EC-NUMBER
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1.1.1.274
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ENZYME-NAME
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2,5-didehydrogluconate reductase
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ENZYME-SYSNAME
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This is a key enzyme in the microbial production of ascorbate. Can also reduce ethyl 2-methylacetoacetate stereoselectively to ethyl (2R)-methyl-(3S)-hydroxybutanoate and can also accept some other beta-keto esters. The identification of a bacterial beta-keto ester reductase as a 2,5-didehydrogluconate reductase links two previously separate areas of biocatalysis, i.e., asymmetric carbonyl reduction and microbial vitamin C production.
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ENZYME-CLASS
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Oxidoreductases Acting on the CH-OH group of donors With NAD+ or NADP+ as acceptor
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SYNONYMS
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2,5-didehydrogluconate reductase;;
2,5-diketo-D-gluconate reductase;;
YqhE reductase;
>>more synonyms
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REACTION
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2-dehydro-D-gluconate + NADP+ = 2,5-didehydro-D-gluconate + NADPH + H+ [RN:R05823]
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PATHWAY
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EMP-PATHWAYS:
NO EMP PATHWAY FOUND
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KEGG-PATHWAYS:
NO KEGG PATHWAY FOUND
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GO_TERM
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GO:0050580:2,5-didehydrogluconate reductase activity
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ENZYME-COMMENT
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2-dehydro-D-gluconate:NADP+ 2-oxidoreductase
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