Genetic analysis of succinate utilization in enzyme I mutants of the phosphoenolpyruvate: sugar phosphotransferase system in Escherichia coli.

J Bacteriol. 1975 October; 124(1): 252–261.

PMCID: PMC235890

J K Alexander and B Tyler

This article has been cited by other articles in PMC.

Abstract

Studies on the reversion characteristics of Escherichia coli strains carrying various mutations in the pts region have led to the recognition of a mutation, suc-1, with a previously undescribed phenotype. Strains carrying the suc-1 mutation grow normally on most sources of carbon but are unable to utilize succinate effectively. The suc-1 mutation can be separated genetically from the tightly linked ptsI6 mutation. Reversion of suc-1 mutants for growth on succinate yields interesting classes of suppressor mutations.

Full text

Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (1.4M), or click on a page image below to browse page by page. Links to PubMed are also available for Selected References.

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

Berman, M; Zwaig, N; Lin, EC. Suppression of a pleiotropic mutant affecting glycerol dissimilation. Biochem Biophys Res Commun. 1970 Jan 23;38(2):272–278. [PubMed]
Dahl, R; Morse, HG; Morse, ML. Carbohydrate transport and cyclic 3',5' adenosine monophosphate (cAMP) levels in a temperature sensitive phosphotransferase mutant of Escherichia coli. Mol Gen Genet. 1974 Mar 6;129(1):1–10. [PubMed]
Epstein, W; Jewett, S; Fox, CF. Isolation and mapping of phosphotransferase mutants in Escherichia coli. J Bacteriol. 1970 Nov;104(2):793–797. [PubMed]
Kennedy, CK. Induction of colicin production by high temperature or inhibition of protein synthesis. J Bacteriol. 1971 Oct;108(1):10–19. [PubMed]
Lo, TC; Rayman, MK; Sanwal, BD. Transport of succinate in Escherichia coli. I. Biochemical and genetic studies of transport in whole cells. J Biol Chem. 1972 Oct 10;247(19):6323–6331. [PubMed]
Pastan, I; Perlman, RL. Repression of beta-galactosidase synthesis by glucose in phosphotransferase mutants of Escherichia coli. Repression in the absence of glucose phosphorylation. J Biol Chem. 1969 Nov 10;244(21):5836–5842. [PubMed]
Saier, MH; Roseman, S. Inducer exclusion and repression of enzyme synthesis in mutants of Salmonella typhimurium defective in enzyme I of the phosphoenolpyruvate: sugar phosphotransferase system. J Biol Chem. 1972 Feb 10;247(3):972–975. [PubMed]
Tanaka, S; Fraenkel, DG; Lin, EC. The enzymatic lesion of strain MM-6, a pleiotropic carbohydrate-negative mutant of Escherichia coli. Biochem Biophys Res Commun. 1967 Apr 7;27(1):63–67. [PubMed]
Tyler, B; Wishnow, R; Loomis, WF, Jr; Magasanik, B. Catabolite repression gene of Escherichia coli. J Bacteriol. 1969 Nov;100(2):809–816. [PubMed]
Wang, Richard J; Morse, Helvise G; Morse, ML. Carbohydrate Accumulation and Metabolism in Escherichia coli: Characteristics of the Reversions of ctr Mutations. J Bacteriol. 1970 Dec;104(3):1318–1324. [PubMed]