Machado D, Deoliveira CF, Diaz RS, Rawal BD, Sullivan M, Gwinn M, Busch M; Conference on Retroviruses and Opportunistic Infections.

Program Abstr 6th Conf Retrovir Oppor Infect Conf Retrovir Oppor Infect 6th 1999 Chic Ill. 1999 Jan 31-Feb 4; 6th: 108 (abstract no. 216).

Blood Centers of the Pacific, San Francisco CA.

Objective: Characterize prevalence of clinically significant env and pol mutations among recently infected blood donors. Methods: Screening 5,230,463 whole blood donations from '95-'96 yielded 410 HIV infected donors (0.08%); 281 enrolled to study HIV risk factors and subtype distribution. Using a new less sensitive (LS) EIA test strategy (JAMA 1998;280:42), 34/281 (12%) were selected as recently infected (RI) and analyzed for env and pol variability. C2-V3-C3 of env, protease (PT) and 700bp of the reverse transcriptase (RT) genes were PCR-amplified and sequenced by fluorescent cycle sequencing. Results: Error-corrected sequences of env, RT and PT were obtained in 25, 31 and 29 of the 34 RI individuals. V3 sequences showed 63-94% homology to MN and SF2 isolates and to the consensus B sequence (median: 5 matches to V3 loop octameric tip). In 10/25 V3 sequences, the V3 loop tetrameric tip differed from the classic GPGR motif; in 8 the R was replaced (K=4; Q=2; G=2). 16/31 RT sequences and 19/29 PT sequences showed mutations associated with drug resistance (15 R211K; 1 K70R; and 1 V108I at the RT region; 12 L63P; 5 M36I; 5V77I; 2 L10I; and 1 M46I at the PT region). 4 cases had 2 PT mutations (3 L63I/V77I; 1 L10I/M36I), 1 had 3 mutations (M36I/M46I/L63P). Conclusions: Transmission of divergent env sequence motifs and drug-resistant mutants is occurring even among RI "low risk" blood donors, which will mean new challenges for prevention and treatment strategies in the future.

Publication Types:

• Meeting Abstracts

Keywords:

• AIDS Vaccines
• Acquired Immunodeficiency Syndrome
• Base Sequence
• Blood Donors
• Genes, env
• Genes, pol
• HIV Infections
• HIV-1
• Mutation
• Polymorphism, Genetic
• Prevalence
• genetics
• reverse transcriptase, Human immunodeficiency virus 1

Other ID:

• 20711458

UI: 102188858

From Meeting Abstracts