BLASTX+BEAUTY Search Results

WU-BLAST 2.0 search of the National Center for Biotechnology Information's NR Protein Database.

BEAUTY post-processing provided by the Human Genome Sequencing Center, Baylor College of Medicine.

BEAUTY Reference:
Worley KC, Culpepper P, Wiese BA, Smith RF. BEAUTY-X: enhanced BLAST searches for DNA queries. Bioinformatics 1998;14(10):890-1. Abstract

Worley KC, Wiese BA, Smith RF. BEAUTY: an enhanced BLAST-based search tool that integrates multiple biological information resources into sequence similarity search results. Genome Res 1995 Sep;5(2):173-84 Abstract



RepeatMasker repeats found in sequence:

No Repeats Found.

Reference:  Gish, Warren (1994-1997).  unpublished.
Gish, Warren and David J. States (1993).  Identification of protein coding
regions by database similarity search.  Nat. Genet. 3:266-72.

Notice: statistical significance is estimated under the assumption that the equivalent of one entire reading frame in the query sequence codes for protein and that significant alignments will involve only coding reading frames.

Query= 'E03F12_F12_12.ab1' (425 letters)

  Translating both strands of query sequence in all 6 reading frames

Database: nr 625,274 sequences; 197,782,623 total letters.



     Observed Numbers of Database Sequences Satisfying
    Various EXPECTation Thresholds (E parameter values)

        Histogram units:      = 3 Sequences     : less than 3 sequences

 EXPECTation Threshold
 (E parameter)
    |
    V   Observed Counts-->
  10000 646 151 |==================================================
   6310 495 138 |==============================================
   3980 357  57 |===================
   2510 300  73 |========================
   1580 227  68 |======================
   1000 159  57 |===================
    631 102  20 |======
    398  82  61 |====================
    251  21  12 |====
    158   9   3 |=
    100   6   0 |
   63.1   6   1 |:
   39.8   5   0 |
   25.1   5   1 |:
   15.8   4   2 |:
 >>>>>>>>>>>>>>>>>>>>>  Expect = 10.0, Observed = 2  <<<<<<<<<<<<<<<<<
   10.0   2   1 |:
   6.31   1   1 |:


                                                                     Smallest
                                                                       Sum
                                                     Reading  High  Probability
Sequences producing High-scoring Segment Pairs:        Frame Score  P(N)      N
gi|11528459|gb|AAG37286.1|(AF322247) E1 protein [Huma... -2    61  0.99      1
gi|11528445|gb|AAG37279.1|(AF322240) E1 protein [Huma... -2    59  0.9995    1

Use the and icons to retrieve links to Entrez:

E = Retrieve Entrez links (e.g., Medline abstracts, FASTA-formatted sequence reports).
R = Retrieve links to Related sequences (neighbors).
Use the icon (if present) to retrieve links to the Sequence Retrieval System (SRS).
Use the icon (if present) to retrieve links to the Ligand Enzyme and Chemical Compound Database .
Use the icon (if present) to retrieve links to the Protein Data Bank database.


to_Entrezto_Relatedto_Related >gi|11528459|gb|AAG37286.1|  (AF322247) E1 protein [Human papillomavirus]
            Length = 75

Frame -2 hits (HSPs):              _________________________________      
                        __________________________________________________
Database sequence:     |            |             |            |          | 75
                       0           20            40           60

  Minus Strand HSPs:

 Score = 61 (21.5 bits), Expect = 4.4, P = 0.99
 Identities = 13/49 (26%), Positives = 29/49 (59%), Frame = -2

Query:   268 GHV*IAMSCTH*CSVRIFFSPILIHNHL--HSNI---YIYAITTKYRYTN 134
             GH  +++ C H   ++I F P+L+ +++  HS I   Y+++    +++ N
Sbjct:    18 GHP-VSLDCKHKAPIQIKFPPLLVTSNIDIHSEIQYKYLHSRVQSFKFPN 66


to_Entrezto_Relatedto_Related >gi|11528445|gb|AAG37279.1|  (AF322240) E1 protein [Human papillomavirus]
            Length = 75

