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Title Biomonitoring environmental contamination with pipping black-crowned night heron embryos: Induction of cytochrome P450
Creator/Author Rattner, B.A. ; Melancon, M.J. ; Custer, T.W. ; Hothem, R.L. ; King, K.A. ; LeCaptain, L.J. ; Spann, J.W. (U.S. Fish and Wildlife Service, Laurel, MD (United States). Patuxent Wildlife Research Center) ; Woodin, B.R. ; Stegeman, J.J. (Woods Hole Oceanographic Inst., MA (United States))
Publication Date1993 Sep 01
OSTI IdentifierOSTI ID: 5692428
Other Number(s)ISSN0730-7268; CODEN: ETOCDK
Resource TypeJournal Article
Resource RelationEnvironmental Toxicology and Chemistry ; Vol/Issue: 12:9
Subject560300 -- Chemicals Metabolism & Toxicology; 3-METHYLCHOLANTHRENE-- METABOLISM;BIRDS-- SENSITIVITY;MIXED-FUNCTION OXIDASES-- ENZYME INDUCTION; BIOLOGICAL MARKERS;BODY BURDEN;EMBRYOS
Related SubjectANIMALS;AROMATICS;CONDENSED AROMATICS;ENZYMES;GENE REGULATION;HYDROCARBONS;ORGANIC COMPOUNDS;OXIDOREDUCTASES;OXYGENASES;POLYCYCLIC AROMATIC HYDROCARBONS;PROTEINS;VERTEBRATES
Description/Abstract Cytochrome P450-associated monooxygenase activities and cytochrome P450 proteins were measured in pipping black-crowned night heron (Nycticorax nycticorax) embryos collected from a reference site (next to the Chincoteague National Wildlife Refuge, VA) and three polluted sites (Cat Island, Green Bay, Lake Michigan, WI; Bair Island, San Francisco Bay, CA; West Marin Island, San Francisco Bay, CA).^In a laboratory study, artificially incubated night heron embryos from the reference site were treated with 3-methylcholanthrene or phenobarbital.^Compared to controls, 3-methylcholanthrene induced a greater than fivefold increase in activities of several monooxygenases and a greater than 100-fold increase in the concentration of immunodetected cytochrome P450 1A (CYP1A).^Phenobarbital treatment resulted in only a slight increase in BROD activity but induced proteins recognized by antibodies to cytochrome P450 2B (CYP2B) by 2,000-fold.^In a field study, activities of AHH, BROD, EROD, and ethoxycoumarin-O-dealkylase (ECOD) were up to 85-fold higher in pipping black-crowned night herons collected from Cat Island compared to other sites.^Hepatic CYP1A and CYP2B cross-reactive proteins were detected in significantly more individuals from Cat Island than from the reference site.^Greatest burdens of total PCBs and p,p[prime]-DDE were detected in embryos from Cat Island.^Cytochrome P450-associated monooxygenase activities and cytochrome P450 proteins (AHH, BROD, EROD, ECOD, CYP1A, CYP1B) were significantly associated with total PCB burdens.
Country of PublicationUnited States
LanguageEnglish
FormatPages: 1719-1732
System Entry Date2001 May 13

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