2007 Annual Report 1a.Objectives (from AD-416) Provide Natick soldier Center with data on the efficacy of repellent treatments for materials submitted to ARS. Funds received will offset costs incurred from the analysis of the efficiency of mosquito repellency in military uniforms of different materials and receiving different repellent treatments and binding agents. 1b.Approach (from AD-416) Develop novel assays and criteria for testing impregnated materials against sand flies, particularly Phlebotomine species involved in disease transmission in the Middle East. 3.Progress Report This report serves to document research conducted under a reimbursable agreement between ARS and Natick Soldier Center, Natick, MA to support research on the efficiency of mosquito repellency in military uniforms. Additional details of research can be found in the report for the parent project 6615-32000-043-00D “Control and Protection Tools for Integrated Pest Management of Mosquitoes and Filth Flies.” Research efforts by researchers at Natick Soldier Center and the Mosquito and Fly Research Unit (MFRU) were directed at the use of Individual Dynamic Absorption (IDA) kits for field permethrin treatment of U.S. Army combat uniforms (ACUs). Treated garments were laundered up to 50 wash cycles and these fabrics were analyzed for their ability to prevent mosquito bites. The results indicated that IDA kit treated ACUs provided greater than 90% bite protection against mosquito bites up to 50 wash cycles. Future work will consist of the examination of additional US Army fabrics used in uniforms. Progress is monitored and documented by reports issued to Natick at the completion of experiments, and meetings at the Armed Forces Pest Management Board.