RFE Fish Species Identification Isoelectric Focusing Tools

U.S. Food & Drug Administration
Seafood Products Research Center
Center for Food Safety & Applied Nutrition
Regulatory Fish Encyclopedia:
IEF Gel Lane Banding Pattern Peak Report Data

Peak Report Data from Isoelectric Focusing for a specimen of:
Oilfish(*) ---- Go to Display Gel Lane Navigator.

FDA Seafood List Scientific Name: Ruvettus(*) pretiosus(*)
FDA Seafood List Common Name: Oilfish(*)
FDA Seafood List Market Name: Oilfish(*)
Unique IEF Gel Code: 19970423gel04
Unique Web Code: oig04Srr
Tissue Aliquot Code: ruvepret H95-002 #06m Gel Analysis Conditions


Gel Image	Fish Image	Calibrated Gel

DISPLAY GEL LANE NAVIGATOR
RETURN TO TOP OF THIS DOCUMENT
Lane a pI Markers	Lane b Specimen	Lane c pI Markers	Lane d Co-Migrated	Lane e Specimen	Lane f pI Markers	Go to Gel Schematic

**DISPLAY GEL LANE LETTER:** Lane a
**DIGITAL REPORT GEL LANE:** 6
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 8
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	3.5	64	2.9	0.240247	21	8.54912	14.9	10.9	3.5	67	20.9	0.230511
2	4.55	382	17.1	0.203112	12	4.12878	7.2	5.3	4.55	382.2	11.9	0.195188
3	5.2	560	25.1	0.293549	11	5.48506	9.6	7	5.2	560.2	10.7	0.289784
4	5.85	870	39	0.464752	7	5.93615	10.3	7.6	5.85	870	7.1	0.469973
5	6.55	1209	54.1	0.28561	9	4.1804	7.3	5.3	6.55	1207.7	8.8	0.267563
6	6.85	1293	57.9	0.24575	9	3.84612	6.7	4.9	6.85	1292.2	9.4	0.230577
7	7.35	1473	66	0.301385	11	5.46226	9.5	7	7.35	1471.3	10.7	0.28895
8	8.15	1768	79.2	0.106655	18	3.37073	5.9	4.3	8.15	1768.8	18.2	0.10464
9	8.45	1888	84.5	0.188253	14	4.38986	7.6	5.6	8.45	1888.7	14.1	0.175435
10	8.65	1958	87.7	0.157798	11	2.9719	5.2	3.8	8.65	1958.2	11.2	0.149378
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	9.3	2197	98.4	0.429656	14	9.091	15.8	11.6	9.3	2192.1	14.2	0.360157

PeakCounter: 11

**DISPLAY GEL LANE LETTER:** Lane b
**DIGITAL REPORT GEL LANE:** 7
**LANE TYPE:** Specimen
**ACTUAL GEL LANE:** 9
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	3.541	73	3.3	0.0630043	7	0.754011	1.7	1.1	3.541	72.7	6.8	0.0621151
2	4.449	348	15.6	0.341026	7	4.60057	10.4	6.9	4.449	348.3	7.5	0.346247
3	5.615	757	34	0.0482809	7	0.563035	1.3	0.8	5.615	757	6.9	0.0460256
4	5.736	815	36.6	0.214221	9	3.01896	6.8	4.5	5.736	816.3	8.5	0.199941
5	5.884	886	39.8	0.59695	7	7.27922	16.5	10.9	5.884	887.4	6.9	0.594795
6	5.991	938	42.2	0.297365	10	4.38805	9.9	6.5	5.991	938.8	9.6	0.258759
7	6.08	981	44.1	0.143759	7	1.67183	3.8	2.5	6.08	981.1	7.3	0.129068
8	6.125	1003	45.1	0.0924334	13	1.90996	4.3	2.8	6.125	999	13	0.0826908
9	6.424	1148	51.6	0.0951023	9	1.38908	3.1	2.1	6.424	1147.5	9.4	0.0835111
10	6.449	1160	52.1	0.0906667	6	0.86287	2	1.3	6.449	1158.3	5.9	0.0822641
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	6.607	1225	55.1	0.129084	8	1.65566	3.8	2.5	6.607	1222	8.3	0.112172
12	6.686	1247	56	0.387582	4	2.28076	5.2	3.4	6.686	1246.8	3.6	0.360171
13	6.743	1263	56.8	0.189533	6	1.99766	4.5	3	6.743	1263.4	5.8	0.195323
14	6.8	1279	57.5	0.362906	6	3.69674	8.4	5.5	6.8	1281	6.3	0.332348
15	6.977	1339	60.2	0.294368	8	3.90544	8.8	5.8	6.977	1337.7	7.6	0.289792
16	7.137	1397	62.8	0.126797	11	2.04979	4.6	3.1	7.137	1396.7	11.1	0.104507
17	7.772	1629	73.2	0.0694666	17	2.10899	4.8	3.1	7.772	1623.9	17.4	0.0683279

