RFE Fish Species Identification Isoelectric Focusing Tools

U.S. Food & Drug Administration
Seafood Products Research Center
Center for Food Safety & Applied Nutrition
Regulatory Fish Encyclopedia:
IEF Gel Lane Banding Pattern Peak Report Data

Peak Report Data from Isoelectric Focusing for a specimen of:
English Sole ---- Go to Display Gel Lane Navigator.

FDA Seafood List Scientific Name: Pleuronectes vetulus
FDA Seafood List Common Name: English Sole
FDA Seafood List Market Name: Sole
Unique IEF Gel Code: 19930817gel02
Unique Web Code: esg03Srr
Tissue Aliquot Code: pleuvetu 002 #1 Gel Analysis Conditions


Gel Image	Fish Image	Calibrated Gel	Comparison Gel

DISPLAY GEL LANE NAVIGATOR
RETURN TO TOP OF THIS DOCUMENT
Lane a pI Markers	Lane b Co-Migrated	Lane c Specimen	Lane d pI Markers	Lane e Co-Migrated	Lane f Specimen	Lane g pI Markers	Go to Gel Schematic

**DISPLAY GEL LANE LETTER:** Lane a
**DIGITAL REPORT GEL LANE:** 4
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 6
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	3.5	29	2.6	0.254403	5	2.17695	14.6	10.8	3.5	29.8	5.2	0.237714
2	4.55	174	15.6	0.156753	5	1.13047	7.6	5.6	4.55	174	4.5	0.140748
3	5.2	249	22.4	0.182228	4	1.34043	9	6.6	5.2	248.7	4.3	0.177008
4	5.85	383	34.4	0.323843	3	1.81851	12.2	9	5.85	382.8	3.2	0.32171
5	6.55	549	49.3	0.243868	4	1.49911	10.1	7.4	6.55	548.8	3.6	0.235304
6	6.85	584	52.4	0.042227	4	0.268481	1.8	1.3	6.85	583.6	3.9	0.0384492
7	7.35	679	61	0.110289	6	1.14472	7.7	5.7	7.35	678.6	6.2	0.104611
8	8.15	843	75.7	0.0613082	8	0.857803	5.8	4.3	8.15	844.3	8.5	0.0571872
9	8.45	913	82	0.0655909	6	0.679428	4.6	3.4	8.45	913.5	6.1	0.0627125
10	8.65	957	85.9	0.0588609	5	0.51632	3.5	2.6	8.65	957.2	5.2	0.0556891
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	9.3	1087	97.6	0.324095	7	3.42832	23.1	17	9.3	1085.8	6.6	0.294682

