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BLASTX+BEAUTY Search Results

WU-BLAST 2.0 search of the National Center for Biotechnology Information's NR Protein Database.

BEAUTY post-processing provided by the Human Genome Sequencing Center, Baylor College of Medicine.

BEAUTY Reference:
Worley KC, Culpepper P, Wiese BA, Smith RF. BEAUTY-X: enhanced BLAST searches for DNA queries. Bioinformatics 1998;14(10):890-1. Abstract

Worley KC, Wiese BA, Smith RF. BEAUTY: an enhanced BLAST-based search tool that integrates multiple biological information resources into sequence similarity search results. Genome Res 1995 Sep;5(2):173-84 Abstract



RepeatMasker repeats found in sequence:

No Repeats Found.

Reference:  Gish, Warren (1994-1997).  unpublished.
Gish, Warren and David J. States (1993).  Identification of protein coding
regions by database similarity search.  Nat. Genet. 3:266-72.

Notice: statistical significance is estimated under the assumption that the equivalent of one entire reading frame in the query sequence codes for protein and that significant alignments will involve only coding reading frames.

Query= A10G10_CONSENSUS (498 letters)

  Translating both strands of query sequence in all 6 reading frames

Database: nr 625,274 sequences; 197,782,623 total letters.



     Observed Numbers of Database Sequences Satisfying
    Various EXPECTation Thresholds (E parameter values)

        Histogram units:      = 5 Sequences     : less than 5 sequences

 EXPECTation Threshold
 (E parameter)
    |
    V   Observed Counts-->
  10000 761 264 |====================================================
   6310 497 129 |=========================
   3980 368 149 |=============================
   2510 219  75 |===============
   1580 144  56 |===========
   1000  88  26 |=====
    631  62  22 |====
    398  40   8 |=
    251  32   9 |=
    158  23   3 |:
    100  20   1 |:
   63.1  19   2 |:
   39.8  17   1 |:
   25.1  16   4 |:
   15.8  12   2 |:
 >>>>>>>>>>>>>>>>>>>>>  Expect = 10.0, Observed = 10  <<<<<<<<<<<<<<<<<
   10.0  10   0 |
   6.31  10   0 |
   3.98  10   1 |:
   2.51   9   1 |:
   1.58   8   1 |:
   1.00   7   0 |
   0.63   7   1 |:
   0.40   6   0 |
   0.25   6   0 |
   0.16   6   2 |:
   0.10   4   0 |
  0.063   4   0 |
  0.040   4   0 |
  0.025   4   1 |:
  0.016   3   0 |
  0.010   3   1 |:


                                                                     Smallest
                                                                       Sum
                                                     Reading  High  Probability
Sequences producing High-scoring Segment Pairs:        Frame Score  P(N)      N
gi|9279667|dbj|BAB01224.1|(AB016889) gene_id:MDJ14.7~... +1   353  2.9e-31   1
gi|8698732|gb|AAF78490.1|AC012187_10(AC012187) Contai... +1   342  1.3e-29   1
gi|7451674|pir||S76530hypothetical protein sll0395 - ... +1    99  0.0066    1
gi|7473626|pir||A75439probable phosphoglycerate mutas... +1    96  0.024     1
gi|11761356|dbj|BAB19263.1|(AB052566) phosphoglycerat... +1    94  0.12      1
gi|7451673|pir||S77391hypothetical protein slr1748 - ... +1    90  0.14      1
gi|11362518|pir||F82627phosphoglycerate mutase XF1886... +1    86  0.39      1
gi|548532|sp|P36623|PMGY_SCHPOPHOSPHOGLYCERATE MUTASE... +1    83  0.69      1
gi|11349154|pir||F83486hypothetical protein PA1280 [i... +1    81  0.83      1
gi|2135627|pir||I63131metallothionein old gene name '... -3    63  0.94      1



Locally-aligned regions (HSPs) with respect to query sequence:

