The Health

Consequences

of SMOKING

A PUBLIC HEALTH SERVICE REVIEW : 1967

U.S. DEPARTMENT OF HEALTH, EDUCATION, AND WELFARE
           i,`. -, ' Public Health Service


Public Health Service Publication No. 1696

For sale by the 6uperluten~t of Dommsl t+3, us. oo- t RintiIlE omce
         we&bgton, D.C. 204W - Wee 60 -ta


The Federal Cigarette Labeling and Advertising Act of 1965 (Pub-
lic Law 89-92) requires that the Secretary of Health, Education, and
Welfare submit regular reports to the Congress on the health conse-
quences of smoking, together with any legislative recommendations
he may wish to make.
This 1967 Surgeon General's Report was pr&tred to provide the
Secretary and the public with a review of the research findings which
have taken place in smoking and health in the approximately 3% years
which have elapsed since the Surgeon General's Advisory Committee
issued its monumental 1964 report. Part I of this document was in-
cluded as part of the Secretary's 1967 Report which he sent to Congress
in July 196'7. Part II, which provides detailed discussions of the re-
lationship of smoking to specific diseases, is issued here for the first
time.

The 1967 report represents a review of more than 2,000 research
studies published since the 1964 report. These additional studies con-
firm and strengthen the conclusion of the Surgeon General's Advisory
Committee that: "Cigtlrette smoking is a health hazard of sutllcient
importance in the United States to warrant appropriate remedial
action."

In a separate section of this report, acknowledgments have been
made for the help of numerous scientists both within and outside the
Public Health Service, who participated in the preparation of this
report. These include the 10 distinguished scientists who made up the
Surgeon General's 1964 Advisory Committee, all of whom were kind
enough to review part I of the 1967 report before its publication. A
special word of thanks is due Leonard M. Schuman, M.D., one of the
1964 committee members, who advised the staff in the final editing of
the entire document.

SURGEON GENERAL.


Table of Contents

Part I. Current Information on the Health Consequences of
    Smolring-_-------------------------------------
      Introduction__-------------------------------
    Smoking and Overall Mortality- _ _ _ __ __ _ __ _ _ _ _ _
        Some Oeneral Considerations- _ _ _ _ _ ___ ___ __
    Smoking and Overall Morbidity-- _ __ __ _ _ _ _ _ _ _ _ _
    Smoking and Cardiovascular Diseases,,- _ _ _ _ _ _ _ _
    Smotig and Chronic B~onchopulmonary Dis-
           eases (Non-neoplastic) _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _
     Smoking and Cancer--- _ _ _ _ _ __ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _
     Other Conditiona and Areas of Research- _ _ _ _ _ _ _
      Cited References----- -----I------------------
II. Technical Reports on the Relationship of Smoking to
    Specific Disease Categories- _ _ _ __ __ _ __ _ _ _ __ _ _ _ _ _ _
    Chapter 1. Smoking and Cardiovascular Diseases-
        2. Smoking and Chronic Bronchopul-
          monary Diseases (Non-neoplastic) _
              3. Smoking and Cancer- _ _ __ __ __ _ __ _ __
         4. Other Conditions and Areas of Re-
             search--------------------------

1
3
7
11
19
25

29
33
39
41

43
45

87
125

179

V


Acknowledgments

Responsible for the preparation of this report was the National
Clearinghouse for Smoking and Health, Daniel Horn, Ph. D., director.
Staff director for this report was Albert C. Kolbye, Jr., M.D., M.P.H.,
LL.B.

The professional staff has had the assistance and advice of a number
of experts in the scientific and technical fields in and outside govern-
ment. The staff gratefully acknowledges their contributions, often
made at considerable sacrifice of time from their own professional
duties. Although space does not permit a listing of all those who have
participated in this project, the staff wishes to express appreciation
for their cooperation and help. Special thanks are due the following:
hNAHT1, FBAITC~S R., D.V.M, PH. D.-Chief, Virology and Rickettaiologs
Branch, Extramural Program, National Institute of Allergy and Infectious
Dra~aesq National Institutes of Health, Bethesda, Md.
AMES, BBUCE N., M.D.-Laboratory of Molecular Biology, National Institutes
of Health, Bethesda, Md.
hEaD, WILLTAM P., M.D.-Medical consultant, Stroke Section, Heart Disease
Control Pro&m, National Center for Chronic Disease Control, U.S.P.H.S.,
Arlington, Va.
Aunns~on, Oecq M.D.-Senior medical investigator, Veterans Administration
Hospital, East Orange, N. J.
AXEUOD, DA=, M.D.-Medical consultant, Laboratory of Biology of Viruses,
National Institutes of Health, Bethesda, Md.
Am, Srnr~nrv M., M.D.-Dire&or, Cardiopulmonary Laboratory, Saint Vin-
cent's Hospital and Medical Center of New York, New York, N.Y.
AYEW, W= R., M.D.-Medical of&X& Heart Disease Control Program, Na-
tional Center for Chronic Disease Control, U.S.P.H.S., Arlington, Va.
Bmena, JOHH J., M.D.-Head, Department of Otolaryngology, Evanston Hos-
pital, Evanston, Ill.
BEBENDES, Hews, W., M.D.-Chief, National Institute of Neurological Diseases
and Blindness, Perinatal Research Branch, National Institutes of Health,
Bethesda, Md.
Bnao, Connon W;-`Statistician, Operational Studies Section, Cancer Control Pro-
gram, National Center for Chronic Disease Control, U.S.P.H.S., Arlington, Va.
Bsa~~nna, Ronmw, M.D.-Director, oface of Director of Research, National Heart
Institute, National Institutes of Health, Bethesda, Md.
BIBG, RIO- J., MD-Professor and chairman, Department of Medicine,
Wayne State University, Detroit, Mich.
BLEBE, STIJA~~ R, MD.-Medical consultant, Heart Disease Control Program,
National Center for Chronic Disease Control, U.S.P.H.S., Arlington, Va.

VII


BOBEN, HOUS, M.D.-Clinical investigator, Veterans A-Uin Hospital,
Denver, 62010.
CABBO& BENJAMIN E.-Biometric Bknch, National Cancer Institute, National
Inetitutea of Health, Bethesda, Md,
CHADWICK, DONALD IL, M.D.-Director, National Center for Chronic Dkaae
Control, U.S.P.H.S., Arlington, Va.
DE LA PUEITTIZ, Joema L.+hief, statistical methods, Cancer Control Program,
National Center for Chronic Disease Control, lJ.S.P.&S.. Arlington, Va.
DEYKIIT, DAXIZL, M.D.-Assistant proteseor of medicine, Harvard Medical Scholl,
Boston, Masf3.
DOBBS, GEOFCO~ M.D.-Aesociate chief. Dlvlalon of Sclentitl~ Ophio~, Eweral
Trade Commission, Washington, D.C.
DOYLE, JO~EPE, M.D.-Dir&or, Cardiovascular Health Center, Albany Medical
College, Union University, Albany, N.Y.
~ETMAH, NICOLAS J., M.D.-Profmr emerlhm of obatetrl~ Johns Hopldna
Hoepital, Woman's Clinic, Baltlmore, Md.
EPSTEIN, ~lcBIC!K H., M.D.--f-r, University of Mlcbigan School of Pt&llc
Health, Department of Epidemiology, Ann Arhor, AXich.
P~ELZOE, 8. PAUL, Jr., M.D.-Deputy chief, Heart Dlaeaae Control w Na-
tional Center for Chronic Disease Control, U.S.P.ES., Arllngbn, Va
EVANS, B~BEBT, Ph. D.-Department of sodology, University of Wisconein,
Madison, Wis.
FALK, HANS L., Ph. D.-Associate scientifk dlreetor for carcinogenesb, National
Cancer InstLtute, Natlonal Institutes of Health, Bethesda, Md.
Fmurxs, BEXJAMI~J G., Jr., M.D.-Professor, Deperbnent of Physiology, Harvard
School of F'ubllc Health, Harvard University, Boston, Masc.
Fox, BE~R~BD H., Ph. D.-Acting Chief, Experimental -Branch,Injnry
Control Program, National Center for Urban and Industrial Health, U.S.P.H.S.,
Arlington, Va.
Fox, SAHUEL M., III.,. M.D.Ahlef, Heart Disease Control Program, National
Center for Chronic Disease Control, U.S.P.ES.. ArRqbn, Va.
GIITLESOHN, ALLAN, Ph. D.-Professor, Johns Hopkins gob001 of P&c Health
and Hygiene, Johns Hopkins University, Baltimore, Md
GOLD, B~XALD J.-Statistician, Operational Stndies Section, Cancer Control Pro-
gram, Na,tional Center for Chronic Disease Control, U.S.P.HB., kiington. Va.
H~SZEL, WILLIAM M.-Chief, Biometry Branch, National Cancer Institute,
National Institutes of Health, Bethesda, bfd
mm, Max, Ph. D.-Assistant chief, Biometrics Besear& Branch, National
Instktntea of Health, Bethesda, Md.
HAMMOXD, E. Cm, SC. D.-Vice president., epidemiology and statistical IX+
sea&b American Cancer Society, New York, N.Y.
HA~ELL, WILLIAM L, Ph. D.-Physical activity consultant, Heart Disease Con-
trol Program, National Center for Chronic Disease Control, U.S.P.H.S., Arllng-
ton, Va.
HAYES, RICHAR.D, D.D.S.-Assistant to the chief, cancer detection. Cancer Control
Program, National Center for Chronic Disease Control, U.S.P.H.S., Arllnq-
ton, Va.
HEIXZELXAN, F&D, Ph. D.--Research peyeholagbt, Heart Disease Control Pro-
gram, Natfonal Center for Chronic Disease tints& U.S.P.ES.. Arlington, Va.
HESS. CATHERINE B., M.D.-Assistant to the chief, Cancer Control Program, Na-
tional Center for Chronic Disease Control, U.SS,H.S., Arlington, Va.
HIGQINS, I. I. T., M.D.-Professor, University of Michigan School of Pnhllc
Health, Department of Epidemiology, Ann Arbor, Mlch.


HO~FXAX~, D~TEICJH, M.D.-Associate member, environmental carcinogenesis,
Sloan-Kettering Inatltute for Cancer Research, New York, N.Y.
IMBODEN, C. &, Jr., M.D.-Chief, (&ronic Re8piratory Disease Control Frogram,
National Center for Chronic Dleease `Control, US.P.Hd., tilington, Va.
IBXBANT, Ar.aan~ P.-Uhief, Epidemiology and SurveilMnce Branch, Injury
Control Program, National Center for Urban and Industrial Health, U.W.P.H.S.,
Arlington, Va.
KANNEL, WISXJAM B., M.D.-Associate medical director, National Heart Institute,
Harvard Medical School, Boeton, Ma&a
KENNEB, HAIEZS, M.D.-Medical c&k&ant, Heart Ditveaae Dontrol Program,
National Center for Chronic Disease Control, U.S.P.H.S., Arllngton, Va.
KOOLBYE, SUBAX~AE M+Sclentiat, Chevy Chase, Md.
Korxx, Pam, M.D.-Director, National Environmental Health Sciences Center,
Research Triangle Par& N.C.
Krkmox& Dw E.-Statl&ician, Biometrics Section. Natlonal Heart Institute,
National Institutes of Health, Beth&a, Md.
LANDAU, EMANUEL, Ph. D.-Statistical advisor, OlIlce of the Director, National
Center for Air `Pollution Control, U.S.~P.ES., Washington, D.C.
LEQAT~B, Mmvrxv S., M.D.-Chief, Cell Biology Branch, Division of Nutrition,
Food and Drug Adminletration, Washington, D.C.
LEMAISTBE, CHABLKS A., M.D.-Vice chancellor of health affaire, Otllce of the
Chancellor, University of !kxas, Austin, Tex.
LILXEHFELD, ABMHAY, M.D.-Professor, Johns Hopkins School of Hygiene and
Public Health, Baltimore, Md.

LONOIN, Hmwwr, M.D.-O&X of associate director for collaborative studies,
National Heart Institute, National Institutes of Health, Bethesda, Md.
LOUDON, R. G., M.D.-Profeeeor, Department of Internal Medicine, University
of Texas, Da&la. Ta.
MABIAND, Rx- ,E., Ph. D.-Injury Control Program, National Center for
Urban and Industrial Health, U.S.P.H.S., Arlington, Va.
MoL~Ax, Roes, M.D.-Pro-C-r of medicine (pulmonary disease), Emory Uni-
versity, School of Medicine, Atlanta, Ga.
MOBBISON, BAYA~~ H., Ph. D.-Ofllce of the Director, National Cancer Institute,
National Institutes of Health, Bethesda, Md.
MOUNT, FBANK W., M.D.-Deputy chief, Ohronic Respiratory Disease Control
Program, U.S~P.HS., Arlington Va.
MUBPHY, EDYOND A., M.D.-Associate professor, University of Colorado Medical
Center, medicine and biostatietics, Denver, Cola.
PETQLBorv, WILLXAX `F., M.D.--chief, Obstetrics and Uynecolopy Service, USAF
Ho&al Andrews, MSHCB, Andrewa Air Force Basf?, Washington, D.C.
PETTY, THOMAS L., M.D.-Assistant professor of medicine, University of Colo-
rado Medical Oenter, Denver, Colo.

Romxe, Moxrox-chief, program evaluation and project design, Heart Disease
Control `Program, <National Center for Chronic Dlaeaae Control, U.S.P.H.S.,
Arlington, Va.
Eoss, WILLIAM L., M.D.-Chief, Cancer Control Program, National Center for
`Chronic Disease Control, U.S.P.H.S., Arlington, Va.
SCHUYAN. Lxor?~an M., M.D.-Professor of epidemiology, University of Minne-
sota, School ot Public Health, Mlnneapolie, Mlnn.
%2HWAmz, J. Tnxononr., M.D.-Se&on head, Epidemiology Branch, National
In&it&e of Neurological Diseases and Blindness, National Institutes of Health,
Bethezda, Md

Ix


SHBAB, MUBBAY J., Ph. D.-Special advisor, Once of &he D&&or, National
Cancer Institute, National Imt&ut.ea of Health, Betk%b, Md
SIBEL, JAM= S., M.D.-Chief, Field Staff, CoonlinaUon and Development 8ection.
Heart DBase Control w, National Center for Chronic Dlseatx an-
U.S.P.H.S., Arlhgton, Va
STEIN- WILLXAx, M.D.-Medical o5ker. Heart Dleeaee (h&r01 Prosrmb Na-
tional Center for Ohrouk Disease Control U.S.P.H.S, ArIingbIb Vk
&BONO, JACK, M.D.-airman of Deparbaent of Pathology, Ii4mwaM state
University, Sclmol of Medicine, New Orle!am#, Ii
THO& THOMAS J.-Stati&lclan, Heart Dlmxwe Control Program, National Center
for Chronic Disease Control, U.S.P.ES.. Arlingkm, Va
UNDEBWOOD, PAUL,  M.D.- A.sUtant professor of ohste&rics and g~necolfH%Y,
University of SoutI~ Carolina MedicsI Se&& went of (Bobtrim and
Gynecology, Charleston, 8.0.
WESTUBA, EDWIR, M.D.-Chief, Applied Phyhlogy Iaboratory, Heart Diseaee
Control Program, Nbbml Center for Chronic Diaeam Control, U.S.P.H.S.,
Arlhngton, Va.
WHITE, EZIJAH L.-A&g Director, Division ai Health Interview St&bt&
National Ceuter for Health Statistics, U.S.P.H.S., Wahhgb, D.C.
WOOLSEY, TH~DOM D.-`Deputy director, O&e d the Center Dhector, National
Center for Health Statietlca, U.S.P.&III, Washington, D.C.
WYRDEB, E~NBST L., M.D.-Associate member, Sloan-Kettering Institute for !kh-
nology, New York, N.Y.
YAKMOLINSKY, ~CEAEL, M.D.-tid chemist, Ircaborntiry d ~Ohcuhr
Biology, National Infkitutea of Health, Beth- Md
ZUKEL, WILLIAM J., MD.-Aseociate Dkeetor for Cdlabomtlve Studies, National
Heart Institute, National Inatltutes of Health, Bethesda, Md
The following professional staff of the National Clearinghouse for
Smoking and Health contributed to the preparation of this report:
Robert F. Clarke, Ph. D., Emil Con-in, Bobert S. H&hings, Selwyn
Waingrow, and David G. Wember, M.D.

X


PART 1

Current Information on the
Health Consequences of
         Smoking


Introduction

I n January 1964, an Advisory Committee appointed by the Surgeon
General of the Public Health Service issued its report (16) on the
relationship between smoking and health.* The conclusions of that
&nmittee were summed up in the sentence: uCigarette smoking is a
health hazard of sutllcient importance in the United States to warrant
appropriate remedial action."
In the 3% years since the publication of that report, an unprece-
dented amount of pertinent research has been completed, continued,
or initiated in this muntry and abroad under the sponsorship of
governments, universities, industry groups, and other entities. This
research has been reviewed and no evidence has been revealed which
brings into question the conclusions of the 1964 report. On the con-
trary, the research studies published since 1964 have strengthened
those conclusions and have extended in some important respects our
knowledge of the health consequences of smoking.
The present state of knowledge of these health consequencB8 can,
in the judgment wf the Public Health Service, be summarized as
follows :

1. Cigar&a smokers have substantially higher rates of death
and disability than their nonsmoking counterparts in the popu-
lation. This means that cigarette smokers tend to die &, earlier
ages and experience more days of disability than comparable
nonsmokers.

2. A substantial portion of earlier deaths and excess disability
would not have occurred if those affected had never smoked.
3. If it were not for cigarette smoking, practically none of the
earlier deaths from lung cancer would have occurred; nor a sub-
stantial portion of the earlier deaths from chronic bronchopul-
monary diseases (commonly diagnosed as chronic bronchitis or
pulmonary emphysenra or both) ; nor a portion of the earlier
deaths of cardiovascular origin. Excess disability from chronic
pulmonary and cardiovascular disectsss would also be less.