PeakCounter: 11

**DISPLAY GEL LANE LETTER:** Lane b
**DIGITAL REPORT GEL LANE:** 5
**LANE TYPE:** Co-Migrated
**ACTUAL GEL LANE:** 7
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	3.5	27	2.4	0.18035	11	4.06694	12	10.6	3.5	25.9	11.5	0.200034
2	4.076	106	9.5	0.277435	7	3.68761	10.9	9.6	4.076	104	6.8	0.305302
3	4.411	152	13.7	0.138592	5	1.14754	3.4	3	4.411	151.4	4.8	0.134187
4	4.55	171	15.4	0.108951	3	0.58281	1.7	1.5	4.55	170.4	3.4	0.0970702
5	4.62	179	16.1	0.0297046	7	0.408899	1.2	1.1	4.62	177.8	7.5	0.0308646
6	5.007	223	20	0.0684377	13	1.57563	4.6	4.1	5.007	224	13.3	0.0669705
7	5.2	245	22	0.113167	5	0.889052	2.6	2.3	5.2	245.7	4.7	0.106725
8	5.295	266	23.9	0.0448751	3	0.188509	0.6	0.5	5.295	266.1	2.6	0.0415139
9	5.368	282	25.3	0.0851356	9	1.44187	4.2	3.7	5.368	281.8	9.2	0.0880477
10	5.5	311	27.9	0.140111	3	0.835097	2.5	2.2	5.5	310.8	3.4	0.139319
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	5.595	332	29.8	0.437698	4	2.78818	8.2	7.2	5.595	332.3	3.6	0.435864
12	5.659	346	31.1	0.226255	5	1.88706	5.6	4.9	5.659	346	5	0.213407
13	5.741	364	32.7	0.0625171	9	1.02611	3	2.7	5.741	363.4	9.4	0.0619133
14	5.85	388	34.9	0.220743	3	0.933524	2.8	2.4	5.85	387.9	2.8	0.186183
15	5.901	400	35.9	0.113613	10	2.04779	6	5.3	5.901	399.6	10.5	0.110236
16	5.989	421	37.8	0.0524763	6	0.465345	1.4	1.2	5.989	422.7	5.5	0.0475475
17	6.318	499	44.8	0.0452913	11	0.734391	2.2	1.9	6.318	499.4	10.5	0.0393942
18	6.415	522	46.9	0.139709	3	0.654481	1.9	1.7	6.415	521.9	2.9	0.129409
19	6.55	554	49.8	0.126578	5	1.09752	3.2	2.9	6.55	552.8	5.2	0.118564
20	6.85	593	53.3	0.0542684	5	0.461724	1.4	1.2	6.85	594	5.5	0.0474307
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
21	7.011	623	56	0.00878911	5	0.101781	0.3	0.3	7.011	623	5.4	0.0105974
22	7.124	644	57.9	0.0259345	5	0.231738	0.7	0.6	7.124	643.5	5.1	0.025684
23	7.35	686	61.6	0.116599	5	0.97385	2.9	2.5	7.35	686	4.9	0.11242
24	8.15	849	76.3	0.0395131	17	1.29667	3.8	3.4	8.15	856	16.6	0.0440019
25	8.45	912	81.9	0.0413628	8	0.557974	1.6	1.4	8.45	911.8	7.9	0.0400724
26	8.65	956	85.9	0.0410121	6	0.452455	1.3	1.2	8.65	956.9	6.4	0.0398622
27	9.3	1085	97.5	0.301645	7	3.40113	10	8.8	9.3	1083.5	7	0.273966

PeakCounter: 27

**DISPLAY GEL LANE LETTER:** Lane c
**DIGITAL REPORT GEL LANE:** 6
**LANE TYPE:** Specimen
**ACTUAL GEL LANE:** 8
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	3.375	13	1.2	0.221762	5	1.97825	11.4	8	3.375	13.6	5.3	0.210516
2	4.388	151	13.6	0.152216	5	1.25232	7.2	5.1	4.388	151	4.8	0.148217
3	4.611	180	16.2	0.0262609	5	0.212966	1.2	0.9	4.611	179.8	4.9	0.0243208
4	4.943	218	19.6	0.0826281	11	1.45102	8.4	5.9	4.943	219.2	10.8	0.0756244
5	5.224	253	22.7	0.0257617	2	0.098906	0.6	0.4	5.224	252.4	2.1	0.0267396
6	5.269	263	23.6	0.0660145	2	0.247288	1.4	1	5.269	262.6	2.1	0.0672944
7	5.314	273	24.5	0.186801	4	1.13696	6.6	4.6	5.314	273.4	3.7	0.174058
8	5.474	309	27.8	0.172709	3	0.953928	5.5	3.9	5.474	308.4	3.2	0.166172
9	5.572	331	29.7	0.482003	3	2.6041	15.1	10.5	5.572	331.4	3.1	0.479584
10	5.63	344	30.9	0.237139	4	1.57965	9.1	6.4	5.63	344.2	4	0.222109
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	5.714	363	32.6	0.0634211	9	0.783556	4.5	3.2	5.714	361.2	8.6	0.0515542
12	5.865	397	35.7	0.1156	12	2.42433	14	9.8	5.865	396.2	11.7	0.116922
13	5.972	422	37.9	0.0795186	9	1.03359	6	4.2	5.972	424.2	9	0.0648655
14	6.39	520	46.7	0.131492	3	0.614899	3.6	2.5	6.39	520.4	2.8	0.125228
15	6.969	619	55.6	0.023365	10	0.426315	2.5	1.7	6.969	619.1	9.8	0.0244892
16	7.064	637	57.2	0.0558813	5	0.490588	2.8	2	7.064	636.1	5.4	0.0509381