Locus_ID                Frame 1 Hits
gi|9279667             | __________________________________               
gi|8698732             |_______________________________________           
gi|7451674             |  _______________________                         
gi|7473626             |___________________________                       
gi|11761356            |  _________________________                       
gi|7451673             |   ______________________                         
gi|11362518            |    _______________________                       
gi|548532              |____________________________________              
gi|11349154            | __________________________                       
                        __________________________________________________
Query sequence:        |              |              |              |     | 166
                       0             50            100            150


Locus_ID                Frame -3 Hits
gi|2135627             |__________                                        
                        __________________________________________________
Query sequence:        |              |              |              |     | 166
                       0             50            100            150

Use the and icons to retrieve links to Entrez:

E = Retrieve Entrez links (e.g., Medline abstracts, FASTA-formatted sequence reports).
R = Retrieve links to Related sequences (neighbors).
Use the icon (if present) to retrieve links to the Sequence Retrieval System (SRS).
Use the icon (if present) to retrieve links to the Ligand Enzyme and Chemical Compound Database .
Use the icon (if present) to retrieve links to the Protein Data Bank database.


to_Entrezto_Relatedto_Related >gi|9279667|dbj|BAB01224.1|  (AB016889) gene_id:MDJ14.7~similar to unknown
            protein~sp|P07953 [Arabidopsis thaliana]
            Length = 399

Frame  1 hits (HSPs):                      _______________                
                        __________________________________________________
Database sequence:     |                  |                  |            | 399
                       0                150                300

  Plus Strand HSPs:

 Score = 353 (124.3 bits), Expect = 2.9e-31, P = 2.9e-31
 Identities = 72/113 (63%), Positives = 85/113 (75%), Frame = +1

Query:    13 KSQGVRFXAVYSSPLDRARSTAVSVCKEVNFSXEQIQSTDALAEISQGNWEGCLXSXIYT 192
             KSQGVRF +VYSSPLDRARS AVSVC+E++F  E +QS+DA+ E+S G+WEGC  + IY+
Sbjct:   154 KSQGVRFNSVYSSPLDRARSMAVSVCQEMSFPEEHVQSSDAVIEMSLGDWEGCNQAEIYS 213

Query:   193 PEIQGLIDRFXPDFTAPSGESLRQVXFXMIHFLNGTVXAXHXKLL-DLSSHQN 348
             PE   LI+R  PDFTAPSGESLRQV F M+ FLNGTV     KL  D SS  N
Sbjct:   214 PETLSLIERCQPDFTAPSGESLRQVEFRMVQFLNGTVSGVAEKLRSDYSSTTN 266


to_Entrezto_Relatedto_Related >gi|8698732|gb|AAF78490.1|AC012187_10  (AC012187) Contains weak similarity to
            LIP1 gene product gi|7297743 from Drosophila melanogaster genomic
            sequence gb|AE003629 and is a member of the Phosphoglycerate mutase
            PF|00300 family.  ESTs gb|N38556, gb|Z25988, gb|Z18409 come from
            this gene. [Ar>
            Length = 567

Frame  1 hits (HSPs):                 ____________                        
                        __________________________________________________
Database sequence:     |             |            |            |          | 567
                       0           150          300          450

  Plus Strand HSPs:

 Score = 342 (120.4 bits), Expect = 1.3e-29, P = 1.3e-29
 Identities = 73/130 (56%), Positives = 89/130 (68%), Frame = +1

Query:    10 LKSQGVRFXAVYSSPLDRARSTAVSVCKEVNFSXEQIQSTDALAEISQGNWEGCLXSXIY 189
             L S+GVRF +V+SSPLDRARS AVSVC+E+NF  E +Q +DA+ E+S G+WE C  S IY
Sbjct:   164 LNSEGVRFTSVFSSPLDRARSMAVSVCQEMNFPEEHLQVSDAVVEMSLGDWESCHRSEIY 223

