Tassinari P, Deibis L, Bianco N, Echeverria G; International Conference on AIDS.
Int Conf AIDS. 1992 Jul 19-24; 8: 39 (abstract no. PuA 6172).
Institute of Immunology, Central University, Venezuela.
OBJECTIVES: To evaluate CD4 subsets and response to different soluble antigens (SA) in polar stages of HIV infection. METHODS: Fourteen HIV infected asymptomatic patients (AP) and 6 AIDS (CDC, 1987), were simultaneously explored with 10 healthy heterosexual (HH) and 10 seronegative homosexuals (SN). CD3, CD4, CD29, CD45RA phenotypes were analyzed by dual-color immunofluorescence on an EPICS-753 flow cytometer. Response to optimal doses of Candida albicans (CA), SKSD and tetanus toxoid (TT) was explored in PBL, enriched CD4 and CD4CD29 (over 95%) populations by cell culture and 3HT incorporation. Results were analyzed by students t-test and Pearson correlation coefficient. RESULTS: Significant depletion of CD4 and both memory (CD29+) and naive (CD45RA) was found among AP and AIDS when compared to control groups. Response to SA in AP is diminished in PBL, statistically significant to CA (15.308 vs 35.951) but not to TT(30.413 vs 78.512) or SKSD (10.985 vs 15.751). Diminished response to CA is more evident when CD4CD29 enriched cells are studied, and failure to respond to SKSD is also evidenced (3.048 vs 10.367). The AP group comprised 7 responders (R) to at least one SA and 7 non-responders (NR); both CD29+ and CD45RA absolute counts although diminished when compared to controls, were similar among R and NR (133 vs 115 and 142 vs 109). CONCLUSIONS: 1) half of our AP exhibited a qualitative defect of CD4CD29 response to environmental antigens (CA and SKSD). 2) defective response was unrelated to CD4CD29 absolute numbers. 3) our results suggest that defective response to environmental antigens is an early event in HIV infection. 4) preparations enriched in memory cells appear to be needed for early observation of unresponsiveness to environmental antigens.
Publication Types:
Keywords:
- Acquired Immunodeficiency Syndrome
- Antigens
- Antigens, CD29
- Antigens, CD3
- Antigens, CD4
- Antigens, CD45
- HIV Infections
- Homosexuality
- Humans
- Tetanus Toxoid
- immunology
Other ID:
UI: 102201967
From Meeting Abstracts