PeakCounter: 16

**DISPLAY GEL LANE LETTER:** Lane d
**DIGITAL REPORT GEL LANE:** 7
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 9
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	3.5	36	3.2	0.115761	9	1.70951	11.6	8.4	3.5	37.5	9	0.107495
2	4.55	178	15.9	0.161309	5	1.19841	8.1	5.9	4.55	178.4	4.6	0.146016
3	5.2	253	22.7	0.17737	4	1.32823	9	6.6	5.2	253	4.3	0.173011
4	5.85	402	36	0.325913	3	1.99496	13.5	9.9	5.85	402	3.4	0.326855
5	6.55	564	50.5	0.227444	4	1.51057	10.2	7.5	6.55	563.9	4	0.212588
6	6.85	603	54	0.0780905	6	0.720196	4.9	3.6	6.85	603	6.1	0.0668717
7	7.35	699	62.6	0.110229	6	1.13976	7.7	5.6	7.35	697.8	6.4	0.10005
8	8.15	862	77.2	0.0392525	9	0.638642	4.3	3.2	8.15	863.8	9.3	0.0387502
9	8.45	925	82.9	0.064672	7	0.763697	5.2	3.8	8.45	925.2	6.8	0.0637293
10	8.65	967	86.6	0.0486016	6	0.487091	3.3	2.4	8.65	967.6	6.3	0.0436678
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	9.3	1091	97.8	0.28999	7	3.29792	22.3	16.3	9.3	1088.8	7.1	0.260531

PeakCounter: 11

**DISPLAY GEL LANE LETTER:** Lane e
**DIGITAL REPORT GEL LANE:** 8
**LANE TYPE:** Co-Migrated
**ACTUAL GEL LANE:** 10
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	3.5	21	1.9	0.176185	9	2.40249	7.3	6.2	3.5	22.2	8.6	0.157528
2	4.084	110	9.9	0.327061	7	4.40337	13.4	11.4	4.084	112.5	7	0.354445
3	4.425	162	14.6	0.189674	5	1.64088	5	4.2	4.425	161.5	5.1	0.183081
4	4.55	181	16.3	0.116672	6	1.03734	3.2	2.7	4.55	181.8	6	0.0972013
5	5.012	235	21.1	0.0671251	12	1.40158	4.3	3.6	5.012	236.8	12.5	0.0633779
6	5.2	257	23.1	0.107169	4	0.695497	2.1	1.8	5.2	257.1	3.8	0.10299
7	5.253	269	24.2	0.0210226	2	0.0766035	0.2	0.2	5.253	268.3	2	0.0216433
8	5.292	278	25	0.0736509	2	0.263211	0.8	0.7	5.292	278.1	2	0.0747024
9	5.341	289	26	0.159835	5	1.38214	4.2	3.6	5.341	290	5.2	0.150157
10	5.49	323	29	0.147571	4	1.03848	3.2	2.7	5.49	322	4	0.144719
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	5.586	345	31	0.481123	3	2.90858	8.9	7.5	5.586	344.7	3.4	0.486197
12	5.639	357	32.1	0.245649	4	1.4967	4.6	3.9	5.639	357	3.7	0.227949
13	5.701	371	33.4	0.0707839	11	1.34215	4.1	3.5	5.701	371.6	11	0.0689268
14	5.85	405	36.4	0.211697	4	1.59324	4.9	4.1	5.85	405	4.4	0.202257
15	5.89	414	37.2	0.108379	5	0.939403	2.9	2.4	5.89	413.4	5.1	0.104727
16	5.967	431	38.8	0.0671939	11	1.14952	3.5	3	5.967	430.1	11.1	0.0583045
17	6.308	507	45.6	0.0281672	2	0.0216128	0.1	0.1	6.308	507.3	1.6	0.00776788
18	6.339	514	46.2	0.0260525	18	0.679281	2.1	1.8	6.339	513.4	18	0.0212917
19	6.424	533	47.9	0.154434	2	0.479816	1.5	1.2	6.424	533	1.9	0.143945
20	6.55	561	50.4	0.0756389	7	0.926857	2.8	2.4	6.55	559.1	7.2	0.0723482
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
21	6.85	602	54.1	0.0539714	5	0.437379	1.3	1.1	6.85	601.9	5.1	0.048363
22	7.087	647	58.2	0.0117849	5	0.114122	0.3	0.3	7.087	647.7	4.8	0.0134345
23	7.35	697	62.7	0.108688	5	0.949177	2.9	2.5	7.35	696.4	4.9	0.108289
24	8.15	863	77.6	0.0871507	18	1.13474	3.5	2.9	8.15	863.4	18	0.0355678
25	8.45	921	82.8	0.0689596	8	0.923325	2.8	2.4	8.45	921.6	7.9	0.0663574
26	8.65	965	86.8	0.0321858	6	0.380676	1.2	1	8.65	963.2	5.9	0.0366232
27	9.3	1086	97.7	0.261953	7	3.02953	9.2	7.8	9.3	1083.8	7.2	0.238713