o ?????o? and Health. Report of the Advisory Cmnmittee to the Surgeon
~~~1 of the Public Health Service." It la frequently r&err& to in t& mann-
SeriPt a8 "the SW General'8 1964 Report."

3


4. Cessation or appreciable reduction of cigars& smoking
could delay or avert a substantial portion of deaths which occur
from lung cancer, a substantial portion of the earlier deaths and
excess disability from chronic bronchopulmonary diseases, and a
portion of the earlier deaths and excess disability of cardiom-
cular origin.

NATURE OF RECENT RESEARCH FINDINGS

Since the Surgeon General's Report was published in January 1964,
there has been a proliferation of additional studies and reports on
smoking research. In the 12 years preceding that report, some 3,000
articles were published reporting research; since 1964, there have been
more than 2,000 additional studies.

These studies have helped to clarify the role that age plays in the
relationship of smoking to health ; the similarities and diiIerences in
the ways in which men and women are affected by smoking; and the
influences and effects of stopping smoking, particularly in the case of
lung cancer where there is significant data to show thst sharp redue
tions in lung cancer deaths follow closely reductions. in cigarette
smoking. The studies also suggest the importance of a variety of
measures of exposure ; add substantial new information on the magni-
tude of the morbidity problem associated with smoking; and provide
more adequate data upon which to base estimates of the magnitude
of the mortality problem.
Historically, concern about the effects of smoking began with ob-
servations of the extremely high frequency with which lung cancer
patients were identified as cigarette smokers. These observations took
on a fuller meaning with the first publication of the prospective studies
in 1954 when higher overall death rates among cigarette smokers
were identified. The rates were found to exceed the difference that
could be accounted for by lung cancer alone. Until that time, the
possibility remained that although more cigarette smokers appeared to
suffer from lung cancer, if there were no significant excess overall
mortality, some other cause or causes of mortality would have had to
be underrepresented among cigarette smokers.

The Surgeon General's 1964 Report concluded that cigarette smokers
do have higher death rates than their nonsmoking counterparts. Thii
has changed the emphasis of the present problem away from the ques-
tion "does cigarette smoking cause diiP' to the more precise
questions of :
    1. How much mortality and excess disability are associated with
smoking?

4


    2. How much of this early mortality and excess disability would
not have occurred if people had not taken up cigareti smoking?
  3. How much of this early mortality and excess disability
could be averted by the cessation or reduction of cigarette
SlllOkillg?
  4. What are the biomechanisms whereby these effects take place
and what are the critical factors in these mechanisms?
To answer these questions one must not only study the details of
the relationship of ovdl mortality with cigarette smoking, one must
also turn to the specific causes of death and disability and to other
kinds of evidence.
The research carried on since 1964 is of three principal varieties :
Epidemiological studies, especially those which involve surveys of
large portions of the population ; a health survey which has revealed
new information about the relation between smoking and illness; and
a vast amount of experimental, clinical, pathological, and behavioral
research which adds to the understanding of the precise ways in which
smoking affects the body, plus other closely related or peripheral
information.
In the area of morbidity or `illness, the primary addition to our
knowledge is from "Cigarette Smoking and Health Characteristics,"
a report (16) of the National Center for Health Statistics on the
frequency of illness among smokers and nonsmokers in a large proba-
bility sample of the U.S. population. Regarding epidemiologioal data,
new reports from four of the major population studies have been
published since 1964 :
  1. The Dorn study of smoking and mortality among U.S. vet-
  erans (18).
  2. Hammond's study on smoking in relation to the death rates
  of 1 million men and women in 25 States (11).
  3. The Doll and Hill study on the mortality of British physi-
  cians in relation to smoking (8,&M).
  4. A Canadian Smoking and Health Study of Canadian pen-
sioners, including veterans and dependents (1).
The principal features of the additional data provided by these
four studies are.: (1) The extension of the time period of followup,
(2) the additional data available for specific age groups among men,
and (3) the inclusion of substantial data on women. In all, the pro-
spective study reports now available are based on more than 108,000
dwths, an increase of about 43,000 deaths over the 65,023 summarixed
in the 1964 report. About 19,960 of these additional deaths were among
women.


THE NATURE OF THIS REPORT

This report which provides a summary of current information on the
health consequences of smoking, is based on the review of the research
reports which have become available since the study of the Surgeon
General's Advisory Committee was releasexl. Public Health Service
staff members consulted the literature and requested additional infor-
mation or interpretations of the published data from the research
scientists when needed. During this review a complete bibliography,
containing some 5,766 citations, was compiled; it is .now in manu-
script form and will be published shortly (19).
The advice and comments of experts within the Public Health Serv-
ice, particularly the Bureau of Disease Prevention and Environmental
Control and the National Institutes of Health, as well as of specialists
outside the Public Health Service, were solicited especially on matters
involving judgment and evaluation.
The general criteria used by the Surgeon General's Committee have
been followed. First, epidemiological data were evaluated to determine
whether an association exists. In judging the significance of the as-
sociation, its consistency, strength, specificity, temporal relationship,
and coherence were utilized. The convergence of evidence from animal
experiments, clinical and autopsy studies, and population studies re-
mains the essential basis for evaluation of the significance of the
associations identified.

This report presents, under the following headings, the major find-
ings of research studies published in the past 3 to 4 years :
1. Smoking and Overall Mortality.
2. Smoking and Overall Morbidity.
3. Smoking and Cardiovascular Diseases.
4. Smoking and Chronic Bronchopulmonary Diseases (Non-neo-
    plastic).
5. Smoking and Cancer.
  6. Other Conditions and Research Areas.
Each of these sections is introduced by pertinent conclusions from
the Surgeon General's 1964 Report, which are followed by discussion
and conclusions of the present study.


Smoking and Overall Mortality

   CONGLIJNONS OF TEE SURGEON G ENElrAL's 1934 RsPoRT

CIGARETTE smoking is associated with a 70-percent increase in
the age-spe.cXc death rates of males, and to a lesser extent with in-
creased death rates of females. The total number of excess deaths
causally related to cigarette smo -II0 in the U.S. po ulation cannot
be accurately estimated. In view of t  continuing 811 if mounting evi-
dence from many sources, it is the judgment of the Committ.sc that
cigarette smoking contributes substantially to mortality from certain
specific diseases and to the overall death rate.

hi In general, the greater the number of cigarettes smoked daily, the
% her the death rate. For men who smoke fewer than 10 cigarettes
a ay, according to the seven
all causes is about 40 percent tl respective studies, the death rata from

who smoke from 10 to 19 ci   `gher than for nonsmokers. For those
        %T rettes a day, it is about 70
than for nonsmokers ; for t ose who smoke 20 to 39 a cir rcent higher

hi                          y, 90   cent
her; and for those who smoke 40 or more, it is 120 percent
&!                  KY?
igarette                       `gher.
     smokers who stopped smoking before enrolling in the.
seven studies have a death rate about 40 percent higher than non-
smokers, as against `70 percent higher for current cigarette smokers.
Menwhobegansmo'
     9
death rata than those w before age 20 have a substantially higher
          o began after age 25. Compared with non-
smokers, the mortality risk of cigarette smokers, after adjustments for
differences in age, increases with duration of smoking (number of
years), and is higher in those who stopped after age 55 than for those
who stopped at an earlier age.

In two studies which recorded the degree of inhalation, the mortality
ratio for a given amount of smoking w&s greater for inhalers than for
noninhalers.
The ratio of death rates of smokers to that of nonsmokers is hi  est
at the earlier ages (44-50)          %I
with increasing age.    represented in these studies, and dec *  es

Possible relationships of death rates to other forms of tobacco use

were also investi  ted
than 5 cigars a r  * * *. The death rates for men smoking less
       ay are about the same as for nonsmokers For men

Smoking more than 5 ci T rs daily death rates are slightly higher.
There is some indication t at these higher death rates occur  rimaril
in men who have bean smoking more than 30 years and w o i inhd
the smoke to some d
if at all hi her than or nonsmokers, even for meu who smoke 10 or
  ifll   Y . The death rates for pipe smokers are little

more pipe
30 years.  s a day and for men who have smoked pipes more than

271-224o-67-2                           7


CURRENT INFORMATION, 1967

The primary addition to knowledge in the nreas of smoking and
overall mortality comes from the four major population studies. Ad-
ditional periods of followup have provided a broader base from which
it .becomes possible to estimate the excB8s deaths related ,to cigarette
smoking in the U.S. population and from which firmer conclusions
may be drawn as to the role of various exposure factors in the associa-
tions found.

The contributions since 1964 of each of the four population studies
to the relation of smoking and overall mortality, as summarized by the
authors, are set forth below.

&tTJlY OF U.S. VETERANS

  (An 854 year followup of 293,658 persons holding U.S. Government
  life insurance polides. Commonly referred to as the Dom Study after
  the late Dr. Harold F. Dom. The most recent report is by Kahn (IS).)
"* * * the increased mortality risk associated with cigarette smok-
ing was found to be higher in the more recent calendar time period
than in khe initial years of the study.
"* * * mortality ratios of current cigarette smokers compared with
those who have never smoked are 1.7 for death from all causes, 10.9
for lung cancer, 12.2 for ssnphysema without bronchitis, and 1.6 for
coronary heart disease. Paralysis agitans was the only cause of death
associated with significantly lower mortality for smokers than for
nonsmokers.

"For all categories of current smokers, risk was related to amount
smoked. The risk for cigarette smokers was much greater than that for
pipe or cigar smokers. Current smokers of cigarettes, cigars, or pipes
experienced a mortality risk significantly greater than that for non-
smokers if they smoked more than four pipes or four cigars daily or
more than an occasional cigarette.
"There was a positive relationship between duration of cigarette
smoking and mortality risk from all causes of death for at least some
classifications of smokers,

"* * * probabilities of death for ex-smokers of cigarettes revealed a
downward trend in risk as duration of time discontinued increased,
when other variables--age began smoking, amount smoked, and cur-
rent age-were controlled * * *. The data can be regarded as evidence
against the constitutional hypothesis."
Calculations are presented to note that observations made during
the study suggest the possibility that data from respondents (those
who answered the smoking questionnaire) may in fact underestimate

8


the risk associated with smoking. The Surgeon General's 1964 Report,
had considered the possibility that di&ences between respondents
aud nonrespondents to the questionnaire might have introduced a
bias and had attempted to calculate a maximum estimate of that
bias.

STUDP OF MEN AND WOIKICN IN 25 STATIW

(This report is baaed on 3,764,671 Wrson-years of experience and 43,!2!21
deatha occurring among l,OQ3,ZZQ eubject8-M ,668 men and S.3,671
women-between the ages of 35 and 34 from October 1.1969. to Feb-
ruary 16,lQ60, when they enrolled in a proepe&ve study and answered
detailed queetionnairea including questions on their smoking habits.
Hammond. (II) .)

"Death rates of both men and women were higher. among subjects
with a history of cigarette smoking than among those who never
smoked regularly.

"Death rates of current cigarette smokers increased with number of
cigarettes smoked per day and degree of inhalation.

"Death rates were higher among current cigarette smokers starting
the habit at a young age than among those starting the habit later in
life. Among both men and women, the difference between the death
rates of cigarette smokers and nonsmokers increased with age.

"Among men, ,the death rates for ex-cigarette smokers were lower
than for men currently smoking cigarettes when they enrolled in the
study. Death rates of ex-cigarette smokers decreased with the length
of time since they last smoked cigarettes.

"* * * Total death rates and death rates from most of the common
diseases occurring in both sexes were higher in men than women,
were higher in men who never smoked regularly than in women who
never smoked regularly, and were far higher in men with a history
of cigare6t.e smoking than in women with a history of regular cigarette
smoking.

"The difference ,between the death rates of subjects with a history
of cigarette smoking and subjects who never smoked regularly was
far greater among men than women. Female cigarette smokers (as
a group) have been far less exposed to cigarette smoke than male
cigarette smokers of the same ages, as judged by number of cigarettes
smoked per day, degree of inhalation, and the number of years they
have smoked. Many female cigarette smokers smoke only a few
cigarettes a day, do not inhale, and have been smoking for only a
few years; their death rates are about the same as the death rat.4~ of
women who never smoked regularly."

STUDY OF BRITISH P~~~CIANS

(The mortality of nearly 41,000 men and women in the medical profes-
sion in the TJnH.4 Kinflom has been followed for I.2 yeara During the

9


tit 10 yeara 4,697 of the men and s&B ai the women died. These deaths
were analyzed in relation to smoking habits reported by doctors in reply
  to a questionnaire sent to them in 196l-both sexes-au d again in lOti?,
  men, and 1090, women Doll and Hill (8, 9).)
`;* * * An association with smoking is found, in differing degrees,
in men for seven causes of death ,[which accounted for 39 percent of
the death rate]-namely, cancer of the lung, cancers of the upper
respiratory and digestive tracts, chronic bronchitis, pulmonary tuber-
culosis, coronary disease without hypertension, peptic ulcer, and
cirrhosis of the liver and alcoholism. No association is found with the
remaining 61 percent of the death rate, and this includes such major
causes as other forms of cancer, cerebrovascular accidents, hyperten-
sion, myocardial degeneration, suicide, and accidents.
"In women, the few deaths at present available show an association
only between smoking and cancer of the lung.
U* * * If the excess deaths in smokers under the age of 65 years
from (a) cancer of the lung, (6) chronic bronchitis and emphysema,
(c) coronary thrombosis without hypertension be taken as attributable
to their cigarette smoking, then the total mortality from all causes at
ages 45-64 years is increased thereby by approximately 50 percent."
The report states: "One of t.he striking characteristics of British
mortality in the last half-century has been the lack of improvement
in the death rate of. men in middle life. In cigarette smoking may lie
one prominent cause."

STUPY OF CANADUN PENEIONPXS

(The purpose d the study was to investigate the relationships between
residence, mtion, smoking ~habits, and mortality from chronic
Waaes .@cularly lung cancer. It was initiated by a que&ionndre
which was sent to Canadian veteran pension recipients doriug the
period September 1955 through June 196g.

Returns from ?S#OO men, and 14,000 women, mostly widows, were
analped. The men were mainly World War I and World War II vet-
erans, but some Boer War and Korean War veterans, as well aa some
non-veteran pension recipients were included. The age of most of the
men at the beginning of the study ranged from 30 to 90 years and the
distribution was characterised by the ages of men eligible for service
in the two World Wars.

For each respondent dying between July 1. 1956, and June 30, 196%
the cause oi death was related to information on his questionnaire
atint age, history of emoking habits, residence and occopation.
Among the respondents during the 6 years of followup there were Q&l
deaths of males, and l,?fM deaths of females which were analyaed
  (11.1
"Current cigarette smokers had a death rate for overall mortality
54 percent higher than that of nonsmokers * * * Ex-cigarette smok-

10


ers had a comparatively lower rate, which was still 36 percent above
the rate for nonsmokers * * * Men smoking combinations of ciga-
rettes plus cigars and/or pipe also had elevated death rates for overall
mortality, but these were not elevated to the same extent as those of
men smoking only cigarettes.
"The death rates for overall mortality of pipe smokers and cigar
smokers were not appreciably different from those of nonsmokers.
"For cigarette smokers as compared to nonsmokers, overall mor-
tality ratios were elevatd after 5 years of smoking at any time in
their life and remained elevated as long as they amtinued to smoke
cigarettes.

"Male current cigarette smokers who inhaled had a death rate for
overall mortality 52 percent higher than that of those who did not
inhale.

"An urban/rural comparison was made between males of equivalent
cigarette smoking habits and nonsmokers. It was found that the
death rate for overall mortality of urban dwellem @rsons with
a history of 5 years or more of city residence) was 12 percent higher
than that for rural dwellers of comparable smoking habits.
"Respondents were classified into occupational groups based on
their history of occupation. No evidence was found in this study of
clear-cut associations between cause of death and occupation. Further,
occupation did not appear to modiQ the e&ab%shed association of
cigarette smokers with death rates in ~XCCZB of those of nonsmokers."

SOME GENERAL CONSIDERATIONS

The problem of how best to measure the relationship between smok-
ing and mortality has been discussed in the Surgeon General's 1964
Report as well as in some of the proqective study reports. &4 the
amount of data available increases, the person-v of observations
in the many population subgroups that are worth examining increases
so that stable rates may be computed and compared. A brief discussion
of three measures of comparison available and their utility seems
desirable as confusion frequently arises over these measmes.
  1. Mortality Ratios: Obtained by dividing the death rate for
a classification of smokers by the death rate of a comparable
  group of nonsmokers.

2. Differences in Mortality Rates: Obtained by subtracting
from the death rate for smokers, the death rate of a comparable
group of nonsmokers.

3. Excess Deaths: Obtained by s&ra&ng from the number
of deaths occurring in a group of smokers, the number of deaths

11


which would have occurred if that group of smbkers had ex-
perienced the same mortality rates as a comparable group of
nonsmokers. In the example which follows this has been reported
as a percentage of all deaths in the appropriate age group.
Table 1 presents in summary form all three measures for five age
groups of men from both the U.S. veterans study and Hammond's
study and for the same age groups of women from the latter study.
The statistics were derived from the cited publications to provide
reasonable comparability and may vary slightly from the Bgures
combined in other ways. Also it should be noted that the age groups
are not defined identically and the experience reported covers some-
what different time periods. The smoking group analyzed is "cur-
rent cigarette smokers," i.e., those who were smoking at the time of
enrollment into the study, and the comparison group is %ever smoked
regularly," i.e., those who had never been regular smokers of any
form of tobacco.

The nmnber of deaths in each age-sex group is given to indicate
the relative stability of the figures in that column. The data in the
veterans study are largely concentrated in age groups X5-64 and
65-74. In Hammond's study, age group 3544 is less stable than the
succesding groups both for men and for women.
1. Morta&y Rat&x-For men, these are at their highest in
age group 45-54, diminishing in each subsequent decade. In both
studies mortality ratios appear to be somewhat lower in the preceding
decade 35-44. However, with the smaller numbers of cases available
in that age group, it may be that selective factors contribute to the
fluding. For women the mortality ratios are much smaller than for
men, although the same pattern is suggested. In general, a mortality
ratio has been considered to reflect the degree to which a classiflca-
tion variable identifies or may account for variations in death rates.
As such, it is a measure of relative risk which indicates the importance
of that variable relative to uncontrolled variables-an indicator of
phi?&zl biologicaz 8ifpG-8.

2. Difftmncerr ila Mort&y Ratea.-These increase consistently
with increasing age in all three study groups, except for the oldest
age group in women where there is practically no difference in the
rates for smokers and nonsmokers. Differences between smokers' rates
and nonsmokers' rates are much smaller for women than for men,
as are the death rates themselves for men and women classified simi-
larly with respect to smoking. This measure reflects the added proba-
bility of death in a l-year period for the smoker over that for the non-
smoker. As such it is a measure of peraon.& health &g&@n.ct?, a means
for the individual to estimate the added risk to which he is exposed.

12


TABLE l.-Compariam of 3 nmmm+s of relatkhip betwem cigarette
smoking and ovem?~ death rates by age and 8ex a8 derived from W
major proepective 8tu4698 (11,13)'

U.S. VETNRANB: &N

   Total deaths __________ _____,
Death rates per 100,000:
  Never smoked regularly _______,
  Current cigarette smokers- _ _ _ _.
Mortality ratio * __________________.
Difference in death rates per
100,000 * ----_-_--------__-----.
Exceaa deaths as percentage of
totsl'-------------------__---_.

   Total deaths __________ ___ __.
Death rates per 100,000:
  Never smoked regularly _______.
Current cigarette smokers- _ _ _ _
Mortality ratio f _______ __ ___ _ ___ __.
DiSerence in death rates per
100,000'~~~____~~~~~________~~.
Excess deaths as percentage of
total'----______-_-_______-----.

HAMMOND WOMEN

   Total deaths ___________ _ ___.
Death rates per 100,006:
Never smoked regularly-- __ _ ___
Current cigarette smokers-----.
Mortality ratio * ____________ ______.
DSerence in death rates per
100,000~ _-_____________________
Excess deaths aa percentage of
total'-----_--_---------_-----.

383    366  13,840  17,550  1,932


127    264   1, fm  2# 411  6,214
232    723   1,819  4032  s, 471
1. 83    2 76    L 72    L 67   1.36


105    464    763   1,621  5257

  33    43     21     17     8

631  5,297


210    406
397    925
1. 89   228


187    519


33    33

4125  39968

9168  7, a63
4788  9,674
1. 51   123

1, 620  1,811

  13     4

727


105
186
1. 13


21


  5

Kg=

304
334
L26


  30


  9

3,915

698
333
1. 20

5,115

1,913
s=
L 17

146


4

316

2

r,l@

5,914
5,846
.99


  68


-----

48-k

65-74

76-84
---


3. Eacea Deaths aa a Percentqe of Total Death-h with mor-
tality ratios, this statistic appears to be highest in the age group 45-54
where it reaches 43 percent in one group of men and 38 percent in
the other. Hammond's data by B-year age groups show the highest
rate at ages 45-49, where it is 44 percent. Reviewing both study groups
it appears that for men between the ages of 35 aud 60 approximately
one-third of all deaths that occur are excess deaths in the sense that
they would not have occurred as early as they did if cigarette smokers
had the same death rates as the nonsmoking group. For women, the
percentage is much lower, reaching a peak of 9 percent of all deaths
in age group 45-54. It should be noted that this measure not only de;
pends on the differences in death rates between the smokers and the
nonsmokers, but also on the proportion of smokers in the group. Thus,
even with a large difference in rates between smokers and nonsmokers,
a population with very few smokers would have very few excess
deaths. This measure is therefore an indicator of the p&Z& hea.Zth
Bisnificymrce of the differences found since it measures the number of
people affected and therefore the magnitude of the problem for society
as a whole.

Once the magnitude of the excessis identified the problem becomes
one of determining (1) how much of the excess would not have oc-
curred if it had not been for cigarette smoking and (2) how much
would have occurred anyhow. It should be noted that much of the ex-
cess has already been identified as belonging in the first category. Of
the remainder, little of the excess has been clearly identified as belong-
ing in the second category-t.hat is, not caused by smoking. With most
of that remainder there is uncertainty as to the category in which it
belongs.

Studies involving smoking, whether epidemiological or ,behavioral,
have been concerned with measures of exposure to tobacco smoke. For
the most part, these studies have been restricted principally to the in-
dex of number of cigarettes smoked over a spe&ed period of time,
usually an "average day." The heavy reliance on numbers of cigarettes
alone as a measure has produced important findings but it has possi-
bly obscured others. The new reports on the prospective studies have
provided a substantial amount of data to support the concept that
many elements should entsr into an overall measure of exposure. Such
factors as age at beginning smoking, duration of smoking, and inhala-
tion have all shown some independent contributions to the overall
effect, along with numbers of cigarettes. A recent report (16) has at-
tempted to develop a more adequate measure of exposure in which
various individual components of dosage would be combined to form
compositescores.

14


A dosage score was developed as a function of the average number
of cigarettes smoked per day, the "tar" (smoke solids minus moisture)
rating of the brand of cigarette smoked, and the portion of the ciga-
rette actually smoked. In addition, questions on both depth aud fre-
quency of inhalation were developed. Normative data have been ob-
tained from a national survey sample of smokers. In general, although
the various measures reflecting exposure are interrelated, there are
many individuals with high exposure on one measure but low ex-
posure on another. Furthermore, there are sysbma& differences in
some of these measures of dosage between men and women, between
heavy and light smokers (by the usual criterion of numbers of ciga-
rettes) , etc. The existence of a dose-response relationship between ex-
posure to cigarette smoke and the risks most clearly associated with
cigarette smoking is now generally accepted.
Wynder .and 4Hoffmann (90) have shown in laboratory experiments
with animals that the tumorigenicity of cigarette smoke can be
reduced by alteration in the cigarette which reduces the "tar" and nico-
tine content. They use the term "indicator" for %r" and nicotine con-
tent (the two measures tend to be used jointly since when one is high
the other tends to be high unless the nicotine has been removed in
processing), or other measures which reflect this Qpe of relationship,
lacking the identification of specific agents which are responsible for
the effect. Bock, Moore, and Clark (8) have independently shown a
similar variation in carcinogenic activity of tobacco Yati' obtained
from different types of cigarettes.
The preponderance of scientific evidence strongly suggests that the
"tar" and nicotine content of cigarette smoke is a meaningful factor in
the measurement of dosage.

The cessation of smoking is, of course, an extreme example of the
reduction of dosage. Data from the prospective studies show a reduc-
tion in both overall mortality axid mortality from specific diseases
among those who have stopped smoking when compared with those
persons who continue to smoke. This finding has been somewhat ob-
scured by the fact that ill health is a frequent cause of giving up
smoking so that death rates and disability rates for ex-smokers as a
group tend to be high for an initial period of time following cessation.

In this connection, the Study of British Physicians shows that
among the total group of physicians in the &udy (smokers, ex-
smokers, and those who never smoked, combined) there was a reduc-
tion in the standardized lung cancer death rate from 0.69 per 1,000
in the first 5 years of the study (1951-56) to 0.64 per 1,000 in the sec-
ond 5 years of the study (1956-61). This reduction occurred during

16


the time when there was also a substantial drop in cigarette smoking
among physicians in general, and during the time that lung cancer
rates were risii in the male population of Great Britain. This situa-
tion is not unlike that of a controlled cessation experiment in which
the effect of giving up smoking is judged by the mortality results in
an entire population in which the giving up of smoking is common
as against another population in which it is not common. A more recent
report by Doll (7) suggests that this trend is becoming more marked
as the rate of smoking among British physicians decreases and the
length of the cessation period increases.
These Endings are shown in Table 2, which has been derived from
Doll's report (7). The lung cancer death rate among men in England
and Wales increased from 1.49 per 1,000 in the period 1954-57 to 1.86
per 1,000 in the period 1962-64, a rise of 25 percent. At the same time,
the lung cancer death rate for British physicians dropped from 1.09
per 1,000 in &he first period to 0.76 per 1,000 in the second period, a
reduction of 30 percent. This reduction in death rates from lung can-
cer among all physicians is larger than would have been anticipated
from examining only the experience of those physiciaus who had
stopped smoking before the study began and indicates that the ex-
perience of ex-smokers in prospective studies probably understates
the benefits of giving up smoking.
With these fmdings the case for cigarette smoking as the principal
cause of lung cancer is overwhelming. The reduction of rates experi-
enced in ex-smokers as compared with continuing smokers is clearly
shown in the case of lung cancer to be a reflection of a sign&ant
change in risk. Since the concern that selective bias might have ac-
counted for the earlier findings has been contraindicated, a stronger
case csn now be made for interpreting reduced rates of overall mor-
tality for those who give up smoking as also reflecting a direct ahera-
tion of risk compared to those who continue to smoke.
There are no adequate data to evaluate the benefit of reductions in
exposure that are more mode& than those achieved by complete ceesa-
tion, although it seems reasonable to assume that a substantial reduc-
tion in exposure is likely to be accompanied by some reduction in risk
relative to those who do not reduce their exposure.

16


TABLE 2 .--changes
sicians     in the lung canw death rate in male British phy-
   (age 36-84) compared wit% chunges in the &es f&r talc ma&
popu.?ation of England and Wales for 3 time G&sv& bebxcn 1964
and 1964 (7)

The period

1954to 1957___________________________________
1958tJo 1961------______-______________________
1962to 1964--------____--_____________________
Percentage change:

1st to 2nd period- __ _ ___ _ __ _ ___________ _____
2nd to 3rd period---- _____ -- ________________
lstto 3rd period---_________________________

+15
++a%

-24
-8
-30

17


Smoking and Overall Morbidity

T TEIE TIME of the Surgeon General's 1964 Report there was no
A information available on the overall disability associated with
smoking. To investigate the relationship between smoking and mor-
bidity, the National Center for Health Statistics of the Public Health
Service introduced questions about cigarette smoking into its National
Health Survey, beginning in July 1964. This Survey is a continuing
study conducted since 1957.

In carrying on this Survey, interviewers each year visit 42,000 fami-
lies (selected as a probability sample of the civilian, noninstitutional
population of the United States) and question them about illness, dis-
ability, and days absent from work ,because of illness, as well as the
nature of the illness. In the year ending in June 1965, they inquired
(after all other questions about health had been asked) about the smok-
ing habits of persons in the family who were 17 years of age or over.

The National Health Survey is concerned with three overall meas-
ures of the impact of illness.
1. Daya Loat Frcnn Work.- These are days absent from job or busi-
ness because of illness or injury. They apply only to those persons
who are currently employed and are therefore heavily concentrated in
age groups 17-64.
2. Bed Day&-These are days when the person is su5iciently ill or
disabled so as to spend all or most of the day in bed, either at home
or in a hospital. All days spent as a hospital patient are included.
3. Days of Restricted Activity.-These are days when a person cuts
down his usual activities for most of a day because of an illness or
an injury. Days lost from work because of illness and bed days are, of
course, counted as days of restricted activity. This represents the most
general measure of, disability available in the United States today.
Table 3 summarizes the findings in a form similar to that used for
summarizing the overall mortality utilizing three measures of mor-
bidity effect : Morbidity ratios, differences in rates, and excess days of
disability.

19


TABLE 3.-tZlnnpa&m of 3 mexuurea of relakhip between cigar&
smoking and 3 types of didiZity days bg age and 8cx as cikiuedfrom
the Nat&maZ Et&h &uvcg (16)

WOESLoss DAYS

E&hated total days (millions)----..
Rate: '

112   127    21    80    5s     4

  Never smoked cigarettea _______   3.4   5.6   9.8   4.5   5.3
  History of cigarette smoking----   4.4   8.5   9.8   6.5   6.9
Morbidity ratio *-- _______________   1.3   1.5   1.0   1.4   1.3
DBerence in morbidity rhea * I--- _ _   1.0   2.9    0   2.0   1.6
Excess days as percentage of total a-   20    28     0    18    11

RESTEICTED ACTIVITY Days

Estimated t&al days (millions)-----
Rate: 1

305   386   271   543   489   395

  Never smoked cigarettea _______  7.5  15.0  32.9  13.3  22.6   40.1
  History of cigarette smoking- _ _  10.6  22.9  37.9  17.8  25.3   44.8
Morbidity ratio *--- ______ _ _ _ _____   1.4   1.5   1.2   1.3   1.1    1. 1
DiiTerence in morbidity ratea I4--- _ _   3. 1   7.9   5.0   4.5   2.7    4.7
Excess days as percentage of total I-   23    28    8    14    5     2

BED DAYS

E&ii&xl total daya (millions)-----
Rate: '

111   118

Never smoked cigarettes _______  2.7   4.6
  History of cigarette smoking- _ _  3.9   6.9
Morbidity ratio I-- _ --_-----______   1.4   1.5
Difference in morbidity rates * 4--- _ _   1.2   2.3
Excess days as percentage of total I-   23    28

AhIn

T

17-44

46-M

Bsnlld
OVW

100


13.4
13.0
.97
-0.4
-1

5.0
:
:

210   168    146

5.4   8.0   15.1
6.7   9.2   15.2
1.2   1.1    1.0
1.3   1.2    0.1
10    6     0

B5aud
OVW

' DiU- ln hfmbldity Ii&a-itforbidifv rate for c&art& amokem minas morblditv rota for thuw who
twmbmob4d~df.w.

` Exceaa deatha among c@rette amokas (I.E., addltionnl days o[ dlmbfflty that omur among c@mtte
smokem prr yew dove thorn whkb would hme ocamed li amokexa had the name rated aa thm who nevs
smoked cigamttss). TUJ Is expmmmd a~ a percmm of oil diw&Uity dnys ooxmfng in that ale-m gronp.

DAYS LOST FROM WORK

For those with a history of cigarette smoking, classified by heaviest
amount smoked, the average number of days was 7 percent higher for
men and 15 percent higher for women who had smoked less than 11
cigarettes per day; 33 percent higher for men and 60 percent higher

20


for women who had smoked 11-20 cigarettes per day ; 48 percent higher
for men and 79 percent higher for. women who had smoked 21-40
cigarettes per day; and 33 percent higher for men and 140 percent
higher for women who had smoked more than 40 cigarettes per day.
The relationships expressed by all three measures are somewhat higher
among men aged 45-64 than among men aged 17-44, but lower among
women aged 45-64 than among women aged 174. In the survey year,
there were an estimated 399 million workdays lost in the United States
because of illness. A total of `77 million days, or 19 percent, were excess
workdays lost because of the higher rates which exist among persons
who have ever smoked cigarettes as compared to those who never
smoked. This excess loss is highest in men 45-64 where it represents
28 percent of all days lost.

BED DAYS

For those with a history of cigarette smokiug, class&d by heaviest
amount smoked, the average number of days was 10 percent higher
for men and 4 percent lower for women who had smoked less than 11
cigarettes per day ; 22 percent higher for men and 17 percent higher
for women who had smoked 11-20 cigarettes per day; 22 percent high-
er for men and 57 percent higher for women who had smoked 21-40
cigarettes per day; and 53 percent higher for men and 192 percent
higher for women who had smoked more than 40 cigarettes per day.
Relationships with smoking are higher for men than for women for
all three measures except for age 17-44 in which the d.ifTereuces in mor-
bidity rates between smokers and nonsmokers are about the same. For
the entire population 17 years of age and older there were an estimated
853 million bed-days in the survey year. A total of 88 million of these
days, or 10 percent, were "excess" days lost because of the higher rates
which exist among persons who have ever smoked cigarettes as com-
pared to those who never smoked. Excess days as a percentage of total
bed-days is highest for men aged 45-64, where it is 23 percent.

DAYS OF RESFRICTED ACTIVITY

For those with a history of cigarette smoking class&d by heaviest
amount smoked, the average number of days was 12 percent ,higher
for men and 4 percent higher for women who had smoked leas than
11 cigarettes per day ; 32 percent higher for men and 22 percent for
women who had smoked 11-20 cigarettes per day; 39 percent higher
for men and 43 percent higher for women who had smoked 21-40
cigarettes per day ; and 81 percent higher for men and 146 percent
higher for women who had smoked more than 40 cigan&.es per day.
Again rates are higher for men than for women in all three measures
except for age group 1744, in which differences in morbidity rates
are higher for women. There were an estimated 2,369 million such days


in the survey year; 306 million, or 13 percent, were excess days lost
because of the higher rates which exist among persons who have ever
smoked cigarettes as compared to those who never smoked. Excess
days as a percentage total restricted activity days was highest in men
aged45-64.
To help evaluate these general indices of morbidity as measured by
various kinds of disability days it is necessary to turn to the conditions
which are reported more frequently by cigarette smokers than by non-
smokers. Since these are either self-reports or reports made by a re-
sponsible member of the household for others in the household, the
diagnostic accuracy of the reports is obviously less than one could ob-
tain from direct medical examination. Nevertheless, the bulk of the
reports on chronic conditions reflect what a physician has previously
told the patient or the family with regard to a diagnosis of the
condition.
Chronic conditions (one or more) are reported by 11 percent more
of the men and 9 percent more of the women who have ever smoked
cigarettes than by those who have never smoked. cigarettes. This is
especially high in those who have reported their highest consumption
rate to have ,been over two packs a day (32 percent higher for men
and 43 percent higher for women). At the lower levels of consumption
the rates reported are 21 percent and 25 percent higher for those
smoking 21-40 cigarettes per day, but only 5 percent higher for men
and 7 percent higher for women for those smoking 11-20 cigarettes per
day and only 1 percent higher for both men and women who have
never smoked more than 10 cigarettes per day. The differences are
especially marked among present smokers of more than two packs per
day whose rate of reporting three or more chronic conditions is 73
percent higher for men and 143 percent higher for women than for
those who have never smoked cigarettes.
Applying differences in prevalence rates to the entire U.S. popula-
tion 17 years of age and over yields the estimate that there are approxi-
mately 11 million more cases of chronic illness annually than there
would be if all people had the same rate of~`sickness as those who had
never smoked cigarettes. A large portion of these are accounted for by
conditions classified as "chronic bronchitis and emphysema," "heart
conditions," "peptic ulcers," and "sinusitis." All but the last of these
have previously shown substantially higher mortality rates among
cigarette smokers. Si.nusitis,`being a nonfatal condition, has not been
identified in the studies of mortality previously reported. The "heart
condition" relationship is most marked in the category "arteriosclero-
tic heart disease including coronary disease."
The age-adjusted incidence rate of acute conditions for persons who
had ever smoked was 14 percent higher among men and 21 percent
higher among women than the rates for "never smokers." However,


particular caution must be taken in interpreting the results relating
specific acute conditions to cigarette smoking because of the relatively
large sampling error connected with the estimates for the several types
of acute conditions.

Since the National Health Survey is not a prospective study, it does
not identify the rate at which various types of morbidity develop in
comparable groups of smokers and nonsmokers, but reports the recent
existence of such disability. Therefore, the tidings are much more
significant when they support relationships previously identified than
when new relationships are identified. It should not be surprising that
causes of mortality which are associated with cigarette smoking have
a counterpart in disease or disability associated with smoking.
As the primary source of data in the United States on disability,
the Survey report, being based on a national probability sample,
provides a solid base for estimating the excess overall disability asso-
