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Title Regulation of biosynthesis of secondary metabolites. XV. Isolation and characterization of auxotrophic mutants in Streptomyces aureofaciens
Creator/Author Blumauerova, M. ; Ismail, A.A. ; Hostalek, Z. ; Callieri, D.A.
Publication Date1973 Jan 01
OSTI IdentifierOSTI ID: 4357922
Other Number(s)CODEN: FOMIA
Resource TypeJournal Article
Resource RelationFolia Microbiol. (Prague), v. 18, no. 6, pp. 474-491
Research OrgInst. of Microbiology, Prague
SubjectN48310 --Life Sciences--Radiation Effects on Microorganisms--Basic Studies; MUTATIONS-- RADIOINDUCTION;STREPTOMYCES-- MUTATIONS;ARGININE;BIOLOGICAL RADIATION EFFECTS;BIOSYNTHESIS;CARBONIC ACID DERIVATIVES;METHIONINE;NITRO COMPOUNDS;NITROSO COMPOUNDS;ORGANIC NITROGEN COMPOUNDS;PHOTON BEAMS;PURINES;RADIATION DOSES;SERINE;ULTRAVIOLET RADIATION
Description/Abstract Auxotrophic mutants of Streptomyces aureofaciens, necessary for a further genetic analysis, were obtained by treating standard strains and their mutants (differing by their biosynthetic activities) with uv-radiation and N-methyl-N'-nitro-N-nitrosoguanidine (MNG).Mean frequencies of induced mutations varied within 0.001 to 0.18% depending on the strain and experimental conditions.Doses of uv radiation and MNG yielding 0.1 to 0.9% and 0.001 to 0.0009% survival, respectively, were found to be most effective.The technique of subsequent enrichment was found to be more useful for the detection of mutants than the technique of total isolation.Most isolated auxotrophs required arginine.Among other growth factors, methionine, purine bases and serine (or glycine) were frequently required.A new modification of the enrichment technique utilizing a synthetic arginine-free medium was worked out for the exclusion of the arg mutants.The nutritional deficiency was in most auxotrophs associated with a decrease or a complete loss of the ability to produce secondary metabolites.Difficulties caused by unequal mutation patterns and high unstability of mutants as well as presumable loci of certain genetic blocks leading to auxotrophy are discussed.(auth)
LanguageEnglish
System Entry Date2001 Jun 03

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