PeakCounter: 27

**DISPLAY GEL LANE LETTER:** Lane f
**DIGITAL REPORT GEL LANE:** 9
**LANE TYPE:** Specimen
**ACTUAL GEL LANE:** 11
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	3.425	17	1.5	0.214309	7	2.71933	10.3	9.2	3.425	16.6	6.9	0.221579
2	4.098	116	10.4	0.327418	7	4.64901	17.6	15.7	4.098	118.8	7.5	0.349962
3	4.472	171	15.3	0.207364	5	1.82109	6.9	6.1	4.472	170.5	5.1	0.200126
4	4.702	200	17.9	0.0259792	7	0.413315	1.6	1.4	4.702	202.7	6.9	0.0339485
5	5.135	250	22.4	0.0688609	17	2.07822	7.9	7	5.135	254.7	17.1	0.0685569
6	5.301	280	25.1	0.0359227	2	0.0907864	0.3	0.3	5.301	279.6	2.2	0.0232075
7	5.341	289	25.9	0.0873584	2	0.33078	1.3	1.1	5.341	289.7	2.3	0.0812568
8	5.372	296	26.5	0.0353836	2	0.146888	0.6	0.5	5.372	296.8	2.4	0.0344387
9	5.43	309	27.7	0.153026	5	1.39048	5.3	4.7	5.43	308.9	5.2	0.151902
10	5.542	334	30	0.217028	3	1.10111	4.2	3.7	5.542	333.5	2.8	0.221116
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	5.631	354	31.7	0.486821	3	2.53851	9.6	8.6	5.631	354.2	3	0.480314
12	5.694	368	33	0.283414	4	1.90453	7.2	6.4	5.694	367.7	4.1	0.265182
13	5.774	386	34.6	0.0809795	7	0.950466	3.6	3.2	5.774	385.8	6.9	0.0776827
14	5.867	407	36.5	0.109882	3	0.278367	1.1	0.9	5.867	406.6	2.5	0.0626782
15	5.911	417	37.4	0.126823	10	2.11794	8	7.2	5.911	416.6	9.9	0.121309
16	6.015	441	39.6	0.0523206	5	0.414546	1.6	1.4	6.015	439.9	4.8	0.0484089
17	6.058	451	40.4	0.0406245	5	0.233753	0.9	0.8	6.058	451.5	4.5	0.0291312
18	6.314	510	45.7	0.0514674	17	1.35361	5.1	4.6	6.314	513	17.1	0.0447783
19	6.409	532	47.7	0.181707	2	0.657262	2.5	2.2	6.409	532	2.2	0.166674
20	6.972	627	56.2	0.0312627	11	0.63385	2.4	2.1	6.972	627.5	11.4	0.0312562
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
21	7.072	646	57.9	0.0666321	6	0.596059	2.3	2	7.072	645.5	5.5	0.0610682

PeakCounter: 21

**DISPLAY GEL LANE LETTER:** Lane g
**DIGITAL REPORT GEL LANE:** 10
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 12
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	3.5	33	3	0.145434	10	2.33062	14.7	10.1	3.5	33.7	9.6	0.13642
2	4.55	182	16.3	0.160073	5	1.27596	8.1	5.5	4.55	181.7	5	0.143842
3	5.2	256	22.9	0.174495	5	1.37986	8.7	6	5.2	256.1	4.5	0.171342
4	5.85	399	35.8	0.337901	3	1.80708	11.4	7.8	5.85	399.3	3.1	0.333
5	6.55	566	50.7	0.237032	4	1.57993	10	6.8	6.55	565.7	4	0.221806
6	6.85	604	54.1	0.0620762	6	0.601542	3.8	2.6	6.85	604.3	6.3	0.0535952
7	7.35	698	62.5	0.106798	6	1.10489	7	4.8	7.35	697.2	6.3	0.0986589
8	8.15	865	77.5	0.0509047	9	0.806466	5.1	3.5	8.15	863.8	9.2	0.0492815
9	8.45	926	83	0.0767911	7	0.904024	5.7	3.9	8.45	926.6	6.8	0.074945
10	8.65	969	86.8	0.0475814	6	0.521144	3.3	2.3	8.65	968.3	6.4	0.0458815
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	9.3	1092	97.8	0.281489	8	3.51969	22.2	15.3	9.3	1089.8	7.8	0.255247