Frame -2 hits (HSPs):              _________________________________      
                        __________________________________________________
Database sequence:     |            |             |            |          | 75
                       0           20            40           60

  Minus Strand HSPs:

 Score = 59 (20.8 bits), Expect = 7.5, P = 1.0
 Identities = 12/49 (24%), Positives = 29/49 (59%), Frame = -2

Query:   268 GHV*IAMSCTH*CSVRIFFSPILIHNHL--HSNI---YIYAITTKYRYTN 134
             GH  +++ C H   +++ F P+LI +++  H  +   Y+++  T +++ N
Sbjct:    18 GHP-VSLDCKHKAPIQMKFPPLLITSNICIHEEVQYKYLHSRVTAFKFPN 66


Parameters:
  filter=none
  matrix=BLOSUM62
  V=50
  B=50
  E=10
  gi
  H=1
  sort_by_pvalue
  echofilter

  ctxfactor=5.96

  Query                        -----  As Used  -----    -----  Computed  ----
  Frame  MatID Matrix name     Lambda    K       H      Lambda    K       H
   Std.    0   BLOSUM62                                 0.318   0.135   0.401  
   +3      0   BLOSUM62        0.318   0.135   0.401    0.368   0.168   0.624  
               Q=9,R=2         0.244   0.0300  0.180     n/a     n/a     n/a
   +2      0   BLOSUM62        0.318   0.135   0.401    0.356   0.160   0.613  
               Q=9,R=2         0.244   0.0300  0.180     n/a     n/a     n/a
   +1      0   BLOSUM62        0.318   0.135   0.401    0.353   0.159   0.579  
               Q=9,R=2         0.244   0.0300  0.180     n/a     n/a     n/a
   -1      0   BLOSUM62        0.318   0.135   0.401    0.346   0.148   0.508  
               Q=9,R=2         0.244   0.0300  0.180     n/a     n/a     n/a
   -2      0   BLOSUM62        0.318   0.135   0.401    0.352   0.154   0.548  
               Q=9,R=2         0.244   0.0300  0.180     n/a     n/a     n/a
   -3      0   BLOSUM62        0.318   0.135   0.401    0.374   0.167   0.622  
               Q=9,R=2         0.244   0.0300  0.180     n/a     n/a     n/a

  Query
  Frame  MatID  Length  Eff.Length     E    S W   T  X   E2     S2
   +3      0      141       140       10.  73 3  12 22  0.12    33
                                                    30  0.11    36
   +2      0      141       140       10.  73 3  12 22  0.12    33
                                                    30  0.11    36
   +1      0      141       141       10.  73 3  12 22  0.12    33
                                                    30  0.11    36
   -1      0      141       140       10.  73 3  12 22  0.12    33
                                                    30  0.11    36
   -2      0      141       140       10.  73 3  12 22  0.12    33
                                                    30  0.11    36
   -3      0      141       140       10.  73 3  12 22  0.12    33
                                                    30  0.11    36


Statistics:

  Database:  /usr/local/dot5/sl_home/beauty/seqdb/blast/nr
    Title:  nr
    Release date:  unknown
    Posted date:  4:06 PM CST Feb 28, 2001
    Format:  BLAST
  # of letters in database:  197,782,623
  # of sequences in database:  625,274
  # of database sequences satisfying E:  2
  No. of states in DFA:  595 (59 KB)
  Total size of DFA:  182 KB (192 KB)
  Time to generate neighborhood:  0.02u 0.00s 0.02t  Elapsed: 00:00:00
  No. of threads or processors used:  6
  Search cpu time:  207.28u 0.90s 208.18t  Elapsed: 00:01:38
  Total cpu time:  207.32u 0.92s 208.24t  Elapsed: 00:01:38
  Start:  Wed Jan 16 21:31:26 2002   End:  Wed Jan 16 21:33:04 2002

Annotated Domains Database: March 14, 2000
Release Date: March 14, 2000