Taruscio D, Manuelidis L; International Conference on AIDS.
Int Conf AIDS. 1991 Jun 16-21; 7: 132 (abstract no. M.A.1163).
Yale Medical School, New Haven, CT, USA
We analyzed the chromosomal integration sites of a human endogenous retrovirus (HERV). HERV is present in 50-100 copies in the genome and is related to MLV. Using high resolution in-situ hybridization, HERV integration sites were localized with respect to chromosomal bands containing high concentrations of Alu repeats. Alu PCR products from unfractionated human DNA labeled with digoxigenin, and biotinylated HERV sequences were used as probes, and simultaneously detected with two different fluorochromes. HERV sequences were confined to 29 chromosomal sites and approximately 90% of these were unambiguously located in Giemsa-light and Alu rich (presumably early replicating) chromosomal domains. Moreover, approximately 70% of the resolved HERV integration sites corresponded to known breakpoint and/or recombination hotspots. The quantitative data suggested that only 13 or fewer sites could potentially contain full length copies of 8.8kb. Remarkably, chromosome 2 contained approximately 20% of the total positive signals, where each signal represented greater than or equal to 2kb of HERV sequence as determined by single copy control experiments. The highly selective integration of both complete and truncated HERV sequences into genetically active chromosome domains could indicate a preference for more structurally euchromatic sites. Retroviral sequences may initially propagate along a single chromosome, and later jump to the other active chromosome domains where they contribute to chromosome fragility and recombined with evolutionary pressures may lead to a decidedly different pattern of chromosomal organization. High resolution mapping of rodent endogenous retroviral sequences present at 800-1,000 copies per genome, combined with LINE hybridization and BrdU replication studies supported this concept. In at least one species, the Syrian hamster, endogenous retroviral sequences were entirely confined to Giemsa-dark domains that were late replicating.
Publication Types:
Keywords:
- Animals
- Base Sequence
- Chromosomes
- Chromosomes, Artificial, Yeast
- Chromosomes, Human, Pair 19
- Cricetinae
- Endogenous Retroviruses
- In Situ Hybridization
- Longitudinal Studies
- Nucleic Acid Hybridization
- Polymerase Chain Reaction
- Retroviridae
- genetics
Other ID:
UI: 102182726
From Meeting Abstracts