Query:   190 TPEIQGLIDRFXPDFTAPSGESLRQVXFXMIHFLNGTVXAXHXKL----LDLSSHQNDXQ 357
             TPE   LI+R  PDF+APSGESLRQV F M+ FLNGTV     KL    L  + H N  +
Sbjct:   224 TPETLSLIERCQPDFSAPSGESLRQVEFRMVQFLNGTVSGLSEKLRSELLPSTQHTNS-R 282

Query:   358 AFAQNXS-HAP 387
              F+   S H P
Sbjct:   283 GFSLATSIHRP 293


to_Entrezto_Relatedto_Related >gi|7451674|pir||S76530  hypothetical protein sll0395 - Synechocystis sp.
            (strain PCC 6803) >gi|1001645|dbj|BAA10376.1| (D64002) hypothetical
            protein [Synechocystis sp.]
            Length = 212

Frame  1 hits (HSPs):             __________________                      
                        __________________________________________________
Database sequence:     |           |           |           |          |   | 212
                       0          50         100         150        200

  Plus Strand HSPs:

 Score = 99 (34.8 bits), Expect = 0.0066, P = 0.0066
 Identities = 27/75 (36%), Positives = 41/75 (54%), Frame = +1

Query:    25 VRFXAVYSSPLDRARSTAVSVCKEVNFSXEQIQSTDALAEISQGNWEGCLXSXI----YT 192
             V + A+++SPL R  +TA  + K +N     IQ  D L EI+ G WEG   + +    + 
Sbjct:    47 VSWTAIFASPLHRTMATATPLSKLINLP---IQKRDGLKEIAYGEWEGKTPAEVNQQFHD 103

Query:   193 PEIQGLIDRFXPDFTAPSG 249
               ++ L D   P + APSG
Sbjct:   104 DYVRWLAD---PGWNAPSG 119


to_Entrezto_Relatedto_Related >gi|7473626|pir||A75439  probable phosphoglycerate mutase - Deinococcus
            radiodurans  (strain R1) >gi|6458827|gb|AAF10667.1|AE001959_7
            (AE001959) phosphoglycerate mutase, putative [Deinococcus
            radiodurans]
            Length = 232

Frame  1 hits (HSPs):                ___________________                  
                        __________________________________________________
Database sequence:     |          |          |          |         |       | 232
                       0         50        100        150       200

  Plus Strand HSPs:

 Score = 96 (33.8 bits), Expect = 0.024, P = 0.024
 Identities = 28/86 (32%), Positives = 46/86 (53%), Frame = +1

Query:    10 LKSQGVRFXAVYSSPLDRARSTAVSVCKEVNFSXEQIQSTDALAEISQGNWEGCLXSXIY 189
             L+  GV+   +++S L RA +TA ++ +E+  +   + +   L EIS G+WEG L   I 
Sbjct:    62 LRRLGVQAPRIHASDLSRAHATAEALHRELGGT---LATFPELREISLGDWEGHLYDEIA 118

Query:   190 T--PEIQGLIDRFXPDFTAPSGESLRQV 267
                PE+ G      P+   P GE+ + V
Sbjct:   119 ARHPELHGQFWSGDPECCPPGGETPQAV 146


to_Entrezto_Relatedto_Related >gi|11761356|dbj|BAB19263.1|  (AB052566) phosphoglycerate mutase
            [Thermosynechococcus elongatus]
            Length = 414

Frame  1 hits (HSPs):                               ___________           
                        __________________________________________________
Database sequence:     |                 |                  |             | 414
                       0               150                300

  Plus Strand HSPs:

 Score = 94 (33.1 bits), Expect = 0.13, P = 0.12
 Identities = 31/81 (38%), Positives = 47/81 (58%), Frame = +1