PeakCounter: 17

**DISPLAY GEL LANE LETTER:** Lane c
**DIGITAL REPORT GEL LANE:** 8
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 10
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	3.5	63	2.8	0.289934	21	10.395	17.1	12.2	3.5	67.2	21.3	0.275858
2	4.55	381	17.2	0.267934	11	4.75408	7.8	5.6	4.55	382.6	10.8	0.249249
3	5.2	563	25.3	0.351615	9	5.82663	9.6	6.8	5.2	563.3	9.5	0.347555
4	5.85	875	39.4	0.487913	7	6.4765	10.6	7.6	5.85	876.7	7.5	0.490456
5	6.55	1215	54.7	0.283664	10	4.53372	7.4	5.3	6.55	1213.4	9.7	0.263437
6	6.85	1299	58.5	0.232388	10	3.94321	6.5	4.6	6.85	1298.5	10.4	0.214058
7	7.35	1481	66.7	0.288432	10	5.132	8.4	6	7.35	1478.3	10.4	0.279467
8	8.15	1773	79.8	0.124529	20	4.29845	7.1	5	8.15	1772.2	20.1	0.120881
9	8.45	1887	85	0.196973	14	4.4855	7.4	5.3	8.45	1886.5	13.7	0.184925
10	8.65	1955	88	0.173514	11	3.35967	5.5	3.9	8.65	1954.5	11.5	0.165123
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	9.3	2191	98.6	0.423086	12	7.73666	12.7	9.1	9.3	2187.1	11.6	0.377393

PeakCounter: 11

**DISPLAY GEL LANE LETTER:** Lane d
**DIGITAL REPORT GEL LANE:** 9
**LANE TYPE:** Co-Migrated
**ACTUAL GEL LANE:** 11
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	3.5	64	2.9	0.23971	19	8.37794	11.2	8.3	3.5	70.1	18.9	0.249809
2	4.412	355	16	0.274942	8	4.0337	5.4	4	4.412	354.6	8.2	0.276455
3	4.55	399	18	0.168438	17	4.62854	6.2	4.6	4.55	400.3	16.8	0.15553
4	5.2	585	26.3	0.1942	14	4.46499	5.9	4.4	5.2	584.3	13.7	0.18435
5	5.587	764	34.4	0.0382857	8	0.547167	0.7	0.5	5.587	766.5	8	0.0386812
6	5.71	821	37	0.152318	14	3.5448	4.7	3.5	5.71	825.8	13.9	0.143735
7	5.85	886	39.9	0.202783	4	0.629845	0.8	0.6	5.85	883.6	4.5	0.0797169
8	5.877	899	40.5	0.41429	12	8.67645	11.6	8.6	5.877	898.3	12.2	0.401174
9	5.991	953	42.9	0.235882	11	4.22168	5.6	4.2	5.991	953.3	11.4	0.209448
10	6.088	999	45	0.118647	9	1.9987	2.7	2	6.088	1000.6	9.2	0.122456
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	6.138	1023	46.1	0.0564265	5	0.436544	0.6	0.4	6.138	1024.3	4.8	0.0509823
12	6.447	1170	52.7	0.0933962	15	2.0999	2.8	2.1	6.447	1169.6	15.5	0.0765661
13	6.55	1219	54.9	0.129433	9	1.85675	2.5	1.8	6.55	1219.8	8.7	0.120073
14	6.652	1249	56.2	0.0893799	11	1.54301	2.1	1.5	6.652	1245.6	11.4	0.0764423
15	6.734	1273	57.3	0.319749	3	1.71144	2.3	1.7	6.734	1273.6	3.1	0.308433
16	6.795	1291	58.1	0.144953	6	1.52806	2	1.5	6.795	1290.3	5.7	0.150804
17	6.85	1307	58.8	0.295123	6	3.05009	4.1	3	6.85	1307	6.3	0.274099
18	7.017	1363	61.4	0.223895	7	2.87405	3.8	2.8	7.017	1361.5	7.3	0.220966
19	7.195	1423	64.1	0.088668	11	1.4404	1.9	1.4	7.195	1422.5	10.6	0.076904
20	7.35	1475	66.4	0.224282	9	3.41357	4.5	3.4	7.35	1474.7	8.8	0.219687
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
21	8.15	1759	79.2	0.0554305	15	1.48106	2	1.5	8.15	1760.2	15.2	0.0551541
22	8.45	1886	84.9	0.136746	13	3.14565	4.2	3.1	8.45	1885.5	13.3	0.133925
23	8.65	1955	88	0.114628	11	2.11378	2.8	2.1	8.65	1953.9	10.9	0.109005
24	9.3	2187	98.5	0.403843	11	7.2757	9.7	7.2	9.3	2183.4	11.1	0.371337