ciated with cigarette smoking.

HIGHLIGHTS OF CTJRRENT INFORMATION ON OVERALL
        MORTALITY AND MORBIDITY

1. The previous conclusions with respect to the association between
smoking and mortality are both confirmed and strengthened by the
recent reports. The added period of followup and analysis of deaths
of nonrespondents as well as of respondents in the Darn Study sug-
gests that the earlier reports may have understated the relationship.
2. More information is now available for specific age groups than
previously. A comparison of three ways of measuring the relationship
indicates that cigarette smoking is most important among men aged
45 to 54 both in terms of mortality ratios and exc855 deaths expressed
as a percentage of total deaths. Nevertheless, although both of these
measures decline with advancing age, the increment added to the
death rate, which reflects one's personal chances of !being affected,
continues to increase with age. For men between the ages of 35 and 59,
the excess deaths among currant cigarette smokers account for one
out of every three deaths at these ages. For women, with their lower
overall exposure to cigarettes, the comparable figure is about one
death out of every 14 at ages 35 to 59.
3. Women who smoke cigarettes show significantly elevated death
rates over those who have never smoked regularly. The magnitude
of the relationship varies with several measures of dosage. By and
large the same overall relationships between smoking and mortality
are observed for women as had previously been reported for men, but
at a lower level. Not only are the death rates for men who have never
smoked regularly higher than those for women who have never smoked

27l-3a4o-674                           23


regularly, but the effect of smoking as measured either by differences
in death rates or by mortality ratios is greater for men than for
women. At least part of this can be accounted for by the lower ex-
posure of female cigar&e smokers whether measumd by number of
cigarettes, duration of smoking, or degree of inhalation.
4. Previous .&dings on the lower death rates among those who
have discontinued cigarette smoking are confirmed and strengthened
by the additional data reviewed. Kahn's analysis of ex-smokers in the
U.S. veterans study-controlling for age at which they began smoking,
amount smoked, and current age-reveals a downward trend in risk
relative to those who continued to smoke as the duration of time dis-
continued increases. The British physician study, in which a downward
trend is reported in lung cancer death ra&s for the entire group
(smokers, ex-smokers, and those who never smoked, combined) along
with a very sharp reduction in cigarette smoking by the physiciaus, is
the beet available example of a controlled cessation experiment with
reduction of risks resulting from reduction of smoking. The Sndings
of this report support the view that epidemiological data showing
lower death rates among former smokers than among continuing
smokers cannot ,be dismissed as due to selective bias and that the bene-
fits of giving up smoking have probably been understated.
5. Cigarette smokers `have higher rates of disability than non-
smokers, whether measured by days lost from work among the em-
ployed population, by days spent ill in bed, or by the most general
measurdays of "restricted activity" due to illness or injury. Data
from the National Health Survey provide abase for estimating that in
1 year in the United States an additional 77 million man-days were
lost from work, an additional 88 million man-days were spent ill in
bed, and an additional 306 million man-days of restricted activity were
experienced *because cigarette smokers have hiiher disability rates than
nonsmokers. For men age 45 to 64,28 percent of the disability days
experienced represent the excess associated with cigarette smoking.

24


Smoking and Cardiovascular Diseases

   CONOLUkUONS OF m &JRGlWN G- `8 1964 Rnronr
Male cigarette smokers have a higher death- rate from coronary
artcry disesse than nonsmoking males, but it IS not clear that the
association has causal sign&ance.

CURRENT INMIRMATION, 1967

Important additional epidemiological information from five pros-
pective mortality studies confirms that cigarette smokers have sub-
stantially higher death rates from coronary heart disesse `than do
nonsmokers. This is true for `both men and women although the
relationships are less marked in women. Cigarette smoking also
markedly increases an individual's susceptibility to earlier death from
coronary disease. In general, mortality rates increase with increasing
amounts emoked.

Cessation of cigarette smoking is followed by a reduction in the
risk of coronary heart disease mortality relative to those who con-
tinued to smoke. Epidemiological evidence indicates that there is
little risk of coronary heart disease associated with cigar and/or pipe
smoking.
The Surgeon General's 1964 Report indicated a median mortality
ratio of 1.7 for current cigarette smokers, with a range from 1.5 to 2.0.
Additional evidence from the Hammond study (11) indicates that
young smokers between the sges of 45 and 54 have the highest mortality
ratios-three times as great for men, and twice as great for women if
they smoke 10 or more cigarettes per day, as compared with non-
smokers. In general, the mortality ratio shows the most marked in-
creases with increasing amount smoked for the ages under 65. While
the cigarette smokers older than 65 have lower mortality ratios than
those under 65, the public health significance of the relationship in
the older population is subetantial because of the large numbers of
people over 65 who die of coronary heart disease. Studies of U.S.
veterans (18)) Canadian pensioners (1)) British physicians (8,9, JO),

25


and California long&oremen (3) also provide extensive additional
information about coronary heart disease in male cigarette smokeIs ss
ccunpared to nonsmokers, supporting the above statements as they
pertain to men.

The study of British physicians (8, 9, 10) suggests that #male
cigarette smokers have the largest increase in risk for death certi-
fied to coronary thromboeis-a subcategory of coronary heart disease
describing acute coronary events,, frequently occlusive, causing rnp
cardid infarction. For that subcategory, the mortality ratio is also
largest for the younger age groups 35-54.

Prospective morbidity studies confirm the relationships between
cigarette smoking and carom-q heart disesse. These studies also
provide the opportunity to evaluate the effect of smoking independ-
ently and in combination with other known %sk factors," such as
high blood ~RW.UW and high serum cholesterol that are also impor-
tant in the pathogen&s of coronary heart disease. It has been demon-
strated that cigarette smoking not only operates as an independent
"risk factor" but that it may combine with other "risk factors" to pro
dues even greater effects on cardiovascular health.
Other types of evidence have also been presented to con&m the
epidemiologic evidence. Autopsy studies show the& cigarette smokers
have a much greater frequency of advanced coronary arteriosclerosis
than do nonsmokers. Clinical and experimental studies demonstrate
that smoking produces abnormalities of cardiovascular physiology
that may help to explain the mechanisms of haw, smoking may pro-
duce earlier death from coronary heart disease.

Human and experimental studies indicate that the nicotine ab-
sorbed from smoking may cause an increase in the myocardial tissue
demand for oxygen yet at the same time the carbon monoxide absorbed
from smoking may cause a decrease in the supply of available oxygen
from the blood necessary to meet the increased myocardial tissue
demand. Studies indicate that some persons who already have pre-
existing Coronary heart d&ease, not necessarily clinically obvious,
may be especially susceptible to the adverse physiological effects of
smoking. Evidence also indicates that important differences may
exist ,betwwn normal individuals and those with coronary heart dis-
ease in their ability to increase coronary Iblood flow to compensate for
increased myocardial tissue oxygen demand. Smoking apparxmtly can
accelerate thrombus formation of human blood, suggesting another
possible mechanism whereby smoking might increase the mortality
from coronary heart disease, especially those acute coronary events
certihd as "coronary thrombosis."
The convergence of many typee of eviden~pidemiological, ex-
perimental, pathological, and clinical-strongly suggests that ciga-
rette smoking can cause death from coronary heart disease. These

26


biomechanisms may help to explain why cigarette smokers have such
au increased risk of developing coronary !heart disease and of dying
from it.
An increasing amount of evidence has been accumulated in the past
few years relating the development of clinical cerebrovascular disease
to cigarette smoking. Most of this information has come from mor-
tality studies (17, 18), both retrospeotive and prospective, which
show that Iboth male and female smokers of cigarettes under the age
of 75, as compared to nonsmokers, have higher death rates from cere-
brovdar disease designated as the underlying cause of death on
their death certificates. This may abe especially true for younger ciga-
rette smokers age 45-54 where males had death rates about 50 percent
bigher than nonsmoking males, and females had deathrates about 100
percent ,higher than nonsmoking females. Under age 75, mortality
ratios for stroke ~increase as the number of cigarettes smoked increases.
No association has been shown for those aged 75 and over.
The new epidemiological evidence, then, indicates that cigarette
smoking may be more closely associated with cerebrovascular disease
than previously indicated in the population between the ages of 45 and
74 years. If cerebrovascular thrombosis (thrombotic brain infarction)
accounts for this association, it is possible that some of the considera-
tions of how cigarette smoking may produce coronary ththrombosis also
apply to the pathogenesis of cerebrovascular disease. Further research
is essential to understand the relationships which exist between ciga-
rette smoking and cerebrovascular disease
Additional epidemiological evidence from prospective mortality
studies provides confirmation that cigarette smoking is associated with
increttsed death rates from aortic aneurysm (nonsyphilitic), for both
men and women. In one study of male smokers an increase in death
rates was noted with increases in amount smoked.

HIGHLIGHTS OF CURRENT INFORMATION

1. Additional evidence not only confirms the fact that cigarette
smokers have increased death rates from coronary heart disease, but
also suggests how these deaths may be caused by cigarette smoking.
There is an incressing convergence of many types of evidence concern-
ing cigarette smoking and coronary heart disease which strongly sug-
gests that cigarette smoking can cause death from coronary heart
disease.

2. Cigarette smoking males have a higher coronary heart disease
death rate than nonsmoking males. `This death rate may, on the aver-
age, be 70 percent greater, and, in some, even 200 percent greater or


more in the presence of other known "risk factors" for coronary heart
disease. Female cigarette smokers also have higher coronary heart
disease death rates than do nonsmoking females, although not ss high
as that for males. In #general, the death rates from this disease increase
with amounts smoked. Cessation of cigarette smoking is followed by
a reduction in the risk of dying from coronary heart disease when
compared with the risk incurred by those who continue to smoke.
3. A greater frequency of advanced coronary arteri~lerosis is
noted in male cigarette smokers, especially in those who smoke heavily.
4. Additional evidence strengthens the association between cigarette
smoking and ~rebrovascular disesse, and suggests that some of the
pathogenetic considerations pertinent to coronary heart disease may
also apply to cerebrovascular disease.

28


Smoking and Chronic Bronchopulmonary
Diseases (Non-Neoplastic)

CONCLUSIONS OF THE SURCIEON GENERAL'S 1964 REPORT

1. Cigarette smoking is the most important of the causes of chronic
bronchitis in the United States, and increases the risk of dying from
chronic bronchitis.

2. A relationship exists between pulmonary emphysema and cig-
arette smoking but it has not been established that the relationship IS
causal. The smoking of cigarettes is associated with an increased risk
of dying from pulmonary emphysema.
3. For the bulk of the population of the United States, the impor-
tance of cigarette smoking as a cause of chronic bronchopulmonary
disease is much greater than that of atmospheric pollution or occupa-
tional exposures.

4. Cough, sputum production, or the two combined are consistently
more frequent among cigarette smokers lthan among nonsmokers.
5. Cigarette smvkmg IS associated with a reduction in ventilatory
function. Among males, cigarette smokers have a greater prevalence
of breathlessness than nonsmokers.
6. Cigarette smoking does not ap ear to cause asthma.
7. Although death certification Ii
                            s ows that cigarette smokers have
a moderately increased risk of death from influenza and pneumonia,
an association of cigarette smoking and infectious diseases is not other-
wise substantiated.

CURRENT INFORMATION, 1967

Additional evidence from the four major prospective studies indi-
cates that cigarette smokers have a markedly increased risk of dying
from chronic bronchitis and pulmonary emphysema. The range of
risk varies for cigarette smokers between three and 20 times the mor-
tality rates for nonsmokers, and depends in part on the total amount
smoked and the age group studied. Female cigarette smokers have
similarly increased mortality risks although somewhat lower than
those for males. Cessation of cigarette smoking is followed by a lower
mortality risk relative to those who continue to smoke. Generally, pipe


and cigar smokers are much less affected than cigarette smokers by
tha3ediseases.

Problems of nomenclature and diagnosis make satisfactory differ-
entiation of chronic bronchitis from pulmonary emphysema di&ult
when considering the epidemiologic data. Nevertheless autopsy studies
support the relationship between smoking and motiity. In addition,
recent information from morbidity studies indicates that smoking is
associated with symptoms of ch.i~Cc bronchopulmonary disease. Even
relatively young cigarette smokers show increased respiratory symp-
toms and decreased ventilatory funation. Cessativn of smoking is
usually followed by improvement of these characteristics. Although
some individuals may have an increased susceptibility to respiratory
disease, studies of twin-pairs in Sweden (& 6,6, I.,$)-in which one
twin is a smoker and the other is not-show that those who smoke have
a much greater frequency of respiratory symptoms and abnormalities
of ventilatory function than do their nonsmoking twins. This dem-
onstrates that cigarette smoking is of greater importance than hered-
itary and constitutional factors in the pathogenesis of chronic bron-
chopulmonary disease. Similarly, occupational exposures and air
pollution may also cause respiratory disease, but cigarette smoking is
of much greater importance.
Additional cliical and experimental laboratory evidence confirms
the fact that constituents in tobacco smoke are harmful to the bronchial
mucosa of the respiratory tract. Bronchial changes have been produced
in experimental animals exposed to cigarette smoke.

It is suspected that smoking has a direct toxic effect upon the alveo-
lar tissue of human lungs, in which case this effect might be important
in the pathogenesis of many though not all cases of human pulmonary
emphysema. Additional indirect evidence exists to substantiate this
suspected toxic effect, but additional research is needed to confbm or
deny the presence of the effect. However, the presently available evi-
dence (epidemiologic al, clinical, pathological, and experimental)
strongly suggests that cigarette smoking may well play an important
pathogenic role in many, although not necessarily all, cases of pul-
monary emphysema. The fact that other causes of pulmonary emphy-
sema exist does not detract from the validity of this inference.
Additional evidence strongly supports the conclusion in the Surgeon
General's 1964 Report that cigarette smoking is the most important of
the causes of chronic bronchitis in the United States, and increases
the risk of dying from chronic bronchitis.

30


HIGHLIGHTS OF CURRENT INFORMATION

1. New data confirm and to some extent strengthen the conclu-
siom of the Surgeon General's 1964 Report.
2. Cigarette smoking is the most important of the causes of chronic
non-neoplastic bronchopulmonary diseases in the United States. It
greatly increases the risk of dying not only from both chronic bron-
chitis but also from pulmonary emphysema.
3. C!essa$ion of smoking is followed by a reduction in mortality from
chronic bronchopulmonary disease relative to the mortality of those
who continue to smoke.
4. Even relatively young cigarette smokers frequently have demon-
strable respiratory symptoms and reduction in ventilatory function.

31


Smoking and Cancer

CONCLUSIONS OF TEE SUIWEON GENER~L'S 1964 Rnronr

1. Cigarette smoking is causally related to lung csncer in men; the
magnitude of the effect of cigarette smoking far outweighs all other
factors. The data for women, though less extensive, point in the same
direction.
2. The risk of developin
smoking and the number o P lung cancer increases with duration of
           cigarettes smoked per day, and is dimin-
ishedb discontinuin smoking.
3. Tie risk of deve(iop'
       Yc cancer of the lun
of pipe smokers, cigar smo ers, and pipe an cf for the combined group
                 cigar smokers is greater

than for nonsmokersl but much less than for cmette smokers.
The data are insufficmnt to warrant a conclusion for each group
individually.
Oral Cancer

1. The causal relationship of the smoking of pipes to the develop-
ment of cancer of the lip appears to be estabhshed.
2. Although there are suggestions of relationships between cancer
of other specific sites of the oral cavity and the several forms of tobacco
use, their causal implications cannot at present be stated.
Layngeid Cancer

Evaluation of the evidence leads to the judgment that c&ret-b
smoking is a significant factor in the causation of laryngeal cancer in
the male.
h'eophaged can&r

The evidence on the tobacco-esopha eal cancer relationship supports
the belief that an association exists. %I owvever, the data are not ade-
quate to decide whether the relationship is causal.
Cancer of Urinmy Bladder
Available data suggest an association between cigarette smoking
and urinary bladder cancer in the male but a? not s$clent to sup-
port judgment on the causal significance of this association.
Btmch ca;ncer

No relationship has been established between tobacco use and
stomach cancer.

33


CURRENT INFORMATION, 1967

Additional chemical, experimental, pathological, and epidemiolog-
ical evidence has been reported that substantiates the conclusions of
the Surgeon General's 1964 Report concerning the various sites of
cancer that were shown to be associated with or caused by smoking.

LUND CANCER

Deaths from lung cancer in the United States are continuing to rise
rapidly. Epidemiological evidence concerning cigarette smoking and
lung cancer has confirmed positive relationships with increasing num-
bers of cigarettes smoked, with increasing duration, and with deem-
ing age of initiation of the habit. Male cigarette smokers of less than
one pack a day have mortality ratios as high as 10 and smokers of more
than one pack a day have mortality ratios as high as 30.
There is a much smaller increase of the lung cancer death rates
associated with pipe and/or cigar smoking than with cigarette
smoking.

Additional evidence provides specific information on the increased
mortality ratios of female cigarette smokers. T~CW have significantly
elevated mortality ratios ranging as high as 5 for the groups with
greatest exposure. Lung cancer rates appear to be somewhat lower
for women who have never smoked regularly t.han for men who have
never smoked regularly. The mortality rates for women who smoke,
although significantly higher than for nonsmokers, are lower than
for men who smoke. How much of this is due to lower exposure to
cigarettes and how much to other factors cannot be determined from
the data available.

Ex-cigarette smokers are shown to have significantly lower death
rates compared with those who continue to smoke. As discussed under
the general topic of cessation earlier in this report, the finding of re-
duced lung cancer rates in the population of British physicians (8,9,
10) over a period of time in which t.he proportion of cigarette smokers
was dropping significantly can be interpreted as similar to a con-
trolled cessation experiment and provides critical confirmation of the
judgment that cigarette smoking is the major cause of lung cancer
and that sharp reductions can occur in the risk from lung cancer with
the cessation of smoking.

Additional information is available concerning the presence of
known or suspected carcinogens in tobacco smoke. It has been reported
that the %ar" and nicotine content of cigarette smoke* tends to reflect
the tumorigenicity of this smoke, and that a reduction of the "tar" and

* The pluase I6 YmJ and nicotine" is nsed! here as a general indicator oi total
parthlata matter fn cigar&t.e smoke.

34


nicotine content is accompanied by a reduction in the tumor&@&y.
Research is needed to identify and separate the tumor-initiating and
tumor-promoting agents in tobacco smoke and to elucidate their inter-
actions in the pathogenesis of cancer. Similarly, while additional data
are available concerning experimental carcinogen&s, it is not yet
certain that the typical characteristics of human squamous-cell lung
cancer, with invasion and metastasis, have been experimentally pro-
duced by t.obacco smoke in animals. It should be noted that this may
never be achieved not only because it may not be possible to duplicate
man's smoking action for anatomic and physiologic reasons but also
because of species' differences in cellular response.

There is evidence that certain other exposures, for example, occupa-
tional exposures to asbestos and uranium ore may interact with the
cigarette effect to produce an enhancement of the tumor-producing
effect. There is also information to indicate that the occurrence of
second primary lung cancers in smokers may be more frequent than
previously indicated.

ORAL CANCER

Substantial mortality ratios are found for cancers of the buccal
cavity and pharynx. Mortality ratios for cancer of the pharynx are
especially high. There is some evidence implicating alcohol and/or
dietary deficiencies in some of these sites. With the exception of the
pipe-lip cancer relations there are too few cases related to the indi-.
vidual parts of the buccal cavity to evaluate each independently, and
data are inadequate on the interaction of smoking with other factors.
Although all forms of smoking have high mortality ratios with these
sites, mortality ratios for those smoking cigarettes appear to be some-
what higher than for those smoking pipes and cigars, especially in
t.he case of cancer of the pharynx.

LARYNGEAL CANCER

Continued evidence from the prospective studies supports the exist-
ence of a high laryngeal cancer mortality ratio for pipe and cigar
smokers as well as for cigarette smokers. Data on the smoking habits
of patients treated for buccal cancer subsequent to their therapy sug-
gests that continuing to smoke after therapy may increase the likeli-
hood of an independent laryngeal cancer. The epidemiological evi-
dence supports the previous conclusion that cigarette smoking is a
significant factor in the causation of cancer of the larynx.

ESOPHAGEAL CANCER

Additional data from the prospective studies confirm the high
mortality ratio previously found for smokers of all forms of tobacco.

35


Aubpsy studies of smokers compared with nonsmokers speoiflcally
observingpathologicalchaqzsinesopbagealtissue havebeenreported
from both smokers and nonsmokers who died from causes other than
esophageal cancer. The findings were similar to the abnormalities
generally accepted as representing premalignant tissue changes of
the epithelium of the rv+ratory tract; that is, epithelial cells with
atypical nuclei were found far more frequently in cigarette smokers
than in nonsmokers. Tissue sections with basal cell hyperplasia were
also found more frequently in cigarette smokers and, as with the
atypical nuclei, these findings increased with amount of cigarette
smoking. Additional data to evaluate the relative importance of smok-
ing and alcohol, independently and jointly, would help clarify the
significance of these fIndings.

The Darn (19) and the Hammond (11) studies both show mortality
ratios over 2.0 for smokers of over 20 cigarettes a day, but the Doll-Hill
study (8,9), based on only 38 deaths, shows no apparent relation-
ship. Two retrospective studies have shown signi6cantly higher pro-
portions of smokers among patients than among controls. Small scale
metabolic studies suggest that cigarette smoking may block the normal
metabolism of tryptophan, which would lead to the accumulation of
carcinogenic metabolites in the urine. Further studies to verify this
finding and studies analyzing changes in the bladder tissue of smokers
as compared with nonsmokers would be helpful in arriving at a judg-
ment of the significance of the elevated death rates found in smokers
in the largest of the prospective studies.

Smsuxx AND PLLNCBEBTIC CANOEE

Epidemiological evidence does not show a sign&ant relationship
between smoking and stomach cancer. An association between ciga-
rette smoking and pancreatic cancer is implied, but the signi5cance
of this association is not clear at the present t.ime.

HIGHLIGHTS OF CURRENT INFOBMATION

LUNG CANCER

1. Additional epidemiological, pathological, and experimental data
not only confirm the conclusions of the Surgeon General's 1964 Report
regarding lung cancer in men but strengthen the causal relationship
of smoking to lung cancer in women.

36


9. Cessation of cigarette smoking sharply reduces the risk of dying
from lung cancer relative to the risk of those who continue.
3. Although additional experimental studies substantiate previous
experimental data, additional research is needed to specify the tumor-
initiating and tumor-promoting agents in tobacco smoke and to elu-
cidate the basic mechanisms of the pathogenesis of lung cancer.

LARYNGEAL CANCER

The conclusion of the Surgeon General's 1964 Report that cigarette
smoking is a significant factor in t,he causation of laryngeal cancer in
the male is supported by additional epidemiological evidence.

OTHER CANCERS

Additional evidence supports the conclusions of the Surgeon Gen-
CIY#S 1964 Report and indicates a strong association between various
forms of smoking and cancers of the buccal cavity, pharynx, and
esophagus. In the absence of further information concerning the in-
teraction of smoking with other factors known or suspected as causa-
tive agents, further conclusions cannot be made at this time, although
:L causative relationship seems likely.
Additional epidemiological, clinical, and experimental data
strengthen the association between cigarette smoking and cancer ,of
t.he urinary bladder, but the presently available data are insudcient
to infer that the relationship is ~causal.

37


Other Conditions and Areas of Research

CONCLUSIONS OFTHESUBOEON GENERAL'S 1964 REKSZT

Epidemiological studies indicate an association between cigarette
smoking and peptic ulcer which is greater for gastric than for duodenal

ulcer.
Tobacco AmbZyopia

Tobacco amblyo
lesion] has been re ated to pipe and
     P
gions. The association has not
or experimental studies.
Cirrhosis of tice Liver

Increased mortality of smokers from cirrhosis of the liver has been
shown in the prospective studies. The data are not sufficient to support
a direct or casual association.

Women who smoke cigarettes during pregnancy tend to have babies
of lower birth weight. Information is lacking on the mechanism
by which this decrease in birth wei
whether this decrease in birth weig 1 ht is produced. It is not kuown
                        t
logical fitness of &he newborn.     has any influence on the bio-

Psycho8ocid kbpects

The overwhelming evidence points to the conclusion that smoking-
its beginning, habituation, and occasional discontinuation-is to a
large extent psychologically and socially determined. This does not
rule out physiological factors, especially m respect to habituation, nor
the existence of predisposing constitutional or heredity factors.

CURRENT INFORMATION, 1967

By and large the contributions to knowledge in thii area of varied
considerations ,have been meager, although a number of investigations

27ll-3s4o-674

39


on one or another aspect of &he problem of smoking and varied health
consequences have been undertaken.

PIWTIC ULQm

The relationship between cigarette smoking and death rates from
peptic ulcer, especially gastric ulcer, is confirmed. In addition, mor-
bidity data suggest a similar relationship exists with the prevalence
of reported disease from this cause.

TOBACCO ~BLYOPIA

Tobacco amblyopia is now believed to be a manifestation of nutri-
tional amblyopia, which is aggravated by the inhalation of tobacco
smoke. Various vitamin B faotor deficiencies may be involved and
there is evidence to,suggest that chronic low vitamin B1* levels may
potentiate the toxic effects of cyanide in tobacco smoke.

CkRRHOSIS OF THE LIVER

Increased mortality of smokers from cirrhosis of the liver is found
in the prospective studies. This has generally been thought to be
largely secondary to an association between smoking and heavy con-
sumption of alcohol. Published data are inadequate to test this
interpretation.

MATERNAL SBIOEINO AND INFANT BIRTH WEIGHT

F'urther studies have confirmed the fact that women who smoke
during pregnancy `tend to have babies of lower birth weight, but
data are lacking to determine either the mechanism or the significance
of this findii.
            PSYCHOSOCIAL ASPECTS

There has been a sharp increase in the attention devoted to be-
havioral research since the Surgeon General's Report. A number of
new concepts have been developed and more sophisticated multivariate
approaches are being used. However, because of the recency of these
studies very little in the way of findings has been published on which
firm conclusions may be based.

40


Cited References

(1) BIT, E. W. It. A Canadian study of smolcing and health. Ottawa, De
  partment of National Health and Welfare, 1966.137 pp.
(2) BOOK, F. cf., hfooaa, (3. E., CLasK, P. C. ckrcinogenic aeHvity of dgarette
  smoke condensate. III. Biological activity of refined tar from several
  Q~S of cigarettes. Journal of the National Cancer Institute (Washing-
  ton) 34 (4) : 481-493, April 1965.

(3) BOBHAIV& N. 0.. HECETBB, H. H., BBIEBU)W, R. Report of a l&year foBowup
  study of the San Francisco longshoremen. Mortality from coronary heart
   disease and from all causes. Journal of Chronic Diseases (St. Louis) 16 :
   x251-x%8,1983.

(4) CEDE&WF, R. Urban factor and prevalence. of respiratory symptoms and
  "angina pectoris." A study of 9,188 twin pairs with the aid of mailed
  questionnaires. Archives of Environmental Health (Chicago) 13(6) :
   743-748, December 1968.

(5) CEDEBLOF, R., Fmumo, L., Jonsson, E., KAIJ, L. Morbidity among monzy-
  gotic twins. Archives of Environmental Health (Chicago) lO(2) : 348-3S0,
   February 1965.

(6) OEDEELOF, R., Finnuae, L., Joua~ou, E., EALT, L. Respiratory symptoms
   and %ngina pectoris" in twins with reference to smoldng habits. An
   epidemiological study with mailed questionnaire. Archives of Environ-.
   mental Health (Chicago) 13(6) : 728-737, December IQ@.
(7) DOLL, R. Cancer bronchique et tabac. Branches (Paris) M(6) : 313-324,
   Septembetitober 1966.

(8) DOLL, R., HILL, A. B. Mortality in relation to smoking: 10 years' observa-
   tions of British doctors. (Part 1.) British Medical Journal (London)
   l(53Q5) : 13QQ-1410, May 30.1964.

(9) DOLL, R., HI& A. B. Mortality in relation to smoking: 10 years' observa-
  tions of British doctors. (Concluded. ) British Medkal Journal (London)
   l(5306) : 148Ql48'7, June 6,19&L

(16) DOLL, R., HILL, A. B. Mortality of British doctors in relation to smoFsing:
   observations on coronary thrombosis. I*: Haenszel, W., editor. Epi-
   demiological Approaches to the Study of Cancer and Other Chronic
   Diseases. Bethesda, U.S. Public Health Service, National Cancer Insti-
   tute monograph No. 19, January 1966. pp. 2Q6-288.

(11) HAMxouo, E. C. Smoking in relation to the death rates of 1 million men
   and women. In: Haenssel, W., editor. Epidemiological Approaches to the
   Study of Cancer and Other Diseases. Bethesda, U.S. Public Health Service,
   National Cancer Institute monograph No. 19, January lQ38. Pp. 127-204.
(12) HOG. D., I~arm, F., WAI~~BOW, 5. Dosage patterns of cigarette smoking
   in American adults. American Journal of Public Health and the Nation's
   Health: [Inpress].
(16) mnu. H. A. The Dom study of smoking and mortality among U.S. veterans :
   report on 8?4 Years of observation. In: Haensel, W., editor. Epidemiologi-
   Cal Approaches to the Study of Cancer and Other Diseases. Bethesda,
  B.S. Pubhe Health Service, National Cancer Institute monograph No. 19,
   January 1968. Pp. l-125.

41


(Id). L~~~DLIAX, T. Smoking in relation to coronary heart disease and lnng funs.
   tion in twina A cotwin control study. Acta Medica ScandinaVCa (Stock-
   holm) 130 (eupplement 455) : 1-75.1353.
(IS) U.S. Puanro H~AL.TE Sx~vrc!x Smoking and health. Report of the Advisory
   Committee to the Surgeon General of the Public Health Service (Wash-
   ington). U.S. Department of Health, Education, and Welfare, Public
   Health Service publication No. ll03,1364.337 pp.
(16) U.S. PUBIZO HEALTH SE~BVICE. National Center for Health StatiStiC8. Cigar-
   ette emoking and health characteristics, United Stattee July I.364 to Juue
   1365. Washington, U.S. Department of Health, Education, and Welfare,
   Vital and Health Statistics series 10, No. 34, Public Health Service
   publication No. 1000, May 1967.64 pp.
(17) U.S. PDBLIO HEATH Suavxox, National Center for Health Statistics.
   Mortality from diseases associated with smoking : United States, 1950-34.
   Washington, U.S. Department of Health, Education, and Welfare, Vital
   and Health Statistics series 20, No. 4, Public Health Service publication
   No. 1000,Cctober 1036.45 pp.
(IS) U.S. Puma HEALTH Sxavxox. National Center for Health Statistics. Mor-
   tality-trends in the United States : 105MB. Washington, U.S. Department
   of Health, Education, and Welfare, Vital and Health Statistics series 20,
   No. 2, Public Health Service publication No. 1000, June 1936. 57 pp.
(19) U.S. PUBLIC HEALTH Sxuvrox. National Clearinghouse for Smoking and
   Health. Smoking and health bibliography, cumulation 1937 [in press].
   523 PP.
(90) WYXD~B, E. L., HOFFMIUPR, D. Experimental aspects of tobacco carcino-
   genesis. Diseases of the Chest. (Chicago) 44(4) : 337-344. October 1333.


PART II

  Technical Reports on the
   Relationship of Smoking
to Specific Disease Categories


CHAPTER 1

               CONTENTS

                                                  -P
Smoking and Coronary Heart Disease. .....................   47
Coronary Heart Disease Mortality. ...................   47
Coronary Heart Disease Morbidity. ...................    53
  Manifestations of Coronary Heart Disease ..............    58
  Cardiovascular Response to Smoking and/or Nicotine. ...    60
   Coronary Blood Flow in Normal Subjects. .........    60
   Coronary Blood Flow in Subjects With Coronary
      Heart Disease. ................................    61
    Carbon Monoxide Effect. ........................  62
    Studies on In V&o Thrombus Formation. .........    64
  Autopsy Studies .....................................    65
Smoking and Cerebrovascular Disease. ....................    66
Smoking and Aortic Aneurysm. ...........................    69
CitedReferences ........................................    69
Supplemental References. . . . . . . . . . . . . . . . . . , . . . . . . . . . . . . . .    76

Smoking and Cardiovascular Diseases

45


SMOKING AND CORONARY HEART DISEASE

CORONARY HEART DISEAEE MORTALITP l

The relative importance of the association between cigarette smoking
and coronary heart disease (CHD) as compared to the association of
smoking with other diseases was previously described in the introduc-
tion to chapter 11 of the Surgeon General's 1964 Report.
In the United States more persons die from coronary heart disease
than from any other single cause; and this most common form of fatal
cardiovascular disease accounts for a greater percentage of excess
deaths among cigarette smokers than do deaths from lung cancer. In
1964, there were 1,798,OOO deaths from all causes, of which almost
545,500 or 30.3 percent, were due to atherosclerotic heart disease,
including coronary heart disease. Table 1 gives the 1964 death rates for
WWNZ~ hart a?&ease per 100,000 persons by age and sex :

TABLE 1 .-196'4 death rates for coronaq heart disease per 100,000
          persons by age and sex

         All8g882544364445-6466-54    06-74     76BL     SS+
_-------___

Both Sexes_- 285.1  6.9 53.2 205.8 576.3 1,3849 2,957.7 6,882.g
Males------ 354.2 11.0 90.9 341.3 889.8 1,942.4 3,623.0 7,409.4
Females---- 218.5  3. 0 17.4 %. 3 286.4 926.5 2,464.0 6,559. 0

S008C~: N8ticUl8l Cant81 IOC HBalth StstistLa, (87).

These data illustrate &he dramatic increases in the risk of death
from coronary heart disease as age advances. For males the rates
among persons over the age of 45 appear to double from one decade
to the next; among females the increased risk of death with advancing
age is more dramatic-a threefold increase every 10 years. Of perhaps
greater importance are the relatively low death rates among females,
particularly below the age of 65, compared to males of comparable
age. The mortality differential between the sexes becomes less as age
advances ; under 45 years of age the coronary death rate among men
is five times as high as among women and in the 75-64 year age group
it is only about 1.5 times as high.

The Surgeon General's 1964 Report determined a median mortality
ratio (99) (pp. 109-110) for coronary heart disease of male current
cigarette smokers of 1.7. since this report, five large prospective

`All death ratea throughout this chapter are per 100,000 population, unless
0tJmvise indicated.                           47


studies of smoking and mortality have been updated on the basis of
longer periods of observation on each study subject. Current findings
are therefore more definitive and permit more detailed analysis of
the interrelationship of cigarette smoking to other significant variables
such as age, sex, and the nature of the smoking habit in terms of
amount and duration of smoking. Pertinent tidings are presented
below from the studies of veterans in *the United States (69) and
Canada (14) and the extensive data reported by Hammond (47)) Doll
and Hill (86, 8?6,97), and Borhani (17).

The relative excess mortality associated with cigarette smoking is
generally expressed in terms of a mortality ratio. This statistic is
defined as the ratio of the number of observed deaths among smokers,
to the expected deaths among smokers, if the age-specific mortality
rates observed among non-smokers had prevailed (69). The process
of computing the expected number of deaths among smokers takes
into account and adjusts for any differences in the age distribution
of the smokers and the nonsmokers under observation. Generally
smokers are defined as persons currently smoking cigarettes, and non-
smokers as lthose -who never smoked or who never smoked regularly.

Table 2 shows the mortality ratios for coronary heart disease deaths
among current cigarette smokers according to the amount smoked
daily in U.S. and Canadian male veterans.

TABLE 2.-Coronary hart disease morh%y ratios, age-adjwted among
    current cigarette smokers by amount smoked d&y

U.S. male veterans ______ -___- ______ -__-_
Canadian male veterans- _ _ _ _ - - _ _ _ _ _ _ _ _ _ _ _

CIgarettea smoked daily

uger   lO-3l   21-29 te   u)+

-----

1. 3   1. 7   1.8 ------ 20
1. 6   1.6 ______ 1.8 ______

Soumx: U.S. vatems stody (62) and Canadian pemstonen study (f4).

In both studies (&LX) *he mortalitiy ratios were similar and in-
creased with increasing i&ensity of cigarette smoking. Slightly higher
ratios are reported in the U.S. veterans study for current smokers of
cigarettes only.

The US. veterans study also permitted the comparison of age-
specific coronary heart disease mortality rates for ex-smokers and
current cigarette smokers (table 2A). From these data, it appears
that cessation of cigarette smoking is followed by a reduction in risk
of coronary heart disease mortality as compared to those who continue
to smoke cigarettes.


TABLE 2A.-Annuul o?eath rati per 100,000 f&n coronary he& dkeaae
by age, cigaretfe-smoking etatue and number of t$t&h sn&ed per
day, U.S. veterans study

T

T

46-M

m-64

66-74

--




--









-

Gmtmt
i!l%%

cnrrent
zzsE

Ex-
smokcm'

current
ffiZ22

~umbr smoked per day '

Ito9 ______-___-_-__    195
10to20 ________-_-__    297
21 to 39 ____-___-_-__    390
40+----------------    502

EX-
smokers '

125
133
57

._-_---.

594    432   1,374   1,105
a30    557  1,577   1,260
912    743   1,701   1,366
1,101    646   1,955   1,482

z E~~okerS who stopped for reasons other than doctor's Orders.
,$OUW.TE: U.S. veterans study (aa).

The Hammond study findings summarized in table 3 are based on
coronary heart disease deaths reported over a 4-year period among
approximately 1 million persons (441,000 men and 563,000 women).

TABLE 3 .-Coronary heart disease mortality ratios among current
    cigarette smokers only, by amount smoked oMy

-
I

Cigarattessmoked dally

Age and sex

Non-
smokers

Men:
  45to54--- ----_ -- ---___--    1.0
  55to64 _____________ - ____    1.0
  65toT4 ________ - _________    1.0
  75to84,--- ---_____-____- 1.0
Women:
  45to54 -__-_--___________ 1.0
  55to64,_-----_-----____- 1.0
  65to74 __________________ 1.0
  75to84 -_____-_______ -_--    1.0

' Exwcted deaths were leas than 10.
~~WRCIC: Hammond, E. C. (47.X

Under 10    lo-19

40+

.-

-

3.1    3.1
1.9    2. 0
1. 6     1.6
1. 4     1. 1

20
1. 6
1. 4

2. 7
20
  1. 9
____-__

  2. 4
  1. 5
  1. 3
  1. 2
0.9
  1. 3
  1. 1
- - - - - - .

   3. 4
   2. 1
(9
_ - - - _ _ - -


_-------
_-------
__-____-
- - - - - - - -

Tables 3 and 4 show that both men and women who smoke cigarettes
have relatively higher death rates from coronary heart disease than
nonsmokers, although men have higher rates than women. For each
sex and for each age group, the mortality ratios for coronary heart
disease generally increase with increased intensity of cigarette smok-
ing (table 3). The highest mortality ratios for both men and women
are observed in t.he 45-54 year age-group ; the coronary heart disease

49


death rates among heavy smokers in this age group are three times the
