Poster Presentation 1B-10

Assessment of Low Cost Supplementation Sources of Soybean Waste on Lipase Production by Solid-State Fermentation

Gean D. L. P. Vargas, Helen Treichel, Débora de Oliveira, Marco Di Luccio*

and Denise M. G. Freire†

Department of Food Engineering, URI – Campus de Erechim

Av. Sete de Setembro 1621

Erechim, 99700-000, RS, Brazil

Phone: +55-54-520-9000

Fax: +55-54-520-9090

E-Mail: diluccio@uricer.edu.br

†Department of Biochemistry – IQ/UFRJ

Centro de Tecnologia, Bl. A, Sala 549-2

Rio de Janeiro, 21945-900, RJ, Brazil

Microbial lipases represent a large share of the enzyme market. Issues of production and final product costs are of great relevance to the industry, particularly in applications where the costs of the enzyme may make the process economically unfeasible. Thus, the use of agroindustry wastes, such as soybean bran, as raw materials for lipase production could reduce the production costs of lipases. In this work we investigated the supplementation of soybean bran to maximize lipase production by Penicillium simplicissimum. Soybean oil, swine slaughterhouse wastewater, yeast hydrolysate and corn steep liquor were added to the bran to assess the effect of different kinds of supplementation on lipase yield. All the factors were evaluated using experimental design and the conditions that optimize lipase production were obtained using the response surface method. In all experiments, lipase, protease and water activities, pH and moisture of the solid medium were determined after 48h of incubation. The fermentation kinetics of the best operation condition was followed. The results show that the temperature associated to low cake moisture can increase lipase production. It was also observed that the soybean waste is a sufficiently nutrient-rich substrate for the microorganism, since the increase of carbon or nitrogen sources showed a negative effect on lipase production. The most favorable conditions for lipase production were determined through experiments carried out without supplementation. The maximum lipase activity obtained using the optimized conditions was 29.5 U/g dry solid medium.