PeakCounter: 24

**DISPLAY GEL LANE LETTER:** Lane e
**DIGITAL REPORT GEL LANE:** 10
**LANE TYPE:** Specimen
**ACTUAL GEL LANE:** 12
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	3.55	79	3.6	0.0583498	7	0.671744	1.6	1.1	3.55	78.2	6.6	0.0574819
2	4.413	357	16.1	0.358113	7	4.50906	11	7.2	4.413	356.6	7.1	0.358808
3	5.591	767	34.5	0.0586597	6	0.555525	1.4	0.9	5.591	766.7	5.8	0.0538227
4	5.728	830	37.4	0.201815	11	3.37173	8.3	5.4	5.728	830.1	10.6	0.179421
5	5.881	901	40.6	0.496827	7	6.38888	15.6	10.2	5.881	900.8	7.2	0.497844
6	5.988	953	42.9	0.302071	6	3.2627	8	5.2	5.988	952.6	6.3	0.290643
7	6.079	997	44.9	0.185525	7	2.22011	5.4	3.6	6.079	995.6	7	0.178168
8	6.125	1019	45.9	0.0866461	7	0.960604	2.4	1.5	6.125	1020.6	7.3	0.0737632
9	6.418	1161	52.3	0.0717151	10	1.58884	3.9	2.5	6.418	1158.2	9.9	0.0905524
10	6.586	1235	55.6	0.131484	11	2.2184	5.4	3.5	6.586	1229.7	11.3	0.111166
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	6.664	1257	56.6	0.35615	3	1.94907	4.8	3.1	6.664	1256.6	3.2	0.338584
12	6.721	1273	57.3	0.182297	6	1.92026	4.7	3.1	6.721	1272.2	5.8	0.185519
13	6.786	1291	58.1	0.36608	5	3.36039	8.2	5.4	6.786	1291.3	5.4	0.35436
14	6.955	1345	60.6	0.273825	8	3.75031	9.2	6	6.955	1343.5	7.8	0.272888
15	7.136	1407	63.3	0.129651	11	2.21777	5.4	3.5	7.136	1406	11.3	0.110359
16	7.786	1635	73.6	0.0670613	16	1.92299	4.7	3.1	7.786	1631.1	16.4	0.066001