death rates for nonsmokers for both sexes. The mortality ratios for
both men and women decrease vith advancing age in each intensity
category. This trend may reflect the effects of selective survival of
smokers who have survived the elevated risks at younger ages of corm
onary heart disease and other diseases associated with cigareth
SIllOkiIlg.

Another explanation of the decrease in mortality ratios with aging
is that the effect of smoking, while substantial in increasing death
rates, cannot be expected to be proportionate to all other causes of
coronary heart disease as age advances. Considering the advanced de-
gree of atherosclerosis generally found among nonsmokers over age
65, the deleterious effect of smoking is more appropriately represented
by the excess in death rates among smokers. Table 4 below shows
the observed death rates from coronary heart disease among persons
studied by Hammond and classified by age, sex, and smoking status.
Although the mortality ratios decreased with age, differences in death
rates, which reflect the numbers of persons who die in each age group,
increase. This'could be interpreted to mean that, although relative to
other factors, the role of cigarette smoking tends to diminish with ad-
vancing age, the number of excess deaths per 100,000 smokers continues
to rise with advancing age.

TABLE 4.-Age-epeeiJic death raks from coronary heart disease per
    100,000 persims by age, 8ex, and smoking eta&e

Age and sex

Males:
  45to54------------------
  55to64---,--------------
  65to74--_---------------
  75tos34 _____-___-_-_____-
Females:
  45to54,__-_-----_-_____-
  55to64-----------------.
  65to74--- _______________
  75+--------------------.

1 Calcnleted from the data
Bowck: Hammond, E. C. [(/I). p. 145.1

Nonsmokers

--

422      150     272      2.8
996      542      454      1.8
2,025    1,400     625      1.5
3,871    3,132     739       1.2

66      33      33      2.0
275      163      112      1.7
941      653      288       1.4
2,349    1,973     376       1.2

The relative decrease in death rates from coronary heart disease
associated with the cessation of cigarette smoking is illustrated by
table 4A.

50


TABLE ~A.--COTO~M;GT~ heart disease (men). Age+tandardized o!eath
,'a&8 for t?X-Ci@Z?Vtte SmOkeT8 With hi&n-y Of C&re#e SmOkiTbg &y,
by former number of cigarettes smoked per day and years since last
cigarette smoking. Death rate8 for current cigarette smoker8 uith history
of cigarette smoking only ano? men who new smoked regukzriy are
8h0m fqr comparison. Men aged 60-69

                 Smoked l-19 cigarettes a day     Smoked !B+ dtpretted a day
Ep&gIretta SmOh~ (YearS
since last cigarette smokW z%   Nlllllber   Death  %z   NUCULXE  "Sh
                         ofdesths rate           of de8tba
--            -----        --        --

Under 1 year--- _-____    745    27 11,005   2,244     77  1 1,070
lt04years ____--__--  1,844     51    718 5,435     195   1,003
5togyeal-s __--------  1,770     48    725   5,803    152     732
lo+ years ___________ 4,209     84    498   8,142 205     679


    Total ex-
     smokers-----  8,569    210    635 21,624    630     813
Current cigarette
smokers- _ _ _ _- -_ _-_  22,808    781    947 56,886   1,895   1,029
Never smoked regu-
larly- _ _ _ ___ _-- _---  55,728   1,114    502 55,728   1,114     502


1 Four or mom but less than 10 deaths expected in 6ome of the component &yaar age groups.
SOURCE: Hammond, E. C. WI, p. 1481.
nolZ and EiJZ
In $5 prospective study by Doll and Hill (n) of mortality among
British physicians whose smoking habits had been previously recorded,
there were 1,369 deaths in the course of 10 years in which the underly-
ing cause was coronary heart disease ($7, table 1). The physician popu-
lation under observation totaled 320,185 person-years. The CHD
deaths were clas.si&d into three major subcategories: Group 1, com-
prising 35 CHD deaths in which an associated condition related to
smoking, e.g., lung cancer, was recorded on the death c&&ate; Group
i), comprising 721 CHD deaths in which no other significant contribu-
tory cause of death was recorded on the death certificate; and Group 3,
c&omprising 613 CHD deaths which were associated with some other
contributory cause, including conditions known to predispose t.o core-
llary heart disease, e.g., hypertension, diabetes, and obesity. The CHD
death rates for smokers and nonsmokers based only on Group 1 deaths,
while subject to large variatiofi, show the largest differentials (data not,
shown). Among smokers of 25 or more cigarettes daily, the age-ad-
justed CHD death rate was nearly eight times that in nonsmokers.
Based on Group 2 coronary heart disease deaths, presumably uncom-
l>licated by any other sign&ant disease, the mortality ratio of age-
:tdjusted death rates among continuing cigarette smokers to non-
smokers is found to be 1.6, and for heavy smokers to nonsmokers the


ratio is 2.0. However, as shown in table 5, the mortality differentials
between smokers and nonsmokers are much larger at the younger ages.

ThstE: FL-iUorta&y ralioe for di$krent typea of cormv h.mrt disease
          by mnoking h&$8
     I     Group 2 ' CHD  I      Oroup 8 CED

35 to44-------------
45to54-------------
55to64 _-___________
65to74-------------
75to84-------------
ssplua -----------___
                 -
Age adjusted-All agsa-

1.0    4.7    9.7
1.0     3.8    3.5
1.0 1.4 1.6
1.0     1.4    1.8
1.0     1.1    2.0
1.0    1.0 Q


1.0     1.6    2.0

__

1.0

-_

Cl    (`1
1.1     1.7
1.4     1.9
1.4   . .8
.a     .2
1.4   (9

1.1

.9

' Not calahbk no rata for mmamok~ because of so few deaths.
*Very few men in this category.
Souacr: Data in above table based on valups from Study of British l'by&Jans. Table 8 (27J.

The mortality ratios shown for Group 3 deaths, i.e., CHD deaths
accompanied by some other complicating disease, suggest that, for
all age groups combined, smokers do not have any special risk to
this type of coronary death. However, smokers below the age of 65
appear to be at a somewhat greater risk, while no consistent differen-
tials are observed among persons in the older age groups.

In summary, the study substantiates other mortality studies' find-
ings that CHD mortality ratios (current cigarette smokers vs. non-
smokers) increase with the number of cigarettes smoked daily, that
the ratios are highest in the age group 45-54, and that they decrease
as age advances. Moreover, smoking apparently is assooiated with
deaths from coronary heart disease among persons free of other
serious disease states.

In a prospective study of California longshoremen, Borhani (17)
reported on the mortality experience of more than 3,700 men observed
for 10 years. Table 6, derived from his data, provides some additional
insights on both the independent and the inter&ion effects of ciga-
rette smoking.

Men 45-64 years of age who were heavy smokers experienced higher
death rates from coronary heart disease than did nonsmokers independ-
ent of whether they were hypertensive or nonhypertensive.

52


TABLE 6.-Mortulitg ratio from corvnury h&W disea&?e amo?zg m&e
hypertensives and nonhypertensives by smoking history and age


    Age group           Blood ptpssarestatns 1          NOll- r
                                              -v
                                            smokus~    Emokus'
--

45 to ,rx,- _ ___ _ ____- Nonhypertenaive ______________     LO      2.2
          Hypertensive, _ _ _ _ ___ ---i ____ _     2.5      9. 6
55 to 64 ____________- Nonhypertensive -___-___------     LO      5.8
           Hypertensive, _ _ _______ __ _____     5.9      9. 4

1 Hyp&?l,S,V~ are de&& B(J tboSe b8m E$`StOlk blood m of MD mm. as. OX OV= M dbtCUc
,,,d presure of 9smm. H&T. or ov=. Nonh  &mslvfa hava ayatdlc blood praaau~ lem than 18 mm. m
cr dtastok? blood P-m @JE than 96 mm.
r~o,,smokaalnthiSpsrtiCUlSC8tUdyaFe 6fhed~SthOaen0tSmokh@8UYdW0tt=Orl~th1m~
        Y!
cigarettes per day. Smokem are those who smoke !ixl or mere pu day.
SOURCE: Borhsni, N. o., et 8l. (II).
,.n analysis was made by Schor (80) of 181 adult males who died
from coronary heart disease generally less than 2 years after receiving
;I periodic health examination. The results of this study and those of
Doll and Hill suggest that sudden death from previously undetected
coronary heart disease frequently occurs among cigarette smokers. If
tIlis is true, it may, in part, account for the small differentials in the
prevalence of coronary heart disease between smokers and nonsmokers
observed in some morbidity prevalence surveys. As will be described
in the following section, longitudinal, prospective morbidity studies
nlso show that smokers are more likely to die from sudden attacks of
coronary heart disease.
        CORONARY I&ART DISEASE MORBIDFIT*
In chapter 11 of the 1964 Surgeon General's Report, several prospec-
tive studies on the incidence of coronary heart disease (!34,31,78,88)
established that smokers were subject to higher rates than nonsmokers.
The relationship was reported to be more marked under 60 years of
age than among older persons and appeared to be associated with
myocardial infarction but not with angina pectoris. Since the 1964
report, recent findings from large-scale, on-going prospective studies
have been reported, providing additional insight on the interaction
between smoking and other important coronary heart disease risk fac-
tors. Current tidings are summarized in the following pages includ-
ing tables 7 to 13. Whenever possible, data are shown separately for
findings related to angina pectoris and those pertaining to myocardial
infarction, including sudden death attributed to coronary heart dis-
ease. Higgins (60) has drawn attention to the fact that "many factors
lnay influence or be affected by smoking habits, and obscure those
differences between smokers and nonsmokers which are directly re-
lated to the use of tobacco." In her review of the literature, Higgins
identified differences between smokers and nonsmokers in genetically
o 8lrso may include mortality data in this presentation.


determined qualities (M), in physique (77,98), in pemonality (87,
47, @, 65, 68, 69), and in social, cultural, religious, and economic
characteristics ($6, &,68,84).
Age

The elect of age on the incidence of coronary heart disease with
regard to cigarette smoking is shown in table `7 based on recent data
from the Framingham Study as yet unpublished.

TABLE 7.-ha+&imm raks and morbidity ratios for coronay be&
disease by age a& emoting status of men l%yew experience, Fm-
minghum, Maas.

AP

35to44 -_-___-_______________    1.4    4.1    2.7 T
                                      1.0 2.9
45to54 -_-__--_-________-____    4.6    11.1    0.5    1.0     2.4
55to64-----_-_---__-_____,__   16.2    25.4    9.2    1.0 1.6


SOURCE: U.8. Pnblk He&h Semke~, Fiamhgbam study (es).  (Updnted 1967)

When the incidence rate of coronary heart diseaseOamong male non-
smokers.between 35-44 yeers of age is compared with that among older
nonsmokers, the rate is seen to triple every 10 years. This marked
increase in incidence among nonsmokers reflects the effect of other
important risk factors and perhaps accounts for the decrease in mor-
bidity ratio as age advances. The independent effect of smoking on
the incidence of coronary heart disease is believed to be more appro-
priately represented by the excess morbidity rates, which increase
from 2.7 per 1,000 smokers in the age group 35-44 to 9.2 per 1,000
smokers 55-64 years of age.
&$b &!OOd ht?88WW?

Although the inhalation of cigarette smoke is frequently accom-
panied by acute transient elevations in blood pressure, habitual smok-
ers tend to have lower blood pressures than do nonsmokers (48). But,
given the presence of high blood pressure in an individual, smoking
acts as an additional risk factor for the development of coronary heart
disease (l7,$?8, m, 90,53,55,95, 96). Both the independent and the
combined effect of cigarette smoking is clearly shown in table 8 de-
rived from the experience of the Framingham and Albany studies
(W*

54


TABLE 8.-Ageadjualed morbidity ratios for coronary heart disease
among smokers and nonsmokers according to level of systolic blood
pressure

          Systolic blood p-       Noos"p;z of  "2ZJC
                                     -        ---

Under 130mm.Hg___--__------___-___--__--_--       1.0        2. 1
130 mm. Hg and over- _ _ _______________________       1.8        3.8

SOURCE: l&year Framingham and &yesr Albany experienci (3%
Eigh sem choze-stt?roz

It is not now conclusively known if cigarette smoking by itself can
cause increases in serum cholesterol. Dietary influences as well as en-
dogenous production and elimination of cholesterol must be evaluated
in greater detail with simultaneous analysis of the roles of other
risk factors, including smoking. One study of a small population of
twins in Sweden, as reported by Lundman (67)) suggests that smoking
monozygotic twins tend to have lower cholesterol levels than their
nonsmoking control twins, although the differences are not statistically
significant. Other studies suggest that smokers generally have higher
serum cholesterol than nonsmokers (13, 37, 88). However, given the
presence of high serum cholesterol, smoking increases the risk of cor-
onary heart disease (96,96').

The independent any synergistic effect of cigarette smoking is dem-
onstrated by the data in table 9 derived from the combined experience
of the Framingham and Albany studies (30).

TABLE O.-Age-adjusted morbtiity ratios for coronary heart disease
among smokers and nonsmokers according to level of serum cholesterol

Serum cholest~ol level

Low'-_-___--____________________________-----       1. 0        1. 8
Highs-___________-___________________------       2. 0        4.5

1 "Low" is below m&an. %igh" is above median value of sermn chloeaterol.
SOURCE: l&year Fmmiughcm and &year Albany esperfence (Jo).
Puzmonar/y Fzunction

The acute effects of cigarette smoking upon pulmonary function are
expressed mainly through increase in airway resistance. The dif-
ferences in pulmonary function between smokers and nonsmokers ap-
pear to be greater than can be accounted for by acute effects from a
recently smoked cigaretta (50,91). The relationship of coronary heart
disease to lowered pulmonary function as reflected by low vital capacity
and cigarette smoking is observed in the data published by the Na-

z7&SQ4 o-67--5                            55


UUU~~ msmutes oi Health based on the 12-year experience in Framing-
ham (96). Morbidity ratios derived from this publication are shown in
table 10.

TABLE lO.-Age+dju&ed mo&idiiy ratios fw coronary he& disease
among smokers and nonsmokers according to IePel of erital cupa&y

wal capacity

Under 3 liters _-_---_ - ___ ___ _ ______ _ _________ ___       1.0
      I I
3 liters or more ---------------------------------      1.7

1.7
2.4

Sonaclr: The FraxnIngham heart study. (6W).

Here again, the independent and combined effects of cigarette smok-
ing are observed.
Physical znaotivity

A physically inactive or sedentary individusl seems to run a higher
risk of developing coronary heart disesse (89, .&I, 0,76). Spain (88)
reported that, in his prospective study of 3,000 men "* * * the rela-
tionship of occupational physical activity to smoking habits revealed
that one of six sedentary workers were heavy smokers and one of five
strenuous workers were heavy smokers." Weinblatt, in reporting the
experience of the Health Insurance Plan of Greater New York (100)
also found that a higher proportion (41.9 versus 36.0 percent) of cig-
arette smokers were classified in the "most active" physical activity
category.

The independent and the combined effects between cigarette smok-
ing and physical activity are shown in table 11. The morbidity ratios
for myocardial infarctions are derived from published data.

TABLE ll.-Age-cs&justed morbidity ratios for myocardti infarctions
among smokers ano! nowmokers according to phgsi.eal act&i@ &we1

Physical actitity

Most active------------_------_---_____-____-__       1. 0
      I I
Lenstactive---------_-_-----------~-----------       24

2. 6
3. 4

SOUBCE: Weinblatt, E. (fOrI).
Socioenvironmenta.2 Stress

Since 1955, research on socioenvironmental stress in relation to cor-
onary heart disease has increased greatly (#, 99). Among the factors
studied that indicate a strong association with coronary heart disease
incidence and prevalence is sociocultural mobility, that is, moving
from one social setting to another. The interaction of this factor and

56


cigarette smoking has been reported by Syme (90,91) in both an ur-
ban and rural setting. Apparently in both areas cigarette smokers
\vere more culturally mobile than nonsmokers. The independent effect
of cigarette smoking on the incidence of coronary heart disease is
shown in the morbidity ratios in table 12 derived from the North
Dakota study (91).

TABLE 12.-Age-adjusted morbidity ratios for coronary heart d&ease
among smokers and nonsmokers according to sociocu&uraJ mobility
status

socioouItumI status

Ne;wwT$ed   Current and
     fonn~ok~grette

stable __________________ - ____ - _______________ -       1. 0
     1 1
Ijighly mobile--- __ _- ____ -__ ___ ___ _ __ _ ___ _ ___ ___       2. 3

1. 5
3. 2

SOURCE: North Dakota study. (81)
Personality Type

Various investigators have long `suspected a possible pathogenetic
role of the central nervous system in coronary heart disease (35). In
a series of reports, Rosenman (82,8@ and Jenkins (51) have described
a personality pattern or overt emotional complex which, while asso-
ciated wit.h other known risk factors, appears to predict coronary heart
disease more effectively than do other risk factors. This emotional com-
plex, `&which they have termed Behavior Pattern Type A, is composed
of an enhanced competitiveness, drive, aggressiveness and hostility,
nud an excessive sense of time urgency." Recent unpublished data based
upon prospective observation of more than 3,000 men for a 4+`&-year
period (51) discloses that smokers have a higher percentage (54 versus
4'7 percent) of type A persons among them. Moreover, the incidence of
coronary heart disease is shown to be related independently to both
smoking status and personality type. Morbidity ratios, derived from
the incidence data, are shown in table 13 which clearly demonstrates
the independent effects of cigarette smoking and its interaction with
personality characteristics.

TABLE 13.-Morbidity ratios of cigarette smokers as compared to non.-
         smokers by personality type

SOUBCE: Unpublished data from We&em Collaborative Group Study, San kandseo, C&if. (eb).


Mdtiple-R&k Factor8

The method of analysis traditionally employed by epidemiologists,
that of the comparison of rates for. multiple cross-classifications of
the data, generally requires a large study population at relatively high
incidence of significant events. Since coronary heart disease incidence
rates are low and study populations are necessarily small because of
practical and practicable limitations, definitive analysis of the inde-
pendence and interaction between risk factors have generally been re-
stricted to two factors at a time. Truett (96) applied a multiple logistic
function proposed by Cornfield to investigate the independent effect
on the incidence of coronary heart disease of seven risk factors: Age,
serum cholesterol, systolic blood pressure, relative weight, hemoglobin,
cigarettes per day, and ECG abnormalities. The method was used in the
analysis of data compiled in the Framingham study during a 12-year
period. A discriminant function coefficient was computed for each risk
factor. These-coefficients represent the relative importance of each fac-
tor with respect. to the other six factors in the development of coronary
heart disease. While theoretical considerations underlying the logistic
risk function are not fully satisfied by the actual data, the approxima-
tion given by the function to observed rates is very good.
Consequently, Truett and Cornfield believe that the present compu-
tations permit the conclusion that "the most important risk factors,
aside from age itself, are cholesterol, cigarette smoking, ECG abnor-
mality, and blood pressure" (96).

MIANIFE~TATION OF CORONARY HIURT DIEIEASE

Coronary heart disease is essentially comprised of three major mani-
festations or subcategories :
    1. Fatal myocardial infarctions, including sudden deaths attrib-
uted to coronary heart disease ;
  2. Nonfatal myocardial infarction; and
  3. Angina pectoris.

Generally, investigators in their analysis of the relationship of risk
factors to the incidence of coronary heart disease have not subdivided
the observed coronary events into the three major subcategories pri-
marily because the paucity of events in each category did not permit
definitive conclusions on any differences observed. However, the pool-
ing of data from some of the larger prospective studies holds promise
of a more complete analysis of the independent and synergistic effects
of each risk factor on each of the subcategories of coronary disease.
Findings from these analyses might provide some insights into the
underlying pathophysiological mechanisms t.hrough which a risk factor
operates. The pooled data from the Albany and Framingham studies
a.nd data from the HIP study include the observed associations of

58


cigarette smoking with each of the three major manifestations. Mor-
bidity ratios have been derived from these studies and are presented
in table 14.

TABLE 14.-Agemfjusted nwrbidity mtios for subcategories of coronary
     heart disease among smokers and nonsmokers

                        Framingham-Albany   Ewaltll fneurance plan
        Disease category             Non-  "&Tg    NOU-
                             smokem of          smokea of
                         cIgarettea         clgncettes  "m%
-        --- ~-- --- ~_
Fatal myocardid infarction _________      1. 0    2. 4      1. 0     2. 1
Non-f&d myocardial infarction- _ _ _ _     1. 0    2. 3      1. 0     1. 8
Angina pcctorie __________ ___ ___ _ ___     1. 0     1. 1      1. 0     1. 7

Socmcr: Second Report of the Comblned Erperlence from Albany and Framingbarn Studies (JO). VII-
&dished Data from Health Innuance Plan Study (loo).

The association of cigarette smoking to angina pectoris is not a con-
sistent one. A clear-cut association was found in the Health Insurance
Plan Study (ratio of 1.7) ; a similar association is also found in un-
published data from Framingham considered separately. However, no
association between cigarette smoking and the incidence of angina
pectoris was found in the Albany experience. Cederlof (19)) in his
analysis of prevalence data on angina pectoris obtained by question-
naire, found no statistically significant difference in prevalence rates
between 453 monozygotic twin pairs with dissimilar smoking habits.
In a larger study of about 9,000 persons from the twin register where
genetic factors were uncontrolled, Cederlof (19, m) did find a sig-
nificantly higher prevalence of angina pectoris among smokers than
nonsmokers, particularly in men (ratio of 1.6) (fl) .

Friedman (&!) and Epstein (96) have clearly described the in-
herent biases in prevalence studies which may lead to findings of risk
gradients that are different from those obtained in prospective inci-
dence studies. One of these limitations is that fatal cases are under-
represented in a prevalence survey. Thus, since it appears that cigar-
ette smoking is more closely related to the incidence of fatal myocar-
dial infarctions than to other forms of coronary heart disease, it is ex-
pected that morbidity ratios derived from prevalence surveys would be
lower than those computed from incidence data. With these restrictions
in mind, Russek (83) in a survey of 12,000 men in 14 occupational
groups found that the morbidity ratio of coronary heart disease preva-
lence among cigarette smokers was as high as 1.8. In contrast, in a study
of 77 identical and 89 fraternal twins in Sweden, comparing smokers
with their respective nonsmoking twins, Lundman (67) reported no
excess prevalence of overt or silent coronary heart disease. However,


the prevalence of these conditions, as Lundman concluded, "was too
low to permit of definitive conclusions."

~ARDI~~A~~~LAR RJWPONSE. m SIKOKING AND/OR NIOOT~VE

As noted in the Surgeon General's 1964 Report, nicotine has definite
physiologic effects on the cardiovascular system of experimental ani-
mals and of man. These include increases in heart rate, systemic arte-
rial pressure, cardiac output, stroke volume, and velocity of myocardial
contraction, all resulting in an increased myocardial tissue oxygen
demand (16). Coronary blood flow studies will be reported in the next
section under a separate subheading. These effects parallel those ob-
served with catecholamines (epinephrine and norepinephrine) . The
effects can be blocked by the injection of tetraethylammonium chloride
and markedly reduced by adrenalectomy (28). Nicotine has been re-
peatedly shown to release endogenous catecholamines (67,68,69, M,
Y&Z?). However, the mechanism by which nicotine affects the cardio-
vascular system is more complex than the release of catecholamines
from the adrenal medulla. Direct and indirect (via the carotid body
and other chemoreceptors) stimulation of the vasomotor center, stimu-
lation of sympathetic ganglia, release of norepinephrine from local
stores, and release of antidiuretic hormone are included among other
postulated mechanisms of action involved in nicotine's effect on the
cardiovascular system (IS, &`,85).
Coronary Blood Fho in Nod Subjects

The action of smoking and/or nicotine on the coronary blood flow
of normal human subjects has not yetbeen definitively established, but
apparently normal subjects can increase their coronary blood flows
sufhciently to maintain a compensatory blood supply to the myocar-
dium despite the increased myocardial tissue demand for oxygen
caused by cigarette smoking. Earlier findings of increased coronary
blood flow in normal men, in response to cigarette smoking (11)) were
not reproduced in a more recent study (16). In this latter study, al-
though a trend towards a slight increase in coronary blood flow was
observed in the particular normal persons studied, it was not
significant.

Direct injection of nicotine into the left coronary artery of dogs in-
der conditions of constant flow rate resulted in increased coronary VW+
cular resistance (38,S4). This response could be reduced by vagal nerve
stimulation or prior adminstration of acetylcholine; an immediate in-
crease in cardiac contractile force was also observed that could be
similarly reduced. It was concluded that these responses to nicotine
resulted from sympathetic nervous system activity or from the release
of catecholamines by myocardial chromaffin tissue (64).

Blood from the smoke-exposed lung tissue of dogs, directly perfused

60


into the coronary artery, failed to increase coronary resistance (38).
This effect was thought to be secondary to that of histamine, known
to act as a coronary vasodilator, which apparently is released from the
lung tissue of dogs during their exposure to smoke (8).

Inen blood from the smoke-exposed lung was perfused through
the systemic circulation of dogs while the coronaries were being
perfused with non-smoke-exposed blood, the typical release of cate-
cholamines occurred with many of the usual effects on cardiovascular
parameters except that the coronary vascular resistance increased
under these experimental conditions, apparently due to the increased
activity of the sympathetic nervous system (98).

Since it is well kuown that exposing dogs to cigarette smoke without
isolating and separately perfusing the coronary circulation normally
results in an increase of the coronary blood flow (38)) the manipulation
of experimental conditions as described suggests that there is a
masking effect by the catecholamines on nicotine's direct action on
the coronary circulation (38).

These studies may relate, by analogy, to humans and indicate that
smoking, in "normal" individuals, may produce at least two actions
that can affect coronary blood flow : (1) a decrease in coronary blood
flow by a possible direct action of nicotine on the coronary circulation
(demonstrated in dogs), and (2) an increase in coronary blood flow
as the usual resultant of varying responses to the intermediating
action of catecholamines and other physiologic processes (demonstrated
in both animals and humans).

Cormmy Blood Pkm in Subjects with Coronay Heart Disease

The effect of cigarette smoking on coronary blood flow was studied
in patients with coronary heart, disease (79). As was seen in normal
subjects, significant increases in heart rate,' arterial pressure, and
cardiac output were noted. In contrast to the normal individuals
studied, patients with coronary heart disease distinctly showed a
much less significant compensatory increase in their coronary blood
flows. These results were confirmed by a later study (16)) using the
Rubidium 84 method <to estimate coronary blood flow. This study also
showed that in the coronary patients studied, there was no adequate
compensatory increase in coronary blood flow to meet the increased
myocardial tissue demand for oxygen. Coronary blood flow appar-
ently decreased as a result of cigarette smoking, in this particular
study group of coronary patients. Although the decreases noted were
not marked, they were statistically significant, and indicated t,hat a
difference existed between these coronary patients as compared to the
normal subjects studied.

A difference in the coronary blood flow response to nitrogIycerine
has also been demonstrated in normal subjects compared to subjects
with coronary heart disease. This was shown in studies using t,he

61


nitrous oxide (18,&) and the Rubidium 84 (16) methods to measure
coronary blood flow. In response to nitroglycerine the normal indi-
viduals generally increased their coronary blood flow significantly,
but the coronary patients generally did not.
Anima.1 studies have also demonstrated the decreased ability of
atherosclerotic coronary arteries to increase coronary blood flow, as
compared to the coronary arteries in normal animals (94): Dogs with
`experimentally produced coronary artery insufficiency also show this
decreased ability (19). Similar differences between animals with nor-
mal coronary arteries as compared to atherosclerotic coronary arteries
have been demonstrated in response to ergonovine (80).
The above studies indicate that the effect of nicotine upon the car-
diovascular system, mediated in part by the action of released catechol-
amines, is generally to increase heart rate and cardiac output, and to
raise systemic arterial pressure temporarily. Findings concerning the
effect of nicotine on coronary blood flow are presently thought to be
largely due to the indirect effects of nicotine upon the cardiovascular
system. Other animal studies indicate that there may :be a direct action
of nicotine on the coronary vasculature to increase coronary vascular
resistance, thus tending to reduce coronary blood flow. There are no
human studies on the direct action of nicotine by itself on the coronary
vasculature; such studies, involving the direct injection of nicotine
into diseased human coronary arteries, might be dangerous. Normal
individuals apparently can increase their coronary blood flows to com-
pensate `for the increased myocardial tissue oxygen demand, but ap-
parently some patients with coronary heart disease cannot, as shown
by their response to smoking.
Thus some patients with coronary heart disease may be at a par-
ticular disadvantage when smoking and under other stresses since
their coronary arteries apparently cannot dilate to supply blood flow
adequate to meet the increased oxygen demand associated with nico-
tine-induced catecholamine release. The interaction of the above ef-
fect with recent findings concerning carbon monoxide, described in
the next section, may be especially important in those individuals with
coronary heart disease. The present studies indicate that the effect of
cigarette smoking on coronary blood flow, in the presence of pre-
existing coronary heart disease, may, in part, contribute to the in-
creased incidence of acute myocardial infarctions that have been
observed to be associated with cigarette smoking. No relationship be-
tween the smoking effect on coronary blood flow and the pathogenesis
of coronary atherosclerosis per se is presently suggested. Additional
research is needed.
Carbon Monoxide Effect
The gaseous phase of cigarette smoke contains about 4 percent car-
bon monoxide. This quantity can increase the levels of carboxyhemo-

62


globin saturation of cigarette smokers from 2 percent to 10 percent
(91). The average nonsmoker, depending on environmental exposure;
usually has less than 2 percent carboxyhemoglobin saturation (10).
Since smokers of one pack or more a day may have chronically eleva'ted
carboxyhemoglobin levels of more than 4 percent (9), there may be
appreciable differences in the carboxyhemoglobin levels between some
heavy cigarette smokers and nonsmokers.
In addition to displacing oxyhemoglobin, carbon monoxide effects a
shift in the oxygen-hemoglobin dissociation curve (9,3,4 6, 6). This
may result in a ,decreased release of oxygen at the tissue level. A series
of studies (61, 09) has been performed on young adults to analyze
the effect of cigarette smoking on carboxyhemoglobin levels, and the
consequent effect on some parameters of cardiopulmonary function.
An increased post exercise oxygen debt was observed after cigarette
smoking as compared to controls. This, in part, may reflect not only
vcntilatory disturbances but also a decreased supply of oxygen in
the blood due to the catibon monoxide effect, resulting in less available
oxygen to meet the increased tissue demand. Similar post-exercise
oxygen debts have been noted after inhalation of enough carbon
monoxide to produce comparable blood levels of carboxyhemoglobin
cw .
The consequence of the smoking/carbon monoxide effect appears to
be especially important in the myocardium where relatively more
oxygen is normally extracted from the coronary circulation as com-
pared to other organ systems. (Coronary venous .bloocl usually has
an oxygen saturation of less than 25 percent, whereas blood leaving
some other organs is about 75 percent saturated with oxygen (&).)

Dogs were exposed to carbon monoxide to elevate their carboxy-
hemoglobin saturation levels (9). In response to inhalation of carbon
monoxide there was an increase in coronary blood flow but a decrease
in coronarY arterial-venous oxygen differences. Patients with coronary
heart disease were also studied following inhalation of enough carbon
monoxide to elevate their carboxyhemoglobin saturation levels to the
range of 5 to 12 percent (9). In response to carbon monoxide there was
generally an increase in the cardiac output and the coronary blood
flow in most of the patients. While the systemic arterial-venous oxygen
differences varied, either increasing or decreasing, the coronary ar-
terial-venous oxygen differences decreased, indicating a decreased
oxygen extraction by the myocardial tissue despite the mYocardium's
increased demand for oxygen.. These decreases in myocardial oxygen
extraction tiiw related to increases in the carboxyhemoglobin &ura-
tion levels. It was observed that some patients evidently could corn--
Pensate by increasing their coronary blood flows adequately to supply
the myocardial tissue with sufficient oxygen, as indicated by a rise in
mYocardia1 oxygen uptake in these individuals. However, the other

63


patients with coronary heart disease, evidently more severe, could not
increase their coronary blood flow rate enough to compensate for the
decreased oxygen carried by the blood. This la.tter group of patie&,
even though they had increased cardiac output, had less sign&ant
increases of coronary blood tlow than those noted in the first group
of patients. .The coronary arterial-venous oxygen differences and the
myocardial tissue oxygen uptakes both decreased, indicating that the
myocardial tissue oxggen demand was not being met entirely.
The reduction in the amount of oxygen available to the myocardial
tissue caused by the absorption of carbon monoxide from tobacco
smoke may be especially critical in persons with pre-existing coronary
heart disease, especially when they cannot significantly increase coro-
nary blood flow to compensate for increased myocardial tissue oxygen
demand. The carbon monoxide effect may, in part, contribute to the
increased incidence of myocardial infarctions t.hat occur in cigarette
smokers. Additional research is needed.
i!&u&98 on In VitrO Th~0??&&8 Fmmation
Recent studies have indicated that cigarette smoking may accelerate
thrombus formation of human blood in vitro. Platelet adhesiveness,
as measured by in -vitro tests, also appears to be increased by cigarette
smoking (I, .& 71,87'). Other studies, comparing smokers with non-
smokers, indicate that the platelet survival time of the smokers is
shortened (73) and the platelet turnover rate is increased (7@. Studies
of ,animals show there is also an increased tendency for the platelets
to adhere to the vascular endothelium.
Platelet adhesiveness is reported to be increased in in vitro studies
using the Chandler rotating loop (.X$33,34) ; these studies generally
show a consistent acceleration of the rate of thrombus formation.
Other Gz vitro tests show changes in thrombus formation and some
parameters of coagulation as a result of smoking (66, 66,87). How-
ever, problems in experimental design and the multiplicity of tests
used, measuring either the same or overlapping portions of the com-
plicated coagulation process with varying results, cause difficulty in
evaluating these results (71).
The mechanism of changes in characteristics of the platelets in
smokers is being investigated, ,but there are indications that the release
of catecholamines, especially epinephrine, caused by the absorption
of nicotine during smoking may be intimately involved (71, 79). In
small doses, epinephrine has been shown to promote thrombus forma-
tion and coagulation: but in large doses it inhibits these prowsses.
Changes in the electrical charge of the platelet membrane have also
been implicated in increasing platelet adhesiveness (101) , increasing
adherence to the vascular endothelium, and accelerating thrombus
formation as measured by the Chandler loop method. Some of the
alterations in thrombus formation may be mediated by an interaction

64


with serum-free fatty acids and cholesterol (70) but current evidence
suggests that inhalation of cigarette smoke acts primarily through
other independent factors (201). Thus, cigarette smoking may cause
an acceleration of the in &ro thrombus formation of human blood. It
js reasonable to suspect that cigarette smoking, in part by a.flecting
the thrombus-forming process in human blood, may account for some
of the excess coronary ,heart disease deaths that occur in cigarette
smokers, especially some of the deaths certified as "acute coronary
thrombosis." Further research is necessary before any de&&e con-
clusion can be made.

A~OPSY STUDIES

The two most significant pathological studies of the relationship of
smoking history to atherosclerotic changes in human coronary arteries
have been reported by Auerbach and Strong. Auerbach (7) studied
1,372 males for whom a smoking history was available and who had
died of causes other than coronary heart disease. He found that the
percentage of men with an advanced degree of coronary atherosclerosis
was higher among cigarette smokers than among nonsmokers, and that
t,he percentage increased with amount of cigarette smoking. Both
nmong smokers and nonsmokers the percentage of men with advanced
coronary at.herosclerosis also increased with age. This relationship
held up even when the following were excluded: men with a history
of diabetes, men who had died of any type of heart disease, and men
whose hearts weighed 400 gm. or more. A matched set analysis was
also carried out (reincluding some diabetics and heart disease deaths)
and again the percentage of men with advanced coronary athero-
sclerosis was less among nonsmokers than among men who had been
current cigarette smokers, and this percentage increased with the
amount smoked.

Strong (89) in a study of coronary arteries from 645 autopsied
males, 20 to 64 years of age, excluded patients with diseases he
thought to be associated with smoking (emphysema, lung cancer,
etc.), or with coronary heart disease (myocardial infarction, hyper-
tension, diabetes, stroke, etc.). He found that the mean percent of
coronary intimal surface occupied by raised atherosclerotic lesions
was approximately twice as great in heavy smokers (25+ cigarettes/
day), and about one-third greater in lightsmokers (less than 25yday),
than in nonsmokers. Calcified lesions and mean coronary wall thickness
measured radiographically were on the average highest in heavy
smokers and lowest in nonsmokers. Differences among these smoking
categories were generally greatest at younger ages.

These autopsy studies suggest ,that smoking, in addition to the
acute immediate effect associated with the act of smoking, has a
chronic effect leading to advanced degrees of atherogenesis. However,
these findings may, in part, reflect the differences noted between

65


smokers (7,249)) particularly heavy smokers, and nonsmokers in regard
to greater obesity, higher dietary fat intake, and higher serum chole&
terol levels. Further analyses of autopsy series are needed to determine
the independent effects of cigarette smoking on atherogenesis.

SMOKING AND CEREBROVASCULAR DISEASE

An increasing amount of evidence has accumulated in the past few
years relating the development of clinical cerebrovascular disease to
cigarette smoking. Most of this information has come from the pros-
pective mortality studies.

Hammond has reported the following data from his *large prospec-
tive study (47)) noted in tabIe 15.

TABLE 15.-Ceree6mi ?XXS&T lesti. Age-standu~dked death r&a, b3I

type of smoking (lifetime hisib

Never smoked regularly- _ ___ ___ _ ___ _ ___
Pipe, cigar--- - --_--__ __ ___________ - ___
Cigarette and other ____________________
Cigarette only --__________________ - ____
   Total__------__-----____________

WOMEN

Never smoked regularly- _ __ _ _-__ _- ____ _     18
Cigarette- ________ - _______ - ___________     38
   Total--------__-----------------     25

MEN

Never smoked regularly- _ __ _______ -___ _   1. 00
Pipe, cigar-__-------------------------    .89
Cigarette and other- _ ___ ___ _ ______ _--_ _   1. 00
Cigarette only ____ ___ ___ _ _ _ _ ____ ___ _ ___   1. 50

WOMEN

Never smoked regularly- _ _ __ _ _ __ _ _ __ - -_   1. 00
Cigarette- _ _ _ _ _ __ _ _ _ _ - - - - __ _ _ __ _ _ _ _ _ _ _   2 11

BOWCE: E.C.Hammond(47.k

66

) &dageat8taTtof 8tudy -

          Age


4&M  -4   6674     75-M


CVL death rates per 100,000
       person-years

28
25
28
42
35

92
100
129
130
116





57
88
64

349   1, 358
369   1,371
361     990
477   1,168
391   1, 272

228   1, 082
315   1,277
238   1, 091

CVL mortality ratios

1. 00
1. 09
1. 40
1. 41





1. 00
1. 54

1. 00    1. 00
1. 06    1. 01
1. 03     .73
1. 37     .86

1. 00    1. 00
1. 38    1. 18


Between the ages of 45 and 74 the death rates from stroke for male
smokers were 37 to 50 percent higher than those for male nonsmokers of
comparable age. In female smokers the death rates from stroke were
