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Preface Chapter 1 Nomenclature Reaction Components Kinetic Mechanism Kinetic Constants Microscopic Rate Constants Macroscopic Rate Constants Chapter 2 Introduction to Kinetics Chemical Kinetics First-Order Kinetics Second-Order Kinetics Pseudo-First-Order Kinetics Saturation Kinetics Temperature Dependence of Kinetic Parameters Steady-State Enzyme Kinetics Initial Rate Steady State The Michaelis-Menten/Briggs-Haldane Equation Overall Rate Equation Presentation of Initial Rate Data Chapter 3 Enzyme Assays Assays with Reduced Pyridine Nucleotides Fixed Time Assays Coupled Assays Analysis of Time Courses Chapter 4 Derivation of Initial Velocity Rate Equations and Data Processing Derivation of Rate Equations Algebraic Solution King-Altman Method Alternative Reaction Pathways Conversion of the Initial Velocity Rate Equation to Kinetic Constants Net Rate Constant Method Rapid Equilibrium Approximation Derivation of Equations for Isotope Exchange Derivation of Equations for Isotope Effects Shorthand Notation for Rate Constants Data Processing Chapter 5 Initial Velocity Studies in the Absence of Added Inhibitors Uni Bi Enzyme Reactions Bireactant Enzyme Reactions Ordered Sequential Mechanisms Equilibrium Ordered Mechanism Steady-State Ordered Mechanism Theorell-Chance Mechanism Random Sequential Reactions Dependence of Km on Reactant Concentration Ping Pong Reactions Determination of Vmax Can Sequential and Ping Pong Mechanisms Resemble One Another? Crossover Point Analysis Terreactant Enzyme Mechanisms No Constant Term-Ping Pong Mechanisms Constant Term Present-Sequential Mechanisms Methods for Telling Which Denominator Terms Are Missing Haldane Relationship Alternate Substrate Studies Kinetics of Metal Ions Cooperativity and Allosterism Positive Cooperativity Negative Cooperativity The Hill Plot Allosterism Kinetic Mechanism of Regulation Transmission of Allosteric Effects-Coupling of Active and Allosteric Sites Practical Considerations From Data to Interpretation Chapter 6 Initial Velocity Studies: Presence of Added Inhibitors Irreversible Step Types of Inhibition Competitive Inhibition Noncompetitive Inhibition Uncompetitive Inhibition Product Inhibition Uni Bi Reaction Mechanisms Uni Bi Steady-State Ordered Uni Bi Rapid Equilibrium Random Rules for Predicting Product Inhibition Patterns Bireactant Enzyme Mechanisms Ordered Kinetic Mechanisms Steady-State Ordered Theorell-Chance Equilibrium Ordered Random Kinetic Mechanisms Rapid Equilibrium Random with EBQ Dead-End Complex Steady-State Random Ping Pong Mechanisms Classical (One-Site) Ping Pong Mechanism Nonclassical (Two-Site) Ping Pong Mechanism Alternate Product Inhibition Dead-End Inhibition Rules for Predicting Dead-End Inhibition Patterns Combination of Inhibitor with More Than a Single Enzyme Form Mixed Product and Dead-End Inhibition Multiple Combinations of a Dead-End Inhibitor Substrate Inhibition Complete Substrate Inhibition Partial Substrate Inhibition Induced Substrate Inhibition Alternate Substrate Inhibition Ping Pong Mechanism Sequential Mechanisms Double Inhibition Slow Binding and Tight Binding Inhibition Slow Binding Inhibition Effect of a Reversible Inhibitor on a Slow Binding Inhibitor Tight Binding Inhibition Slow Tight Binding Inhibition Some Practical Considerations Chapter 7 Pre-Steady-State and Relaxation Kinetics Reactant Concentration in Excess of Enzyme Concentration Irreversible First-Order Reactions Reversible First-Order Reactions Consecutive First-Order Reactions Parallel First-Order Reactions Burst in a Time Course Reactant Concentration Comparable to Enzyme Concentration Temperature Jump Methods of Pre-Steady-State Analysis Rapid Spectral Acquisition Single Wavelength Methods Stopped Flow Rapid Quench Relaxation Methods Chapter 8 Isotopic Probes of Kinetic Mechanism Isotopic Exchange Isotopic Exchange at Equilibrium Ping Pong Exchange Patterns Exchange at Equilibrium in Sequential Mechanisms Isotope Exchange Not at Equilibrium Oversaturation and Iso Mechanisms Countertransport of Label Positional Isotopic Exchange (PIX) Determination of Stickiness Isotope Partitioning Experimental Data Analysis Theory Theoretical Limits Variation of V/K with Viscosity Chapter 9 Isotope Effects as a Probe of Mechanism Types of Isotope Effects Nomenclature Measurement of Kinetic Isotope Effects Direct Comparison of Initial Rates Equilibrium Perturbation Internal Competition Remote Label Method Types of Isotope Effects Equilibrium Isotope Effects Equations for Isotope Effects Calculation of Dissociation Constant Isotope Effects on More Than One Step Determination of Intrinsic Isotope Effects Northrop's Method Multiple Isotope Effect Method Multiple Isotope Effects in Stepwise Mechanisms Intermediate Partitioning Reactant Dependence of Isotope Effects Substrate Dependence of Isotope Effects in Bireactant Sequential Mechanisms Ordered Mechanisms Random Mechanisms Ping Pong Mechanisms Substrate Dependence of Isotope Effects in Terreactant and Higher Order Mechanisms Product Dependence of Isotope Effects Ordered Kinetic Mechanisms Random Kinetic Mechanisms Ping Pong Kinetic Mechanisms Isotope Effects as a Probe of Regulatory Mechanism Isotope Effects as a Probe of Chemical Mechanism Isotope Effects as a Probe of Transition-State Structure Formate Dehydrogenase Alcohol Dehydrogenase Glutamate Mutase Acyl and Phosphoryl Transfers Phosphoryl Transfer Glycosyl Transferases Binding Isotope Effects Transient-State Kinetic Isotope Effects Chapter 10 pH Dependence of Kinetic Parameters and Isotope Effects pH-Rate Studies Single Buffers with Overlap Mixed Buffer Systems pH Dependence of Equilibrium Dissociation Constant pH Dependence of V/K Activity Lost at Low pH Activity Lost at Low pH and pK1 above pH 7.3 Activity Lost at High pH Activity Lost at High pH and pK1 Less Than 7.3 V/K Profile Decreases at both Low and High pH V/K Profiles That Show 2 pKs at Low or High pH Identifying the Groups Seen in V/K Profiles pH Dependence of Vmax V Profile with a Sticky Substrate and Proton pH Dependence of Vmax in a Ping Pong Mechanism Metal Ion Binding pKi Profiles for Metal Ions pH Dependence of Isotope Effects pH-Dependent Step is Sensitive to Isotopic Substitution Random Addition of Proton and Substrate to Enzyme Dead-End Protonation of Enzyme Dead-End Protonation of Enzyme and Enzyme-Reactant Complex Dead-End Formation of Protonated Enzyme-Reactant Complex DV in a Ping Pong Mechanism pH- and Isotope-Sensitive Steps Differ Appendices A1. King and Altman Patterns and Distribution Equations 367 A2. Rate Equations, Definitions of Kinetic and Inhibition Constants, Haldanes, Distribution Equations, and Rate Constant Calculations for a Number of Multireactant Mechanisms
Library of Congress Subject Headings for this publication:
Enzyme kinetics.
Enzymes -- metabolism.
Kinetics.