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Title Comparing models for vesicant responses in skin cells
Creator/Author Mershon, M.M. ; Rhoads, L.S. ; Petrali, J.P. ; Mills, K.R. ; Kim, S.K.
Publication Date1993 May 13
OSTI IdentifierOSTI ID: 7239952
Report Number(s)AD-P-008759/3/XAB
Resource TypeTechnical Report
Resource RelationThis article is from `Proceedings of the Medical Defense Bioscience Review (1993) Held in Baltimore, Maryland on 10-13 May 1993. Volume 1`, AD-A275 667, p67-76
Research OrgArmy Medical Research Inst. of Chemical Defense, Aberdeen Proving Ground, MD (United States)
Subject450600 -- Military Technology, Weaponry, & National Defense-- Chemical & Biological-- (1990) ;560300 -- Chemicals Metabolism & Toxicology; CHEMICAL WARFARE AGENTS-- TOXICITY
Related SubjectWEAPONS
Description/Abstract Vesicant challenges have been delivered to NHEK (normal human epidermal keratinocyes) and to artificial human epidermal tissues.^Confluent NHEK, grown on plastic surfaces or gel-coated microporous membranes of Millicell CMR inserts, were challenged with vesicants diluted in cell culture medium.^Testskin was provided on agarose nutrient gel as a cornified wafer of sufficient diameter to receive vesicant vapor from cups normally used to challenge animal skin.^Stratum corneum of preproduction EpiDerm (PreEpiD) specimens were challenged with vesicant vapor from cups suspended inside of Millicells.^Inverted phase contrast microscopy of NHEK on plastic revealed dose-related vesicant effects that could facilitate screening of antivesicants.^Scanning electron microscopy (SEM) revealed vesicant effects in two distinctly different populations of NHEK on gel-coated inserts.^SEM and transmission electron microscopy (TEM) of Testskin and PreEpiD disclosed structural differences between these models that became amplified in vesicant-challenged specimens.^PreEpiD shows more promise than Testskin for screening of antivesicant topical skin protectants.^However, both epidermal models lack the basal lamina that is needed for advanced antivesicant testing.
Country of PublicationUnited States
LanguageEnglish
FormatPages: (10 p)
AvailabilityNTIS
System Entry Date2001 May 13

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