33 to 111 percent greater than those for nonsmokers. Above the age of
74 the differences between the two groups were much less.
The data in Table 16 concerning smoking and death rates from stroke
are derived from the U.S. veterans study (62).

TABLE le.-Mortuliiy ratios and death rates for stroke as underlying
     cau.se among current smokers of cigarettes only

                          Quantity of cigarettes smoked per day
                                                 --~-

                           0      l-9     104     2l-30     40+
--                          --

Jiortality ratio (all ages) _______   1. 00    1. 51    1. 42    1. 70     1. 59
Death rates :
  Age 55 t.o64------- _______     59     92     112     125     130
  Age65 to74 ____________-_    280    323     312     382     502

SOURCE: U.S. vetem study (6.8).

When stroke was certified as the principal cause of death, the death
rates for smokers were higher than for nonsmokers; however, no pro-
nounced increase was noted in the mortality ratios as the degree of
smoking increased. The death rates from stroke for all ages was 59
percent higher in heavy smokers (40 or more cigarettes) than in
nonsmokers.

TABLE 17.-Mortality ratios and death ratee for stroke as the underlying
or contributory diagnosis among current smokers of cigarettes o&y

I      QufmtitY of dkwettes smoked per day

                           0
--

Mortality ratio (all ages) _______   1. 00
Death rates:
  Age55to 64_-----------_-    101
  Age65 to 74 ______________    424

SOURCR: U.S. veterans study (6s).

-


_-









-

.-

l-9

1.45

152
514

lO-20     21-39
~-

1.45    1. 75


  174    195
  520    616

-


--









-

u)+
---

  1. E3

2.6
724

Stroke, listed as either the underlying or contributory cause of death
on the death certificate, was also associated with progressively increas-
ing mortality ratios and death rates as the extent of smoking in-
creased. Heavy smokers here had an 83 percent greater mortality from
stroke than nonsmokers.

Mortality data #by underlying cause of death may often be mislead-
ing, particularly when stroke is concerned. Many stroke patients have

67


concomitant coronary heart disease, or may develop pneumonia and
other complications that may hasten death. The death certificate may
carry stroke as the underly*mg cause or as the contributory cause of
death, depending upon t.he interpretation of the physician at the time.
The important addition of these data is that smoking is associated
with a higher mortality from stroke, whether the stroke is recorded as
either the underlying cause or as the contributory cause of death.

These two studies indicate that smoking may be associated with a
higher mortality from stroke in the relatively younger age groups
(under age 74). More than one-half the strokes that occur each year
are in the group above age 75 and in this group there is no evidence
relating. smoking to cerebrovascular disease.

Another large study has been conducted analyzing the mortality of
50?000 former students who entered Harvard University or the Univer-
sity of Pennsylvania during the years 1916-50 (7&76,76). From this
population 171 deaths from cerebrovascular accidents have been identi-
fled. A review of the medical records from their college years has been
carried out, and selected factors were correlated with the later occur-
rence of stroke. Seven precursive "risk factors" present at the time
of college attendance have been de&red : Cigarette smoking, high blood
pressure, excess body weight, short stature, a history of early parental
death, a history of nonparticipation in college sports, and a history
of "heart consciousness'~ (also shown to be correlated with coronary
heart disease). Cigarette smoking and a history of early parental death
were more strongly correlated with thrombotic stroke than with hem-
orrhagic stroke. Students who smoked more than 10 cigarettes daily
were at twice the risk of having a fatal stroke than were those who
smoked less or not at all.

In 1965 the Framingham study (6.4) reported that while an excess
development of thrombotio brain infarction appeared to be associated
with cigarette smoking, statistically significant differences could not
be demonstrated with the small number of cases available at that time.
More recent data from Framingham indicates that cigarette smoking
increases the risk of stroke in males. The relat.ively small number of
women smokers had too few strokes for adequate analysis.

The new epidemiological evidence, then, indicates that cigarette
smoking may be more closely associated with cerebrovascular disease
in the population between the ages of 45 and 74 years than was previ-
ously indicated. If cerebrovascular thrombosis (thrombotic brain in-
farction) accounts for this association, it is possible that some of the
considerations of how cigarette smoking may produce coronary throm-
bosis also apply to the pathogenesis of cerebrovascular disease. Further
research is essential to understand the relationships that exist ,between
cigarette smoking and cerebrovascular disease.

68


SMOKING AND AORTIC ANEURYSM

Additional information on mortality data concerning aortic aneu-
rysm has been provided by the U.S. veterans study (69) and the Ham-
lnond (47) studies, as noted in tables 18 and 19, respectively.

TABLE la.-Mortalit ratios and age-standardized death rates for a.ortic
  aneurysm in U.S. veterans, current smokers of cigarettes only

Number of cigarettes smoked per day

                           0       l-9     lO-2l     21-2!3     u)+
                               --
--

&~ortality ratio ________________   1. 00    2. 12    5. 53    5. 95    7. 26


       DEATH RATES
Age:
  55to64--- _______________     6  :"7     27     43      44
  65 to 74 _____ _ ______ - _____     25          123    157     221



SOURCE: U.S. veterans study (6n.

TABLE lg.-Mortality ratios and age-standardized death rates for aortzc
                   aneurysm

Age 46-64 years

Age 66-79 years

iMortality ratio--- ____ _ ___ _ __- ___ __ _ _ __   3. 89    2. 62    3. 33    4.92
Dcathrates_____------_---------------  l4(1)   1sm  43(H)  118(Y)

* Numbers in parentheses indicate death rates of those who never smoked regularly.
SOURCE: Hammond, E. C. (47).

It is apparent that there is a close association between cigarette
smoking and death caused by aortic aneurysms.
Thus, the additional evidence confirms the previously observed asso-
ciation between cigarette smoking and death due to nonsyphilitic
aortic aneurysm.

CITED REFERENCES

(I) ASHBY, P., DALBY, A. M., MILLAB, J. H. D. Smoking and platelet stickiness.
   Lancet (London) 2 : X&-15@ July 24, 1965.
(2) ASTRUP, P. An abnormality in the oxygen-dissociation curve of blood from
  patients with Buergeis disease and patfents with nonspeciik myocar-
  d&is, Lancet (London) 2: 11621154, Nov. 28,1Q64.
(3) ASTBUP, P. Den kliniske betydning af forskydninger i oksihaemoglobinets
  dissociations-kurve. Nordisk Medicin (Stockholm) 76 : 103%lQ4l. 1968.

69


~4) AUTBUP, r. varmuoner ~0gsUmemoglohineta diy3sociationskurve. Ugeskrlft
   for Laeger (Kobenhavn) l.28(24) : 6Q5-701, 1988.
(5) ASTBTJP, P. Haemmet iltafgift fra blodet og udviklingen af oblitererende
   arteriesygdomme. Ugeskrift for Laeger (Kobenhavn) 128(24) : 701-708,
    1968.

(6) ABTBUP, P., Hm.~uxa-L~~sxx, P., EJELDSEN, K., Isfmrmd~aaB0, EC. The
   effect of tobacco smoking on the dissociation curve of oxyhemoglobia.
   Investigations in patients with occlusive arterial disease8 in normal sub-
   jects. Scandinavian Journal of Clinical and Laboratory Investigation
   (Oslo) U(4) : 450-487,1966.

(7) AUEBBACH, O., HAMYWD, E. C., Gmxvxx~, L. Smoking in relation to
   atherosclerosis of the coronary arteries. New England Journal of Medi-
   cine (Boston) 273(15) : 776-77Q, Oct. 7, 198s.
(8) Avra~o, D. M., SUAXEK, hf., Fonuu, L. E. Cardiopulmonary effects of to-
   bacco and related substances. I. The release of histamine during inhala-
   tion of cigarette smoke and anoxemia in the heart-lung and intact dog
   preparation. Archives of Environmental Health (Chicago) l2(6) :
   705-724, June 1986.

(9) AWES, S. M. Personal Communication. New York, St. Vincent's Hospital
  and Medical Center. [Unpublished] June 1967.
(10) AYBES, 5. M., GIAIPR~LLI, S., JB., Ax~srnona, R. G. Carboxyhemoglobin :
   hemodynamic and respiratory responses to small concentrations. Science
   (Washington) 149 : 193-194, July 9,lQtX.
(11) B~BOEBOX, L. M., JR, EHMICE, D., GOXLUBOL, F., Cae TELLANOS, F. A.,
   S~EQEZL, A., BIXQ, R. J. Effect of cigarette smoking on coronary blood
   flow and myocardial metabolism. Circulation ; Journal of the American
   Heart Association (New York) 15: 251-257, 1957.
(12) Bum&w, S., WEBT, J. W., Mth.nas, 0. F., M~uzorz, U. C. Effect of nicotine
   on the coronary blood flow and related circulatory parameters. Correla-
   tive study in normal dogs and dogs with coronary insutbciency. Circula-
   tion Research 10(l) : 27-34, January 1062.
(23) BENSON, H., COSTAS, R., JB., GAEC~~-PALMIUU, M. R., FEL~~!ZI~TI, hf.,
   Arx~& R., BLAXTO~, J. H., Conon, A. A. Coronary heart disease risk
   factors: A comparison of two Puerto Rican populations. American
   Journal of Public Health and the Nation's Health (New York)
   56(7): 1057-1060, July 1986.

(14) BFJXT, E. W. R. A Canadian study of smoking and health. Ottawa, Depart-
   ment of National Health and Welfare, 1966. 137 pp.
(15) BIXQ, R. J., Bxnn~sa, A., BLUEMCHE~, G., COHEXV, A., GAIUQH~B, J. P,
   ZALUUCI, E. J. The determination of coronary flow equivalent with co-
   incidence counting technic. Circulation ; Journal of the American Heart
   aSsociation (New York) 2Q:S33-840, June 1984.
(18) BIRO, R. J., COHEB, A., BLUEYCH~, G. Tobacco alkaloids and circulation.
   In: Tobacco Alkaloids and Related Compounds. Proceedings of the 4th
   International Symposium held at the Wenner-Gren Center, Stockholm,
   February 1984: 1965. Pp. 241-251.

(17) BOBHANI, N. 0.. HECHTEB, H. H., Basarow, R. Report of a lo-year fol-
   lowup study of the San Francisco longshoremen. Mortality from coronary
   heart disease and from all causes. Journal of Chronic Diseases (St.
   Louis) 16: 1251-3.266, 1963.

(18) BBACHFIZ~, N., Boxxu, J., GOBLIN, R. Action of nitroglycerin on coronary
   circulation in normal and in mild cardiac subjects. circulation ; Journal
   of the &nerican Heart Association (New York) 19: 6Q7,1Q5Q.

70


(19) cED=op: % l!Vmu!m, L., Jouasox, E., KAIJ, L. Morbidity among mono-
   zygot,ic twins. Archives of Environmental Health (Chicago) . lO(2) :
   348350, February 1965.

(20) CEDEBUIF, R. Fmsmto, L, JONSSON, E., KAIJ, L. Respiratory symptoms
   and %ngina pectoris" in twins with reference to smoking habits. Ar-
   chives of Euvironmental Health (Chicago) 13(6) : 726-737, December
    1066.

(31) CuxvbLZEB, R. B., K~UYHOLZ, R. A., Ross, J. c. Reaction of nonsmokers
   to carbon monoxide inhalation. Cardiopulmonary responses at rest and
   during exercise. Journal of the American Medical Association (Chicago)
   X38( 10) : 1061-1064, Dec. 5,1866.

(32) CHUNO, T. S., Lx~~)xas, F. E. Cardiostimulatory responses to vagal stfmu-
   lation, nicotine, and tyramine, American Journal of Physiology (Wash-
   ington) 211(6) : 1443-1446, December 1966.
(23) COHEN, B. H., THOUS, C. B. Comparison of smokers and nonsmokers. II.
   The distribution of Al30 and RH (D) blood groups. Bulletin of the
   Johns Hopkins Hospital (Baltimore) 110: 1-7, January 1962.
(94 ) DA~BIIZ, T. R, KA~~EL, W. B., Rxvors~u~, N., STOKES, J., KAQAN, A.,
   Gosnorv, T. Some factore associated with the development of coronary
   heart disease. Six years' followup experience in the Framiugham study.
   American Journal of Public Health and the Nation's Health (New
   York) 49(10) : 1349-1356, October 1959.
r&55) DOLL, R., HILL, A. B. Mortality in relation to smoking: 10 years' obserra-
   tions of British doctors (pt. 1). British Medical Journal (London)
   l(5395) : 1302-1410, May 30,1964.

(26) DOLL, R., HILL, A. B. Mortality in relation to smoking: 10 years' observa-
   tions of British doctors (Concluded). British Medical Journal (London)
   l(5326) : l-1467, June 61964.

(27) DOLL, R., HILL, A. B. Mortality of British doctors in relation to smoking:
   Observations on coronary thrombosis. In: Haenssel. TV., Editor, Epi-
   demiologlcal Approaches to the Study of Cancer and Other Chronic Dis-
   eases. Bethesda, U.S. Public Health Service, National Cancer Institute
   Monograph No. 19, January 1966. Pp. 205-268.
(28) DOYLE, J. T. Etiology of coronary disease: Risk factors influencing cor-
   onary disease. Modern Concepts of Cardiovascular Disease (Baltimore)
   35: 81-86, April 1966.

(28) DOYLE, J. T., DAWBEE, T. R., KANNJZL, W. B., HESLIN, A. S., KAHN, H. A
   Cigarette smoking and coronary heart disease. Combined experience of
   the Albany and Framingham studies. New England Journal of Medicine
   (Boston) 266(16) : 796-801, Apr. 19,1962.

(30) DOYLE, J. T., ,DAWBEI%, T. IX., KANNF%, W. B., ICINCH, S. H., KAHN, H. A.
   The relationship of cigarette emoking to coronary ho& disease. The
   Second report of the combined experience of the Albany, N.Y., and
   Framingham, Mass., studies. Journal of the American Medical Associa-
   tion (Chicago) lQO(10) : 886-890, Dec. 7, 1964.
(31) DOYLE, J. T., HEBUN, & S., H-ox, H. E., Foaaax~, P. F. Early diagnosis
   of ischemic heart disease. The New England Journal of Medicine (Bos-
   atOn ) 261(22) : 1096-1101, November 1959.

(8.3) Exoxrmvao, H. Cigarette smoking and the in &n, thrombosis of human
   blood. Journal of the American Medical Association (Chicago) 193(12) :
   1033-1035, Sept. Xl,1965
(3.9) ENGELBER~, H., FUTTBEMAN, M. Smoking and acceleration of the throm-
   botic coagulation of blood (Abstract). Circulation ; Journal of the

271304 o-&7---8                          71


American Heart Association (New York) 33-34 (Supplement 3) : O&37,
October 1066.

(54) ENQELBEBQ, H., FUTTEBLIAN, M. Cigarette smoking and thrombotic coago.
   lation of human blood. Archives of Environmental Health (Chicago)
   14 : 266-270, Foblmary lD67.

(3.5) tiEWEIR, F. H. The epidemiology of coronary heart disease : A review.
   Journal of Chronic Diseases (St. Louis) 18(8) : 735-774, August lQ65.
(36) &!JTI!!IN, F. H. Some uses of prospective observations in the Tecumseh
   Community Health Study. Proceedings of the Royal Society of Medi-
   tine (London) 60: S&60, January 1967.
(37) EYBENCK, H. J., T-w, M., WOOLF, M., E~e~aru~, L. Smoking and per-
   sonality. British Medical Journal (London) l(6I.84) : 1456-1460, May 14,
    1960.

(%j FOLLE, L. E., S~ANEK, Y., Avra~o, D. M. Cardiopulmonary effects of to
   baoco and related substances. II. Coronary vascular effects of cigarette
   smoke and nicotine. Archives of Environmental Health (Chicago)
   E?(6) : 7l2-716, June 1986.

(39) Fox, S. M., III, SICIRXEB, J. 8. Physical activity and cardiovascular
   health. American Journal of Cardiology (New York) 14(6) : 731-746,
    December 1964.

(40) FBANX, C, W., WEINBLAT~, E., SHAP~O, 5.. SAQJEB, R. V. Myocardial in-
   farcation in men. Role of physical activity and smoking in incidence
   and mortality. Journal of the American Medical Association (Chicago)
   198(12) : .l241-l245, Dec. 19, 1266.

(41) FBANK, C. W., WEL~BLATT, E., SHAP~BO, S., SAQBB, R. V. Physical inac-
   tivity as a lethal factor in myocardial infarction among men. Circula-
   tion ; Journal of the American Heart Association (New York) 34: 1022-
   1033, December 1366.

(&) FEIEDMAN, G. D. Cigarette smoking and geographic variation in coronary
   heart disease mortality in the United States. Presented at Conference
   on Epidemiology of Cardiovascular Diseases, American Heart Associa-
   tion, Chicago, Illinois, Feb. 5, 1967. [Unpublished.] 23 pp.
(&?) GLYNN, M. F., MUST~BD, J. F., BUCHANAN, M. R., MUBPHY, E. A. Cigarette
   smoking and platelet aggregation. Canadian Medical Association Jour-
   nal (Toronto) 95 : 549-653, September 1366.
(64) GOBLIN, R., BBACEFELLI, N., MACLEOD, C., BOPP, P. Action of nitroglycerin
   on coronary circulation in normal and in mild cardiac subjects. Circu-
   la&ion ; Journal of the American Heart Association (New York) 19 : 705,
    1959.

(4.5) GEEQQ, D. E., FISHES, L. C., Editors. Blood supply to the heart. In: Dow,
   P., editor. Handbook of physiology. Circulation. See. 2. Washington,
   American Physiology Society, 1963. Pp. 1563.
(46) HAEN~ZEL, W., SHIYEIN, M. B., MILLEB, H. P. Tobacco smoking patterns
   in the United States. Washington, U.S. Department of Health, Educa-
   tion, and Welfare, Public Health monograph No. 45. Public Health Serv-
   ice publication No. 463,1956.111 pp.

(47) HA~IMO~D, E. C. Smoking in relation to the death rates of 1 million men
   and women. In: Haenszel, W., editor. Epidemiological approaches to the
   study of cancer and other diseases. Bethesda, U.S. Public Health SerV-
   ice, National Cancer Institute Monograph No. 19, January 1966. Pp.
    l27-204.

(48) HEATH, C. W. Differences between smokers and nonsmokers. American
   Medical Asociation Archives of Internal Medicine (Chicago) IO1 : 377-
   388, February 1958.

72


(49) H.IQQINs, M., K~snsmmo, M., Msrsm H. Characteristics of smokers and
   nonsmokers in Tecumseh, Mich. I. The distribution of amoking habits'in
   persons and families and their relationship to soda1 characteristics (In
   press). American Journal of Epidemiology (Baltimore) : 1997.
(50) HIQQINS, M., KJELSBEBGI, M. Characteristics of smokers and nonsmokers
   in Tecumseh, Mich. II. The distribution of selected physical measure-
   ments and physiological variables and the prevalence of certain diseases
   in smokers and nonsmokera (In press. j American Journal of Epidemi-
   ology (Baltimore) : 1997.27 pp.
(51) JENX~XS, C. D. Personal communication. The University of North Caro-
   lina, Chapel Hill, Apr. 16, 1967.
(5,9) KAHN, H. A. The Darn study of smoking and mortality among U.S.
   veterans: report on 8% years of observation. In: Haenssel, W., editor.
   Epidemiological approaches to the study of cancer and other diseases.
   Bethesda, U.S. Public Health Service, National Cancer Institute Mono-
   graph No. 19, January 1969. Pp. l-125.
(53) KANNEL, W. B. Cigarette smoking and coronary heart disease (editorial).
   Annals of Internal Medicine (Philadelphia) 90(6) : X103-lIO9, Jane
    1994.
(54) KANNEL, W. B., D~wrtss, T. R., COHEN, I& E., MCNAMARA, P. hf. Vascular
   disease of the brain-epidemiologic aspects: The Framingham study.
   American Journal of Public Health and the Nation's Health (New
   York) 55(Q) : 13664399, September 1986.
(55) KAX~EL., W. B., DAWBQZ, T. R., M~NAMABA, P. M. Detection of the coro-
   nary-prone adult : The Framingham study. Journal of the Iowa Medical
   Society (Des Moines) t%(l) : 26-34, January 1999.
(56) KEIIEA, M., KOBOLICO, A. Tobacco smoking and blood clotting. Bulletin of
   Polish Medical Science and History (Chicago) 8: 14!%148, October
    1995.
(573 KESSHB~UX, A., Bmr.trr, S. Cigarette smoking and blood lipids. Journal
   of the American Medical Association (Chicago) 187(l) : 32-33, Jan. 4,
    1994.
(58) KE;~SH~AUM, A., Bm, S. Smoking as a factor in atherosclerosis.
   Geriatrics (Minneapolis) 2l(l2) : XX-170, December 1999.
(59) KE~SHBAUM, A., BELLET, S., JIMENE~, J., Fs~nsnso, L. J. Differences in
   effects of cigar and cigarette smoking on free fatty acid. Mobilization
    and catecholamine excretion. Journal of the American Medical Asso-
   ciation (Ohicago) 195(13) : 1995-1999, Mar. 28,1998.
(60) KRUMHOLZ, R. A. Pulmonary membrane dilfusing capacity and pul-
   monary capillary blood volume: An appraisal of their clinical useful-
   ness. American Review of Respiratory Diseases (Baltimore) 94( 2) :
   196299, August lQtX3.
(61) K~UMHOL~, R. A., CHEVALZ~~, R. B., Ross, J. C. Cardiopuhnonary func-
   tion in young smokers. A comparison of pulmonary function measure-
   ments and some cardiopulmonary responses to exercise between a
   group of young smokers and a comparable group of nonsmokers. Annals
   of Internal Medicine (Philadelphia) @I(4) : 903-610, April 1994.
(62) KRUMHOLZ, R. A., UHEV~, R. B., Ross, J. C. Changes in cardiopul-
   monary functiona related to abstinence from smoking. Studies in young
   cigarette smokers at rest and exercise at 3 and 6 weeks of abstinence.
   Annals of Internal Medicine (Philadelphia) 62(2) : 197-207, February
    1996.


(69) LABZON, R. II., FUKUDA, P., MU~BAY, J. F. Systemic and pulmonary vas.
   cnlar effects of nicotine in anesthetized dogs. American Review of Res.
   piratory Diseases (Baltimore) Ql(4) : 556-564. April 1965.
(64) LEADING, F. E., Loxo, J. P. Action of nicotine on coronary vascular iv+
   slstance in dogs. American Jotmral of Physiology (Washington) 2CJ3(4) :
   621-625, October lm

(65) LZLIJZIYFELD, A. M. Emotional and other *elected characteristics of ciga,
   rette smokers and nonsmokers as related to epidemiological studies of
   lung cancer and other diseases. Journal of the National Cancer Institute
   (Washington) 22(2) : 25Q-@2, lQ5Q.

(66) Lrsnzw~oz, J. Badania Doswiadczalne Wplywu Palenia Popierosow na
   Czas Krzepniecia Krwi i Osocza Uwapnionego Ora Czas Protrombinowy
   i Trombbowy Ozocza u Ludzi. Polski Tygodnik Lekarski (Warszawa)
   18: 147x-1473, lQ83.

(67) LUZVDMMT, T. Smoking in relation to coronary heart disease and lung
   function in twins. A cotwin control study. Acta Medica Scandinavica
   (Stockholm) 180 (Supplement 455) : l-75, lQ83.
(68) MOABTHUB, C., WAL~BO~, E., DI~KI~SON~ J. The .psychology of smoking.
   Journal of Abnormal Psychology (Washington) 58: 287-275, lQ58.
(69) MATABUZO, J. D., SASMW, (x. Pzychologlcal and related characteristics
   of smokers and nonsmokers. Psychological Bulletin (Washington)
   5'7(6) : 483513, November 1980.

(70) Mu~~arso~, L. E., Frrq T. Effects of cigarette smoking on serum-lipids,
   Iblood-glucose, and platelet adhesiveness. Lancet (London) 2: 132-134,
   July lM3.

(71) MUBPHY, E. A., Personal communication. University of Colorado, Febru-
   ary 1967.

(7.9) MOBPEY, E. A., MUSTARD, J. F. Tobacco and thrombosis. American Journal
   of Public Health and the Nation's Health (New York) 56(7) :
   lQ61-lQ73, July 1986.

(76) MUSTARD, J. F., Muara~, E. A. Effect of smoking on blood coagulation
   and platelet survival in man. British Medical Journal (London)
   l(5334) : 846434Q, Mar. 30, 1983.

(74) P~MBOEE, R. S., JR. WIUUXS, J. L. Youthful precursors of fatal
   stroke. Circulation: Journal of the American Heart Association (New
   York) 33-34 (Supplement 3) : 183-184, October 1968.
(75) P~~zz, R. S., Ja., WINQ, A. L. Characteristics in youth predispos-
   ing to fatal stroke in later years. Lancet (London) 1: 753-754, Apr. 3,
    1967.

(76) P-mini, R. S., JB., WOLP, P. A., Norzxz, J., Taoanz, Id. C. (4hronic
   disease in former college etudents. I. Early precursors of fatal coronary
   heart disease. American Journal of Epidemiology (Baltimore) 83(2) :
   314?32&1968.

(77) PA~NELL, R. W. Smoking and cancer. Lancet (London) 1: Q33,lQ51.
(78) PAUL, 0.. LEPPEB, M. H., PH~LAN, W. H., DUPEBITITJS, G. W., MAoMILLaz,
   A., MoUa, H., PABE, H. A longitudinal study of coronary heart
   disease. Circulation; Journal of the American Heart Association (New
   York) 23: 20-31, July 1983.

(79) REQAN, T. J., FRANK, M. J., MoGri-wr, J. F., Z~BL, E., HELLEMS, H. K.,
   BINQ, R. J. Myocardial response to cigarette smoking in normal subjects
   and patients with coronary disease. Clmlation ; Journal of the Amer-
   ican Heart Association (New York) X$3(3) : 335-3@, March 1961.

74


(89) RrusLi!Z& 8. H., TBA~~LL, J., KaBp, D. Detection of coronary athero!xle-
   rosis in the living animal by the ergonovine stress test Science (Wash-
   ington) lZi: Soo, 1968.
(81) ROSEIWAN, B. H., FIUEDXAX?, M., JENKINS, D., Sra~us, R., Wun& M.,
   KOSITCEEK, It. The prediction of immunity to coronary heart disease.
   Journal of the American Medical Association (Chicago) lQ&(ll) : 115Q-
   1162, Dec. X&1966.
(89) ROSENMAN, R. H., FB~EDMAN, M., STBAUS, R, WWY, M., KOSITCHEK, R.,
   HAHN, W., WE~TEES~EN, N. T. A predictive study of coronary heart
   disease. Journal of the American Medical Association (Chicago) 189(l) :
   15-22, July 61964. [Data in Table 13, this chapter, Unpublished.]
(89) RUSSEK, H. I. Stress, tobacco, and coronary disease in North American
   professional groups. Survey of l2,ooO men in 14 occupational groups.
   Journal of the American Medical Association (Chicago) lQ2(3) : 13Q-
   194, April IQ, 1965.
(84) SAIBEB, E. J., MACMAHON, B. Cigarette smoking among high school stu-
   dents related to social class and parental smoking habits. American
   Journal of Public Health and the Nation's Health (New York) 51(12) :
   1780-1789, December 1961.
(85) SAMANEK, M., A-0, D. M. Cardiopulmonary effects of tobacco and re-
   lated substances. III. Pulmonary vascular effects of cigarette smoke and
   nicotine. Archives of Environmental Health (Chicago) l2(6) : 7l7-724,
    June 1966.
(86) SCHOB, S. 9.. ELSOM, K. A, ELSOX, K. O., DUNN, J. P. An evaluation of
   the periodic health examination: A study of factors discriminating
   between survival and death from coronary heart disease. Annals of
   Internal Medicine (Philadelphia) 61(6) : 1666-1014, December 1964.
(87) SWANI, R. K., JOSHI, K. C. Effect of cigarette and biri smoking and
   tobacco chewing on blood coagulation and flbrinolytic activity. Indian
   Heart Journal (Calcutta) 17: 233-242, July 1966.
(88) SPAIN, D. M., NATHAN, D. J. Smoking habits and coronary atherosclerotic
   heart disease. Journal of the American Medical Association (Chicago)
    177 (10) : 633633, September 1961.
(89) STBONQ, J. P., MCGILL, H. C., JR, RICHILBDS, M. L., Eeouu, D. A. Relation-
   ship between cigarette smoking habits and coronary atherosclerosis in
   autopsied males. Circulation: Journal of the American Heart Associ-
   ation (New York) 33-34 (Supplement 3) : 31, October 1966.
(90) SYJKE, S. L., BOBIUNI, N. O., BTJECHLEY, R. W. Cultural mobility and
    coronary heart disease in an urban area. American Journal of Epi-
   demiology (Baltimore) 83(3) : 334346, November 1968.
(91) SYME, 8. L., H~AN, M. M., ENTEBLINE, P. E. Some social and cultural
    factors associated with the occurrence of coronary heart disease. Jour-
   nal of Chronic Diseases (St. Louis) 17: 277-289, 1964.
(99) SYYE, S. L., Rnumm, L. G., editors. Social stress and cardiovascular dis-
   ease. Proceedings of the National Workshop Conference on Socio-environ-
    mental Stress and Cardiovascular Disease, Phoenix, Arm., Feb. 14 to 16,
   1968. The Milbank Memorial Fund Quarterly (New York) 45 (No. 2,
   pt. 2 ) : l-192, April 1987.
(99) TuoxAs, C. B. Characteristics of smokers compared with nonsmokers in a
   poPnlatiOn of healthy young adults, including observations on family
   history, blood pressure, heart rate, body weight, cholesterol, and certain
   psychologic traits, Aunals of Internal Medicine (Philadelphia) 53 :
   6Q7-718, October 1980.


(94) TMVEU, J., RINZUCB, 8. H., 9hBp, D. Cardiac effects of nicotine in the
   rabbit with experimental coronary atherosclerosis. Annals of the New
   York Academy of Sciences 90 : 290301, Sept. 27, 1960.
(95) TBUETT, J., COB-, J., K~anx~, W. A mnltivariate analysis of the risk
   of coronary heart disease in Framingham. ClJnpublished.1 26 pp.
(96) U.S. Pumrc HEALTH Sxavrcx. The Framingham Heart Study. Habits and
   Coronary Heart Disease. Washington, U.S. Department of Health, Edu-
   cation, and Welfare, Public Health Service, Publication NO. 1515, 1968.
(97) U.S. PWLIC HEALTH SEB~XE. National Center for Health Statistics. Mor-
   tality from diseases associated with smoking: United States, 1980-84.
   Washington, U.S. Department of Health, Education, and Welfare, Vital
   and Health Statistics Series 20, No. 4, Public Health Service Pnblica-
   tion No. XNXJ, October 1988.45 pp.

(98) U.S. PDBLI~ HEALTH Sxavrcn. National Center for Health Statistics. Mor-
   tality trends in the United States: 1954-03. Washington, U.S. Depart-
   ment of Health, Education, and Welf'are, Vital and Health Statistics
   Series 20, No. 2, Public Health Service Publication No. lQQ0, June 1QQQ.
    57 PP.

(99) U.S. PWLIC HEALTH SEBVICE Smoking and Health. Report of the Advisory
   Committee to the Surgeon General of the Public Health Service. [Wash-
   ington.] U.S. Department of Health, Education, and Welfare, 1964.
   337 PP.

(100) WEINBLATT, E. Personal communication. Health Insurance Plan of Greater
    New York, Apr. 17,1987.
(201) WEBLJC, E., Scrrrxvmnmx, H. Aotivity of nicotine and inactivity of kal-
   likrein and kallidin in aggregation of ablood platelets. Nature (London)
    207 : 871-872, Aug. 21,1985..

(199) WESTFALL, T. C., CIPOLLO~VI, P. B., E~m~mom~cz, A. C. Influence of pro-
    pranolol on hemodynamic changes and plasma catecholamine levels
    following cigarette smoking and nicotine. Proceedings of the Society
    for Experimental Biology and Medicine (New York) 123 : 174-17Q,lQ66.

SUPPLEIMENTAL REFERENCES

Sl. ABMITAGE, A. K. Ei7ects of nicotine and tobacco smoke on blood pressure
  and release of catechol amines from the adrenal glands. British Journal
  of Pharmacology and Chemotherapy (London) 25 : 515-526, October
   1985.

52. BALO~, J. A~rxa, >I., REBCH, J. A. Cerebral thromboembolism. A clinical
  appraisal of 100 cases. Neurology (Minneapolis) 16 : 559-534, June 1984.
S3. BELLET, 5. Atria1 Abrlllation in the elderly patient. Geriatrics (Minne
  apolis) 21(11) : 234-245, November 1968.
S4. BEET, E. W. R., WALKEFL, C. B., BAKEB, P. M. DELAQUIS, F. M., NCGBEQO~
  J. T., &Kxxzrx, A C. Summary of a Canadian study of smoking and
  health. Presented at the Annual Meeting of the Canadian Public Health
   Association, Quebec, June l,lQ66.7 pp.

55. BESTEBMAN, E., MYAT, G., !J?BAVALU, V. Diurnal variations of platelet sticki-
  ness compared with effects produced by adrenaline. British Medical
   Journal (London) 1: 597-600, Mar. 11.1967.
56. BINQ, R. J. The effect of nicotine and vasopressin on capillary capacity and
   capillary blood flow. [Unpublished.] 7 pp.


S7. BISCAEDI, A. M., WASSEBMANN, G. F. Release of catecholamines from
  adrenalinogenic and noradrenalinogenic tissue by the action of nicotine
   9% vitro." Archives Intemationales de Pharmacodynamie et de Therapie
  (Gand) 159(2) : 424-433,1966.

38. BLACKBUIM, H. PM, R. W., Kxxs, A. The inter-relations of electrocardi-
   ographic llndings and physical characteristics of middle-aged men. Acta
   Medics Scandinavica Supplementum (Stockholm) 466 : 316-341, 1967.
~9. BWXBWN, H., TA~OB, H. L., PABLIN, 3. W., KIHLBEZBQ, J., IGZYS, A.
   Physical activity of occupation and cigarette smoking. Relationship to
   ventilatory function and respiratory symptoms. Archives of Environ-
   mental Health (Chicago) lO(2) : 312322, February 1965.
~10. BOBHANI, N. O., HECHTE~, H. H. A longitudinal study of blood pressure.
   Angiology (New York) 15(I2) : 545-555, December 1964.
~11. BBAKMAN, P., ALBB~CHTSEIP, 0. K., ASTBU~, T. Blood coagulation, flbrinol-
  ysis, and contraceptive hormones. Journal of the American Medical
   Association (Chicago) 199 (2) : 89-74, Jan. Q,lQ67.
SE. BBESLOW, L., BUELL, P. Mortality from coronary heart disease and phys-
   ical activity of work in California. Journal of Chronic Diseases (St.
   Louis) ll(4) : 421-444, April lD60.

~13. BBOWR, R. C., RITZMANN, L. Some factors associated with absence of cor-
   onary heart disease in persons aged 65 or older. Journal of the American
   Geriatrics Society (Baltimore) 16 (3) : 23Q-250,196'7.
s14. BUBCH, P. R. J., ROW~LL, N. R. Smoking and atherosclerosis (letter).
   British Medical Journal (London) 1: 1050-1051, Apr. 23.1988.
Sl5. Bum, J. II., GIBBONS, W. It. The release of nor-adrenaline from eympathetic
   fibres in relation to calcium concentration. Journal of Physiology (Lon-
   don) 181: 214223, November 1965.

S16. BUTKUS, A., PAQE, I. H. Smoking and postabsorptive serum lipid levels.
   Journal of the American Medical Association (Chicago) 192(l) : 52-53,
   Apr. 5,1D65.

Sl?. Can carbon monoxide raise blood cholesterol levels? Medical World News
   (New York) 8(l) : 26-27, Jan. 6,1D67.

S18. CED~XL~F, R. Urban factor and prevalence of respiratory symptoms and
   "angina pectoris." A study of 9,168 twin pairs with the aid of mailed ques-
   tionnaires. Archives of Environmental Health (Chicago) 13(6) : 743-
   748, December 1966.

519. CEXIEBLOF, R., JONSSON, E., LUNDMAN, T. On the validity of mailed ques-
   tionnaires in diagnosing "angina pectoris" and "bronchitis." Archives
   of Environmental Health (Chicago) 13(6) : 738-742, December 1968.
S20. Cigarette smoking and heart disease. British Medical Journal (London)
   2 : 1404-1405, Dec. 10,1966.

821. COHEN, A., Lws, E., Z&ESKI, E. J., BINS, R. J. A new diagnostic test
   for coronary artery disease using a coincidence counting system. Minne-
   sota Medicine (,St. Paul) 49 : 17-21, January 1986.
822. COHEN, A., 51xsx1, E. J., LUEBS, E., BIN& R. J. The use of positron emit-
   ter in the determination of coronary blood flow in man. Journal of Nu-
   clear Medicine (Chicago) 6 : 651-866.1965.
F&S. COHEN, B. H., THOMAS, C. B. Comparison of smokers and nonsmokers. II.
   The distribution of ABO and Rh (D) blood groups. Bulletin of the Johns
   Eo~kins Hospital (Baltimore) 110(l) : l-7, January 1962.
824. C~AMEB, K., PAULIN, S., WEBKO, L. Coronary angiographic findings in cor-
   relation with age, body weight, blood pressure, serum lipids, and smok-
   ing habits. Circulation ; Journal of the American Heart Association (New
   York) 33 : 888-900, June 1968.

77


825. CBETJX, G., HABTMANN, G., WIDMEB, L. K., GBEENSHEB, A., Bm% H., BEBEB,
   H., STAUB, H. KAUFMANN, L. Zur Be&hung Zwischen Llpiden, Blutdrnk
   und Gewicht bei Rauchem-Basler Studie. Verhundlungen der Deu&hen
   Gesellschaft fur Kreislanfforschung (Darmstadt) 28 : 25Q-262, 1983.
S26. CBOFTON, E., CBOFTON, J. Influence of smoking on mortality from various
   diseases in Scotland and ti England and Wales. An analysis by cohorts.
   British Medical Journal (London) 2 : 1161-1164, November 9, lQ63.
S27. CBOSEITI, L., Rururvo, G. F., PETTINATI, L. Osservasioni in tema di
   detossicasione dell' emoglobina nell'nomo esp0et.o a rischio prolungats
    da ossido di carbonio. Mlnerva Medica (Torino) 67: 268-26Q, 1966.
528. CUBPHEY, T. J., HOOD, IA P. L., ~BKINS, N. M. Carboxhemoglobin in rela.
   tion to air pollution and smoking. Archives of Environmental Health
   (Chicago) 10 : 179-185, February lQ65.

529. DABLSTB~M, H., W~BDSTIUX-~)HBBEW, U., ROTHSCHILD, A. The influence of
   tobacco smoking, and increased initial carbon monoxide concentration on
   results of Sj6strand's method of total hemoglobin determination. Acta
   Physioligica Scandinavica (Stockholm) 42 : 174-184,1958.
530. D~~sxa, T. R., KANX~L, W. B., L~ELL, L P. An approach to longitudinal
   studies in a community : The Framingham study. Annals of the New York
   Academy of Sciences 107 : 53Q-556, May 22.1983.
S31. DAWBE~, T. R., KANN~L, W. B., McNA~~AE& P. M. The Prediction of core.
   nary heart disease. Transactions of the Association of Life Insurance
    Medical Directors of America (New York) 47 : 70-105,1Q64.
532. DAWBEB, T. R., KANNEL, W. B., Rxvorsx~~, N., KAQAN, A. The epiden&
   ology of coronary heart disease-the Framingham enquiry. Proceedings
   vf the Royal Society of Medlclne (London) 56: 265-271, April 1982.
933. D~sormx, H., TBDFFEEGT, H., Lxssx, J., PABEBT. Sur l'oxycarbomknie des
    fumeurs. Archives des Maladies Professionneles, de Mededne da
   Travail et de Securite Sociale (Paris) 23(Q) : 5'19682, September 1982.
534. DOCK, W. Ballistocardiographic patterns and nicotine. Types of ball&o.
   cardiographic patterns evoked by nicotine and the relation of the most
    frequent pattern to effects of nicotine on respiration, not on the heart.
   Archives of Internal Medicine (Chicago) 1l2 (4) : 467-475, October 1963.
935. DOBM~Y, T. L. Abnormal oxygen-dissociation curves. (letter), Lancet
    (London ) 2 : 80-81. July lO,lQ65.

536. DOYLE, J. T. Tobacco and the circulation apparatus. National Conference
   on Cardiovascular Diseases (Washington) 2 : 361-362,X)64.
937. DUNN, J. E., JB., LINDEN, G., Bmsmw, L. Lung cancer mortality experi-
   ence of men in certain occupations in Callfomia. American Journal of
   Public Health and the Nation's Health (New York) 56 (10) : 1475-1487,
    October 1980.
S38. EHBLICH, S. P.,  JR Seal Beach Stroke Study. November 1966.
    [Unpublished.] 8 pp.

539. EISEN, hi. E. Coexistence of thromboangiitis obliterans and arterio-
   sclerosis: Relaationship to smoking. Journal of the American Geriatrics
    Society (Baltimore) 14 (8) : 846-858.1966.

S40. ELIOT, R. S., MIZUKA~~I, H. Oxygen a&&y of hemoglobin in persons with
   acute myocardial infarotion and in smokers. Circulation; Journal of
   the American Heart Association (New York) 34 : 331-336, August 1966.
541. EPSTEIN, F, H., OBTBANDEB, L. D., Ja, JOHNSON, B. C., PAYNE, Y. W.,
   HAYNEB, N. S., KELLEB, J. B., FB~NCIS, T., JB. Epidemiologlcal studies
   of cardiovascular disease in a total community.-Tecumseh, Mich.
   Annals of Internal Medicine (Philadelphia) 62 (6) : 1170-1187, June 1986.

78


542. E~PINEB, E. A., TUCCI, J. EL., JAGQEB. P. I.. L~tuxn. D. P. Independence of
  blood pressure and aldosterone secretion. New England Journal of
   NedMne (Boston) 276114) : 734-733, ADR 16.1967.
g43, FrsnzR, C. M., Gozz, I., OKABE, N., WHITE, P. D. Atherosclerosis of the
   car&id and vertebral arteriee-extracranial and intracranial. Journal
  of Neuropathology and Experimental Neurology (Baltimore) 24:
   455-4'76. July 1965.

SQ. INK, C. W., WEINBIATT, E., SEAPIXI, S., Smmw, G. E., SAQE& IX. V. The
   H.I.P. study of incidence and prognosis of coronary heart disease:
   criteria for diagnosis. Journal of Chronic Diseases (St. LOUIS) 16 :
   1293-1312, December 1963.

~43. Fuxx~, W. S., FEEDMAN, R., SOLOFF, L. A. Cardiac output, blood pressure,
   and free fatty acid responses to smoking in the nonbasal &ate. American
   Journal of the Medical Sciences (Philadelphia) 252 : 39-44, July 1966.
~46. FBANKL, W. S., WINTJQZB, W. L., SOLOFF, L. A. The effects of smoking on
   the cardiac output at rest and during exercise in patients with healed
   myocardial infarction. Circulation ; Journal of the American Heart
   Association (New York) 31(l) : 42-44, January 1965.
~47. GABBISON, G. E., MCDONOU~H, J. R., HANSEL, C. G., STULB, S. C. Prevalence
   of chronic congestive heart failure in the population of Evans County,
   Ga. American Journal of Epidemiology (Baltimore) 33(2) : 333-344,
    1966.

~48. GEBBEB, G. L., VOLLE, R. L. Mechanisms involved in ganglionic blockade
   induced by tetramethylammonium. Journal of Pharmacology and Ex-
   perimental Therapeutics (Baltimore) 152(l) : 13-23, April 1966.
S-19. GEBTLEB, M. N., WHIT% H. H., WELBE, J. J. Assessing the coronary pro-
   flle. Geriatrics (Minneapolis) 22(2) : 121-132, February 1967.
SBO. GIBELLI, A., BOLAND~~A, E., DEL FAVEBO, A., PAS@~TI, C. Modificazioni
   emocoagulative e della llbrinolisi in seguito a carico lipidic0 orale
   associate a fumo da sigaretta. Giornale di Gerontologia (Firenze) 12:
   314.1964.

S51. GITTEL~JOHN, A., HU~CE, S. Applications of life tableanalysis and automatic
   computer selection of population subgroupings for cardiovascular epi-
   demiologic studies. Annals of the New York Academy of Sciences 126:
   767-778, 1965.

852. Hair, J. I., LAO, 5. II., DAMATO, A. N. Acute effects of smoking in the dog.
   Clinical Research (New York) 14 (4) : 472, December 1866.
853. HAMMOND, E. C. The effects of smoking. Scientific American (New York)
   207 (1) : 3-15, July 1962.

%a. HAMMOND, E. C. Smoking in relation to mortality and morbidity. Fin&ngs
   in first 34 months of follomup in prospective study started in 1959.
   Journal of the National Cancer Institute (Washington) 32 (5) : 1161-
   1133, Nay 1964.

S55. HAzuvv, J., PEELMAN, E. Tobacco allergy in coronary artery disease. New
   York State Journal of Medicine (New York) 64 : 1287-1296, June 1.1964.
~6. HABuN, W. R., JB., OBEBMAN, A., Mrroaz~~, R. E., Gaarmz~, A. Consti-
   tutional and environmental factors related to serum lipid and lips-
   Protein levels. Annals of Internal Medicine (Philadelphia) f36(3) :
   540-555. March 1967.

557. mss, G. M., bnEBHOLM, W., HEMMENB, A. Production of calchlc
   atheroarteriosclerosis and thromboarteritis with nicotine, vitamin D and
   d1etar-y cholesterol. american Journal of Pathology (New York) 49(4) :
   739-741, October 1966.

79


558. HJZINE, H., SCHMIDT, H., ANDEB~, G. Klinisches Bild und Therapieresultate
   bei Patienten mit Endangitis obliterans und peripherer Arterioeklerose.
    Angiologlca (Base1 ) 2 : 195-2II. 1965.

S59. HEPDEH-STUQ~Y, S. Epidemiologic des Herzinfarktes (1965). Schwh
   rische ~Medizinische Wochenschrift (Basel) fJB(45) : 1535-1546, Novem
   ber 6, ,liM35.

560. HIN-, L. E., J& The use of a large industrial population to study the
   effects of social and behavioral factors on coronary heart disease. Amer.
   ican Journal of Public Health and the Nation's Health (New York) 56
    (9) : 1476-1475, September 1986.

561. HI~PKL& L. E., Jk, BEHJAYIX, B., C~rusrzz80~, W. N., Uw N, D. 8.
   Coronary heart disease. myear ezperience of 1,160 men. Arehives of
   Environmental Health (Chicago) 13: 312321, September 1366.
562. HOLLAND, W. W.. R&mm. E. B., MCPFIEB~ON. P., 'STONE, R. W. A eardio
   vascular survey of American East Coast telephone workers. American
   Journal of Epidemiology (Baltimore) 35 (1) : 61-71,1967.
563. JOHANNSEN, Zi., PBEIBISZ, J. Bezposrednl Wplyw Palenia Papierosow na
   Spirogram u Zdrowych i Chorych z Rodzedma Pluc i Przewlesklym
   Niezytem Oskrzeli. Polskie Archiwum WXIYCYIIY Wewnetrznej
    (Warszawa) 36(6) : 733-736.1966.

964. KAQAN, A., KANX~L, W. B., DAWBEB, T. R., REVOTBKCIE, N. The coronary
   profile. Annals of the New York Academy of Sciences 97: 333-394,
    Aug. 29,1963.

9%. KANNEL, W. B., Epidemiology of cerebrovascular disease : An epidemiologic
   study of cerebrovascular disease. In: Cerebral Vascular Diseases. New
    York. Grune & Stratton, 1966. Pp 5356.
S66. KAN~L, W. B., Habits and coronary heart disease. The Framingham
   Heart Study. Bethesda, National Heart Institute. U.S. Public Health
   Service Publication No. i515,1966. Unpaged.
S67. KANNEL, TV. B., KAQAN, A., DA-, T. R., REIWTSKIE, N. Epidemiology of
   coronary heart disease. Implications for ,the practicing physician.
   Geriatrics (Minneapolis) 17 (10) : 675-6fNl, October 1962.
568. KASAI, T., POLLAK, 0. J. Action of cigarette smoke on rabbits' cardio-
   vascular cells in vitro. Tohoku Journal of Experimental Medicine
    ( Sendai) 33 : 245-252, 1964.

SBQ. KASANEN, A., Fosssmo~, J. Eating and smoking habits of patients with
   myocardial infarction. Annales Medecinae Internae :Fenniae (Helsinki)
    55(l) : 7-11, 1966.

570. KEDRA, N., DXOWEKI, G. The influence of tobacco smoking on the develop
   ment of atherosclerosis and on ,the composition of blood lipids. Polish
    Nedical Journal (Warszawa) 5 : 37-43,X%6.
S71. KEDIU, M., KOBOLKO, A. Palenie tytonin a Krs e pniecie Krwi. Polski
    Tygodnik Lekarski (Warszawa) 21: 44-46,1968.
572. KEBSH~AUM, A, BELLET, S., KHOBBAD~AN, R. Elevation of serum cholesterol
   after ad.ministration of nicotine. American Heart Journal (St. Louis)
    60: 266-210. February 1965.

573. Kzzsa~~uaa, A., J~bizrurz, J., BELLET, S., ZANUTTINI, D. Modification of
   nicotine-induced hyperlipidemia by antiadrenergic agents. Journal of