PeakCounter: 16

**DISPLAY GEL LANE LETTER:** Lane f
**DIGITAL REPORT GEL LANE:** 11
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 13
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	3.5	62	2.8	0.262624	18	8.40174	15.4	11.2	3.5	67.1	18.5	0.256774
2	4.55	403	18.2	0.234948	12	4.95086	9.1	6.6	4.55	403	12.2	0.229378
3	5.2	590	26.7	0.340275	9	5.62757	10.3	7.5	5.2	590	9.4	0.338612
4	5.85	886	40.1	0.413177	9	6.88591	12.6	9.2	5.85	886.2	9.2	0.421307
5	6.55	1231	55.7	0.296777	8	4.13448	7.6	5.5	6.55	1230.3	8.5	0.275555
6	6.85	1311	59.3	0.231271	8	3.20198	5.9	4.3	6.85	1310.2	8.1	0.221732
7	7.35	1486	67.3	0.267482	10	4.75841	8.7	6.3	7.35	1484.1	10.3	0.259858
8	8.15	1769	80.1	0.0858001	19	2.78026	5.1	3.7	8.15	1771.6	18.8	0.0835285
9	8.45	1888	85.5	0.162775	12	3.43576	6.3	4.6	8.45	1888.1	12.5	0.155482
10	8.65	1957	88.6	0.143641	11	2.69332	4.9	3.6	8.65	1956.9	10.7	0.141488
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	9.3	2189	99.1	0.412873	11	7.59405	13.9	10.1	9.3	2186.9	11.4	0.376172

PeakCounter: 11

Gel Banding Patterns Display in HTML using GIF stacking technique
(Uses Migration Position Normalized Data from above text peak reports)
Return to Lane Navigator Go to Gel Schematic Key

a b c d e f

MK SP MK CO SP MK

pI 3.50 label for gel schematic

pI 4.55 label for gel schematic

pI 5.20 label for gel schematic

pI 5.8 label for gel schematic

pI 6.55 label for gel schematic

pI 6.85 label for gel schematic

pI 7.35 label for gel schematic

pI 8.15 label for gel schematic

pI 8.45 label for gel schematic

pI 8.65 label for gel schematic

pI 9.30 label for gel schematic

pI 3.50 label for gel schematic

pI 4.55 label for gel schematic

pI 5.20 label for gel schematic

pI 5.8 label for gel schematic

pI 6.55 label for gel schematic

pI 6.85 label for gel schematic

pI 7.35 label for gel schematic

pI 8.15 label for gel schematic

pI 8.45 label for gel schematic

pI 8.65 label for gel schematic

pI 9.30 label for gel schematic

Key to Gel Schematic:

MK=pI Marker Lanes, pI bands are in red
SP=Specimen Lanes, Specimen bands are in blue
CO=Co-migrated Lanes of pI Markers and Specimen (pI bands are in red, Specimen bands are in blue)
Note: In co-migrated lanes, some pI Markers and Specimen bands may overlap in experiment. Red pI
band is reported when overlapped peak position is equal to nominal pI Marker position. Blue Specimen
band is reported when overlapped peak position deviates slightly from nominal pI Marker position.
Weak (<2% rel. intensity), Medium (2-6% rel. intensity), and Strong (>6% rel. intensity) band intensities indicated by gel schematic band thickness.
Return to Top of Gel Schematic