Query:    25 VRFXAVYSSPLDRARSTAVSVCKEVNFSXEQIQSTDALAEISQGNWEGCLXSXIYTPEIQ 204
             V F    SSPL R + TA+++ +       Q++   ALAEIS G+WEG      + PE++
Sbjct:   240 VPFHHAVSSPLLRPKDTALAILQY--HPHVQLELEPALAEISHGDWEGK-----FEPEVE 292

Query:   205 ----GLIDRF--XPDFTA-PSGESLRQV 267
                 G ++R+   P     P+GE+L QV
Sbjct:   293 AAYPGELERWRTTPALVQMPNGENLEQV 320


to_Entrezto_Relatedto_Related >gi|7451673|pir||S77391  hypothetical protein slr1748 - Synechocystis sp.
            (strain PCC 6803) >gi|1652573|dbj|BAA17494.1| (D90906) hypothetical
            protein [Synechocystis sp.]
            Length = 214

Frame  1 hits (HSPs):               _________________                     
                        __________________________________________________
Database sequence:     |           |           |          |           |   | 214
                       0          50         100        150         200

  Plus Strand HSPs:

 Score = 90 (31.7 bits), Expect = 0.15, P = 0.14
 Identities = 25/71 (35%), Positives = 36/71 (50%), Frame = +1

Query:    37 AVYSSPLDRARSTAVSVCKEVNFSXEQIQSTDALAEISQGNWEGCLXSXIYTPE----IQ 204
             AVY SPL RAR T   +C  +     ++Q    L E++ G WEG     +Y  +    +Q
Sbjct:    53 AVYVSPLQRARQTVQPLCDRLGI---EMQIRPGLREVAYGEWEGLHPDEVYQRDHDLYMQ 109

Query:   205 GLIDRFXPDFTAPSG 249
              L D   P + +P G
Sbjct:   110 WLTD---PAWNSPPG 121


to_Entrezto_Relatedto_Related >gi|11362518|pir||F82627  phosphoglycerate mutase XF1886 [imported] - Xylella
            fastidiosa (strain 9a5c) >gi|9106977|gb|AAF84692.1|AE004008_16
            (AE004008) phosphoglycerate mutase [Xylella fastidiosa]
            Length = 214

Frame  1 hits (HSPs):              __________________                     
                        __________________________________________________
Database sequence:     |           |           |          |           |   | 214
                       0          50         100        150         200

  Plus Strand HSPs:

 Score = 86 (30.3 bits), Expect = 0.50, P = 0.39
 Identities = 29/74 (39%), Positives = 40/74 (54%), Frame = +1

Query:    46 SSPLDRARSTAVSVCKEVNFSXEQIQSTDALAEISQGNWEGCLXSXIYTPEIQGLID-RF 222
             +SPL RA+ TA     E   +   I++   L EIS G+WEG L + I+  +   L   R 
Sbjct:    52 ASPLVRAQYTAQLALGESRAAQLLIEAD--LKEISHGDWEGLLDTEIHAMDPTRLHAWRK 109

Query:   223 XPDFTA-PSGESLRQV 267
              P+    P+GESL QV
Sbjct:   110 APETVIMPNGESLHQV 125


to_Entrezto_Relatedto_Relatedto_ec >gi|548532|sp|P36623|PMGY_SCHPO  PHOSPHOGLYCERATE MUTASE (PHOSPHOGLYCEROMUTASE)
            (PGAM) (MPGM) (BPG-DEPENDENT PGAM) >gi|539074|pir||S43214
            phosphoglycerate mutase (EC 5.4.2.1) - fission yeast
            (Schizosaccharomyces pombe) >gi|629902|pir||S43369 phosphoglycerate
            mutase (EC 5.4.2.1) - fission yeast (Schizosaccharomyces pombe)
            >gi|407527|emb|CAA53154.1| (X75385) phosphoglycerate mutase
            [Schizosaccharomyces pombe] >gi|1314175|emb|CAA97363.1| (Z73100)
            phosphoglycerate mutase (EC 5.4.2.1) [Schizosaccharomyces pombe]
            Length = 211

