All you probably have to do in order to run this example is to click "Submit Query" at the bottom of this page. If you want to run your own set of sequences or you want to customize the output, enter values into the sections one to four of the form below. The email address (section 1) is only needed for alignments with a length of more than 100 nucleotides. Sections 3 and 4 are optional. For more detailed information consult our help page.
Background of example sequences:
The sequences provided in this example correspond to the tRNA. The alignment is derived from the Rfam entry of tRNA. In order to obtain a simple example, the original Rfam seed alignment with 1114 sequence was modified: the alignment was "collapsed" with respect to the Saccharomyces cerevisiae PHE-tRNA sequence (K01553.1) (this means that all columns corresponding to a gap in that sequence were removed). Please note that this example is "large": the default parameters work fine for the systems we tested, but if you specify a lower cutoff below 0.1 bits, you might experience problems with both JVX and VRML due to browser/viewer limitations with respect to the maximum number of displayable polygons. In this case it helps to reduce the number of polygons by using a smart choice of options (no 3D logo characters, no 2D sequence logo, option "Optimize for 3D printing" activated). MS Internet Explorer was in our experience the most "resilient" when it comes to displaying large models with the used applet. Another possibility is to download the generated JVX or VRML files and view them with standalone programs (like the JavaView standalone application).
Useful Links:
Link to the Rfam entry of tRNA
For an explanation of the generated output and help converning VRML and JVX please see our help page.
If you want to pick up a previously computed result, click here.
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