    Atherosclerosis Research (Amsterdam) 6: 524-530.1966.
574. Kz~s, A., BLACK~K~N, H. Background of the patient with coronary heart
   disease. Progress in Cardiovascular Diseases (New York) 6(l) : 14-44
    July 1963.

S75. KIBKEBY, K. Blood lipids, lipoproteins, and proteins in vegetarians. Acb
   Nedica Scandinavica (Stockholm) Supplement No. 443 ; l-34, 1966.

80


~76. KnmHNEl3, N., f&m!& H. J., SMITE, W. J., JCmxri?~8, A.. G. Release of cate-
   cholamines and specillc protein from adrenal glands. Science (Washing-
   tin) 154 : 5294531, Oct. 23,1066.

877. KI.ENSCE, H. Die Reaktivatit des Kreislaufes bei Gesunden und Koronar-
   kranken nach dem Rauchen einer Zigarette. (Neue Ergebnisse mit einem
   quautitativen BKG-Ran&test) . Archiv flir KreislauiIorschuug : Beihefte
   zur Zeitschrift f5r Kreislaufforschung (Darmskadt) 41: l-26, July 1963.
878. KONTTINEN, A. Cigarette smoking and serum lipids in young men. British
   Medical Journal (London) l(5285) : 1115-1116, Apr. 2I, 1962.
sig. KEUE~EB, D. E., W-MB, J. L., PAFF~NB~BOEB, R. S., JB. Trends in death
   rates from cerebrovascular disease in Memphis, Tennessee, 1920-60.
   Journal of Chronic Diseases @t. Louis) 26: I2%137, 1967.
880. KWMN, H. C., Asrzup, T. Aortic arteriosclerosis in rabbits. Archives of
   Pathology (Chicago) 78 : 474-482, November 1934.
~81. LANDEBHOLM, W., Hic&f&f~x~, A., &as, G. M. An experimental inquiry into
   ni&ineinduced thrombarteritis. Federation Proceedings (Baltimore)
   26 (2) : 359, March-April 1967.

~82. LICHTACAN, M. A., WOODS, J. W. Catecholamine escretion in young white
   and negro males with normal and elevated blood pressure. Journal of
   Chronic Diseases (St. Louis) 20 : 11~I281967.
88.3. LIEBEB, I. I., GOLDBTEIN. B., G-o, J. C., STEINMAN, J., B~UNO, E., Hza-
   SZKOWICZ, A., N~~LIN, J. B. Posible atenuaci6n biocatalitica de 10s
   efectos nocivos de .la nicotina en fumadores con afecciones cardiovascu-
   lares. Fundamentaci6n quimica y experimental. Revista oto-neuro-oftal-
  moldgica y de cirugia neuroli3gica sud-americana (Buenos Aires) 41(7/
   12) : 55-56, July 1966.

s8.l. LOBS, E. D., COHEN, A., ZILLEBKI, E., Z~~HLKE, V., BIHO, R. J. Die Bestim-
   mung der Koronardurchflussmege beim Menschen mit dem Isotop Rubid-
   ium-84 und einem Koinzidenzmesssystem und ihre diagnostische Aus-
   mertung. Zeitschrift ffir Kreislaufforsehung (Darmstadt) 54( 12) : 12l7-
   1228,X%35.

S85. Luzss, E., COHEN, A., ZAL~SKI, E. J., BINS, R. J. Effect of nitroglycerin,
   intensain, isoptin and papaverine on coronary blood flow in man meas-
   ured by the coincidence counting technic and rubidium. American Jour-
   nal of Cardiology (New York) 17 : 535-541, April 1966.
St%. LUPU, N. G., POPESCU, I., EXESCIJ, V. The action of smoking on the cardio-
   rascular a,pparatus. Medicina Interna (Bucurestl) 13: 347-351, 1961.
SK. MACDONALD, J. C., LIDWELL, 0. M.. WIU~HT, E. A. Serum cholesterol, smok-
   ing and body build. A survey in the Royal Air Force, British Journal of
   Preventive and Social Medicine (London) 19: 111-114, July 1965.
S88. J~CDONOU~H, J. R., HA~IICS, 6. G., GABBISON, G. E., Sm, S. C., LICHT-
   MAN, M. A., HEFELFINQEE, D C. The relationship of hematocrlt to car-
   diovascular states of health in the negro and white population of Evans
   County, Georgia. Journal of Chronic Diseases (St. Louis) 18(3) : 243
   257, March 1965.

S89. MACK, R. E., NOLTINC& D. D., HMANCAMP, C. E., BINS, R. J. Myocardial
   extraction of Rb' in the rabbit. American Journal of Physiology (Wash-
   ington) 197(g) : 1175-1177, 1959.

590. >IArurusri, R. E., %.XIAAF, W. E. Cigarette smoking and medical attendance
   Prior to death. Archives of Environmental Health (Chicago) 13( 1) : 66-
   73, July 1966.

S91. Mm~s, J. W. Epidemiology of coronary disease in industrial workers. III.
   Absentee rates and parental disease histories. Archives of Environmen-
   tal Health (Chicago) 13 : 655-661, November 1966.

81


992. Mums, J. W., Ar.muux, M. J. Epidemiology of coronary disease in i.ndus-
   trial workers. II. Absentee rates and serum lipids. Archives of ~~
   vironmental Health (Weago) 13: 645;664 ~November 1986.
998. MILTON, A. 8. The effect of nicotine on blood glucose levels and plasma non-
   esterified fatty acid levels in the intact and adrenalectomised cat. &it-
  ish Journal of Pharmacology and Chemotherapy (London) 28: 250-2~2,
    1986.

894. &fOmKOF'F, A. M., P~asor~a, 0. A. The coronary Personality: A critique.
   Psychomatic Medicine (New York) 29(l) : 1-14, Jauuary-FebTnary 1987.
S95. MOEIUB, J. N., HEaDy, J. A, Rm P. A. B. Physique of London busmeu.
   Epidemiology of uniforms. Lancet (London) 2: 56Q-670, Sept. 16,l~g~.
S96. MOBBIS, J. N., EAOAX, A., PATTISON, D. C., GAiWuEB, M. J., RAvrLq P. A. B.
   `Incidence and prediction of ischaemic heart-disease in London busmen.
   Lane& (London) 2: 553-559, September 1886.
897. Mosns, D. C., POWEBB, D., SOUIFF, L. A. Glucose blockage of the increase
   in stroke volume produced by smoking. Circuiation ; Journal of the
   American Heart Association (New York) 29(8) : 8oo-824, June lQg4.
998. MULCAW, It., Hrcxur, N. Cigarette smoking habits of patients with core.
   nary heart disease. British Heart Journal (London) 28: 4Q4-4QS, 198s.
599. MULCAHY, R., HI~KEY, N. The role of cigarette smoking in the cauaatibn
   of atherosclerosis. Geriatrics (Minneapolis) 22(2) : 16%174, February
    1967.

5100. M~AEY, R., HICKEY, N. J., MAUBBB, B. J. The aetiology of coronary
    heart disease. in women. Journal of the Irish Medical Association (Dub-
    lin) Bo: 2%29, January X67.

5101. UUBPHY, E. A., MUSTAED, J. F. Coagulation tests and platelet economy in
    atherosclerotic and. control subjects. Circulation; Journal of the Ameri-
    can Heart Assoeiation (New York) 25:.11&125, January 1962.
5102. NAHUM, L. H. Smoking and thrombosis. Connecticut Medicine (New
    Haven) 29 ( 12) : S5W354, December 1905.
5103. NAUOHTOX, J. Assessment of physical performance of middleaged Ameri-
    can men. Medical Times (Manhasset) Q5(2) : -227, February 1987.
SlQ4. New studies cite multiple factors in heart disease. Lung cancer age not
    aKected by smoking. Southern Tobacco Journal (Winston Salem) 80(l) :
    5, January 1966.

SlQ5. OBEUMAN, A., DOLI, R. E., G~AYBIEL, A. Interdependence among some fac-
    tors associated with coronary heart disease. Pensacola, Fia., U.S. Naval
    Aviation Medical Center, U.S. Naval School of Aviation Medicine Mar. 5,
    1964. [Unpublished.] 16 pp.

SlQ0. Orrvra, N. F. Arterial disease and hypertension. Presymptomatic diagnosis
   of ischaemic heart disease. Proceedings of the Royal Society of Medicine
    (London) 59: llSQ-1184, November 1986.
5107. OLIVEB, M. F., STUABT-HABBIS, C. H. Present position concerning prevention
   of heart disease. British Medical Journal (London) 2(5472) : 1203-1208,
    Nor. 2o,lQ05.

5168. OSTEZLD, A. M. Are strokes preventable? Medical Ciinics of North America
    (Philadelphia) 51( 1) : RX-111, January 1967.
5109. OSTBAIVDEX, L. D., JB. Bundle-branch block. An epi&miolOgic study. Cimu-
    lation ; Journal of the American Heart Association (New York) 30(g) :
    872881, December 1984.

9110. PAFFEXBABGIEB, R. S., JR., NOTKIIV, J., Kaviu+~l~~, D. E., WOLF, P. A., TH~WC,
   M. C., LERAUER, E. J. WILLU~S, J. L. Chronic disease in former Copege
   students. II. Methods of study and observations on mortality from

82


   coronary heart disease. American Journal of Public Health and the
   Nation's Health (New York) 56(6) : 982971, June lQ65.
8111. PAr'iiuX~r& R. S., JB, WHLIA~SS, J. L. Mortality Prom stroke in former
   college students. Framingham, Mass. U.S. Public Health Service, Na-
    tional Heart Institute, Field Epidemiological Research Section. n.d.
    25 PP.
8112. PENTE(X) B., SHILLI~~~~D, J. The acute eKects of smoking on mYocardia1
   performance in patients with coronary arterial disease. British Heart
    Journal (London) 26 : 422+$198p.
~113. ~ERBIN, M. J., KEUT, L. H., BBONTI&TEWAICT, B. Smoking and food prefer-
    ences. British Medical Journal (London) 1: 387-888, Feb. 11, 1981.
611-l. PICK, R. The present state of knowledge about the prevention and therapy
   of atherosclerosis. Medical Clinics of North America (Philadelphia)
    51(l) : 97-104, January 1887.
8115. R&&z, W. The neurogenic metabolic factor in ischemic heart disease.
   Pathogenesis and prevention. Diseases of the Chest (Chicago) 46(2) :
    150-157, August 1%.
8116. RMB, W. Origin, prediction and prevention of ischemic heart disease.
   Japanese Circulation Journal (Kyoto) 29: 118-22, February 1965.
5117. RaaB, W., KBZYWANEK, H. J. Cardiovascular sympathetic tone and stress
    response related to personality patterns and exercise habits. A potential
    cardiac risk and screening test. American Journal of Cardiology (New
    York) 16(l) : 42-53, July1965.
~118. Rxm, D. D., Ho-, W. W., H~EBEELT, 8.. Rosx, G. A cardiovascular
    survey of British postal workers. Lancet (London) 1: 614-618, Mar. 19,
     1966.
8119. ROTH, O., Bxaxr, A., Wm, G. D. Long range observations in 58 young
    patients with myocardial infarction. The American Journal of Cardiology
    (New York) 19: 331-388, March 1967.
Sl20. SACKETT, D. L., WIHKIXBTEI~, W., JB The relationship between cigarette
    usage and aortic athersclerosis. [Unpublished.]
Sl21. SALATIOH, J. S. Cerebral symptoms due to cigarette smoking. Journal of
    the Louisiana State Medical Society (New Orleanu) 117(7) : 227-229,
    July 1965.
s122. SOHIzvmmux, H. EiYects of nicotine and other alkaloids on storage and
    release of biogenic amines. In: Third World Tobacco Scientific Con-
    gress Proceedings. Held at the University College of Rhodesia and
    Nyasaland, `Saliabury, Southern Rhodesia, Febmary 1846, 196%
    Pp. 626-643.
S123. SCHIUUEB, W., NEFF, C. Rauchgewohnheiten und Herzinfarkt. Munchener
    Medizinische Wocheuscbrift (Munchen) 108: QQ3-915, lQ66.
Sl24. SCHW~BTZ, D., TOUCH, J., AMOEBA, G., BEAUMOITT, J. L., LE~PEGIBE, J.
    Tobacco and other factors in the etiology of ischemic heart disease in
    man : Results of a retrospective survey. Journal of Chronic Diseases (St.
    Louis) 19(l) : 85-55, January 1968.
WE. SH~IW S., WEINBLATT, E., FBANK, C. W., SAOEB, R. V. !L%e HLP.
   Study of incidence and prognosis of coronary heart disease. Preliminary
   findings on incidence of myocardial infarction and angina. Journal of
    Chronic Diseases (St. Louis) 18(6) : 527-558, June 1965.
S123. SHum, S., WEIRBL~TT, E., FBANK, C. W., SAQEB, R. E., DEBSEN, P. M.
   The H.I.P. study of incidence and prognosis of coronary heart disease:
   methodology. Journal of Chronic Diseases (St. Louis) 16 : 1281-1292,
     December 1968.


5127. Smoking and atherosclerosis. British Medical Journal (London) 1: 7&
    756, Mar. 26,1Q66.
5128. Smoking and blood clotting. British Medical Journal (London) 1: 83&
    834, Mar. 3O,lQ63.
5129. SOUBBI& A., HOUSSAY, H. E. J., BABOUS~E, A., ROTONDA& D. G. C., CnavrSo,
    0. A. Acci6n de1 fumar sobre la drculad6n menor. Prensa M&dicta Ar-
    gentina (Buenos Aires) 52 : 1847-1850, Aug. 27,1Q65.
S130. SPBA~E, H. B. Environment in relation to coronary artery disease. Archives
    of Environmental Health (Chicago) 13( 1) : 4-12, July lQ66.
5131. STALLONES, R. A. V. Prospective epidemiologic studies of cerebrovastm-
   lar disease. In: U.S. Public Health Service. Cerebrovascular disease,
   epidemiology, a workshop. Washington, U.S. Government Printing Of&,
    Public Health Monograph No. 76. Public Health Service Publication
    No. 144l,1@36. Pp. 51-55.
5132. Sruau, S., Ba~rm, K. EKect of chemoreceptor stimulation on the pulmonary
   veins. American Journal of Physiology (Washington) 210(3) : 535-53~,
     1966.
8133. TALJX~XT, G. D. In5uence of environmental factors on lipid-respouse curves.
    Cigarette smoking, salt, alcohol, and high-fat diet edlecting healthy males.
    Geriatrics (Minneapolis) 19 (8) : 575-584, August 1964.
5134. THOMAS. C. B., MUBPHY, E. A. Circulatory responses to smokiug in healthy
    young men. Annals of the New York Academy of Sciences 90: 266-276,
     1960.
5135. THOMAS, C. B., Boss, D. C., HIQIXB~THOM, C. Q. Precursors of hyperteu.
    sion and coronary disease among healthy medical students : Discrimi-
    nant function analysis. 1. Using smoking habits as the criterion. Bulletin
    of the Johns Hopkins Hospital (Baltimore) 115: 174-194, lQ64.
5136. TSUJIUOTO, A., TANINO, S., KUBO~OCHI, Y. EKect of nicotine on serum
    potassium and blood glucose. Japanese Journal of Pharmacology (Ky-
    oto) 15 : 415-422, December 1965.
Si37. U.S. Pumm HUCTH SEBVICE. Framingham, Mass. Duration of cigarette
    smoking habit. Myocardial infarction or coronary mortality. Heart
    Disease Epidemiology Study, Framingham, Mass. Apr. 2l, 1968. [Unpub-
    lished.] 4 pp.
9138. U.S. PUBLIC HEALTH SEWICE. National Center for Health Statistics.
    Cigarette smoking and health characteristics, United States July lQ64-
    June 1965. Washington, U.S. Department of Health, Education, and
    Welfare, Vital and Health Statistics Series 10, No. 34, Public Health
    Service Publication No. 1660, Muy 1987.64 pp.
8139. VI~WANATHAN, R. Acute eKeets oi cigarette smoking on pulmonary artery
    pressures. Indian Journal of Medical Research (Calcutta) 53(6) : 4%
    427, May 1965.
9140. W~~uua, A. R. P. (Editorial). The prevention of coronary heart disease.
    American Heart Journal (St. Louis) 72(6) : 721-724, December 1988.
5141. WALKEE, W. J., Gasooa~ros, G. Myocardial infarction in young men.
    American Journal of Cardiology (New York) 19 : 339343, March 1981.
9142. WEINSTEIN, B. J., EPSTEIR, F. H. Comparability of criteria and methods
    in the epidemiology of cardiovascular disease. Report of a survey. Cir-
    culation ; Journal of the American Heart Association (New York)
    30 ( 5) : 843-653, November 1984.
8143. WENZEL, D. G., STABK, L. G. EKect of nicotine on cardiac necrosis induced
   hy isoproterenol. American Heart Journal (St. Louis) 7l(3) : 368-370,
     March 1988.


sl~. Wxxsxn, D. G., SW, J. L. Interactions of nicotine and tyramine with adre-
   nergic blocking agents on ventricle strips. Archives Intemationalee de
    pharmacodynamie et de Therapie (Gand) 162(l) : 18Q-185, July 1966.
~145. \vEN53% D. G., TUBNE~, J. A., JOBDAN, 8. W., SI~~H, J. Cardiovascular
    interaction of nicotine, ergonovine and hypercholesterolemia in the rab-
    bit. Circulation Research (New York) 9 : ta%6QQ, May 1961.
8146. Wxxa~~, D. G., Ttm~~xa, J. A., KISSIL, D. EKect of nicotine on cholesterol-
    induced atherosclerosis in the rabbit. Circulation Research (New York)
    7 : 25f3-261,1959.

~14;. I~NZEL, D. G.. WATTAN~P~NQSIBI, A., Vnrrrar, D. Nicotine and renal
   hypertension in the rat. Journal of Pharmacology and Experimental
    Therapeutics (Baltimore) 145( 3) : 315-816, September 1964,
8148. WEST, J. W., GAZXA~, 5. V., B~~L-L.ET, S. Cardiac eKects of intracoronary
    arterial i&&ions of nicotine. Circulation Research (New York) 6:
    389-i%, May 1958.

8149. ~vESmAr& T. 0. Tobacco aikaloids and the release of catecholamines.
    In: Tobacco Alkaloids and Related Compounds. Proceeding8 of the 4th
    International Symposium held at the Wenner-Gren Center, Stockholm,
    February 1964 : 1985. Pp. 179-203.

8150. WEY, W., PFALTZ, C. R. Schlldigungen der Schieimhaut aer oberen luft-
    und Speisewege durch tabac. Praxis (Bern) 55: 182-184, February
     1966.

8151. WHITE, H. J., Goax, I., LABX~Y, B. J. The antagonism between nicotine
    and mucopolysaccharide activity. Biochimica e Biologia Sperimentale
    3(4) : 107-116,1964.

8152. WILENB, S. L. Relation of tobacco smoking to cardiovascular disease.
    National Conference on Cardiovascular Diseases (Washington) 2:
    360-361,1964.

6153. WILENS, 5. L., PLAIB, C. M. Cigarette smoking and arterioscierosis.
    Science (Washington) 138 : 875-877, November 1962.
S151. WILLIAM-OLSSON, L. R6kning och blodsocker. Lakartidningen (Stockholm)
    62 : 38103811, November 1966.

S165. WINEELSTZIN, W., Ja. Study of blood pressure in BuKalo, N.Y. Annals of
    the New York Academy of Sciences107 : 57&575, May 22,1Q63.
S156. WINKELSTELN, W., Jr. IV. Some retrospective &dies of cerebrovaacular
    disease. In: U.S. Public Health Service. Cerebrovascular disease,
    epidemiology, a workshop. Washington, U.S. Government Printing
    OKlee, Public Health Service Publication No. 1551, 1966. Pp. 41-49.
SGi. WYNN, A. The recognition of coronary proneness. Medical Journal of
    Australia (Sydney) 1: 350353, February 1967.
SlBS. YAMAXOTO, K., MUBA~I, T., FUKCI, I. Determination of inhibitors of
    urokinase-activated iibrinolytic ensyme system by means of gel filtra-
    tion in human semm. Keio Journal of Medicine (Tokyo) 15(2) :
    63-66, June 1966.

8159. ZEJ~~L, E. V. Pharmacological study of the central nicotinesensitive
   choiino-receptors. Activitas Nervosa Superior (Praha) 8(l) : 6&67,
     1966.

85


CHAPTER 2

Smoking and Chronic Bronchopulmonary
       Diseases (Non-neoplastic)

CONTENTS

Introduction _____ - ____ ---_-- ____________ --- _____ - _______
Chronic Bronchopulmonary Disease Mortality--------------
Chronic Bronchopulmonary Disease Morbidity- _ _ _ _ _ _ _ _ _ _ _ _
Studies Relating Smoking to Respiratory Symptoms- -- _ _
  Studies Relating Smoking to Pulmonary Function- - _ _ - - - _
Relation of Smoking to Heredity or to Constitutional Factors--
Pat,hologyStudies----- ____ - ___________ ---- ____ -_--__---_
AnimalExperiments ____ --_-- __________ ----- _____ - _______
CiliatoxicEffectsofCigaretteSmoke ____ ----- _____ --___--_
Other Factors Associated with Chronic Bronchitis or Em-
physemaorBoth_------..-- __________ -----___-_-___---_
  Additional Considerations Regarding Smoking and Em-
   physema---_____--__-----------------------------
CYted References--- __________ --__--_- ___________________
Sqplemental References------------------ _______ - _____ -_

PW
89
90
96
96
99
101
104
106
107

108

110
111
117

2-U-384 O-W-7

87


INTRODUCTION

PURWSE OF THIS RE~RT

This report reviews additional pertinent data relative to smoking
and chronic bronchopulmonary diseases-specifically chronic bron-
&tis and pulmonary emphysema.
The reader is referred to the Surgeon General's 1964 Report (68)
and recent textbooks for background information on the chemistry of
tobacco smoke, the metabolism and toxicity of specific components
of tobacco smoke, the physics of its retention in the air passages and
the lungs, and the mechanics of pulmonary function.

DBFINITIONS

The scope of this chapter will be limited to emphysema and chronic
bronchitis and it may be useful to present definitions of both terms.
There have been many definitions of chronic bronchitis and emphy-
sema. Those used in *he Surgeon General's 1964 Report had been pro-
posed by the American Thoracic Society in 1962 (37). With the in-
creasing public health interest in chronic bronchopulmonary disease,
attempts have been made to develop precise definitions to ca.tegorize
these diseases and to isolate them satisfactorily from other pulmonary
conditions. ,4 task force sponsored by the Chronic Respiratory Dis-
eases Control Program of the Public Health `Service and the National
Tuberculosis Association deliberated this together with related prob-
lems for a week in October 1966. They adopted the following
definitions (71) :

"Chronic bronchitis is a clinical disorder characterized by excessive
mucous secretion in the bronchial tree. It is manifested by chronic or
recurrent productive cough. The diagnosis of chronic bronchitis can
be made only if other bronchopulmonary or cardiac disorders are ex-
cluded a~ the cause for t.hese symptoms. The predominant pathologic
cllange is hypertrophy and hyperplasia of the mucous secreting glands
in the trachea and bronchi.

Pulmonary emphysema is an anatomic alteration of the lung
characterized by destruction of alveolar walls a;ccompanied by abnor-
mal enlargement of the air spaces distal to t.he terminal, nonrespira-
tory bronchiole."

These definitions will be used to describe chronic bronchitis and
emphysema as understood in the present report. They are being used

89


to emphasize the lack of progress in defining the two conditions for
purposes of differentiating them from other diseases of the lung. I&,&
erence may be made to the Surgeon General's 1964 Report where near-
ly identical definitions will be found.
SInability to distinguish between chronic bronchitis and emphym
has hampered medical research and exchange of information. The
P.H.S.-N.T.A. task force report states further :
"Although patients having only chronic bronchitis tend to have
more cqugh and sputum than do those having only pulmonary emphy-
sema, the array of symptoms, physical findings, and pulmonary
physiologic abnormalities are similar in ,both diseases.
"Chronic bronchitis and emphysema coexist in many patients * * $9'
This statement may help to explain some of the di&ulties encoun-
tered by research workers in studying these diseases and why the re-
searchers are limited to describing symptoms and signs observed in &e
populations under investigation. It may also explain why it is di.&
cult to distinguish these conditions in the present report which seeks
to record the research findings related to smoking and chronic respira-
tory disease published since the Surgeon General's 1964 Report. Re-
search findings on both diseases are not considered separately in this
report but are grouped in population studies, pathology studies, and
animal experiments. Additional considerations pertinent to pul-
monary emphysema nre then provided.

CHRONIC BRONCHOPULMONARY DISEASE
         MORTALITY l

Mortality from chronic bronchopulmonary diseases has continued
the upward trend well established at the time of publication of the
conclusions cited in the Surgeon General's 1964 Report. Deaths in the
United St,ates from emphysema or chronic bronchitis or both have
risen steadily from about 3,000 in 1950 to more than 20,000 in 1964,
as can be seen in table 1.

TABLE l.-Mort&ty from emphysema ad/or chronic bronchitis (IN2

    Year      Number
            of deaths
--         --

1964 _________ 20,208
1963 --------- 19,443
1962---------  15,915
1961_________ 13,302
1960 _________ 12,426

-

YW

NUmbI
of deaths

Year

Number
of desths

.-

1959 ---__ -___  10,433  1954- - - - - - - - -   4,877
1958- _ _ _ _ _ _ _ _   9,328   1953- - - - - - - - -  4,657
1957-- -- ___ __   8, 136  1952 --------_ 3,846
1956- - - - - - _ - _   6,535   1951- _ - -- - ---  3,660
1955---------   5,616   1950- - - - - - - - _   3,157

SOWCB: Vltaf Statfstl*l of the Unftad States. 1esCrli (70).

' AlI death rates throughout this chapter are per 100,000 population, unless
otherwise indicated.

90


The increase and aging of the population during the same period
does not account for this rise. Age-adjusted mortality rates for emphy-
sema without mention of chronic bronchitis increased about ten times
for men, from 1.3 per 100,000 in $950 to 12.6 in 1964. A similar, al-
though perhaps somewhat less drama.tic, increase occurred among
1vomen, from 0.2 per 100,000 in 1950 to 1.6 in 1964. Death rates from
chronic bronchitis rose less precipitously, doubling during the same
period (69,70). How much of this increase is the result of improved
diagnosis of these diseases and how much the result of a true change
in mortality patterns cannot be determined at the present time. Asso-
ciations have been demonstrated between these conditions and
smoking.

POPULAlTON 6TDolES

Included in this broad category are investigations that collected
information from ma group or groups of persons either by a series of
questions, by some form of physical examination, or by a review of
recorded data such as hospital records and death certificates.

PRosPzcTrvz STuDIzs

In the Surgeon General's 1964 Report, Endings from seven prospec-
tive studies were presented. Additional data have .been reported from
four of these investigations in the past 3 years. Information relevant
to smoking and chronic respiratory disease will be summarized here.

The study of mortality among policyholders of U.S. Government
Life Insurance policies available to persons who served in the Armed
Forces between 1917 and 1940 was initiated in 1952. Almost all the
293,658 policyholders were white males. Recently Kahn (&) pub-
lished a report that included all deaths from July 1954 through De-
cember 1962, a period of S+$ years.

The relation of cigarette smoking to death from bronchitis and
emphysema is presented by mortality ratios in table 2 and by specific
risk of mortality in table 2A. Given the definitions previously cited,
t.hese tables also illustrate the difficulties in separating these diseases.
The first row of table 2 gives combined mortality ratios and the next
two rows give the same data in an attempt to delineate the specific dis-
eases. Mortality ratios are given by the number of cigarettes smoked
per day at the time the men were enrolled in the study. Mortality
from these diseases is much higher among cigarette smokers than
among those who never smoked and rises with the number of ciga-
rettes smoked daily. The ratios are much higher for emphysema alone
than for chronic bronchitis with or without mention of emphysema.
A similar study of veterans was begun in Canada in 1955. Answered
questionnaires were returned by nearly 78,000 men whose subsequent
mortality for a period of 6 years was recently analyzed by Best and

91


his associates (I$). Deaths from chronic bronchitis and emphysema
have been summarized in table 3 which gives mortality ratios by
the number of cigarettes smoked each day. Here, again, the mortality
is much higher among smokers .and is directly related to the number
of cigarettes smoked. The mortality ratios reported for both diseases
combined are similar to those reported for the U.S. Veterans study.
TABLE 2.-Age-adjuhd mortulity ratios for current smokers of &ret&
      only, by number of c@aret&s smoked o?u$y

Bronchitis or emphysema or both (500-502,
527.1)-----------------------_--_-____
Bronchitis with or without emphysema
(500-502) _ _ _ __ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ __ _ _ _ _ _ _
Emphysema (527.1) ________ -__- _________

I 1.0

1.0   3.6   4.5
1.0   5.3  14.0

4.6

10.0

SOUXCE: U.S. Vetemu study (4).

11.8   18.2

4.6 8.3
17.0 25.3

TABLE 2A.-Age-specijic a7112ual probabilities of death per lOO,ooO 1
    person-years, for current smokers of cigarettes only

Chronic bronchitis and/or
emphysema:

  Age 55 to 64- _ _ _____ ___ __
  Age 65 to74-------------
Chronic bronchitis:
  Age 55 to 64 ___________ -_
  Age 65 to 74- _ __ ___ __ ___ _
Emphysema without
bronchitis:

Age55to64 _____________
Age65to74 ____ -__-___-_

-
I

cigarettea smoked per day at entrance to study

h8_lomI
ormwr
smoked

2
10


1
5



1
5

l-g

12    32    30    39      29
66   100   141   322     113

5
15

5
22



27
78

5     5       4
30    46      23

?
52

26    34      25
111   276      90

le20

21-29

&lla%&d

1 Annual probabilititv of death at each single year of age were comb&d into IO-year age gxunp8 by m
weights proportional to the distribution of the U.S. male population in 1080. Not shown if less thm 50
person-years of observation at any single year of age in the l&year interval.
SOUBCE: U.S. Veterans study (44).

When ,the two diseases are separated, the ratios for emphysema in the
Canadian study are similar to those for chronic bronchitis in the
U.S. study, and the ratios for chronic bronchitis are similar to those
attributed to emphysema in the United States. This illustrates the

92


problems of definition and one of the difhculties of direct comparison
between studies, especially dPhen different countries are involved.

TABLE &-Age-adjusted mOd4dity ratios for 8?nokers of cigarettes only
       by number of cigarettes smoked daily

c8aSe Of death

Non-     1-Q    lo-20   21+
smokers
----

Bronchitis or emphysema or both _______ - ______    1.0 6.11 10.0' 10.4'
Bronchitis with or without emphysema (500-502)-    1.0 7.0 13.7   14.6
Emphysema (527.1)--- __ _ __ _ __ ___ ___ __ _ __ _ __ _    1.0 4.8 6.1 6.9

1 Calculated from (id).
SOURCE: Canedi81l &IlSiOnerS study (fa).

A study among British physicians, the first of the large prospective
studies, was started in 1951 with the sending of a short questionnaire
to the 59,600 registered physicians then resident in the United King-
dom. Usable replies were received from 34,455 men and 6,192 women.
Ten years of observation of mortality in relation to smoking was
recently reported for this population by Doll and Hill (99,30). Their
findings on mortality from chronic bronchitis as related to smoking
included emphysema and are given in table 4. Only the standardized
death rates were presented in the report but the mortality ratios have
been calcul.ated from them to offer an easier comparison with the
other two studies. Again, it is clear that mortality from these combined
diseases (no attempt was made to differentiate them in the published
report) is strongly and directly related to the amount of cigarette
smoking.

TABLE 4.-Standardized deuth rates, per 100,000 population, for
bronchi& and emphysema for male smokers of cigarettes only, by
number of cigarettes smoked daily

CaIlS8 of death

Cig8lBttes SIIl0ke.d per day 8t k?IltTanCe ta study
Nier 1 I-14 1 15-24 1 25-k
smoked

Bronchitis (including emphysema-502,
527.1)-------_--____________________ 5

Mortality ratios ____ __________ - ________    1.0


SOWCB: Study of British Physiclam (2#, 20).

34     64     106


6.8    12.8     21.2

93


The fourth of the prosp&ive studies is the largest. More i&r 1
million men and women living in 25 States were enrolled in t&
investigation in late 1959 and early 1960. A report of the fist 4 yam
of observation was published recently by Hammond (36) and mar-
tahty from emphysema and chronic bronchitis as related to smoking
is given in table 5. A slight departure from the usual assignment of
cause of death should be mentioned. When the cause of d&h was listed
as chronic bronchitis with emphysema, it was oombined with emphy.
sema alone. For this reason, the cause of death in table 5 is not quite
the same as any of the causes listed in the other tables. Again, it is
clear that mortality from these entities is related to smoking for both
men and women. This was the only one of the four studies with enough
women enrolled to provide meanlingful data.

TABLE &-Mortality ratios for deaths due to emphysema and bronchi
uiul emphysema for cigarette makers--men and women in 2 we
iP'UP8 t

Cause of death

Emphysema and bronchitis with emphy-
sema (502, 527.1) ______ - _____ - _______    6.5    11. 4    4.9    7.5 '

t Mortality ratios of nonsmoker In the above categories 8re 1.00 by definition.
' CaiCtllated ifom (ss).
SOUBCE: Hammond. E. C. (ss).

Before summarizing the data presented from these four investiga-
tions, two further points should be made. Excessive mortality was
largely confmed to cigarette smokers. The mortality ratio for chronic
bronchitis and emphysema, for pipe and cigar smokers combined, in
the U.S. veterans study, was only 0.99; in the Canadian study it was
about 1.6 (based on only nine deaths) ; in the study of men from 25
States it was 1.37; and among British doctors the standardized d&h
rate was 15 (compared with five among nonsmokers). Whatever may
be the relationship of p!ipe and cigar smoking to chronic bronchitis
and emphysema, it is clear that it is substantially less important than
the relationship of cigarette smoking.

In two of these studies, stopping cigarette smoking is seen to have
an effect on subsequent mortafity from chronic bronchitis and emphy-
sema. In the course of the followup of the British physicians
(39, 30) it was possible to estimate the number of years a man con-
tinued smoking after he had answered the initial questionnaire. For
chronic bronchitis the mortaltty rates at first increased after cessation
of smoking and later fell well below the rate for men who contirmed
to smoke. The death rate from chronic bronchitis, per 100,~

04


ex-smokers of 5 years or more was 37 compared with a rate of 59 for
sll other smokers. Similarly, in the study of U.S. veterans, the mor-
tality ratio for chronic bronchitis and emphysema was 10.1 for all
male current digarette smokers but only 7.6 for men who had stopped
smoking for reasons other than "Doctor's orders."

Wicken (76) made a study of lung cancer and bronchitis mortality
in Northern Ireland. During the 3 years, 1960-62, a total of 1,262 men
and 630 women, aged 35 years or more, were certified as having died
of bronchitis. For each of tihese persons a control was selected-the
uest person in the Register of the same sex and &year age group who
last resided in the same area and who died of a nonrespiratory ilhmas.
Personal interviews with relatives of the decedents were carried out
for about 94 percent of the subjects and controls to determine the
decedents' smoking habits. In addition, a random sample of about
1,500 households in Northern Ireland was selected and one member of
each household was interviewed to obtain detiils of the age, sex, smok-
ing habits, and other information on all adult members. This informa-
tion on all adult members was used to define the adult pop&&ion of
Northern Ireland in order to calculate death rates.

Bronchitis mortality for both men and women was associated with
smoking and directly related to the number of cigarettes smoked daily,
as seen in table 6.

TABLE 6.-Age-ddardized d&h rates per 100,000 popdatiun front
bronchitis for adults &5 years okI and over aa dated to smoking habits

               Number of cigarettes smoked daily         Smoker of-


        Nonsmoker     l-10       II-22       23s.     Cl ettes,
             Pi&F  p:glY

--

Men- _ __ (124)' 64 (245) 189 (300) 220 (168) 284   (62) 99 (289) 118
Women-- (490) 58 (57) 77 (20) 118 (15) 201 __________   (2) 165

' Figuraa in pmnthma show the number of deaths upon which the rates 818 based.
SOURCE: Wick'en study (75).

Using these data, Wicken applied the bronchitis death rates observed
among male non-smokers to all the male population of Northern Ire-
land and estimated that had these rates prevailed, only 45 percent of
the male deaths from bronchitis would have occurred.

Recent data from the four prospective studies and the one retrospec-
tive study all reveal, for men, an association between cigarette smok-

95


ing and mortality from bronchitis and emphysema. All report an
increasing gradient of mortality with an increasing amount smoked.
This was also true among women although only one prospective study
and the retrospective study included enough women to permit calcula-
tion of death rates. Mortality was consistently higher among cigarette
smokers than among men who smoked pipes or cigars. These are
consistent associations that might be expected if there were a causal
relation between cigarette smoking and these diseases. In addition, if
such a relationship exists, cessation of smoking should be followed by
a reduction in mortality. This did occur in the two studies that in-
cluded information on changes in smoking habits.
These data, then, strongly support the conclusion that cigarette
smoking is at least one of the causes of chronic bronchitis and
emphysema.

CHRONIC BRONCHOPlJLlMONARY DISEASE MORBIDITY

STUDIES RELATING SIEOIUNQ TO RESPIRATORY ~+ZMPTOM:~

Most surveys of chronic respiratory disease are confined to the fre-
quency of signs or symptoms of disease. The National Center for
Health Statistics, however, in interviews from July 1964 to June 1965,
asked about certain chronic conditions including chronic bronchitis
and emphysema (76). This was asked in a national sample of 42,966
households containing about 134,060 persons. After answering ques-
tions about health for himself and other members of the household, the
respondent was asked questions about their smoking habits. A strong
relationship was found between smoking habits and the presence
of chronic bronchitis or emphysema or both. This is presented in
table 7.

TABLE `I.-Ageadjwted prevaknce rates of chronic bronchitis and/or
tmphystma per 100 persons 17 years and over, by sex, and mokbg
status-numbe-r of &are&~ per day (heaviest amount)


                                         Preaelltsmokeln


                                         l-10      11-m    a+


Men ________ -_- ____ - _________ 1.0           1.1     2.3     3.3
Women ____________ - _________               1.6 4.0     6.5


loumx: National Center for Hedth 8tatMlca (7#, Ffg. EL

96


In another study of the epidemiology of persistent cough, Wynder
(80) and his associates evaluated the smoking habits, occupation, and
residence (urban or rural) in a male population comprised of 315
hospital patients in New York, and 315 in California, and of 239
Seventh-Day Adventists living in California who were not hospital
patients. Persistent cough was reported from 23 percent of the Ad-
l*entists (who do not smoke), 45 percent of the New York patients,
and 53 percent of the California patients. Coughing was more fre-
quently reported by cigarette smokers than by those who smoked pipes
or cigars, as shown in table 8. Inhalers also had a higher rate of
persistent cough and the rate of cough increased with smoking in each
age group. Wynder found no correlation between urban or rural resi-
dence and persistent cough. Analysis of the California group showed
a higher rata of persistent cough that was independent of the number
of cigarettes smoked.

TABLE 8.--Percent of men with persistent cough as related to smoking
                ?mbii%

Non-
smoker Tgt'     cigarettea only     Mixed
                           smoker
            l-10   11-m zo+
-- ----

New York patients- _ _ - _ _ _ __ ___ _--   14    33 -IT
                                45    46   67    51
California patients ________________ 22 30 45 74 74 66
Seventh-Day Adventists- _ _- _ __ -__ _   23 ________________________ ______

SOURCE: Wynder, E. L. et al. (so).

Deane and her associates (28) studied symptoms in relation to smok-
ing in a group of about 500 outside telephone workers over age 40
working in the San Francisco and Los Angeles areas. Symptoms were
reported on a modified version of the British Medical Research Council
questionnaire. Regardless of the definition of the respiratory symp-
tom-persistent cough and phlegm, persistent cough, phlegm, and
shortness of breath-it was consistently experienced by a greater pro-
portion of those who currently smoked cigarettes than those who did
not.
Co&es and his coworkers (@) also found among 1,584 postal work-
ers aged 40 or more (all employees of the Detroit Main Post Office),
that for every symptom-cough and phlegm, chronic phlegm alone,
wheezing, shortness of breath-the prevalence was two to three times
greater among moderate (15-24 cigarettes per day) and heavy (25 or
more) smokers t.han among those who did not smoke. These differences
in symptom prevalence were observed for both men and women but
"chronic bronchitis" was reported more often by men, which Coates
ascribes to their being heavier smokers. The prevalence of chronic


cough and phlegm among ex-smokers was no greater than in
nonsmokers.

Very few studies have been carried out to estimate the association of
morbidity and smoking in young people. Peters and Ferris (60) retro.
spectively tallied the number of visits to the clinic at the University
of Health Services for 1,623 Harvard students and 404 Radcliffe stu-
dents. Smoking information had been gathered on these students in
their freshman and senior years. Smokers made significantly more
visits to the clinic in total and for respiratory diseases in particular.
There was a positive correlation between years smoked and the num-
ber of respiratory disease visits.

111 contrast to most studies which select population groups, H&i
(&?) studied virtually the entire population, age 40-64, in a immune
in western Finland. Although it was a mostly rural population, some
industrial workers were included. More than 95 percent of the men
and women invited responded to the survey. Questionnaires (based on
that of the British Medical Research Council) and medical examina-
tions were completed for 730 men and 890 women. Only 18.7 percent
of the men were nonsmokers and 21.6 percent ex-smokers, compared
with 86.1 percent nonsmokers and 3.6 percent es-smokers among
women. Prevalence of both cough and phlegm production was signif%
cantly higher among smokers than nonsmokers as seen in tible 9.

TABLE O.-Percent of men and women with cough (3 months in the year)
and with phlegm (3 months in the year), related to smoking h&de

Cough:

Men__------------------.
Women--- ______ - _______ _

Phlegm:
  Men-__--_____--_-------~
  Women---------- ________

cigfmttes smoked pa day

l-14

31.5
10.4

38.0
10.4

15-24     25-b
--

40.8    42.4
(3 of 7 women
smoking 15+
cigarettes/day)

42.9 I   42.4
(4 of 7 women
smoking 15 +
cigarettes/day)

Non-
smokml

4. 1
4.5





10.7
5.9

EI-
smoti

8.5
13.3

17.7
13.3

8OUBCE: Fhhti, E. (4.3).

In all the prevalence studies that have been identified and reviewed,
significantly more cigarette smokers consistently reported having
symptoms related to chronic respiratory disease. This was true for
cough, production of phlegm, wheezing, and shortness of breath. It

98


was also true when the respondents were asked not about symptoms
hut about disease, that is, chronic bronchitis and emphysema, and in
one instance was reflected in the number of clinic visits for respiratory
diseases. Prevalence of these symptoms increased with the amount of
(*igarettes smoked. It was less among pipe and cigar smokers, and ex-
cigarette smokers among whom the prevalence, in some reports, ap-
proached that of nonsmokers.

STDDIES RELATING SMOKING TO PULMONARY FUNCXION

Many of the surveys outlined in the previous section on respiratory
symptoms also included lung function tests as part of the examination,
Huhti (43)) for example, in his Finnish study took chest X-rays and
collected information on vital capacity, l-second forced expiratory
volume ( FEV,.o) and peak expiratory flow (PEF) as shown in table
10.

TABLE 10' .-Mean mlues of lung junctiom tests among men and women

by smoking h&its

Cigarettes smoked per day

-
I

                          I-14
--

FEVI .O (liters) :
  Men_-----_-___-_---_----   3.17
  Women _____________ - ____   2. 74
FVC (liters) :
  Men------------- ___-___   4.40
  Women _____________ _-___   Z53
PEF (c.c./sec.) :
  Men_--__---___-___-___-_    518
  Women- _ _ ____ ____ _______    431

IS-24     25+
--

4.51    4.26
(`1 (3

537    517
(9    (`1

Non-     ES-
SlIlOkf?l3   ElUOkerS

---

3. 46    3. 39
2. 42     2. 32


4.40    4.51
3. 18    3. 19


569     551
410     403

1 Although this table presents data for all ages combined, the same dilferences were apparent in each
byear age grouping.
2 Only 7 women smoked 15 or more cigarettes per day.
SOURCE: Huhti, E. (@).

Among men the FEV,, value was lower for smokers than nonsmok-
ers. The PEF value was slightly lower, but the vital capacity was un-
related to smoking. In this study none of the values seemed to be clear-
ly related to the number of cigarettes smoked. *4mong the relatively
small number of female smokers in this study, most of whom smoked
between one and 14 cigarettes per day, almost all the lung function
values were better than in the female nonsmokers. Female smokers
were slightly taller in height and slightly lighter in weight than fe-
male nonsmokers, which may account for this finding. However, fe-
