Poster Presentation 1B-10
Assessment of Low Cost Supplementation Sources of
Soybean Waste on Lipase Production by Solid-State Fermentation
Gean D. L.
P. Vargas, Helen Treichel, Débora de Oliveira, Marco Di Luccio*
and Denise
M. G. Freire†
Department
of Food Engineering, URI – Campus de Erechim
Av. Sete
de Setembro 1621
Erechim,
99700-000, RS, Brazil
Phone: +55-54-520-9000
Fax: +55-54-520-9090
E-Mail: diluccio@uricer.edu.br
†Department
of Biochemistry – IQ/UFRJ
Centro de Tecnologia, Bl. A, Sala
549-2
Rio de Janeiro, 21945-900, RJ,
Brazil
Microbial lipases represent a large share of the enzyme market. Issues of production and final product costs
are of great relevance to the industry, particularly in applications where the
costs of the enzyme may make the process economically unfeasible. Thus, the use of agroindustry wastes, such as
soybean bran, as raw materials for lipase production could reduce the
production costs of lipases. In this
work we investigated the supplementation of soybean bran to maximize lipase
production by Penicillium simplicissimum.
Soybean oil, swine slaughterhouse
wastewater, yeast hydrolysate and corn steep liquor were added to the bran to
assess the effect of different kinds of supplementation on lipase yield. All the factors were evaluated using
experimental design and the conditions that optimize lipase production were
obtained using the response surface method. In all experiments, lipase, protease and water
activities, pH and moisture of the solid medium were determined after 48h of
incubation. The fermentation kinetics of the best operation condition was
followed. The results show that the
temperature associated to low cake moisture can increase lipase production. It was also observed that the soybean waste is
a sufficiently nutrient-rich substrate for the microorganism, since the
increase of carbon or nitrogen sources showed a negative effect on lipase
production. The most favorable conditions
for lipase production were determined through experiments carried out without
supplementation. The maximum lipase
activity obtained using the optimized conditions was 29.5 U/g dry solid medium.