LOCUS NP_417367 365 aa linear BCT 27-JAN-2009
DEFINITION peptide chain release factor RF-2 [Escherichia coli str. K-12
substr. MG1655].
ACCESSION NP_417367
VERSION NP_417367.1 GI:16130793
DBSOURCE REFSEQ: accession NC_000913.2
KEYWORDS .
SOURCE Escherichia coli str. K-12 substr. MG1655
ORGANISM Escherichia coli str. K-12 substr. MG1655
Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
Enterobacteriaceae; Escherichia.
REFERENCE 1 (residues 1 to 365)
AUTHORS Riley,M., Abe,T., Arnaud,M.B., Berlyn,M.K., Blattner,F.R.,
Chaudhuri,R.R., Glasner,J.D., Horiuchi,T., Keseler,I.M., Kosuge,T.,
Mori,H., Perna,N.T., Plunkett,G. III, Rudd,K.E., Serres,M.H.,
Thomas,G.H., Thomson,N.R., Wishart,D. and Wanner,B.L.
TITLE Escherichia coli K-12: a cooperatively developed annotation
snapshot--2005
JOURNAL Nucleic Acids Res. 34 (1), 1-9 (2006)
PUBMED 16397293
REMARK Publication Status: Online-Only
REFERENCE 2 (residues 1 to 365)
AUTHORS Blattner,F.R., Plunkett,G., Bloch,C.A., Perna,N.T., Burland,V.,
Riley,M., Collado-Vides,J., Glasner,J.D., Rode,C.K., Mayhew,G.F.,
Gregor,J., Davis,N.W., Kirkpatrick,H.A., Goeden,M.A., Rose,D.J.,
Mau,B. and Shao,Y.
TITLE The complete genome sequence of Escherichia coli K-12
JOURNAL Science 277 (5331), 1453-1474 (1997)
PUBMED 9278503
REFERENCE 3 (sites)
AUTHORS Kang,W.K., Icho,T., Isono,S., Kitakawa,M. and Isono,K.
TITLE Characterization of the gene rimK responsible for the addition of
glutamic acid residues to the C-terminus of ribosomal protein S6 in
Escherichia coli K12
JOURNAL Mol. Gen. Genet. 217 (2-3), 281-288 (1989)
PUBMED 2570347
REFERENCE 4 (residues 1 to 365)
AUTHORS Arnaud,M., Berlyn,M.K.B., Blattner,F.R., Galperin,M.Y.,
Glasner,J.D., Horiuchi,T., Kosuge,T., Mori,H., Perna,N.T.,
Plunkett,G. III, Riley,M., Rudd,K.E., Serres,M.H., Thomas,G.H. and
Wanner,B.L.
TITLE Workshop on Annotation of Escherichia coli K-12
JOURNAL Unpublished
REMARK Woods Hole, Mass., on 14-18 November 2003 (sequence corrections)
REFERENCE 5 (residues 1 to 365)
AUTHORS Glasner,J.D., Perna,N.T., Plunkett,G. III, Anderson,B.D.,
Bockhorst,J., Hu,J.C., Riley,M., Rudd,K.E. and Serres,M.H.
TITLE ASAP: Escherichia coli K-12 strain MG1655 version m56
JOURNAL Unpublished
REMARK ASAP download 10 June 2004 (annotation updates)
REFERENCE 6 (residues 1 to 365)
AUTHORS Hayashi,K., Morooka,N., Mori,H. and Horiuchi,T.
TITLE A more accurate sequence comparison between genomes of Escherichia
coli K12 W3110 and MG1655 strains
JOURNAL Unpublished
REMARK GenBank accessions AG613214 to AG613378 (sequence corrections)
REFERENCE 7 (residues 1 to 365)
AUTHORS Perna,N.T.
TITLE Escherichia coli K-12 MG1655 yqiK-rfaE intergenic region, genomic
sequence correction
JOURNAL Unpublished
REMARK GenBank accession AY605712 (sequence corrections)
REFERENCE 8 (residues 1 to 365)
AUTHORS Rudd,K.E.
TITLE A manual approach to accurate translation start site annotation: an
E. coli K-12 case study
JOURNAL Unpublished
REFERENCE 9 (residues 1 to 365)
CONSRTM NCBI Genome Project
TITLE Direct Submission
JOURNAL Submitted (27-JAN-2009) National Center for Biotechnology
Information, NIH, Bethesda, MD 20894, USA
REFERENCE 10 (residues 1 to 365)
AUTHORS Rudd,K.E.
TITLE Direct Submission
JOURNAL Submitted (22-DEC-2008) Department of Biochemistry and Molecular
Biology, University of Miami Miller School of Medicine, 118 Gautier
Bldg., Miami, FL 33136, USA
REMARK Annotation update from ecogene.org as a multi-database
collaboration
REFERENCE 11 (residues 1 to 365)
AUTHORS Rudd,K.E.
TITLE Direct Submission
JOURNAL Submitted (12-MAR-2008) Department of Biochemistry and Molecular
Biology, University of Miami Miller School of Medicine, 118 Gautier
Bldg., Miami, FL 33136, USA
REMARK Annotation update from ecogene.org as a multi-database
collaboration
REFERENCE 12 (residues 1 to 365)
AUTHORS Rudd,K.E.