Frame  1 hits (HSPs):              _____________________________          
Annotated Domains:      __________________________________________________
                        __________________________________________________
Database sequence:     |           |           |           |           |  | 211
                       0          50         100         150         200
__________________

Annotated Domains:
   BLOCKS               BL00175A: Phosphoglycerate mutase family 10..29
   BLOCKS               BL00175B: Phosphoglycerate mutase family 59..71
   BLOCKS               BL00175C: Phosphoglycerate mutase family 83..114
   BLOCKS               BL00175D: Phosphoglycerate mutase family 139..190
   DOMO                 DM00691: PHOSPHOGLYCERATEMUTASEFAMILYPHO 6..210
   Entrez               active site: FORMS THE PHOSPHOHISTIDINE  15
   Entrez               active site: REQUIRED FOR BINDING CARBOX 66
   Entrez               active site: BY SIMILARITY.              163
   PFAM                 PGAM: Phosphoglycerate mutase family     11..200
   PRODOM               PD000730: PMGY(6) PMGM(3) PMGE(3)        3..96
   PRODOM               PD003480: PMGY(7) PMGM(3) PMGE(3)        99..208
   PROSITE              PG_MUTASE: Phosphoglycerate mutase famil 12..21
__________________


  Plus Strand HSPs:

 Score = 83 (29.2 bits), Expect = 1.2, P = 0.69
 Identities = 34/121 (28%), Positives = 58/121 (47%), Frame = +1

Query:    10 LKSQGVRFXAVYSSPLDRARSTAVSVCKEVNF-SXEQIQSTDALAEISQGNWEGCLXSXI 186
             LKS+G +F   ++S L RA+ T   + +EV   + E I+S + L E   G+ +G      
Sbjct:    49 LKSRGYKFDIAFTSALQRAQKTCQIILEEVGEPNLETIKS-EKLNERYYGDLQGLNKDDA 107

Query:   187 ---YTPEIQGLIDRFXPDFTAPSGESLRQVXFXMIHFLNGTVXAXHXK--LLDLSSHQND 351
                +  E Q  I R   D   P+GESL+     ++ +   T+     K   + +++H N 
Sbjct:   108 RKKWGAE-QVQIWRRSYDIAPPNGESLKDTAERVLPYYKSTIVPHILKGEKVLIAAHGNS 166

Query:   352 XQA 360
              +A
Sbjct:   167 LRA 169


to_Entrezto_Relatedto_Related >gi|11349154|pir||F83486  hypothetical protein PA1280 [imported] - Pseudomonas
            aeruginosa (strain PAO1) >gi|9947214|gb|AAG04669.1|AE004557_10
            (AE004557) hypothetical protein [Pseudomonas aeruginosa]
            Length = 194

Frame  1 hits (HSPs):             _____________________                   
                        __________________________________________________
Database sequence:     |            |            |            |           | 194
                       0           50          100          150

  Plus Strand HSPs:

 Score = 81 (28.5 bits), Expect = 1.8, P = 0.83
 Identities = 25/82 (30%), Positives = 40/82 (48%), Frame = +1

Query:    19 QGVRFXAVYSSPLDRARSTAVSVCKEVNFSXEQIQSTDALAEISQGNWEGCLXSXIYTPE 198
             +G R+  + SSPL R R+ A  + +       +++  + L E+  G WEG   + +    
Sbjct:    40 EGGRWDLLVSSPLQRCRAFAEELAQRQGI---ELELENDLRELHFGAWEGRSAAALMDGH 96

Query:   199 IQGLIDRFXPD---FTAPSGESLRQ 264
              + L  RF  D   FT P GE L +
Sbjct:    97 SEAL-GRFWADPYAFTPPGGEPLSE 120


to_Entrezto_Relatedto_Related >gi|2135627|pir||I63131  metallothionein old gene name 'MT2' - human (fragment)
            >gi|187528|gb|AAA59584.1| (M26637) metallothionein old gene name
            'MT2' [Homo sapiens]
            Length = 66