PeakCounter: 11

Gel Banding Patterns Display in HTML using GIF stacking technique
(Uses Migration Position Normalized Data from above text peak reports)
Return to Lane Navigator Go to Gel Schematic Key

a b c d e f g

MK CO SP MK CO SP MK

pI 3.50 label for gel schematic

pI 4.55 label for gel schematic

pI 5.20 label for gel schematic

pI 5.8 label for gel schematic

pI 6.55 label for gel schematic

pI 6.85 label for gel schematic

pI 7.35 label for gel schematic

pI 8.15 label for gel schematic

pI 8.45 label for gel schematic

pI 8.65 label for gel schematic

pI 9.30 label for gel schematic

pI 3.50 label for gel schematic

pI 4.55 label for gel schematic

pI 5.20 label for gel schematic

pI 5.8 label for gel schematic

pI 6.55 label for gel schematic

pI 6.85 label for gel schematic

pI 7.35 label for gel schematic

pI 8.15 label for gel schematic

pI 8.45 label for gel schematic

pI 8.65 label for gel schematic

pI 9.30 label for gel schematic

Key to Gel Schematic:

MK=pI Marker Lanes, pI bands are in red
SP=Specimen Lanes, Specimen bands are in blue
CO=Co-migrated Lanes of pI Markers and Specimen (pI bands are in red, Specimen bands are in blue)
Note: In co-migrated lanes, some pI Markers and Specimen bands may overlap in experiment. Red pI
band is reported when overlapped peak position is equal to nominal pI Marker position. Blue Specimen
band is reported when overlapped peak position deviates slightly from nominal pI Marker position.
Weak (<2% rel. intensity), Medium (2-6% rel. intensity), and Strong (>6% rel. intensity) band intensities indicated by gel schematic band thickness.
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PeakCount: 124