TITLE Direct Submission
JOURNAL Submitted (26-SEP-2007) Department of Biochemistry and Molecular
Biology, University of Miami Miller School of Medicine, 118 Gautier
Bldg., Miami, FL 33136, USA
REMARK Annotation update from ecogene.org as a multi-database
collaboration
REFERENCE 13 (residues 1 to 365)
AUTHORS Rudd,K.E.
TITLE Direct Submission
JOURNAL Submitted (24-APR-2007) Department of Biochemistry and Molecular
Biology, University of Miami Miller School of Medicine, 118 Gautier
Bldg., Miami, FL 33136, USA
REMARK Annotation update from ecogene.org as a multi-database
collaboration
REFERENCE 14 (residues 1 to 365)
AUTHORS Plunkett,G. III.
TITLE Direct Submission
JOURNAL Submitted (07-FEB-2006) Laboratory of Genetics, University of
Wisconsin, 425G Henry Mall, Madison, WI 53706-1580, USA
REMARK Protein updates by submitter
REFERENCE 15 (residues 1 to 365)
AUTHORS Plunkett,G. III.
TITLE Direct Submission
JOURNAL Submitted (10-JUN-2004) Laboratory of Genetics, University of
Wisconsin, 425G Henry Mall, Madison, WI 53706-1580, USA
REMARK Sequence update by submitter
REFERENCE 16 (residues 1 to 365)
AUTHORS Plunkett,G. III.
TITLE Direct Submission
JOURNAL Submitted (13-OCT-1998) Laboratory of Genetics, University of
Wisconsin, 425G Henry Mall, Madison, WI 53706-1580, USA
REFERENCE 17 (residues 1 to 365)
AUTHORS Blattner,F.R. and Plunkett,G. III.
TITLE Direct Submission
JOURNAL Submitted (02-SEP-1997) Laboratory of Genetics, University of
Wisconsin, 425G Henry Mall, Madison, WI 53706-1580, USA
REFERENCE 18 (residues 1 to 365)
AUTHORS Blattner,F.R. and Plunkett,G. III.
TITLE Direct Submission
JOURNAL Submitted (16-JAN-1997) Laboratory of Genetics, University of
Wisconsin, 425G Henry Mall, Madison, WI 53706-1580, USA
COMMENT PROVISIONAL REFSEQ: This record has not yet been subject to final
NCBI review. The reference sequence was derived from AAC75929.
Method: conceptual translation.
FEATURES Location/Qualifiers
source 1..365
/organism="Escherichia coli str. K-12 substr. MG1655"
/strain="K-12"
/sub_strain="MG1655"
/db_xref="taxon:511145"
Protein 1..365
/product="peptide chain release factor RF-2"
/function="factor; Proteins - translation and
modification"
/function="2.3.2 information transfer; protein related;
translation"
/function="7.1 location of gene products; cytoplasm"
/calculated_mol_wt=41120
Region 3..365
/region_name="prfB"
/note="peptide chain release factor 2; Validated;
PRK00578"
/db_xref="CDD:100609"
Region 95..195
/region_name="RF-1"
/note="RF-1 domain; cl02875"
/db_xref="CDD:121618"
Region 225..337
/region_name="RF-1"
/note="RF-1 domain; cl02875"
/db_xref="CDD:121618"
CDS 1..365
/gene="prfB"
/locus_tag="b2891"
/gene_synonym="ECK2886"
/gene_synonym="supK"
/gene_synonym="JW5847"
/coded_by="complement(join(NC_000913.2:3033206..3034228,
NC_000913.2:3034230..3034304))"
/experiment="N-terminus verified by Edman degradation:
PMID 3889910"
/ribosomal_slippage
/GO_component="GO:0005737 - cytoplasm"
/GO_process="GO:0006412 - translation"
/note="an in-frame premature UGA termination codon is
located within the prfB sequence, and a naturally occuring
+1 frameshift is required for synthesis of RF-2"
/transl_table=11
/db_xref="ASAP:ABE-0009488"
/db_xref="UniProtKB/Swiss-Prot:P07012"
/db_xref="ECOCYC:EG10762"
/db_xref="EcoGene:EG10762"
/db_xref="GeneID:947369"
ORIGIN
1 mfeinpvnnr iqdltersdv lrgyldydak kerleevnae leqpdvwnep eraqalgker
61 ssleavvdtl dqmkqgledv sgllelavea ddeetfneav aeldaleekl aqlefrrmfs
121 geydsadcyl diqagsggte aqdwasmler mylrwaesrg fkteiieese gevagiksvt
181 ikisgdyayg wlrtetgvhr lvrkspfdsg grrhtsfssa fvypevdddi dieinpadlr
241 idvyrtsgag gqhvnrtesa vrithiptgi vtqcqndrsq hknkdqamkq mkaklyelem
301 qkknaekqam ednksdigwg sqirsyvldd srikdlrtgv etrntqavld gsldqfieas
361 lkagl
//