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Title Incorporation of UDPglucose into cell wall glucans and lipids by intact cotton fibers
Creator/Author Dugger, W.M. ; Palmer, R.L.
Publication Date1986 Jun 01
OSTI IdentifierOSTI ID: 7188090
Other Number(s)CODEN: PLPHA
Resource TypeJournal Article
Resource RelationPlant Physiol. ; Vol/Issue: 81:2
Research OrgUniv. of California, Riverside
Subject551001 -- Physiological Systems-- Tracer Techniques; COTTON PLANTS-- PHYSIOLOGY;GLUCOSE-- BIOCHEMICAL REACTION KINETICS; BIOSYNTHESIS;CARBON 14 COMPOUNDS;CELL MEMBRANES;COTTON;ENZYME ACTIVITY;FIBERS;LIPIDS;POLYSACCHARIDES;TRACER TECHNIQUES;TRITIUM COMPOUNDS
Related SubjectALDEHYDES;CARBOHYDRATES;CELL CONSTITUENTS;HEXOSES;ISOTOPE APPLICATIONS;KINETICS;LABELLED COMPOUNDS;MEMBRANES;MONOSACCHARIDES;ORGANIC COMPOUNDS;PLANTS;REACTION KINETICS;SACCHARIDES;SYNTHESIS
Description/Abstract The (/sup 14/C) moiety from (/sup 3/H)UDP(/sup 14/C)glucose was incorporated by intact cotton fibers into hot water soluble, acetic-nitric reagent soluble and insoluble components, and chloroform-methanol soluble lipids; the (/sup 3/H)UDP moiety was not incorporated.^The /sup 3/H-label can be exchanged rapidly with unlabeled substrate in a chase experiment.^The cell wall apparent free space of cotton fibers was in the order of 30 picomoles per milligram of dry fibers; 25 picomoles per milligram easily exchanged and about 5 picomoles per milligram more tightly adsorbed.^At 50 micromolar UDPglucose, 70% of the (/sup 14/C)glucose was found in the lipid fraction after both a short labeling period and chase.^The percent of (/sup 14/C)glucose incorporated into total glucan increased within a 30-minute chase period.^The data supports the concept that glucan synthesis, including cellulose, as well as the synthesis of steryl glucosides, acetylated steryl glucosides, and glucosyl-phosphoryl-polyprenol from externally supplied UDPglucose occurs at the plasma membrane-cell wall interface.^The synthase enzymes for such synthesis must be part of this interfacial membrane system.
Country of PublicationUnited States
LanguageEnglish
FormatPages: 464-470
System Entry Date2001 May 13

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