male ex-smokers had slightly reduced FEVl.o and PEF when compared

99


with female nonsmokers, similar to the relationship noted between
male ex-smokers and male nonsmokers.

Coates (90) observed no relation between smoking habits and vital
capacity or FEV1., He did find, however, that the ratio of FEV&
VC was significantly lower among heavy smokers (25 or more ciga-
rettes per day) than nonsmokers. This was found for all but the old-
est group of workers, but here the number of subjects was small.

Peters and Ferris (69) asked 133 Harvard College seniors to com-
plete a questionnaire on respiratory symptoms and to perform some
sample tests of pulmonary functions. Of these, 124 responded. Wha
classified by smoking history, the smokers were found to record more
frequent cough, phlegm production, breathlessness, and wheezing with
or apart from colds. There was no difference in vital capacity be-
tween smokers and nonsmokers. Although the forced expiratory vol-
ume in 1 second (FEV1.o) was less for heavy smokers than nonsmok-
ers, this was not significant by itself. As a ratio of vital capacity this
did show a significant decrease. The air flow rate using the Wright
peak-flow meter and other flow rates determined from tracings of the
Stead-Wells spirometer' (FRla%, FR,%, FRZa%, FRlo%) did show
statistically significant reductions in heavy smokers as compared to
nonsmokers. These data show that relatively young cigarette smokers
have some impairments of ventilatory function, in turn suggesting
the possibility of a rather immediate effect of cigarette smoking on
respiratory symptoms and ventilatory ,function.
A series of experiments has been done by Krumholz and his asso-
ciates (49, 50, 61) to evaluate cardiopulmonary function in young
apparently healthy persons. The first experiment (~$9) involved 18
house staff physicians ranging in age from 24 to 37 years. Nine had
smoked at least one pack of cigarettes a day for the preceding 5 years
and nine had not smoked for at least the same time period. Extensive
pulmonary function studies were done at rest and after exercise. The
smokers were found to have a greater oxygen debt after exercise, de-
creased diffusing capacity at rest and with exercise, and decreased
total lung capacity and vital capacity.

In the second Krumholz experiment (50) 10 young staff physicians,
all of whom had smoked at least one pack a day for 5 years, were given
pulmonary function tests immediately after and again 3 weeks after
abstinence from smoking. Six physicians refrained from smoking for
G weeks and were tested again. After 6 weeks of no smoking, expira-
tory peak flow and pulmonary diffusing capacity were significantly
increased. Heart rate and oxygen debt after exercise were decreased.
After 6 weeks functional residual capacity was decreased and inspira-
tory reserve volume and maximal voluntary ventilation were
increased.

100


The final study (51) ag ain used 20 young medical persons divided
alnong 10 smokers and 10 nonsmokers. The mean pulmonary com-
pliance was significantly greater for the nonsmokers than for the
smokers.
Since cigarette smokers have a chronically elevated carboxy-
hemoglobin level, usually greater than 2 percent and occasionally
exceeding 10 percent, a study (19) was performed ha.ving nonsmokers
inhale enough carbon monoxide to raise their carboxyhemoglobin
levels to the range seen in a control group of cigarette smokers. This
lnaneuver caused the development, in the study group of nonsmokers,
of an increased oxygen debt with exercise and a reduced pulmonary
diffusing capacity at rest. These changes after carbon monoxide in-
halat.ion were similar to those found without carbon monoxide in-
halst,ion when comparing cigarette smokers to nonsmokers. (Further
data concerning smoking and carbon monoxide is presented and dis-
cussed in the chapter on cardiovascular diseases in this report.)

Findings from various studies relating smoking to pulmonary func-
tion are less consistent for certain criteria of measurement than from
t,hose relating smoking to respiratory symptoms. They are, however,
consistent in that they all report some form of diminished pulmonary
function among cigarette smokers, even when relatively young smokers
were studied. This is true of the studies outlined here as well as others
that have not been included (18, @, 56,58,81).  The usual measure-
ment found to be lower among smokers is the l-second forced expira-
tory volume (FEV l.O) either alone or as a ratio of the vital capacity
(FEVJVC). Vital capacity alone was generally not found to be
associated with smoking but diminished flow rates, such as FR,a, FRso
and the peak expiratory flow (PEF), were often observed. In these
studies, distinct quantitative ,relationships were not observed between
impairment of pulmonary function and the number of cigarettes
smoked daily.

RELATION OF SMOKING TO HEREDITY OR TO CON-
          STITUTIONAL FACTORS

Although various surveys and studies consistently show an associa-
tion between smoking and respiratory symptoms and mortality from
respiratory disease, there have been objections to interpreting this
relation as causal. Arguments have been made that smokers and non-
smokers may differ in some respects, perhaps biological, that are
relevant to the occurrence of disease. Others have suggested that pre-

101


dispositions to smoking and respiratory disease may have a common
genetic basis.
One method of trying to estimate the importance of heredity and
constitutional factors is to study the effect of tobacco smoking among
pairs of twins, particularly identical (monozygotic) twins. If, when
one twin in each pair of monozygotic and dizygotic twins is a smoker
and the other is not, an excess morbidity does not appear among the
smoking twins, it would seem that the exposure to tobacco smoke was
insufficient to result in greater morbidity. Cederlof and his associates
(26, 17) in Sweden studied smoking in relation to morbidity from
various causes among 12,889 pairs of twins. Replies to a mailed ques-
tionnaire dealing with smoking habits and residential ,history were
received from 10,947 pairs (85 percent). Replies to a second question-
naire with medical questions were received from both members of
9,319 pairs-another response of about 85 percent.
A subject, who answered "Yes" to the question, "DO you redarly
have a cough?" was *regarded as having "cough." If, when asked, "For
how many consecutive months a year do you have a cough?," the
subject checked more than 3 months, he ww regarded as having
"bronchitis."

If the group comprising only one of each twin pair (the fi? twin
on the twin registry) is considered as a random population, the as-
sociation observed between smoking and respiratory symptoms is given
in table 11.

TABLE 11 .-Prevdeme of "cough" and "bronchitie" among smokers and
         no-nemokere by eex and age

Sex and birth year

Men:

  1886-95---_-__---_--_---.
  1896-1905---------------.
  1906-15----- __-- -- ------_
  1916-25- - - - - - - - - - -- -- - -- -
Women:

17.7
15.5
15.0
13.8

1888-95___-__-____--_----     25.0
1896-1905- ___- ___________     18.0
1906-15------ _---_------_     14.2
1916-25 ___________ - __-- -.     11.1

i

-

smoke2

Nonsmoker

17.8
6.6
5.5
3.5

8.7
7.0
5.5
3.8

7.4
6.7
6.3
4.9

8.3
8.0
4.8
2.9

--

5.5
2.5
1.6
1.2

2.7
2.4
1.4
.6

SOUECE: Cedwlof# R., et d. (II).

These findings are similar to those previously reported for various
populations. These respiratory symptoms were then analyzed among
twin pairs with discordant smoking habits, that is, one twin of the

102


pair never smoked and t.he other either had smoked or still smoked at
the time of the survey. The findings are presented in table 12.

TABLE 12.-Preualence of "cough" and "bronchit~" among smokers and
     nonsmokers in smoking-discordant twin pairs

               Cough        BKOllChltis
Twin8

Smoker   Nonsmoker   Smoker   Nonmuoker Nsof

Monozygotic:

  Men-----------------
  Women-- ___ _____ -___
Dizygotic:

14.6      7.7    6.6      1.1     274
13.6      7.6    3.0      2.3     264

Men-----------------   12.3      5.5    4.5      1.8     733
Women- _ _ _ _ _ _ _ _ _ _ _ _ _   14.5      5.7     5.5      1.8     653

SCUECE: CeddOf, R., et 81. (17).

This table shows that the prevalence of respiratory symptoms was
much higher among the smokers in twin pairs than the nonsmokers.
The authors concluded that this hypermorbidity "speaks in favor of
a causal interpretation."

In a further analysis of the data from monozygotic twin pairs with
discordant smoking habits, Cederlof and his coworkers (15) divided
the series into a "low risk group" in which the nonsmoking twin
did not have "cough," and a "high risk group" in which .the non-
smoking twin had "cough." The two groups of smoking co-twins
corresponding to the two nonsmoking risk groups had higher than ex-
pected prevalence rates. The observed value for smokers in the low
risk group, however, was only half that expected for nonsmokers in
the high risk group. This suggests that for some i7w%~~~& the
genetic influence may be more important than smoking in the devel-
opment of cough. But, because any high risk group is only a small part
of the population, the total genetic effect will be much smaller than the
effect of tobacco.

Lundman (53) made a detailed study of twin pairs in Sweden. Of
247 twin pairs asked to participate, 196 pairs were examined (80
percent), of which 92 were monozygotic and 104 dizygotic. All par-
ticipants were interviewed and examined without knowledge of their
smoking habits. All twin pairs were concordant with respect to urban/
rural residence and discordant in smoking habits. After estimation
of lifetime exposure to smoking, 30 pairs were considered concordant,
thus limiting analysis to 77 monozygotic and 89 dizygotic pairs. The
smokers in both groups of twins had significantly higher frequencies
of some respiratory symptoms, such as persistent cough and morning
phlegm, but not of other symptoms such as dyspnea, "day cough", and
"day phlegm". Smokers also had "an increase in the unevenness of

211-394 o-07---8                            103


ventilation measured by nitrogen washout, and an increase in aim-my
resistance as measured by dynamic spirometry."

Two recent studies (17,58) of populations of identical and fraternal
twins show that for some individuals in the populations studied
a genetic element appears to be of some importance for the development
of cough. However, the effect of smoking was clearly shown to be
much more important for most ofthe individuals in the total popula-
tions studied. One study (53) also clearly showed that smoking twins
more often had reduced ventilatory function tests as compared to
their respective nonsmoking twins.

These data provide strong confirmatory evidence that cigarette
smoking can cause chronic bronchitis; however, no inferences with
respect to pulmonary emphysema can be based on these data. Studies
such as these, when specifically designed to provide additional in-
formation about pulmonary function, may be helpful in evaluating the
relationship between cigarette smoking and pulmonary emphysema.

PATHOLOGY STUDIES

Very few papers relating. the gross and microscopic appearance of
the trachea, bronchi, and lung parenchyma to tobacco smoking have
appeared in the last 3 years. Auerbach and his coworkers have con-
tinued their analysis of bronchial tissues taken f&m 758 subjects (7)
and lung parenchymal tissue taken from 1,340 men (8). They pub-
lished a report (9) correlating findings in the bronchial ltree with
findings in the lung parenchyma of 267 men who were included in
both previous studies. They reported a high correlation between
fibrosis in the lung parenchyma and different abnormalities of the
bronchial epithelium, such as hyperactive glands, increased number of
cell rows in the ciliated epithelium, and increased frequency of cells
with atypical nuclei. As reported previously by and summarized in the
Surgeon General's 1964 Report, more frequent and more severe ab-
normalities were observed among cigarette smokers. Sections of the
bronchial tree among ex-smokers were more like those of nonsmokers
while fibrotic changes in the lung parenchyma were more like those
observed among smokers.

Changes in the bronchial tree similar to those described by Auer-
bath and his coworkers were reported in a series of 100 random adult
autopsies by Hernandez and Anderson and their associates (38). They
reported a higher frequency of abnormal epithelial hyperplasia, goblet
cell hyperplasia, round cell infiltration, congestion, and edema in

104


bronchi from smokers than nonsmokers. There was, however, no evi-
dence of more bronchial gland hyperplasia.

These same workers also studied macroscopic sections of single lungs
from 211 routine autopsies on adults (I, 8). Analysis was limited to
165 of these cases on whom smoking histories were obtained, usually
from relatives. Without knowing the identity of the subject or his
smoking history, each lung section was classified on a sca.le from 0 to 6
by severity of emphysematous changes. The type of emphysema was
also described as panlobular (changes throughout the secondary pul-
monary lobules), centrilobular (changes located around the centers
of the secondary lobules), or mixed. The severity of emphysematous
changes was about the same for men and women, but for each sex,
changes were more severe among smokers than nonsmokers, as seen
in t.able 13.

TABLE 13.~Mean severity of emphysema clastS$ed by mamosedions by
          sex and smoking history

      Mean de
Numbfu %Z-
--

Men:                        Women :
  Smokers _______ _   96     2. 3     Smokers-- ______ 18     2.1
  Nonsmokers- _ _ -   11     1. 1     Nonsmokers---- 40 .3

SOUSCE: Andemm, A. E.. Jr., et al. (8.

Perhaps more important was the observat.ion that the type of pa-
thology seemed strongly related to smoking (9). Of 48 subjects whose
lung macrosections were classified as having mainly centrilobular
emphysema, 45 persons had been smokers. Ih contrast, the 62 subjects
judged to have panlobular emphysema were divided in t.he expected
proportions, 33 smokers and 24 nonsmokers.

Petty and his associates (61) also studied postmortem Endings in
the lungs of a series of 253 men over age 40, unselected for smoking,
who died in two Denver hospitals during a 6-year period from 1959 to
1965. The presence and severity of emphysema was estimated and
graded in four categories, from 0 to 3 + . During the last 3 years of
t.he study bronchi of 179 men were examined for mucous gland hyper-
plasia. Independent of the morphological studies, smoking histories
were obtained for each person, apparently `by questioning relatives,
although this is not clearly stated. Men were grouped according to the
amount of cigarettes smoked during their lifet.imes by calculating
pack-years of smoking. (One pack-year is the number of cigarettes
smoked if a person smoked one pack per day for a year. A pack-year
could also mean smoking two packs a day for 6 months, one-half pack
a day for 2 years, or any equivalent amount.)

105


Of the group of 179 individuals, 54 had mucous gland hyperplasia.
Of the 54 persons involved, 51 had smoked more than 20 pack-years,
and the remaining three were essentially nonsmokers. In contrast,
approximately one-third of the 125 men in whom mucous gland hyper-
plasia was not found were essentially nonsmokers. When the total
group of 253 men was studied for evidence of pulmonary emphysema,
114 were found to have moderate or severe pulmonary emphysema. Of
the 114, 98 had smoked more than 20 pack-years. The other six, who
essentially were nonsmokers, had either asthma, previous tuberculosis,
deep-seated lung infections or other demonstrable relationships with
previous pulmonary disease. In contrast, approximately one-third of
the remaining 139 men, who had either very mild or no emphysema,
were essentially nonsmokers.
Only one study has been found in which the frequency of abnormal
bronchial epithelial cells in living persons is compared with smoking
history. Robbins (63) studied a group of 103 college students between
the ages of 17 and 24. Of the 45 who had never smoked, atypical
epithelial cells were found in six (13 percent). This compares to 26
(45 percent) of the 58 students who had been smoking 10 or more
cigarettes daily for 1 to 8 years. Cytological examination was done
without knowledge of whether the specimen came from a smoker or
nonsmoker.

ANIMAL EXPERIMENTS

Results of two experimental studies relating smoke inhalation to
lung parenchymal changes in dogs have been published in the last
3 years. Hernandez and his coworkers (39) used 23 healthy greyhounds
retired from racing. Eight served as controls and 15 were exposed to
high concentrations of cigarette smoke ,for 30-45 minutes twice daily
in wooden inhalation chambers. Seven animals were exposed for ap-
proximately 5 months and the remaining eight were sacrificed after
almost 15 months of smoke inhalation. Disruption of the lung paren-
chyma was assessed macroscopically by comparison with preselected
standards graded in severity from 0 to 3. Assessment was made without
knowledge of the source of the lung specimen. Lung damage among
dogs that were exposed longer showed significantly greater disruption
of the lung parenchyma.
Auerbach and his associates (6,8) tracheostomized 10 adult .beagles
and, in an attempt to approximate human smoking more closely,
exposed them to cigarette smoke through the tracheostomy tube.
Five dogs died during this experiment and the remaining five were
sacrificed after approximately 14 months of exposure. Other beagles

106


mere kept as controls; two had tracheostomy openings. These control
do@ were sacrificed at the time the last five smoking dogs were sacri-
ficed. Lungs of the dogs exposed to cigarette smoke showed microscop-
ic&y the presence of dilated air spaces, especially beneath the pleural
surface. Here the alveolar septa showed a fibrous thickening of the
\valls with areas of rupture and dilated air sacs. Padlike attachments
to alveolar septa were found. These zones of connective tissue sur-
rounding dilated air sacs were also visible macroscopically as white
areas on the lung surface. There was no t.hickening of the walls of
small arteries and arterioles within the lung. The lungs of the control
dogs were normal in appearance with none of these changes. These
:tbnormalities approximate `but are not fully concordant with some
of the typical pathological tidings in human emphysema. This ex-
periment does indicate that inhaled cigarette smoke apparently can
damage the pulmonary parenchyma of dogs. Other findings (6) as
yet unpublished, indicate t.hat abnormalities of the bronchial epi-
thebum resulted that approximate many of lthe histopathologic flnd-
ings of human chronic bronchitis.
Rockey et al. (64) have noted that cigarette smoke produces bron-
chial and parenchymal changes in dogs that approximate some of the
histopathologic findings found in human smokers who have chronic
bronchitis and/or pulmonary emphysema. Mouzakis (57) has noted
similar changes in rabbits, and in dogs exposed to cigarette smoke
through tracheostomies.

Researchers carrying out pathological studies have consistently re-
ported epithelial hyperplasia of the bronchial tree associated with
smoking. They have also reported that fibrosis and emphysematous
changes in the lung parenchyma, although observed among non-
smokers, occur much more frequently among men and women who have
histories of smoking. Changes in the lung parenchyma, approximating
some of the changes noted in human emphysema, have also been pro
duced experimentally in dogs by exposure to cigarette smoke.

CILIATOXIC EFFECTS OF CIGARETTE SMOKE

The toxic effect of tobacco smoke on the ciliary defense mechanism
of the respiratory system has ,been confirmed by additional experi-
mental studies (9, 10, 13, 93, 24, $36, $7, 46, 47, 77, 78) which seek
to determine more exactly the mode of action of the ciliatoxic agents
contained in tobacco smoke. As yet, hydrogen cyanide and acrolein
appear to have the greateet ciliatoxic effects of the agents that have
been identified in the gaseous phase of tobacco smoke, although for-

107


maldehyde, crotonaldehyde, formic acid, acetic acid, proprionic acid,
and some phenols are also ciliatoxic (25, & 48, 73, 77, 79). Further
information may be obtained from a special symposium on ciliary
activity held in 1965 (48). A recent study ($22) suggests that oxidative
enzymes such as adenoeine triphosphatase, apparently important to
ciliary activity, may be adversely affected by cigarette smoke. Addi-
tional research is necessary before precise conclusions can be stated
concerning which, if any, of the identified ciliatoxic agents contained
in tobacco smoke are most damaging to the human respiratory system.

OTHER FACTORS ASSOCIATED WITH CHRONIC BRON-
      CHITIS OR EMPHYSEMA OR BOTH

It is not the purpose of this report to discuss all the factors that
may play a role in the development of chronic bronchitis and em-
physema. It is important, however, to recognize that these condi-
tions do exist among people who do not smoke and that many smokers
apparently escape all signs of aflliction. It is also important to recog-
nize that other factors have been associated with the development of
chronic respiratory disease, or chronic bronchitis and emphysema,
as we have defined chronic respiratory disease. We must be concerned
with the multiple etiology of .biological processes. One factor already
cited is the role of hereditary or constitutional factors in the develop-
ment of respiratory symptoms, either operating alone or in conjunc-
tion with other factors such as smoking.

Aside from the personal pulmonary pollution inherent in smok-
ing, occupational exposures (a wider form of pollution) and exposure
to various pollutants in the atmosphere have both been shown Ito influ-
ence the prevalence of respiratory signs and symptoms. Studies made
in some specific industries-for example, pulp mill workers in New
England (S$?), coal miners in West Virginia (31), and gold miners
in South Africa (66, 67)-ha ve shown an increased frequency of
respiratory symptoms or of diminished pulmonary function among
men exposed ito certain dusts and fumes.
These studies indicate that cigarette smoking is generally more
important than the occupational exposures in producing respiratory
disease in the workers. These studies also suggest that cigarette smok-
ing may interact with some occupational exposures to produce even
greater deleterious effects. Cigarette smokers outnumber by far the
workers subjected to unusual environmental exposures. Also, there has
been a general improvement in many occupational environments, in the
continuing effort to remove or reduce the exposure to specific indus-
t,rial air pollutants.

108


Climatic and meteorologic variations involved with differences in
quantity and quality of specific air pollutants make investigations of
atmospheric pollution very complex. There have been many studies,
however, attempting to examine the association of air pollution with
chronic respiratory disease. Often comparisons of mortality and mor-
bidity are made between urban and rural areas, assuming a difference
in air pollution but not measuring it directly. Wicken (75) in his
retrospective study of mortality from chronic bronchitis in Northern
Ireland found higher mortality rates with greater degrees of urbaniza-
tion. Air pollution was suggested as a factor.
Holland and Reid (43) compared the prevalence of respiratory
symptoms, sputum production, and lung fun&ion in London and in
three county towns. The London men had more and severer symptoms,
produced more sputum and had poorer lung function test results.
Smoking habits were shown to be closely related to respiratory dii-
turbance but urban-rural differences in these habits could not explain
the greater frequency of respiratory symptoms in London.

A Canadian study reported by Bates et al. (11) indicates that among
four cities studied, the city with the lowest amount of industrial dust-
fall and sulfur dioxide levels had the study group wit.h the lowest
prevalence of chronic bronchitis. Preliminary results also indicate
that this group had the lowest decline of pulmonary function. The
groups of males in each city were approximately concordant for
other factors, including the influence of cigarette smoking.

Ferris and associates (3, 4, 33) studied air pollution and its effect
on respiratory symptoms and functions in two separate towns-chilli-
wack, British Columbia, and Berlin, N.H. After standardizing the
data for age and cigarette smoking, they observed a correlation be-
tween symptoms of chronic bronchitis and the level of air pollution
as measured by the mean sulfation rate. They also found pulmonary
function tests to be better in Chilliwack when controlled for smoking
habits and age. This may be .associated with the lower level of air pol-
lution in Chilliwack.

Studies of populations of twins are especially valuable in assessing
the influences of constitutional factors and environmental considera-
tions, such as cigarette smoking and air pollution. Cederlof (14)) using
interview techniques on a large population of twins in Sweden, found
that compared with smoking, air pollution was of secondary im-
portance in causing respiratory symptoms indicative of chronic bron-
chitis and/or emphysema. In both the monozygotic and dixygotic
twins, again using the co-twin control method, individual variations
suggested that the propensity to develop cough from smoking also
may well be pertinent with regard to air pollution but that, when
considering the total population, individual variations appear to be
of minor influence (15).

109


Other studies (55,76) have suggested a relation between air pollu-
tion and symptoms or mortality from chronic respiratory disease,
although they were not controlled for differences in cigarette smoking.

The contributions of air pollution, industrial pollution, and personal
pollution have been summarized recently by Higgins (~$0). He con-
cluded, as we must from the available evidence, that all "* * * three
types of pollution are associated with increased amounts of respiratory
disease and respiratory disability. " All the recent evidence, however,
does not alter the conclusion in the Surgeon General's 1964 Report that
"the dominant association in the United States is between cigarette
smoking and chronic respiratory disease."

ADDITIONAL CONSIDERATIONS REGARDING SMOKING AND EBXPEYSEMA

This crucial question must be answered affirmatively before an infer-
ence can be made that smoking directly causes pulmonary emphysema :
Does inhaled tobacco smoke have a direct toxic effect on the alveolar
tissue in the lung parenchyma which is important in the pathogenesis
of pulmonary emphysema? At present, it cannot Abe answered.

If future evidence supports such a finding of a direct toxic effect,
we will have the missing link to the present chain of evidence showing
a strong association between cigarette smoking and many cases of
pulmonary emphysema and an inference of oausation may validly be
made. The available evidence that follows has only indirect pertinence
to the question.

The experiments of the Auerbach, Hernandez, and Rockey groups
support the thesis that there is a direct toxic effect of cigarette smoke
on the pulmonary tissue. Possibly this direct toxic effect, if proven to
exist, contributes to the rupture and fibrosis of the alveolar tissue.
However, in these animal studies there were also some differences from
the typical anatomic findings of human pulmonary emphysema.

Surfactant, a fluid substance lining the alveolar cell walls, appar-
ently is important for maintaining tissue surface tension and thus the
spatial configuration of the alveolar walls (65). Zn vitro abnormalities
have been noted in surfactant as a result of cigarette smoke (B, 74).
Alveolar macrophages (specialized cells that incorporate and remove
foreign material from the affected lung area) are reportedly damaged
in vitro by cigarette smoke (35). Abnormalities of the alveolar macro-
phages and lipophages with inhalation of cigarette smoke are also
reported (54) in cytological studies of human bronchial washings,
apparently reflecting damage in viva.
Studies (@,50,51,63) of the pulmonary function of relatively young
smokersand nonsmokers also indicate that abnormalities of pulmonary
diffusion noted in cigarette smokers, may, in part, be related to a
direct toxic effect of cigarette smoke. However, some of these abnormal-
ities are related to the unevenness of pulmonary ventilation associated

110


with airway abnormalities. Damage to the pulmonary arterial capil-
laries has frequently been noted on autopsy examination of smokers.
This damage may be a direct effect'of smoke inhalation and, function-
ally, may impair the vascular perfusion of the alveolar tissue, thus
leading to further deficiencies in alveolar tissue function.
The possibility must also be considered that the accelerated in vitro
thromhus formation (discussed in the cardiovascular chapter of this
report), associated with cigarette smoking, may be the basis for mul-
tiple small thromboses in the pulmonary arterial capillaries.

Additional research is also needed to answer questions concerning
other factors that may account for the apparent increased suscepti-
bility of some individuals to cigarette smoke, such that they have a
marked excess mortality from this disease. Genetic and constitutional
factors may be important to some individuals' development of pulmo-
JJaq emphysema, just as these factors appear to be important in the
development of cough in smokers, as reported by Cederlof and his
associates (1.4, 15,16, 17). An increased susceptibility of some indi-
viduals to the emphysema associated with cigarette smoking has been
suggested, but not proved, by the occasional reports of "familial"
emphysema (42, 529.

Other probable causes of pulmonary emphysema, such as allergic or
infectious disease processes, alsoshould be investigated for interactions
with and without smoking. Other apparent causes of pulmonary
emphysema, such as possibly atmospheric air pollution, may be inter-
`acting with cigarette smoking to produce effects even more deleterious
to human health.

The observation that other probable causes of pulmonary emphy-
sema may exist should not detract from the strong relationship that
has been shown to exist between cigarette smoking and pulmonary
emphysema. Further investigations of the mechanisms of injury to the
cellular and subcellular structures of the lung tissue are recommended
(34). Also, clarification of diaguosti'c nomenclature and criteria would
be helpful, as indicated in the earlier discussion of definitions in this
chapter.

CITED REFERENCES

(1) ANDEBSON, A, E., JB, HEENANDEZ, J. A., ECKE~T, P., FOBAKEB, A. G. Emphy-
  sema in lung macrosections correlated with smoking habits. Science
   (Washington) 144(382l) : 10254026, May 22,X%
(a) ANDEB~ON, & E., JB., HE~NANDE~, J. A., HOLMEB, W. L., F~RAXEB, A. G.
  Pulmonary emphysema. Prevalence, severity, and anatomical patterns
  in macrosections, with respect to smoking habits. Archives of Environ-
  mental Health (Chicago) 12 (5) : 569-577, May 1966.
(8) ANDEBsom, D. O., I@TUUE, B. G., JR Air pollution levels and chronic respira-
  tory disease. Archives of Environmental Health (Chicago) iO(2) : 307-
  311, February 1936.

111


(4) AHD~SOH, D. O., Foams, B. G.., JR, ZICKXA~TEL, It. The Chilliwack Itee-
  piratory Survey, 1963. Part IV. The e!fect of tobacco smoking on the
  prevalence of respiratory disease. Canadian Medical Association Jour-
   nal (Toronto) 9209) : 1966-1676, May 15,1965.
(5) AUEBBACH, O., H~laasoao. E. C., KIRMAN, D., G~amnxEL, L. Emphysema
  produced in dogs by cigarette smoking. Journal of the American Medical
   Association (Chicago) 199(4) : 241-246, Jan. 23,1967.
(6) AUEBBACH, O., HAMMOND, E. C., KIRMAN, D., GaBPIugEIs L., STOUT, A. P.
   Histological changes in bronchial tubes of cigarette smoking dogs. East
  Orange, N.J., U.S. Veterans Administration Hospital, March 1967. [Un-
   pubhshed.] 8 pp.

(7) AIJEBBACH, O., STOUT, A. P., HAMMOND, E. C., Gaa~~xxx~, L. Changes in
  bronchial epithelium in relation to sex, age, residence, Smoking and
  pneumonia. New England Journal of Medicine (Boston) 267(3) : 111-119,
   July 19,1962.

(8) AVEBBACE, O., STOUT, A. P., HAXYOND, E. C., GAEFISXIZ, L. Smoking habits
   and age in relation to pulmonary changes. Rupture of alVf?Olar septums,
  fibrosis and thickening of walls of amalI arteries and arterioles. New
   England Journal of Medicine (Boston) 269(29) : 1945-1654, NOV. 14,1963.
(9) A UEBBACH, O., STOUT, A. P., HAMMOND, E. 0.. GABFIR~EL, L. Interrelation-
  shfus among various histologic changes in bronchial tubes and in lung
  parenchyma. American Review of Respiratory Diseases (Baltimore)
   90 (6) : 8674376, December 1964.

(IO) BILLLEROEB, J. J., DAWEON, F. W., DIZRTJYTJI& M. G., H.krmno, H. B. Effects
   of nicotine on ciliary activity & vitro. Annals of Otology, `Rhinology and
   Laryngology (St. Louis) `74(2) : 303-311 June 1965.
(II) BATES, D. V., Goauon, C. A., PAUL, G. I., PLACE, R. E. G., S~KIIAL, D. P.,
   WOOLF, C. B. Chronic bronchitis. Report on the third and fourth stages
   of the coordinated study of chronic bronchitis in the Department of
   Veterans A&ins, Canada. Medical Services Journal, Canada (Ottawa)
   22(l) : l-59, January 1966.

(12) BEST, E. W. R., A Canadian study of smoking and health. Ottawa, Depart-
   ment of National Health and Welfare, 1966.137 pp.
(IS) CAB~OIV, S., GOLLUIAMEB, R., CABPENTEB, R. Responses of ciliated epithelium
   to irritants. Mucus transport in the respiratory tract. American Review of
   Respiratory Diseases (Baltimore) 93 (No. 3, Part 2) : 86-92, March 1966.
(14) CEDEBLOF, R. Urban factor and prevalence of respiratory symptoms and
   "angina pectoris." A study of 9,168 twin pairs with the aid of mailed
   questionnaires. Archives of Environmental Health (Chicago) 13 (6) : 743-
   748, December 1966.

(15) CEDE~I~F, R, E~roas, M-L, Famxno, L., JONSSON, E. Hereditary factors,
   "spontaneous cough" and "smoker's cough." A study of 7,869 twin-pairs
   with the aid of mailed questionnaires. Archives of Environmental Health
   (Chicago) 14(3) : 461+, March1967.
(16) CEDEBLOF, R., Fammm, L., JONSSON, E., KAIJ, L. Morbidity among monosy-
   gotic twins. Archives of Environmental Health (Chicago) lO(2) : 346-
   350, February 1965.

(17) CEDEULOF, R., Fmmm, L., JONSSON, E.., KAIJ, L. Respiratory symptoms and
   "angina pectoris" in twins with reference to smoking habits. An epidemic
   logical study with mailed questionnaire. Archives of Environmental
   Health (Chicago) 13 (6) : 726-737, December 1966.
(18) CHAPMAN, T. T. The acute effect of cigarette smoking on pulmonary func-
   tion Journal of the Irish Medical Association (Dublin) 56(333) : 72-74,
    March 1965.

112


(19) CHEVALIER, R. B., KBUYHOLZ, R. A., Ross, J. C. Reaction of nonsmokers
   ,to carbon monoxide inhalation. Cardipulmonary responses at rest and
   during exercise. Journal of the American Medical Association (Chicago)
   198( 10) : 1061-1064, Dec. 5, 1966.

(20) C%ATE~S, E. O., JB., Bownr, 0. C., REINSTEIN, N. Chronic respiratory disease
   in postal employees. Epidemiologic survey of a group employti in one
   building. Journal of the American Medical Association (Chicago) 191
   (3) : 161-166, Jan. 18, 1965.

(21) COOK, W. A., WEBB, W. R. Surfactant in chronic smokers. The Annals of
   Thoradc Surgery (Boston) 2(3) : 327-333, May 1966.
(33) caxss, H. R., SPOCK, A., HEATHEBINQTON, D. 0. The localization of succinic
   dehydrogenase, cytochrome oxidase and adenosine triphosphatase in
   ciliated respiratory epithelum. Journal of Histochemistry and Cyto-
   chemistry (Baltimore) 13 (8) : 677683, November-December 1965.
(2s) DALHAMN, T. Studies on tracheal dliary activity. Special reference to the
   effect of cigarette smoke in living animals. American Review of Respira-
   tory Diseases (Baltimore) 89 (6) : 870-877, June 1964.
(24) DALHAMN, T. Effect of dgnrette smoke on ciliary activity. American
   Review of Respiratory Diseases (Baltimore) 93(3) : 108-114, March 1966.
(~5) DALHAMN, T., LAQEFWTEDT, B. Ciliostatic effect of phenol and resorcinol.
   Archives of Gtolaryngology (Chicago) 84(3) : 325-328, September 1966.
(26) DALHAYN, T., RYLANDEB, R Ciliastatic action of smoke from fflter-tipped
   and non tipped cigarettes. Nature (London) 201(4917) : 401-492, Jan. Xi,
   25,1964.

(67) DALHAYN, T., RYLANDEB, R. Ciliastatic action of cigarette smoke. Varying
   exposure times. Archives of Otolaryngology (Chicago) 81(4) ; 379-382,
   April 1965.

(28) DUNE, M., GOLDSMITH, J. R., TUMA, D. Respiratory conditions in outside
   workers. Report on outside plant telephone workers in San Frandsco
   and Los Angeles. Archives of Environmental Health (Chicago) lO(2) :
   32531, February 1965.

(29) DOLL, R., HILL, A. B. Mortality in relation to smoking : 10 years' observa-
   tions of British doctors (pt. 1). British Medical Journal (London)
   l(5395) : 13991410, May 1964.

(SO) DOLL, R., HILL, A.B. Mortality in relation to smoking: 10 years' observa-
   tions of British doctors (concluded). British Medical Journal (London)
   l(5396) : 1460-1467, June 1964.

(31) EIUEZELINE, P. E. The effects of occupation on chronic respiratory disease.
   Archives of Environmental Health (Chicago) 14(l) : 18t%WO, January
    1967.

(38) Fxaars, B. G., JB~, Buao~as, W. A., WOBCESTEB, J. Prevalauce of chronic
   respiratory disease in a pulp mill and a paper mill in the United States.
   British Journal of Industrial Medicine (London) 24( 1) : 26-37, January
    1967.

(39) -IS, B. G., JR., WHITTENBEEQEE, J. L. Effects of community air pollution
   on prevalance of respiratory disease. New England Journal of Medicine
   (Boston) 275 (25) : 1413-1419, Dec. 22,19&X
(84) FLGTOHEB, C. M. Bumtows, J. B., NIDEN, A. H. American emphysema and
   British bronchitis. A standardized comparative study. American Review
   of Respiratory Disease (Baltimore) 96 (1) : 1-13, July 1964.
(35) GBJEEN, G. M., CABOIJN, D. The depressant effect of cigarette smoke on the
   in &ro antibacterial activity of alveolar macrophages. New England
   Journal of Medicine (Boston) 276(S) : 421+t27, Feb. 23, 1967.

113


(86) HAMMOND, E. 0. Smoking in relation to the death rates of 1 million men
   and women. In: Haenssel, W., editor. Epidemiological Approaches to the
   Study of Cancer and Other Diseases. Bethesda, U.S. Pub& Health
   Service, National Cancer Institute Monograph No. 19, January 1966.
   Pp. 127-m.

(87) H-IS, H. W., M~~EELY, G. R., Rxxmrrrr, A. D., Ja., Srxazn, J. D., WYArr,
   J. P. Definitions and classillcation of chronic bronchitis, asthma and
   pulmonary emphysema. American Review of Respiratory Diseases
   (Baltimore) 85(5) : 762-768, May 1962.

(58) HEENANDEZ, J. A., ANDFJMON, A. E., F~B.AKEB, A. G. Interrelationshipa be-
   tween bronchial alterations, emphysema in lung imu?roseCtiCMis, and
   smoking, Presented at the 62d rLnnua1 Meeting of the American Aseoda-
   tion of Pathologists and Bacteriologists, Philadelphia, Pa., Mar. 67.
   1965. [Unpublished.] 2 pp.

(89). HE~NA~DE~, J. A., ANDEB~OR, A. E., Ja, HOLMEB, W. L., FOEAKEE, A. G.
   Pulmonary parenchymal defects in dogs following prolonged cigarette
   smoke exposure. American Review of Respiratory Diseases (Baltimore)
   93(l) : 78-83, January 1966.

(#I) HIQQINS, I. T. T. Air pollution and chronic respiratory disease. ASHRAE
   Journal (New York) S(8) : 37-45, August 1966.
(41) HOLE, B. V., WA~~EBMAN, K. Familial emphysema. Annals of Internal Medi-
   cine (Philadelphia) 63 (6) : 1909-1017, December 1965.
(42) HOLLAND, W. W., Ram, D. D. The urban factor in chronic bronchitis. Lan-
   cet (London) 1(7383) : 445-448, Feb. 27,1965.
(49) HIJHTI, E. Prevalauce of respiratory symptoms, chronic bronchitis and pul-
   monary emphysema in a Finnish rural population. Field survey of age
   40-64 in the Harjavalta area. Acta Tuberculosea et Pneumologica Scan-
   dinavica (Eobeuhavn) Supplementum 61: 1965. 1l.l pp.
(44) KAHN, H. A. The Dorn study of smoking and mortality among U.S. veter-
   ans: Report on 8% years of observation. In: Haenssel, W., editor. Epi-
   demiological Approaches to the Study of Cancer and other Diseases.
   Bethesda, U.S. Public Health Service, National Cancer Institute Mono-
   graph No. 19, January 1966. Pp. l-125.

(45) KENBLEB, C. J. Cigarette smoke and clliastasis. Archives of Environmental
   Health (Chicago) 14 (2) : 371-372, February 1967.
  DALEAXN, T., RYL~NDEB, R. (Reply to Kensler) Cigarette smoke and cilia-
   stasis. Archives of Environmental Health (Chicago) 14(2) : 372-373,
   February 1967.

(46) KENSLEB, C. J., BATTISTA, 53. P. Chemical and physical factors a&ctlng
   mammalian ciliary activity. American Review of Respiratory Diseases
   (Baltimore) 93(3, pt. 2) : 93-102, March 1966.
(47) KILBURN. EC. H. Cilia and mucus transport as determinants of the response
   of lung to air pollutants. Archives of Environmental Health (Chicago)
   14(l) : 77-91, January 1967.

(48) KILBKI~N, K. H., SAIZANO, J. V., editors. Symposium on structure, function,
   and measurement of respiratory cilia. Duke University Medical Center.
   Durham, N.C., Feb. X+19,1965. American Review of Respiratory Diseases
    (Baltimore) 93 (No. 3, pt. 2) : 184 pp., March 1966.

(49) KauM~o=, R. A., CHEVAL~B, R. B., Ross, J. C. Cardiopulmonary function
   in young smokers. A comparison of pulmonary function measurements
   and some cardiopulmonary responses to exercise between a group of young
   smokers and a comparable group of nonsmokers. Annals of Internal
   Medicine (Philadelphia) 60(4) : 603-610, April 1964.

114


(50)

(51)



(52)



(53)



(54)



(55)

(56)

(57)

(5'8)

(59)

(GO)

(61)

(62)

(6.3)

(64)

(65)

(66)

(67)

KBUMHOL~, R A., CHEVALIEE, R. B., Ross, J. C. Changes in cardiopulmonary
functions related to abstinence from smoking. Studies in young cigarette
smokers at rest and exercfse at 3 and 6 weeks of abstinence. Annals of
Internal Medicine (Philadelphia) 62(2) : 197-267, February 1965.
KBUMHOEZ, R. A,, CH~XALIEB, R. B., Ross, J. C. A comparison of pulmonary
compliance in young smokers and nonsmokers. American Review of Res-
piratory Diseases (Baltimore) 92( 1) : 102-107, July 1965.
~BSON, R. K., BABMAN, 111. L. The familial occurrence of chronic obstruc
tfve pulmonary disease. Annals of Internal Medicine (Philadelphia) 63
(6) : 1001-1003, December 1965.

LUNDMAN, T. Smoking fn relation to coronary heart disease and lung fun+
tion in twins. A cotwin control study. Acta Medica Scandinavica (Stock-
holm) 130 (Supplement 455). 75 pp., 1966.
MASIN, F., MASIN, M. Frequencies of alveolar cells in concentrated sputum
specimens related to cytologic classes. Acta Cytologica (Baltimore) 10
(5) : 362-367, September-October 1966.
I~~C~~, J., CASSELL, E. J., WOLTER, D. W., MOUNTAIN, J. D., DIAMOND,
J. R., MOUNTAIN, I. M. Health and the urban environment. V. Air pollu-
tion and illness in a normal urban population. Archives of Environmental
Health (Chicago) 14 (1) : 173-133, January 1967.
Mr~xxa, J. M., SPBOULE, B. J. Acute effects of inhalation of cigarette smoke
on mechanical properties of the lungs. American Review of Respiratory
Diseases (Baltimore) 94(a) : 721-726, November 1966.
MOUZAKIS, 5. T. Personal communication. Athens, University of Greece,
May 17,1967.
PAYNE, M., K~LBBEBO, M. Respiratory symptoms, lung function, and smok-
