Bibliographic Citation
| Document | For copies of Journal Articles, please contact the Publisher or your local public or university library and refer to the information in the Resource Relation field. For copies of other documents, please see the Availability, Publisher, Research Organization, Resource Relation and/or Author (affiliation information) fields and/or Document Availability. |
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| Title | Medical research and evaluation facility and studies supporting the Medical Chemical Defense Program. Evaluation of the utility of human skin equivalents for studying HD-induced dermatotoxicity and evaluating antivesicant treatment regimens. Final report |
| Creator/Author | Olson, C.T. ; Snider, T.H. ; Hobson, D.W. ; Logel, C.A. ; Johnson, J.B. |
| Publication Date | 1997 Jul 01 |
| OSTI Identifier | OSTI ID: 549647 |
| Report Number(s) | AD-A--328564/0/XAB |
| Other Number(s) | CNN: Contract DAMD17-89-C-9050 |
| Resource Type | Technical Report |
| Resource Relation | PBD: Jul 1997 |
| Research Org | Battelle Memorial Inst., Columbus, OH (United States) |
| Subject | 45 MILITARY TECHNOLOGY, WEAPONRY, AND NATIONAL DEFENSE ;56 BIOLOGY AND MEDICINE, APPLIED STUDIES ; CHEMICAL WARFARE AGENTS; TOXICITY; PREVENTIVE MEDICINE |
| Description/Abstract | Two human skin culture models, also known as human skin equivalents (HSEs), and dermatomed (split thickness) natural human skin (NHS) were assessed for use in studies investigating the dermatotoxic effects of sulfur mustard BIS(2-CHLOROETHYL) SULFIDE; HD. The HSEs examined were model ZK1300 from Advanced Tissue Sciences (ATS, La Jolla, CA) and Epiderm from MatTek Corporation (Ashland, MA). NHS specimens were obtained either fresh from the Ohio State University branch of the Cooperative Human Tissue Network (Columbus, OH), or cryopreserved from the Ohio Valley Tissue and Skin Center (OVTSC, Cincinnati, OH). The utility of each skin model was assessed by histopathology and by several viability indices. Tissue samples were processed for both light and electron microscopy and examined for consistency among HSE lots and among NHS patients in exhibiting normal ultrastructural details. In particular, the samples were examined for the presence of a continuous, intact basement membrane and an epidermal-dermal interface resembling that found in normal, living human skin. |
| Country of Publication | United States |
| Language | English |
| Format | 215 p. ; PL: |
| System Entry Date | 2001 May 05 |
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Last Updated: 02/12/2009