HTML Forms TextArea Normalized Peak-Position Peak-Area for Copy-Paste events
3.500 14.600 4.550 7.600 5.200 9.000 5.850 12.200 6.550 10.100 6.850 1.800 7.350 7.700 8.150 5.800 8.450 4.600 8.650 3.500 9.300 23.100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane a DIGITAL REPORT GEL LANE: 4 LANE TYPE: pI Markers ACTUAL GEL LANE: 6 PeakCount: 11 Return to Lane Navigator
3.500 12.000 4.076 10.900 4.411 3.400 4.550 1.700 4.620 1.200 5.007 4.600 5.200 2.600 5.295 0.600 5.368 4.200 5.500 2.500 5.595 8.200 5.659 5.600 5.741 3.000 5.850 2.800 5.901 6.000 5.989 1.400 6.318 2.200 6.415 1.900 6.550 3.200 6.850 1.400 7.011 0.300 7.124 0.700 7.350 2.900 8.150 3.800 8.450 1.600 8.650 1.300 9.300 10.000 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane b DIGITAL REPORT GEL LANE: 5 LANE TYPE: Co-Migrated ACTUAL GEL LANE: 7 PeakCount: 27 Return to Lane Navigator
3.375 11.400 4.388 7.200 4.611 1.200 4.943 8.400 5.224 0.600 5.269 1.400 5.314 6.600 5.474 5.500 5.572 15.100 5.630 9.100 5.714 4.500 5.865 14.000 5.972 6.000 6.390 3.600 6.969 2.500 7.064 2.800 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane c DIGITAL REPORT GEL LANE: 6 LANE TYPE: Specimen ACTUAL GEL LANE: 8 PeakCount: 16 Return to Lane Navigator
3.500 11.600 4.550 8.100 5.200 9.000 5.850 13.500 6.550 10.200 6.850 4.900 7.350 7.700 8.150 4.300 8.450 5.200 8.650 3.300 9.300 22.300 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane d DIGITAL REPORT GEL LANE: 7 LANE TYPE: pI Markers ACTUAL GEL LANE: 9 PeakCount: 11 Return to Lane Navigator
3.500 7.300 4.084 13.400 4.425 5.000 4.550 3.200 5.012 4.300 5.200 2.100 5.253 0.200 5.292 0.800 5.341 4.200 5.490 3.200 5.586 8.900 5.639 4.600 5.701 4.100 5.850 4.900 5.890 2.900 5.967 3.500 6.308 0.100 6.339 2.100 6.424 1.500 6.550 2.800 6.850 1.300 7.087 0.300 7.350 2.900 8.150 3.500 8.450 2.800 8.650 1.200 9.300 9.200 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane e DIGITAL REPORT GEL LANE: 8 LANE TYPE: Co-Migrated ACTUAL GEL LANE: 10 PeakCount: 27 Return to Lane Navigator
3.425 10.300 4.098 17.600 4.472 6.900 4.702 1.600 5.135 7.900 5.301 0.300 5.341 1.300 5.372 0.600 5.430 5.300 5.542 4.200 5.631 9.600 5.694 7.200 5.774 3.600 5.867 1.100 5.911 8.000 6.015 1.600 6.058 0.900 6.314 5.100 6.409 2.500 6.972 2.400 7.072 2.300 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane f DIGITAL REPORT GEL LANE: 9 LANE TYPE: Specimen ACTUAL GEL LANE: 11 PeakCount: 21 Return to Lane Navigator
3.500 14.700 4.550 8.100 5.200 8.700 5.850 11.400 6.550 10.000 6.850 3.800 7.350 7.000 8.150 5.100 8.450 5.700 8.650 3.300 9.300 22.200 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane g DIGITAL REPORT GEL LANE: 10 LANE TYPE: pI Markers ACTUAL GEL LANE: 12 PeakCount: 11 Return to Lane Navigator

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EXPERIMENTAL CONDITIONS: Gels were scanned at 600 d.p.i. in Adobe Photoshop 3.02 using a Sharp JX-325 Scanner with Film Scanner Attachment. Gels were analyzed for Densitometry, Intregration, and Migration Position Normalization in EXCEL 5.0 Format. *.xls files were imported into Paradox 7.0 to save as *.db files.

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Delphi 4.0 Generation of this Isoelectric Focusing (IEF) RFE PAGE 5 Tools in HTML
This HTML is automatically generated from a series of Paradox 7.0 database filesof IEF peak reports is controlled with a main RFEIEF Paradox 7.0 database withDelphi 3.0 front end (see Delphi 2.0 form) and back end for facile addition and modification of the Content of these RFE PAGE 5 HTML tools.
Contact Brad Tenge at btenge@ora.fda.gov for details.

RFE Contacts
A summary list of RFE contact personnel is available at http://www.cfsan.fda.gov/~frf/rfecon.html.

For isoelectric focusing laboratory experiments for fish species identification, you may contact

Brad Tenge at E-mail: btenge@ora.fda.gov
Ngoc-Lan Nguyen at E-mail: nnguyen@ora.fda.gov
Jim Barnett at E-mail: jbarnett@ora.fda.gov
Pat Rogers at E-mail: pxr@cfsan.fda.gov

For isoelectric focusing gel analysis and pattern recognition applications, you may contact

Brad Tenge at E-mail: btenge@ora.fda.gov
Ngoc-Lan Nguyen at E-mail: nnguyen@ora.fda.gov
Fred Fry at E-mail frf@vm.cfsan.fda.gov

For CFSAN web consultation and posting, you may contact

Fred Fry E-mail: frf@vm.cfsan.fda.gov

Thank you for using the Regulatory Fish Encyclopedia (RFE)!

RFE Species Browse | RFE Tutorial | Seafoods | CFSAN

Last updated on 2003-FEB-27 by bjt/frf/nln

Peak Report Data from Isoelectric Focusing for a specimen of: English Sole ---- Go to Display Gel Lane Navigator.

Peak Report Data from Isoelectric Focusing for a specimen of:
English Sole ---- Go to Display Gel Lane Navigator.