
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pI 5.20 label for gel schematic
white spacer for gel schematic
pI 5.8 label for gel schematic
white spacer for gel schematic
pI 6.55 label for gel schematic
white spacer for gel schematic
pI 6.85 label for gel schematic
white spacer for gel schematic
pI 7.35 label for gel schematic
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pI 8.15 label for gel schematic
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pI 8.45 label for gel schematic
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Key to Gel Schematic: MK=pI Marker Lanes, pI bands are in red
SP=Specimen Lanes, Specimen bands are in blue
CO=Co-migrated Lanes of pI Markers and Specimen (pI bands are in red, Specimen bands are in blue)
Note:  In co-migrated lanes, some pI Markers and Specimen bands may overlap in experiment.  Red pI
band is reported when overlapped peak position is equal to nominal pI Marker position.  Blue Specimen
band is reported when overlapped peak position deviates slightly from nominal pI Marker position.
Weak (<2% rel. intensity), Medium (2-6% rel. intensity), and Strong (>6% rel. intensity) band intensities indicated by gel schematic band thickness.
Return to Top of Gel Schematic

PeakCount: 102
HTML Forms TextArea Normalized Peak-Position Peak-Area for Copy-Paste events
DISPLAY GEL LANE LETTER: Lane a
DIGITAL REPORT GEL LANE: 6
LANE TYPE: pI Markers
ACTUAL GEL LANE: 8
PeakCount: 11 Return to Lane Navigator
DISPLAY GEL LANE LETTER: Lane b
DIGITAL REPORT GEL LANE: 7
LANE TYPE: Specimen
ACTUAL GEL LANE: 9
PeakCount: 21 Return to Lane Navigator
DISPLAY GEL LANE LETTER: Lane c
DIGITAL REPORT GEL LANE: 8
LANE TYPE: pI Markers
ACTUAL GEL LANE: 10
PeakCount: 11 Return to Lane Navigator
DISPLAY GEL LANE LETTER: Lane d
DIGITAL REPORT GEL LANE: 9
LANE TYPE: Co-Migrated
ACTUAL GEL LANE: 11
PeakCount: 30 Return to Lane Navigator
DISPLAY GEL LANE LETTER: Lane e
DIGITAL REPORT GEL LANE: 10
LANE TYPE: Specimen
ACTUAL GEL LANE: 12
PeakCount: 18 Return to Lane Navigator
DISPLAY GEL LANE LETTER: Lane f
DIGITAL REPORT GEL LANE: 11
LANE TYPE: pI Markers
ACTUAL GEL LANE: 13
PeakCount: 11 Return to Lane Navigator



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EXPERIMENTAL CONDITIONS: Gels were scanned at 600 d.p.i. in Adobe Photoshop 3.02 using a Sharp JX-325 Scanner with Film Scanner Attachment. Gels were analyzed for Densitometry, Intregration, and Migration Position Normalization in EXCEL 5.0 Format. *.xls files were imported into Paradox 7.0 to save as *.db files.

 RETURN TO TOP THIS DOCUMENT
Delphi 4.0 Generation of this Isoelectric Focusing (IEF) RFE PAGE 5 Tools in HTML
This HTML is automatically generated from a series of Paradox 7.0 database filesof IEF peak reports is controlled with a main RFEIEF Paradox 7.0 database withDelphi 3.0 front end (see Delphi 2.0 form) and back end for facile addition and modification of the Content of these RFE PAGE 5 HTML tools.
Contact Brad Tenge at btenge@ora.fda.gov for details.


RFE Contacts
A summary list of RFE contact personnel is available at http://www.cfsan.fda.gov/~frf/rfecon.html.

For isoelectric focusing laboratory experiments for fish species identification, you may contact

For isoelectric focusing gel analysis and pattern recognition applications, you may contact

For CFSAN web consultation and posting, you may contact


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Last updated on 2003-FEB-27 by bjt/frf/nln