http://www.pgec.usda.gov/Hake/inflorescence.html
|
Maize flowers are organized in spikelets as are all members of the grass family. Two florets are found in the spikelet. This drawing, courtesy of Bruce Veit, is of a spikelet in the tassel. | Normal tassels have long branches at their base and spikelets at the top. | Scanning electron microscopy shows the developing spikelet meristems on the tassel. (photo courtesy of George Chuck) | ||
A thick tassel dwarf1 mutant tassel is shown on the left with a normal tassel on the right. Notice the increased spikelet density in the middle of the tassel rachis. |
|
Uncontrolled proliferation of the ear inflorescence leads to fasciation and abnormal kernel rowing. Top, td1 mutant has some reverse germ orientation and a fasciated ear tip. (Ears were open-pollinated and do not reflect a difference in fertility.) |
||
ramosa2 mutants have spikelet pair meristems with increased indeterminacy. As a result, ears are often branched at the base and the rows are disorganized |
An SEM of a ra2 ear showing branches at the base |
| ||
branched silkless 1 (bd1) mutants (left) show inflorescence branch proliferation.
A wild type ear is on the right |
SEM of bd1 mutant ear showing that the spikelet meristem becomes branch-like | Longitudinal (left) and whole mount (right) sections showing in situ hibridization of bd1.
Transcript expression is seen at the base of the spikelet meristem. |
Bortiri, E., Chuck, G., Vollbrecht, E., Rocheford, T., Martienssen, T., and Hake, S. (2006). ramosa2 encodes a LATERAL ORGAN BOUNDARY domain protein that determines the fate of stem cells in branch meristems of maize. The Plant Cell. Online [Abstract][PDF]
Kozaki, A., Hake, S., and Colasanti, J. (2004). The maize ID1 flowering
time regulator is a zinc finger protein with novel DNA binding
properties. Nucleic Acids Research 32:1710-1720. Hake, S. and Rocheford, T. (2004) Exploiting quantitative trait loci in
gene discovery. Genes and Development 18:597-601. Magnani E., Sjolander K., Hake S. (2004) From endonucleases to
transcription factors: evolution of the AP2 DNA binding domain in
plants. Plant Cell 16:2265-77. Laudencia-Chingcuanco, D. and Hake, S. (2002) The indeterminate floral apex1 gene regulates meristem determinacy and identity in the maize inflorescence. Development. 129:2629-2638. Hubbard, L. McSteen, P. Doebley, J. and Hake, S. (2002) Expression patterns and mutant phenotypes of teosinte brached1 correlate with growth suppression in maize and teosinte Genetics 162:1927-1935 Chuck G, Muszynski M, Kellogg E, Hake S, Schmidt RJ. (2002) The control of spikelet meristem identity by the branched silkless1 gene in maize. Science. 298:1238-41. Taguchi Shiobara, F., Yuan, Z. Hake, S. and Jackson, D. (2001) The fasciated ear2 gene encodes a leucine rich repeat receptor like protein that regulates shoot meristem proliferation in maize. Genes and Development 15:2755-2766. McSteen, P. and Hake, S. (2001) barren inflorescence2 regulates axillary meristem development in the maize inflorescence. Development 128:2881-2891. Veit, B., Briggs, S., Schmidt, R. J., Yanofsky, M. F., and Hake, S. (1998) Regulation of leaf initiation by the terminal ear1 gene of maize. Nature 393: 166-168. Chuck, G. Meeley, R. B. and Hake, S. (1998) The control of maize spikelet meristem fate by the APETELA2-like gene indeterminate spikelet1. Genes and Development 12:1145-1154. McSteen, P. and Hake, S. (1998) Genetic control of plant development. Current Opinion in Biotechnology 9:189-195. Veit, B. Schmidt, R.J., Hake, S. & Yanofsky, M.F. 1993. Maize floral development - new genes and old mutants. Plant Cell 5: 1205-1215. Schmidt, R.J., Veit, B., Mandel, M.A., Mena, M., Hake, S. & Yanofsky, Y. 1993. Identification and molecular characterization of ZAG1, the maize homolog of the Arabidopsis floral homeotic gene AGAMOUS. 1993. Plant Cell 5:729-737. X
The Hake Lab
knox genes
Inflorescence development
Lab Members
Links