Lucy Stols

Publications:

1. Donnelly, M.I.,  Zhou, M., Millard, C.S., Clancy, S., Stols,  L., Eschenfeldt, W.E., Collart, F.R., and Joachimiak, A.,(2006) An expression vector tailored for large-scale, high-throughput purification of recombinant proteins. Protein Expr. Purif. In press.
2. Eirich, L. D., Craft, D.L., Steinberg, L., Asif, A., Eschenfeldt, W.E., Stols,  L.,.
   Donnelly, M.I., and Wilson, C.R. (2004) Cloning and Characterization of Three Fatty Alcohol Oxidase Genes from Candida tropicalis Strain ATCC 20336. Appl. Environ. Micro.70, 4872-4879.
3. Stols, L., Sanville Millard, C., Dementieva, I. and Donnelly, M.I. (2004)
   Production of selenomethionine-labeled proteins in two-liter plastic bottles for structure determination. J. Struct. Funct. Genomics 5, 95-102.
4. Millard, C.S., Stols, L., Quartey, P., Kim, Y., Demetieva, I., and Donnelly, M.I.  (2003) A less laborious approach to the high-throughput production of recombinant proteins in Escherichia coli using 2-liter plastic bottles Prot. Express. Purif. 29, 311-320.
5. Eschenfeldt, W. H., Zhang, Y., Samaha, H., Stols, L., Eirich,  L.D., Wilson, C.R., and . Donnelly, M.I. (2003) Transformations of Fatty Acids Catalyzed by Cytochrome P450 Monooxygenase Enzymes of Candida tropicalis. Appl. Environ. Microbiol. 69, 5992-5999.
6. Stols, L., Gu, M., Dieckman, L., Raffen, R., Collart, R.F., and Donnelly, M.I. (2002) A New Vector for High Throughput, Ligation Independent Cloning Encoding a TEV Protease Cleavage Site. Prot. Expr. Purif. 25, 8-15.
7. Dieckman, L., Gu, M., Stols, L.,Donnelly, M.I., and Collart, F.R (2002) High Throughput Methods for Gene Cloning and Expression, Prot. Expr. Purif. 25, 1-7.
8. Eschenfeldt, W.H., Stols, L., Rosenbaum, H., Khambatta, Z.S., Quaite-Randall, E.,.
   Wu, S., Kilgore, D.C.,  Trent, J.D., and Donnelly, M.I. (2001) DNA from Uncultured Organisms as a Source of 2,5-Diketo-D-Gluconic Acid Reductases. Appl. Environ. Microbiol. 67, 4206-4214.
9. Donnelly, M.I., Wilkins Stevens, P., Stols, L., Su, S.X., Tollaksen, S..Giometti, C.S., and Joachimiak, A. (2001) Expression of a Highly Toxic Protein, Bax, in Escherichia coli by Attachment of a Leader Peptide Derived from the GroES Co-chaperone. Protein Expr. Purif. 22, 422-429.
10. Stols, L. and Donnelly, M.I. (1997) Production of succinic acid through overexpression of NAD+ dependent malic enzyme in an Escherichia coli  mutant. Appl. Environ. Microbiol.  63, 2695-2701.
11. Stols, L., Kulkarni, G., Harris, B.G., and Donnelly, M.I. (1997) Expression of Ascaris suum malic enzyme in a mutant Escherichia coli allows production of succinic acid from glucose. Appl. Biochem. Biotechnol. 63-65, 153-158.
12. Morrison, L.E., Stols, L.M. (1993) Sensitive fluorescence-based thermodynamic and kinetic measurements of DNA hybridization in solution.  Biochemistry 32(12): 3095-104.
13. Morrison, L.E., Halder, T.C.,  Stols, L. (1989) Solution-phase detection of polynucleotides using interacting fluorescent labels and competitive hybridization. Anal.Biochem  183(2): 231-44.
14. Stols, L., Iannaccone, P.M. (1985)  Pattern of steroid interaction with inducers of aryl hydrocarbon hydroxylase activity in several cloned estradiol-respnsive stromal cell strains from mouse endometrium.  J. Cell Physiol.. 123(3): 395-400.
15. Iannaccone, P.M., Fahl, W.E.,  Stols, L. (1984)  Reproductive toxicity associated with endometrial cell mediated metabolism of benzo[a]pyrene;  a combined in vitro, in vivo approach.  Carcinogenesis 5(11):1437-42.
16. Iannaccone, P.M. Stols, L. Hollenberg, P.F. Gurka, D.P.  An estradiol-responsive mouse endometrial cell strain with inducible aryl hydrocarbon hydroxylase activity.  J Cell Physiol. 116(2): 227-35.
17. Iannaccone, P.M., Tsao, T.Y., Stols. L.  Effects on mouse blastocysts of in vitro exposure to methylnitrosourea and 3-methylcholanthrene.  Cancer Res. 42(3): 864-8.

Patents:

• Ngheim, N.P., Donnelly, M.I., Millard, C.S., and Stols, L. Method for the production of dicarboxylic acids. USPTO Patent 5,869,301, Feb. 9, 1999.
• Donnelly, M.I., Millard, C.S., and Stols, L. Mutant Escherichia coli strain with increased production of succinic acid. USPTO Patent 5,770,435, Jun. 23, 1998.
• Bittner, M. L., Stols, L.M., Prorok, C.F.,  Cruickshank, K. A., Production of chromosome region specific DNA sequences and transamination. USPTO Patent 5,506,350,  April 9, 1996.