Frame -3 hits (HSPs):     ________________________                        
                        __________________________________________________
Database sequence:     |              |              |              |     | 66
                       0             20             40             60

  Minus Strand HSPs:

 Score = 63 (22.2 bits), Expect = 2.9, P = 0.94
 Identities = 15/32 (46%), Positives = 17/32 (53%), Frame = -3

Query:    97 LPCTPTPPWT-APDPAGTNTRR*TEPPAT*AA 5
             +P  P PP T AP PA  N +    PPA  AA
Sbjct:     4 IPTAPAPPVTPAPAPAPANAKSANAPPARKAA 35


Parameters:
  filter=none
  matrix=BLOSUM62
  V=50
  B=50
  E=10
  gi
  H=1
  sort_by_pvalue
  echofilter

  ctxfactor=5.91

  Query                        -----  As Used  -----    -----  Computed  ----
  Frame  MatID Matrix name     Lambda    K       H      Lambda    K       H
   Std.    0   BLOSUM62                                 0.318   0.135   0.401  
   +3      0   BLOSUM62        0.318   0.135   0.401    0.339   0.150   0.542  
               Q=9,R=2         0.244   0.0300  0.180     n/a     n/a     n/a
   +2      0   BLOSUM62        0.318   0.135   0.401    0.346   0.153   0.528  
               Q=9,R=2         0.244   0.0300  0.180     n/a     n/a     n/a
   +1      0   BLOSUM62        0.317   0.132   0.385    same    same    same
               Q=9,R=2         0.244   0.0300  0.180     n/a     n/a     n/a
   -1      0   BLOSUM62        0.318   0.135   0.401    0.360   0.167   0.632  
               Q=9,R=2         0.244   0.0300  0.180     n/a     n/a     n/a
   -2      0   BLOSUM62        0.318   0.135   0.401    0.335   0.144   0.503  
               Q=9,R=2         0.244   0.0300  0.180     n/a     n/a     n/a
   -3      0   BLOSUM62        0.318   0.135   0.401    0.338   0.151   0.505  
               Q=9,R=2         0.244   0.0300  0.180     n/a     n/a     n/a

  Query
  Frame  MatID  Length  Eff.Length     E    S W   T  X   E2     S2
   +3      0      165       149       10.  74 3  12 22  0.092   34
                                                    30  0.12    36
   +2      0      165       146       10.  74 3  12 22  0.12    33
                                                    30  0.11    36
   +1      0      166       147       10.  74 3  12 22  0.12    33
                                                    30  0.11    36
   -1      0      166       147       10.  74 3  12 22  0.12    33
                                                    30  0.11    36
   -2      0      165       146       10.  74 3  12 22  0.12    33
                                                    30  0.11    36
   -3      0      165       148       10.  74 3  12 22  0.091   34
                                                    30  0.11    36


Statistics:

  Database:  /usr/local/dot5/sl_home/beauty/seqdb/blast/nr
    Title:  nr
    Release date:  unknown
    Posted date:  4:06 PM CST Feb 28, 2001
    Format:  BLAST
  # of letters in database:  197,782,623
  # of sequences in database:  625,274
  # of database sequences satisfying E:  10
  No. of states in DFA:  593 (58 KB)
  Total size of DFA:  174 KB (192 KB)
  Time to generate neighborhood:  0.01u 0.00s 0.01t  Elapsed: 00:00:00
  No. of threads or processors used:  6
  Search cpu time:  145.01u 1.17s 146.18t  Elapsed: 00:00:25
  Total cpu time:  145.04u 1.19s 146.23t  Elapsed: 00:00:25
  Start:  Mon Oct  1 23:13:33 2001   End:  Mon Oct  1 23:13:58 2001

Annotated Domains Database: March 14, 2000
Release Date: March 14, 2000