PeakCount: 90

HTML Forms TextArea Normalized Peak-Position Peak-Area for Copy-Paste events
3.500 14.900 4.550 7.200 5.200 9.600 5.850 10.300 6.550 7.300 6.850 6.700 7.350 9.500 8.150 5.900 8.450 7.600 8.650 5.200 9.300 15.800 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane a DIGITAL REPORT GEL LANE: 6 LANE TYPE: pI Markers ACTUAL GEL LANE: 8 PeakCount: 11 Return to Lane Navigator
3.541 1.700 4.449 10.400 5.615 1.300 5.736 6.800 5.884 16.500 5.991 9.900 6.080 3.800 6.125 4.300 6.424 3.100 6.449 2.000 6.607 3.800 6.686 5.200 6.743 4.500 6.800 8.400 6.977 8.800 7.137 4.600 7.772 4.800 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane b DIGITAL REPORT GEL LANE: 7 LANE TYPE: Specimen ACTUAL GEL LANE: 9 PeakCount: 17 Return to Lane Navigator
3.500 17.100 4.550 7.800 5.200 9.600 5.850 10.600 6.550 7.400 6.850 6.500 7.350 8.400 8.150 7.100 8.450 7.400 8.650 5.500 9.300 12.700 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane c DIGITAL REPORT GEL LANE: 8 LANE TYPE: pI Markers ACTUAL GEL LANE: 10 PeakCount: 11 Return to Lane Navigator
3.500 11.200 4.412 5.400 4.550 6.200 5.200 5.900 5.587 0.700 5.710 4.700 5.850 0.800 5.877 11.600 5.991 5.600 6.088 2.700 6.138 0.600 6.447 2.800 6.550 2.500 6.652 2.100 6.734 2.300 6.795 2.000 6.850 4.100 7.017 3.800 7.195 1.900 7.350 4.500 8.150 2.000 8.450 4.200 8.650 2.800 9.300 9.700 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane d DIGITAL REPORT GEL LANE: 9 LANE TYPE: Co-Migrated ACTUAL GEL LANE: 11 PeakCount: 24 Return to Lane Navigator
3.550 1.600 4.413 11.000 5.591 1.400 5.728 8.300 5.881 15.600 5.988 8.000 6.079 5.400 6.125 2.400 6.418 3.900 6.586 5.400 6.664 4.800 6.721 4.700 6.786 8.200 6.955 9.200 7.136 5.400 7.786 4.700 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane e DIGITAL REPORT GEL LANE: 10 LANE TYPE: Specimen ACTUAL GEL LANE: 12 PeakCount: 16 Return to Lane Navigator
3.500 15.400 4.550 9.100 5.200 10.300 5.850 12.600 6.550 7.600 6.850 5.900 7.350 8.700 8.150 5.100 8.450 6.300 8.650 4.900 9.300 13.900 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane f DIGITAL REPORT GEL LANE: 11 LANE TYPE: pI Markers ACTUAL GEL LANE: 13 PeakCount: 11 Return to Lane Navigator

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EXPERIMENTAL CONDITIONS: Gels were scanned at 600 d.p.i. in Adobe Photoshop 3.02 using a Sharp JX-325 Scanner with Film Scanner Attachment. Gels were analyzed for Densitometry, Intregration, and Migration Position Normalization in EXCEL 5.0 Format. *.xls files were imported into Paradox 7.0 to save as *.db files.

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Delphi 4.0 Generation of this Isoelectric Focusing (IEF) RFE PAGE 5 Tools in HTML
This HTML is automatically generated from a series of Paradox 7.0 database filesof IEF peak reports is controlled with a main RFEIEF Paradox 7.0 database withDelphi 3.0 front end (see Delphi 2.0 form) and back end for facile addition and modification of the Content of these RFE PAGE 5 HTML tools.
Contact Brad Tenge at btenge@ora.fda.gov for details.

RFE Contacts
A summary list of RFE contact personnel is available at http://www.cfsan.fda.gov/~frf/rfecon.html.

For isoelectric focusing laboratory experiments for fish species identification, you may contact

Brad Tenge at E-mail: btenge@ora.fda.gov
Ngoc-Lan Nguyen at E-mail: nnguyen@ora.fda.gov
Jim Barnett at E-mail: jbarnett@ora.fda.gov
Pat Rogers at E-mail: pxr@cfsan.fda.gov

For isoelectric focusing gel analysis and pattern recognition applications, you may contact

Brad Tenge at E-mail: btenge@ora.fda.gov
Ngoc-Lan Nguyen at E-mail: nnguyen@ora.fda.gov
Fred Fry at E-mail frf@vm.cfsan.fda.gov

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Fred Fry E-mail: frf@vm.cfsan.fda.gov

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Last updated on 2003-FEB-27 by bjt/frf/nln

Peak Report Data from Isoelectric Focusing for a specimen of: Oilfish(*) ---- Go to Display Gel Lane Navigator.

Peak Report Data from Isoelectric Focusing for a specimen of:
Oilfish(*) ---- Go to Display Gel Lane Navigator.