ing habits in an adult population. American Journal of Public Health
and the Nation's Health (New York) 54(2) : 261-277, February 1964.
Pxrass, J. M., Fmuus, B. G., JR Smoking, pulmonary function and respfra-
tory symptoms in a college-age group. American Review of Respiratory
Diseases (Baltfmore) 95(5) : 774-732, May 1967.
Pxrxas, J. M, Fxaars, B. G., Ja Smoking and morbidity in a college-age
group. American Review of Respiratory Diseases (Baltimore) 95(5) :
783-789, May 1967.
Pxrrr, T. L., RYAN, 8. F., MITCHELL, R. `5. Cigarette smoking and the lungs.
Relation to postmortem evidence of emphysema, chronic bronchitis, and
black lung pigmentation. Ardhives of Environmental Health (Chicago)
14( 1) : 172177, January 1967.
RANKIN, J., Gmr, J. B. L., CHOSY, L. W. The influence of age and smoking
on pulmonary diffusing capacity in healthy subjects. Medfcina Thoracalfs
(Basel) 22(3) : 366-374,1965.

ROBBINS, m. T. Bronchial epithelium in smoking and nonsmoking college
students. Journal of the American College Health Association (Ithaca)
14 (4) : 265-266, April 1966.
Room% E. E., SPEEB, F. D. The ill effects of cigarette smoking in dogs.
International Surgery (Chicago) 46(6) : 52&530, December 1966.
SAW 3. I. Role of Pulmonary eurfactant in health and diseaqz Medical
Clbffcs of North America (Philadelphia) 51(2) : 391+r2, March 1967.
SLun+Cax~aa G. K., W~~rass, L. G., SICHPJL, H. 5. Chronic bronchitis fn
miners and norffniners : An epidemiological survey of a community fn the
gold-mining area in the Transvaal. British Journal of Industrfal Medicine
(London) 24(l) : l-12, January 1967.

`SLnrs-~f& G. K., W~LTESZS, L. G., .SICHEL, H. 19. Ventflatory function
in relation to mining experience and emoking in a random sample of

115


(68)

(69)

(70)



(71)

(73)

(73)

(74)

(75)

(76)

(77)

(78)

(79)

(80)

(81)

miners and nonminers in a Witwatersrand town. British Journal of
Industrial Medicine (London) 24(1) : 13-26, January 1967.
U.S. PUBLIO HE~LTE Snavrcz Smoking and Heal& Report of the Advlaorg
Committee to the Surgeon General of the Public Health Service. [Wa&
ington.] U.S. Department of Health, Education, and Welfare, pnrblic
Heanlth Service Publication No. 1103, 1964. 387 pp.
U.S. PWLIC mart SEBVICE National Center for Health `Statietics. Mar-
tality trends in the United States : lQ54-63. WaeZlington, U.S. Department
of Health, Education, and Welfare, Vital and Health Statistics Serim
20, No. 2, Public Health Service Publication No. lOQQ, June 1966. BP pp.
U.S. Pw~lc HEaLTH Sxavroe National Center for Health `Statistics. .&for-
tality. Vital Statistics of the United estates. Wash&ton, U.S. Department
of Health, Education, and Welfare. lQ5CMQ04.
U.S. PUBLIC HEXLTH S~VICE chronic Respiratory Diseases Control Pro-
gram. Beport of the Task Force on obronic Bronchitis and Emphysema.
National Tuberculc&s Association Bulletin (Mount Morris) %%(6) : l-23,
May 1987.
U.S. PWLIC HEALTH SEBVICE National Center for Health ~Statistlcs. Cig-
arette smoking and health characteristics, United States July lQ34-June
lQ85. Washington, U.S. Department of Health, Education, and Welfare,
Vital and Bealth Statistics Series 10, No. 34, Public Health Service
Publication No. 1000, May lQ67. Q4 pp.
WALKEB, T. R., Kmrxa, J. E. Ciliastatic components in the gas phaee of
cigarette smoke. Science (Washington) 153 (3741) : l248-l.250, September
9, 1988.
WEBB, W. R., COOK, W. A., Larmns, J. W., SHAW, R. R. Cigarette smoke
and surfactant. American Review of Respiratory Diseases (Baltimore)
95 (2) : 244-247, F&ma@ 1967.
WIOKER, A. J. Environmental and personal factor;s in lung cancer and
bronchitis mortality in Northern Ireland, lQQO-62. London, England.
Tobacco Reseamh Council, Research Paper 9,lQtM. 84 pp.
WINKELBTEIN, W., JR, KANTOB, S., DAVIS, E. W., m, C. S., Mosam,
W. E The relationship of air pollution and economic statue to total
mortality and selected reupiratory system mortality in men. I. Suspended
particulate% Arehives of Environmental Health (Chicago) 14(l) : 162-
171, January 1967.
WYNDEB, E. L., GOODMAN, D. A., HOFFMANN, D. Ciliatoxic componente in
cigarette smoke. II. Carboxylic acids and aldehydes. Cancer (Philadel-
phia) 18(4) : 505-509, April lQ66.
WTY~DIZB, E. L., GOODMAN, D. A., HOFFMANN, D. Cilia&x& components in
cigarette smoke. III. In vitro comparison of different smoke com~ponents.
Cancer (Philadelphia) 18 (12) : 165~1633, December 1966.
WYNDEB, E. L., -WEB, H. E., GOODMAN, D. A., HOFFMANN, D. A method for
determining ciliastatic componenbs in cigarette smoke. Cancer (Philadel-
phia) 16(Q) : 1222-1225, September 1963.
WYNDEE, E. L., LEMON, F. R., MANTEL., N. Epidemiology of persistent cough.
American Review of Respiratory Diseases (Baltimore) Ql(5) : 679700,
Nay lQQ5.
ZWI, S., GOLDMAN, H. I., Lrwm, A. Cigarette smoking and pulmonary func-
tion in healthy Young adults. American Review of Respiratory Disease8
(Baltimore) 89(l) : 73-81, January 1984.

116


SUPPLEMENTAL REFERENCES

~1. -OTT, 0. A., HOPICINB, W. A., VAN FLEIT, W. E., `ROBIIWOI$ J. 1. A new
  rpproach to pulmonary emphysema. Thorax (London) 8(2) : 11%132,
   19!S3.

~2. ADELMAN, J. U. A review and reappraisal of emphysema. Diseases of the
   Chest (Chicago) 51(2) : X6-161, February 1967.
53. ANDEBSON, A. E., JB, dscmr, A., BA TCEELDEB, T. Ii., FOBAKEI+ A. G. Exper-
   imental analysis in dogs of the ,relationshlp b&ween pulmonary elupw-
  eema, alveolitis, and hyperinflation. Thorax (London) 19(g) : 42Q-432,
   1964.

S4, ANDEBSON, D. 0. Observations on the clazwifleation and disixfibution of
  pulmonary emphyeema in Canada. Canadian Medical Association Jour-
   nal (Toropto) @( 14) : 709-716, @ct. 5,1663.
55. ANDEBSON. D. O., Fmuus, B. G., Ja. Community studiea of the health effects
   ef air polrlution-a critique. Canadian Journal of ~Public Health
   (Toronto) 57 (6) : 269-226, May 1266.

56. ANDEESON, D. O., ZICKMAXTEL, R., FEBBIs, B. Q., JR Response to a ~eepiTa-
   tory survey. Canadian Medical Association Journal (Toronto) 88 (12) :
   596-602, Mar. 23,1Q63.

~7. ANDEBBON, R. J. Epidemiologic studies of air pollution. Diseases of the
   Ohm (Chicago) 42(5) : 474-431, November 1962.
S& ANDOSCA, J. B. The incidence of smoking and the smoking habits in 5QQQ
   registrants for military service. Jourual of the American Geriatrics
   Society (Baltlmere) 14(Q) : 631-622, June 1988.
SQ. AUEIXBACEC, O., STOUT, A. P., HAMMOND, E. C., GAWINEEL, L. Emphysema
   and other pulmonary changes in smokers. Histologic evidence. Post-
   graduate Medicine (Minneapolis) 40(l) : 96-100, July 1986.
810. A-0, D. M., lS~~~~~, M. Bronchepulmonary effects of tobacco and
    related substances. I. Bronehwonstrlction and bronchodilatatlon : Influ-
   ence d lung denervation. Ar&ives of Entironmental Health (Ohicago)
   11(2) : 141-151, August 1985.

Sll. Avrano, D. Id., `SAXAXEK, M., FOLLX, L. E. Cardiopulmonary effects of
   tobacco and mated substances. I. The release of histamine during
   inhalation of cigarette smoke and anoxemia in the heart-lung and
   intact dog preparation. bchives of Environmental Health (Chicago)
   l2(6) : 705-7ll, June 1966.

512. BAETJEB, A. M., BATE@, L. M. EK&s of environmental temperature and
   vapor pressure on cilia-mucus clearance rates. In : Proceedimp of the
   International Congress on Occupational Health, Vienna, Austria, Sept.
   1%%,1966. pp. 675876.

513. BEECKMANS, J. M. The deposition of aerosols in the respiratory tract. II.
   `Deposition in cigarette smoking. Canadian Journal of Physiology and
   Pharmacology (Ottawa) 43(6) : 707-714, ~September 1~65.
514. BLMKBUKN, H., TAYLOIG H. L., PAELIN, R. W. KIHLBEBO, J. KEYS, A. Phys-
   ical activity of occupation and cigarette smoking. R&ationemlp to
   ventilatery function and respiratory symptoms. AMives of Environ-
   mental Health (Chicago) lO( 2) : 312-322, February 1985.
S15 BoArMAx, E. S., MABTIXV, H. B. Electron micmwcepy in pulmonary emphy-
   eema of rat&its. AWriCan Review of Respiratory Diseases (Baltimore)
   91W : X%265, F&XMTY lQQ6.

SIQ Born,, H. G. Experimental emphysema. Basis, review, and critique. The
   American Review of Respiratory Diseases (Baltimore) 92(l) : i-15,
   Jnly 1665.

117


817. BOBEN, H. G., Bnaw, J. F., C%EF%, R. F., Gnzyuowsur, S., NEWMAN, M. M,
    OBQAIVICK, A. B., Lssrxa, W. Treatment of drug-resietant tuberculosis:
   a statement by the Comm&tee on Therapy. American Review of Respire+
   tory Diseases (Baltimore) Q4( 1) : 125-l.27, July 1988.
S18. Boasx, H. G., DELAHANT, A; B., STEENKEN, W., Jr. Reaction of guinea
   pig lung to injected lung homogenates. Medlcina Thoraealis (Basel)
    22 (3) : 365-364,1965.
519. BONN, H. G., KOBY, R. C., SYNEB, J. C. The Veterans Administration army
   cooperative study of pulmonary function. II. The lung Volume and its
   subdivisions in norms1 men. American Journal of Medicine (New York)
    41(l) : Q6-114, July 1966.
S20. Boanx, H. G., -EL, V. E. Ventilatory, neural, and other faOtOrS influenc.
    ing the pulmonary microcirculation of the frog. Bibliotheca Anatomica
    ( Basel) 7 : 86-91,X%35.
521. BBINKYAN, G. L., Bm, D. L. The prognosis in chronic bronchitis. Journal
    of the American Medical Association (Chicago) 197 (1) : l-7, Jnls 4,lQ66.
522. Buxaxss, A. M., Buaoxss, 8. B. Chronic obstruetlve pulmonary emphysema.
   A review, with special reference to its relation to cigarette smoking.
   Rhode Island Medical Journal (Providence) 49(8) : 46U66.494, August
    lQ66.
523. BWNS, M. W., MAY, J. R. Haemophilus influensae precipitins in the serum
    of patients with chronic bronchial disorders. Lancet (London) l(7486) :
    354-358, Feb. l&1987.
524. Buaaows, B. Pulmonary diKusion and alveolar-capillary block. Medical
   Clinics of North America (Philadelphia) m(2) : 427-438, March 1987.
825. Bussows, B., Fzvzrc~xa, C. M., HEABD, B. E., JONES, N. L., W~~I.IFF, J. 8.
   The emphysematous and bronchial types of chronic airways obstruction.
   A cllnlcopathologlcal &dy of patients in London and Chicago. I&meet
    (London) l(7442) : M, Apr. 16,1Q66.
526. C!rzxarir~c~, N. S., Dowrzxo, H. F., CA~BTON, R. W., MCB~YDE, V. E. The role
    of acute lower respiratory infection in causing pulmonary insufllciency
    in bronchiectasis. Annals of Internal Medicine (Philadelphia) 66(3) :
    48Q4Q7, March 1967.
527. Chronic bronchitis and occupation. British Medical Journal (London)
    1(547Q) : 101-102, Jan 8,1Q66.
528. COHEIQ, B. M. Sympathomimetic amine aerosol administration in chronic
    obstructive ventilatory disease. A one year trial in patients abstain&
    from cigarettes and a matched group who continued to smoke. Medical
    Times (Manhasset ) Q4 (3) : 36&35Q, March X366.
529. Coorxw, J. R. T., HOLLAND, W. W. Social and environmental factors in
    respiratory disease. A preliminary report. Archives of Environmental
   Health (Chicago) 14(l) : 157-161, January, 1987.
530. COTES, J. E., ROIXSITEB, C. E., HIQQIN~, I. T. T., G-ON, J. C. Average
    normal values for the forced expiratory volume in white Caucasian
   males. British Medical Journal (London) l(54Q4) : 1016-1019, Apr. 2%
     1988.
531. CBO~N, E. C. A comparison of the mortality from bronchitis in Scotland
    and in England and Wales. British Medical Journal (London) l(6451) :
    163&163Q, June 26,1Q66.
532. CTJLLEN, J. H., KAY, H. L., KAEMMEELEN, J. T. Chronic ditruse pulmonary
    infiltration and airway obstruction. American Review of Respirators
    Diseases (Baltimore) 92 (5) : 775-780, November, lQ66.
833. DAMON, A. Negro-white diKerences in pulmonary function. (Vital capacity,
    timed vital capacity, and expiratory flow rate.) Human Biology (De-
    troit) 38(4) : 38Q-3Q3, December lQ66.

118


834. DxMa~tuus, G. A. The meteorology associated with New Orleans asthma.
   Archives of Environmental Health (Chicago) 11( 6) : 787-793, December
    1985.

835. DOBMANDY, T. L. Abnormal oxygen-dissociation curves (letter). Lancet
   (London) 2(74(n) : 80-81, July lO,I965.
,936. EDWAIW, G. Acute bronchitis-aetiology, diagnosis, and management.
   British Medical Journal (London) l(5493) : 963-966, Apr. 16,1966.
837. ENTEBLINE, P. E., LAINEABT, W. S. The relationship between coal mining
   and chronic nonspecific respiratory disease. American Journal of Public
   Health and the Nation's Health (New York) 57(3) : 484-495, March
    1967.

~38. The ethnic distribution of disease in the United States. Journal of Chronic
   Diseases (St. Louis) 20(3) : 115-118, March 1967.
839. FILLET, G. F. Emphysema and chronic bronchitis: Cllnjcal manifestations
   and their physiologic significance. Medical Clinics of North America
   (Philadelphia) 51(2) : 283-292, March 1967.
840. FLETCHEB, C. M. Some recent advances in the prevention and treatment of
   chronic bronchitis and related disorders. With special reference to the
   effects of cigarette smoking. Proceedings of the Royal Society of Medicine
   (London) 58 No. 11, pt. 1) : 918-928, November 1965.
541. FOBSTEB, R. E., ROUOHTON, J. W., CANDEB, IA, B~ECOE, W. A., Ruxuxnu, F.
   Apparent pulmonary diffusing capacity for CO at varying alveolar Oa ten-
   sions. Journal of Applied Physiology (Washington) ll(2) : 277-289,
   September 1957.

542. GERBER, P. An lnfeetfous deoxyribonucleic acid derived from vacuolating
   virus (SVm). Virology (New York) 16(l) : 96-97, 1962
s43. GLASSEB, M., GBEENBUBO, L., FIELD, F. Morbidity and mortality during an
   episode of high air pollution, New York City, Nov. 28-25, 1966. April
   1967. [Unpublished.] 92 pp.

S44. GOLDSMITH, J. R. Air pollution medical research. Science (Washington)
   154(3756) : 1588-1591, Dec. 281966.

545. GOLDSMITH, J. R. Epidemiology of bronchitis and emphysema. I. Factors
   influencing prevalence and a criterion for testing their interaction.
   Medicina Thoracalls (Basel) 22(l) : l-23,1983.
S4G. GOLDSMITH, J. R., GBEEZTBUE~, L., ALTBHOLLEB, A. P., SPICEB, W. 8.. JB.,
   CASSEL.L, E. J., LANDSBFZBQ, H. E. Air pollution and health. A statement
   by the Committee on air pollution. American Review of Respiratory Dis-
   eases (Baltimore) 93(2) : 302-812, February 1966.
547. GWIEN, H. L. Respiratory protection against particulates-problems solved
   and unsolved. American Industrial Hygiene Association Journal (De-
   troit) 26(3) : 263-211, MayJune 1965.
548. Game, I. Chronic bronchitis and occupation (letter). British Medical Jour-
   nal (London) l(5488) : 675-676, Mar. l.2, 1966.
549. HA~~TBOM, R. M., Srs.~oux, H. A., LANDAU, E. !I& Nashville air pollution
   study : VII. Mortality from cancer in felation to air pollution. U.S. Public
   Health Service, Division of Air Pollution, Contract No. SAph 69628 and
   Contract PH86-5&157. [Unpublished.] 17 pp.
s50. OTT, W. Y. Effect of ozone and cigarette smoke on lung function. Ar-
   chives of Environmental Health (Chicago) 10 (2) : 295-302, February
    1965.

S5I. -MMoND, E..C. Smoking in relation to mortality and morbidity. Findings
   in first 34 months of followup in a prospective study started in 1f+59.
   Journal of the National Cancer Institute (Washington) 32(s) : 1161-
   lx% May 1964.

271-804 O-674                           119


S52. HAMMOND, E. C. Some preliminary tlndlngs on physical complaints from
   a prospective study of 1,064,004 men and women. American Journal of
   Public Health and the Nation's Health (New York) 54(I) : 11-23, Janu.
   ary 1964.
553. HAX~MO~D, E. C., BEBOLU~D, L., KABLOW, R. Smoking habits and disease br
   Minnesota. Minnesota Medicine (St. Paul) 48(l) : 44-49, January 1965.
554. HAMMOND, E. C., FENT, L. S., Rnnn, D. C., WILSON, J. L. Smoking habits
   and health in Kansas and other central states, Journal of the Kansas
   Medical Society (Topeka) 65(12) : 586-590, December 1964.
555. HAMMOND, E. C., Gnasnn, L F. Smoking habits and disease in Ohio. OMo
   State Medical Journal (Columbus) 6l(2) : 134-137. February 1065.
S66. HAYMOHD, E. C., MUBPEY, R., BAEZB, S. Smoking, air polhtiqn and health
   in California. California Division of the American Cancer Society and
   the Statistical Research Section, Medical adlairs Department, American
   Cancer Society, 1984.22 pp.
967. HA&~YOND, E. C., Srsmrr, E. C. Smoking habits and disease in Illinois.
   Illinois Medical Journal (Chicago) l26(6) : 661-665, December 1964
868. HAMMOND, E. C., VAN GEXETH~~PSE~, T. H., Dmszna, J. B., SNEIXMX~, A. M.,
   HALLIQA~, W. Smoking habits and disease in New York State. New
   York State Journal of Medicine (New York) 65(20) : 2557-2561, Oct.
    16,1965.
559. HAMMOND, E. C., WILSON, 0. Smoking habits and disease in Missouri.
   Missouri Medicine (St. Louis) 62(2) : 109-112, 122, February 1965.
560. HA~IX&FT, R. G. Tokyo-Yokohama asthma. A review and some current
   concepts. California Medicine (San Francisco) 105(2) : 89-92, August
    1966.
S61. HEIMANN, H. Status of air pollution health research, 1966. Archives of
   Environmental Health (Chicago) 14 (3) : 48%503, March 1967.
S62. HFZ~NAXDEZ, J. A., ANDEB~~H, A. E., JB, HOLMES, W. L. FOG A. G.
   Pulmonary parenchymal defects in dogs following prolonged cigarette
   smoke exposure. American Review of Respiratory Diseases (Baltimore)
    93(l) : 7883, January 1966.
563. HIQMIVS, M., KJELBBEBO, M. Characteristics of smokers and nonsmokers in
    Tecumseh, Mich. II. The distribution of selected physical measurements
    and physiological variables and the prevalence of certain dlseasea in
   smokers and nonsmokers. (In preaa ) American Journal of Epidemiology
    (Baltimore) : 1067.
S64. HOL~AWAY, M. D., TWK, D. C. Capsulated haemophilus inflnensae and
   respiratory-tract disease. Lancet (London) l(7486) : 358-360, Feb. 18,
    lss?.
865. HOLLAND, W. W., I&m, D. D., SELTSEB, R., STONE, R W. Respiratory disease
   in England and the United States. Studies of comparative prevalence.
   Archives of Environmental Health (Chicago) lO(2) : 338-343, Febm-
    ary 1965.
586. HOLUND, W. W., STOX?E, R. W. Respiratory disorders in United States
   east coast telephone men. American Journal of Epidemiology (Balti-
    more) 82(l) : 92-101, July 1965.
567. HONG, C. S., GAXDI~IA, B., LOWELL, H. Ventilatory capacRy in a series of
   male adults and the effect of respiratory symptoms, productive cough,
   smoking habit and occupation. Medical Journal of Australia (Sydney)
    l(4) : 169-172, Jan. 281967.
568. KIKKAWA, Y., MOTOYAMA, E. K,, COOK, C. D. The ultrastructure of the
   lungs of lambs. The relation of osmiophllic inclusions and alveolar lin-
   ing layer to fetal maturation and experimentally produced respiratory


distress. American Journal of Pathology (New York) 47(5) : 87'7-889,
November 1965.

S69. KILBUBN, K. H. Mucociliary clearance from bullfrog (Rana catesbeiana)
   lung. U.S. Public Health Service, National Institute of Allergy and In-
   fectious Diseases. Research Grant Al 97617. [Unpublished.] 2l pp.
~76. K~uBI?, K. H. Pathogenesis and treatment of chronic bronchitis. Medical
   Times (Manhasset) 95(Z) : 161-176, February 1967.
~71. Korra, P. Opportunities in environmental and occupational pathology.
    Laboratory Investigation (New York) 13(6) : 619-623, June 1964.
~72. KOUBI~~KY, R., BBILLE, D., HA-, M. J. Etude statistique de la relation
   entre le tabac et la bronchite chronique. Bulletin de 1'Academie Nationale
   de Medecine (Paris) X%(17-18) : 318329, May 24, 1966.
~73. -ON, R. K., Frrxun~, P., Mvarra~, J. F. Systemic and pulmonary
   vascular effects of nicotine in anesthetized dogs. American Review of
   Respiratory Diseases (Baltimore) 91(4) : 556-564, April 1965.
~74. LEG- R. A. Recovery of culturable tobacco mosaic virus from sputum
   and thoracentesis fluids obtained from cigarette smokers with a history
   of pulmonary disease. American Review of Respiratory Diseases (Balti-
   more) 95(3) : 519-511, March 1967.

575. LEMON, F. R., W~L~EH, R. T. Death from respiratory system disease among
   Seventh-Day Adventist men. Journal of the American Medical Associa-
   tion (.Ohlcago) 198 (2) : 117-126, Oct. 10,1966.
576. Lxuo~rn~~naoxa, C., LxUcurrx~sxaoxa, R. Kumulative Wirkung von
   Zigarettenrauch-Inhalation und Influenza-Virus-Infektion bei der
   Entstehung von atypischen Proliferationen im Bronchialepithel von
   Mliusen. Oncologia (Basel) 19(l) : Sl-164,1965.
S77. LEWIS, C. E., KEBBY, G. R. An epidemic of polymer-fume fever. Journal of
   the American Medical Association (Chicago) 191(5) : 375-378, February
   1,1965.

578. LOWELL, F. C., ~NKLXN, W., MICHELSON, A. L., SCHILLEB, I. W. Chronic
   obstructive pulmonary emphysema : a disease of smokers. Annals of
   Internal Medicine (Philadelphia) 45(2) : 268-274, August 1956.
579. MCBAY, A. J. Carbon monoxide poisoning. New England Journal of
   Medicine (Boston) 272(5) : 252-253, February 4, 1965.
586. MCILL~EATH, F. J., COHEN, B. M. Ventilatory performance of American
   physicians. A pilot study. .&nerican Journal of the Medical Sciences
    (Philadelphia) 252 (1) : l-8, July 1966.

S81. MASS~BO, D., Barroars, L. Epidemiology of chronic bronchitis. Medical
   Times (Manhasset ) 95( 2) : 121-128, February 1967.
582. ME~AHED, G. E. The role of smoking in chronic bronchitis in Egyptians.
   Royal Egyptian Medical Association Journal (Cairo) 49(5/g) : 313-328,
    1966.

583. Mrr.rxa, W. F. Rehabilitation of patients with chronic obstructive lung
   disease. Medical Clinics of North America (Philadelphia) 51(2) : 34%
   361, March 1967.

S84. MITCHEIX,, R. S., RYAN, S. F., Pxrr~, T. L., FILLEY, G, F. The significance
   of morphologic chronic hyperplastic bronchitis. American Review of
   Respiratory Diseases (Baltimore) 93(5) : 720-729, May 1966.
S85. MITOHELL, R. S., VIN~NT, T. N., FILLXY, G. F. Cigarette smoking chronic
   bronchitis, and emphysema. Journal of the American Medical Associa-
   tion (Chicago) 188( 1) ; 132-138, Apr. 61964.
S86. MIT~HECL, R. S., WBB, N. C., Frrrxr. G. F. Chronic obstructive broncho-
    pulmonary disease. III. Factors influencing prognosis. American Review
   of Respiratory Diseases (Baltimore) 89(6) : 878-896, June 1964.

121


S87. MOFFAT, M. A. J., SUTH~ZLA~D, J. A. W. Persistence of viral antibodies hi
  patients with chronic bronchitis. British Medical Journal (London)
   l(5540) : 601-603, Mar. l&1967.

588. MOLHO, M., Baz, I.. Karxsnna, B. A baeis for differentiating between pri-
   mary emphysema and pulmonary obatructlon disease due to chronic
   bronchitis. Proceedings Tel-Hashomer Hospital (Tel-AY~Y) 5(2) : !,&35,
     June 1966.

989. MOBQAB, W. K. C. Chronic bronchitis. The "English disease." Postgraduate
   Medicine (Minneapolis) 41(2) : 190-194, February 1967.
890. Mwvrrc, Q. N., EVAXE, D. G. Metabolic and Immunologic activities op
   alveolar macrophages. Archives of Environmental Health (Chicago)
   14(l) : 92-96, January 196'7.

S91. NASH, E. S., BBISCOE, W. A., COX~XAND, A. The relationship between
   clinical and phyeiologlcal findings in chronic obstructive disease of the
   lungs. Medicina Thoracalis (Basel) 22: 305-327, 1965.
592. PAEN- J. L., A~~xxso~, D. O., K~N~Is, C. Cigarette smoking and respira-
   tory infections in a class of student nurses. New England Journal of
   Medicine (Boston) 274( 18) : 979-984, May 5,1966.
593. PeLzEB, A. M., THOMSON, M. L. Effect of age, sex, stature, and smoking
   habits on human airway conductance. Journal of Applied Physiology
   (Washington) 21(2) : 46w76, 1966.

S94. PEMBEBTON, J. Oecnpational lung disease (letter). British Medical Journal
   (London) l(5487) : 609, Mar. 41966.

595. Pna~trrr, S. Pulmonary surfactant: its depletion and regeneration in
   excised lungs. Baltimore, The Johns Hopkins School of Hygiene and
   Public Health, Department of Environmental Medicine. Supported by
   U.S. Public Health Service Grants HEOl!Z9, APO9298 (Division of Air
  Pollution, Bureau of St&e Services) and TG-HTS 5453; and by Navy
   Contract NR102-101. [Unpublished] 16 pp.

S96. Rem, D. D., AX?DI!ZXXON, D. O., Fnmue, B. G., FLerrc~im, C. M. An Anglo-
   American comparison of the prevalence of bronchitis, British Medical
   Journal (London) 2( 5423) : 1487-1491, Dec. 12, 1964.
597. REID, D. D., C~~~IELD, J., MABXUSH, R. E., SEIQEL, D., PEDEBBW, YL,
   HA~NS~EX, W. Studies of disease among migrants and native popula-
   lations in Great Britain, Norway, and the United States. III. Preva-
   lence of cardio-respiratory symptoms among migrants and native-born
   in the United States. In: Haensxel, W., editor, Epidemiological Ap-
   proaches to the Study of Cancer- and Other Diseases. Bethesda, U.S.
   Public Health Service, National Cancer Institute Monograph No. 19,
   January 1966. pp. 321-346.

S98. REIXANN, H. A. Acnte respiratory tract infections in the aged Geriatrics
   (Minneapolis) 22 (3) ; 169-167, March 1967.

S99, RENZIETTI, A.D. Prognosis in chronic obstructive pulmonary disease. Medi-
   cal Clinics of North America (Philadelphia) 51(2) : 363-371, March 1967.
5100. Rxnznrrr, A. D., JB, MCZCL~MENT, J. IL, &IT, B. D. The Veterans' Admin-
   istration cooperative study of pulmonary function. III. Mortality in
   relation to respiratory function in chronic obstructive pulmonary dis-
   ease. American Journal of Medicine (New York) 41(l) : 115-129,
    July 1966.

5101. Rroorrxnrx, M. L. Modern concepts in the management of emphyeema in
   the aged and infirm, Journal of the American Geriatrics Society (Bali+
    more) 14(5) : 497-504, May 1966.

5102. Saz~, 5. I. Role of pulmonary surfactant in health and diease. Medical
   Clinics of North America (Philadelphia) 51(2) : 391-402 March 1967.

122


S103. SALATA, J. R. Air pollution. Rocky Mountain Medical Journal (Denver)
    63 (10) : 47-51, October 1966.

Sl&t. SAMANEK, M., Avr~no, D. M., PIGSKIN, G. W. Bronchopulmonary et7ect.s
    of tobacco and related substances. III. Axon reflexes elicited from
    the visceral pleura. Archives of Environmental Health (Chicago) 11(2) :
    160-166, August 1965.

SIO5. SCHUMAN, L. M. Epidemiologic approaches and problems in the assess-
    ment of causal factors in chronic respiratory disease. Journal of the
    Kentucky Medical Association (Louisville) 64(4) : 311-316, April X)&l.
S106. SEnIOs, R. M., F~SHMAN, A. P. Disturbances of alveolar ventilation. Medi.
    cal Clinics of North America (Philadelphia) 51(2) : 403-425, March 19137.
~107. SHAaP, J. 5!. PAUL, O., LrpPss, M. H., McKux?, H., SAXTON, a. A., JB.
    PIYYalenCe of chronic bronchitis in an American male urban industrial
    population. American Review of Respiratory Diseases (Baltimore)
    91(4) : 510-519, April 1965.

S108. SIMONSSON, B. G. Clinical and physiological studies on chronic bronchitis.
    III. Bronchial renctivity to inhaled acetylcholine. Acta Allergologica
    (Kobenhavn) 20(5) : 325348,1965.

5109. Spross, W. 5.. JB Relation of air pollution to disease. Archives of Environ-
    mental Health (Chicago) 9(5) : 600-605, November 1964.
SllO. SPODNIK, M. J., CUSHMAN, G. D., Ksss, D. H., BL~IE, R. W., Sp~oss, W. S.,
    Ja Eifects of environment on respiratory function. Weekly studies on
    young male adults. Archives of Environmental Health (Chicago) 13(2) :
    243-254, August 1966.

Sill. STANEK, V., Fonoa, J., HEJL, Z., WIDIXSKY, J., CHABVAT, P., SANTIIU~EK,
    M., ZBJIC, F., VAVSIK, M. A contribution to the epidemiology of chronic
    bronchitis. Acta Medica Scandinavica (Stockholm) 179 (6) : 737-746,
     June 1966.

5112. STEDMAN, R. L., MILLEB, R. L. Alhylating activity of cigarette smoke con-
    densate. Chemistry and Industry (London) l(14) : 618-619, Apr. 15,
     1967.

5113. THUBLBECK, W. M., ANOUS, G. E. A distribution curve for chronic bron-
    chitis. Thorax (London) 19 : 436-442, September 1964.
8114. TOMASHEFSKI, J. F., Ps~rr, P. C. Pulmonary emphysema: Pathology and
    pathogenesis. Medical Clinics of North America (Philadelphia) 51(2) :
    269-281, March 1967.

S115. VISWANATHAN, R. Acute effects of cigarette smoking on pulmonary artery
    pressures. Indian Journal of Medical Research (Calcutta) 53 (5) : 421-
    427, May 1965.

S116. VISWANATHAN, R., MODY, R. K., PUSAD, 5. S., SWHA, 8. P. Bronchial
    asthma and chronic bronchitis. A pilot survey. Journal of the Indian
    Medical Association (Calcutta) 46(9) : 48O-t83, Nov. 1, 1965.
S117. WADDELL, J. A. Bronchitis and emphysema. British Journal of Clinical
    Practice (London) 20 (9) : 477-480, September 1966.
5118. WEBB, W. R., COOK, W. A. LAN~TJS, J. W., SHAW, & R. Cigarette smoke
    and surfactant. American Review of Respiratory Diseases (Baltimore)
    95 (2) : 244-247, February 1967.

S119. WEISS, W. Cigarette smoking and diffuse pulmonary fibrosis. A pi'ellm-
   inary report. Archives of Environmental Health (Chicago) 14(4) : 564-
    568, April 1967.

8120. W~LHELMSEN, L., T~BLIN, G. Tobacco smoking in 50.year-old men. I. ReS-
   piratory symptoms and ventilator-y function tests. Scandinavian Journal
    of Respiratory Diseases (Kobenhavn ) 47 (2) : 121-1300.1966.

123


SE!l. WYNDEB, E. L, KdUFkfAIV, P. L., LsMxa, B. L A ShOrt-tellll fO~OWU~
    study on ex-cigarette smokers. With special emphasis on persistent
   cough and weight gain. (In press.) American Review of aspiratory
    Diseases (Baltimore) : February 1967.
5122. WYNDER, E. L., Lmfox, F. B., MANTEL, N. La epidemiologia de Ia tos per-
    sistente. Folia Clinica International (Barcelona) 15(Z) : 79--f% Febra-
    ary 1955; (3) : 123-130, March 1955 ; (4) : 901~!2lO, April 1965.
5123. 5smu3, L. D., HOBTOIP, R. J. M., LANDAU, E. The Nashville air pohu-
    tlon study: V. Mortality from diseases of the respiratory system in
    relation to air pollution. U.S. Public Health Service, Division of &
    Pollution. Contract No. SAph 69629 and PH 86588665-157. Presented
    before the Epidemiology Section of the American Public Health Asso-
    ciation at the Qlst Annual Meeting in Kansas City, MO., NOV. 12, 19139.
    [Unpublished.] 19 pp.
5124. ~EIDBEBO, L. D., PRIIDLE, R. A., IARDAU, E. The Nashville air pollution
    study. III. Morbidity in relation to air pollution. American Jonmal
    of Public Health and the Nation's Health (New York) 54 (1) : 8697,
    January 1954.


CHAPTER 3

Smoking and Cancer

CONTENTS

General Chemical and Experimental Data on Carcinogenesis
and Tobacco Smoke ________-_ - __________ - ____ -- _______
In Vi.t~o Cellular Changes by Tobacco Smoke ______ _ _ __ _
In V&o Tumor Formation by Tobacco Smoke- _ _ _ _ _ - _ _ -
Tumor-Promoting Agents in Tobacco Products----------
LungCancer_------------------------------------------
MortalityData_------------------------------------
Histopathology of Lung Tumors--- _ _ _ _ _ _ ____ ______ ___
Experimental Pulmonary Carcinogenesis- - _ _ _ _ _ _ _ _ _ _ _ _ _
Additional Evidence Concerning Experimental Carcino-
  genesis-__----------------------------------------
Cancer of the Buccal Cavity and Pharynx (Lip, Mouth,
Throat) _____________ - ______ - _________________________
CanceroftheLarynx__-_--------------------------------
Cancer of theEsophagus ____ ---_-__-_-_--___-__- _______ -_
Cancer of the Urinary Bladder------ _____________ -- _______
CanceroftheStomach____-_-----------------------------
Cancer of the Pancreas _________ - ____ -_-__- ____ --___- ____
Cited References ________________ -_- ____ -- _____________ --
Supplemental References------------------- ____ -- ______ -_

127
129
129
130
131
131
140
144

144

145
148
149
153
157
158
161
167

125


GENERAL CHEMICAL AND EXPERIMENTAL DATA ON
    CARCINOGENESIS AND TOBACCO SMOKE
polynuclear Aromatic Bydromrbons
As criteria for the presence of polynuclear aromatic hydrocarbons in
tobacco smoke, the list of J. W. Cook (m) has been widely accepted
by tobacco chemists.
The Surgeon General's 1964 Report and Cook's paper are in agree-
ment with respect to the presence of benzo(a)pyrene (3 : 4benzopy-
rene) , dibenz (a,h) -anthracene (12 : 5,6-dibenzanthrac) : benzo(c)
phenanthrene  (3 : 4benzophenanthrene),  and dibenzo ( a,i) pyrene
(3,4 : 9,10-dibenzopyrene) , all having carcinogenic activity.
Cook considers, furthermore, as identified : Benz (a) anthracene (1,2-
benzanthracene) marginal carcinogenic activity; chrysene, benzo(e)
pyrene (1,2benzopyrene), questionable carcinogenic activity ; benzo
(g,h,i) perylene (1,12benzoperylene) )2 benzo( b) fluoranthene (3,4-
benzofluoranthene) carcinogenic (69,106)) and benzo (j) fluoranthene
(10,ll~benzofluoranthene) carcinogenic (206).
Indeno (l&3-cd) pyrene (2,3-phenylenepyrene) has since been iso-
lated from tobacco smoke (@). This polynuclear aromatic hydrocar-
bon was found to be carcinogenic (44,59). The following carcinogens,
or questionable carcinogens, were isolated by Kiryu and Kuratsune
(55) in the smoke of cigarettes smoked by human volunteers: benz
(a) anthracene, chrysene, benzo (a) pyrene, benzo (e) pyrene, benzo (b)
fluoranthene and benzo (k) fluoranthene. The carcinogenic polynuclear
aromatic hydrocarbons are regarded as the major initiating car-
cinogens in tobacco smoke.
N-Eeterocyclic Aromatic Hydrocarbons
The Surgeon General's 1964 Report lists as carcinogenic compounds
three N-heterocyclics, dibenz ( a, j ) acridine, dibenz (a,h) acridine and 7
H-dibenzo- (c,g) carbazole. An independent investigation has con-
firmed the presence of the first named compound in cigarette smoke
WV *
N-Nitrosa7nims
N-nitrosamines are among the most powerful known animal car-
cinogens. Since tobacco smoke contains secondary amines (67, 71)
`Dibenzo (a,h)anthracene in the Surgeon General's lQ@ Report should be
replaced by dibenz (a,h ) anthracene (Ed).
* Benzo(g,h,i)perylene was not tested for carcinogenicity until 1966 and then
was found to & inactive (4).


and most tobaccos, certainly Burley and Maryland varieties, contain
nitrates (64)) tobacco smoke can be considered as a potential environ-
ment for the formation of N-nitrosamines. The major nitrates in
tobacco are alkaline nitrates.

Neurath, et al., isolated three aliphatic N-nitrosamines from the
smoke of a cigarette rich in volatile basic components and high in
nitrate content. One of them tentatively has been identified as methy-
n-butyl-nitrosamine (73).

When the particulate matter, "tar," was collected from cigarettes
not enriched with basic components or when the smoke particulate
matter was collected without aging and not in cold traps, N-nitros-
amines could not be isolated from cigarette smoke (79). Since the
only other publication concerned with the isolation of nitrosamines
in cigarette smoke was based on cold trap collection of "tar," the
positive finding of three N-nitrosamines appears questionable (86).

In summary, tobacco smoke can be regarded as a potential environ-
ment for the formation of N-nitrosamines. However, additional infor-
mation is needed to substantiate their presence in tobacco smoke.
Pozonium 910

Several investigators (33,35,50,76,99,93,1&?) have found trace
amounts of Po210 in tobacco leaf and cigarette smoke. The concentra-
tion of Po210 in lung tissue is relatively high (33, 67) as compared to
other body tissues and is higher in smokers than in nonsmokers (33,
48 64 66).

Lung tumors have been induced experimentally by intratracheal
implantation of various radioactive substances. These radioactive sub-
stances must, however, be present in the respiratory environment above
a certain threshold level and must be in contact with the target organ
long enough rto be effective (68,77,88,107). Because Poz'O emits alpha
particles, it has been implicated as a lung cancer initiator (@,68,76,
77). More research is needed before definitive conclusions can be made.
Until such time, however, PO *lo should be considered as a potential
tumor initiator in tobacco smoke.

Selenium

Selenium has been mentioned as possibly being important in the
pathogenesis of human lung cancer (100). Preliminary reports suggest
that selenium may be present in some cigarette papers. Because earlier
reports (17,34,97) indicated the ingestion of selenium caused cancer
of the liver in mice, a recent investigation (101) by the National Can-
cer Institute was conduoted, with negative results. So far the earlier
reports of the carcinogenicity of selenium have not been substantiated.
Additional information is needed on the possible carcinogenicity of
selenium and its presence in cigarette smoke before selenium can be
indicted as an agent in human cancer.

128


phenols

Tobacco smoke contains a large number of phenols (107). Several
of `them are known to be tumor promoting agents when applied in
high concentrations to mouse skin previously treated with a tumor
initiator (14).

IN VITRO CELLWLAR CHANGES BY TOBACCO SMOKE

Lasnitzki (60) extended her studies with tobacco smoke condensate
on cultured human fetal lung tissue to include a "highly purified
fraction of hydrocarbons" isolated from cigarette smoke condensate.
In 33 out of 50 treated lung tissue explants, the epithelium of the
bronchi was hyperplastic and sometimes showed squamous changes.
These changes were not observed wit.h the untreated controls.
Mthough a hydrocarbon-free fraction was weakly active by producing
some squamous metaplasia in these explants, these tissue culture tests
point strongly to carcinogenic hydrocarbons as the active group in
the smoke. The findings with purified carcinogenic hydrocarbons in
organ culture (91) support the finding that polynuclear aromatic
hydrocarbons are one group of active smoke constituents. Carcinogenic
hydrocarbons are also the only group of chemical components that
have been demonstrated in vitro to induce malignant conversion of
single cells (7,13).

In summary, tobacco smoke has been demonstrated in vitro to induce
pathological changes in tissue explants. Although such changes may
be induced by different smoke constituents, as yet the carcinogenic
hydrocarbons are the only agents identified in tobacco smoke which
have been shown to induce malignant changes in" tissue cultures.

IN Vrvo TUXOR FORMATION BY TOBACCO SMOKE

Passive inhalation experiments with tobacco smoke have not yet
led to fully established squamous carcinoma in mice (109). This
method of application has resulted only in papillomatous growth in
the tracheobronchial mucosa of a few hamsters. None of the tumors,
however, was found to be invasive (30,121). It appears that passive
inhalation may not lead to the induction of scpmmous cell bronchogenic
cancer in experimental animals. This conclusion can also be applied
to passive inhalation studies in which t.he animals are infected by a
virus before long-term smoke exposure (62, 110). The pathological
changes seen in the mice were reversible whether or not the animals
lvere previously infected with a virus. The hyperplasia and metaplasia
seen in mice and rats after passive inhalation appears, at least in part,
to be secondary to viral or bacterial infection that is enhanced by
exposure to tobacco smoke. The relatively negative findings with pas-

129


sive inhalation experiments probably relate to the relatively small
amounts of smoke aerosols that bypass the nasal passages. The defen-
sive nature of the upper respiratory tract against airborne irritanti
has to be fully appreciated in the evaluation of any passive inhalation
study.
Active inhalation studies with tracheostomized dogs, as carried out
by Rockey, (79,230) and Auerbach ($) , suggest that this approach
may lead to the induction of bronchogenic carcinoma. The change in
the bronchial epithelium after 1 year of active smoking indicates
early pathological changes t.hat may, upon continued smoke exposure,
lead to tumors in the bronchi.

So far, neither passive nor active inhalation studies have contributed
to our knowledge about the nature of the tobacco smoke carcinogens,
Studies with the particulate matter, tar, of cigarette, pipe, and cigar
smoke, however, have clearly demonstrated that at &he site of applica-
tion tumors can be induced. Tumors have been induced on the skin
of mice and rabbits, the ears of rabbits, the subcutaneous tissue and
hilum of rats and the cervices of mice (.9,21,9& 92, M, 46,.& 61,74,
82?,8t9,84,107,108).

Only relatively few investigators have been concerned with the
nature of chemical carcinogens in tobacco smoke (47, 84, 107). Al-
though t,he acidic and nicotine-free basic portions of tobacco tar had
been found to have weak tumorigenic activity, the only fraction shown
to have induced significant numbers of tumors is fraction B of the
neutral portion (2 percent of the whole condensate) (I#`). This B
fraction was further fractionated into three subfractions from which
only B, was shown to have tumorigenic activity (47). The B, frac-
tion equals 0.6 percent of the tar and combines all aromatic hydro-
carbons with three to seven rings including the carcinogenic ones.
This can be considered as evidence that in in zri~u studies, the poly-
nuclear aromatic hydrocarbons are the major carcinogens in tobacco
smoke. Although these compounds alone can account for only a small
portion of the tumorigenic activity of tobacco tar, they are, neverthe-
less, the only identified carcinogens and tumor initiators in tobacco
smoke shown by experimentation to be biologically active. Their
tumorigenic effect is enhanced by the presence of tumor-promoting
agents in &he smoke.

TUMOR -I?FUXK~~NG AGENTS IN TOBACCO PRODUCIS

In the experimental setting, the tumorigenicity of tobacco smoke
condensate cannot be solely explained by the presence of known car-
cinogens. In assays on mouse skin and rat subcutaneous tissue, the
known carcinogens must be enhanced by other components such IIQ:
tumor-promoting agents. In fact, it has been demonst.rated that to-

130


bacco extract and tobacco smoke condensate can act as promoters to
mouse skin previously treated with tumor-initiating carcinogenic
1,olycyclic aromatic hydrocarbons (10, 19, 96, 107). Although some
tumor-promoting activity of tobacco "tar" can be explained by some
phenols and carboxylic acids, additional tumor promoters in tobacco
products remain to be isolated and identified.

It is important, however, that a significant decrease of the poly-
nuclear aromatic hydrocarbons in tobacco "tar" leads to a significant
decrease of t.he overall activity of the %ar" on mouse skin (9,&?, 168,
109).
In summary, experimental studies have demonstrated that the par-
ticulate matter of tobacco smoke, "tar:' is tumorigenic. Some poly-
nuclear aromatic hydrocarbon-carcinogens have been identified as con-
tributing significantly to the overall tumorigenic activity of tobacco
smoke condensates in the experimental setting.

LUNG CANCER

MORTALITY DATA '

The annual number of deaths in the United States from cancer of
the lung (International Classification of Diseases, Codes 162,163) rose
from 18,313 deaths in 1950 to 45,338 in 1964 ($4). In this 15-year
period, deaths from lung cancer totaled 467,442. During this same time.
period the death rate for cancer of the lung almost doubled, a rise
from 12.2 deaths per 100,000 population in 1950 to 24 deaths per
100,000 population in 1964. (The corresponding age-adjusted rate has
also nearly doubled, therefore the increase in the death rate cannot be
attributed to the changing age composition of the population.) The
lung cancer mortality in the male population increased from 19.9
deaths per 100,000 population in 1950 to 41.4 in 1964, while in the
female population the deaths increased from 4.5 to 7.1 per 100,000
population over the same time period.
The mortality experience of the individual male cohorts during
1949-64 (fig. 1) shows that at any given age the risk of dying from
lung cancer was almost always higher for the more recently born
cohort. Within each cohort, the death rate for lung cancer increased
steadily to the end of the life span.

Figure 2 shows the death rate for women by cohort groups and age
at death. One can see the increasing death rate slope for each more
recently-born cohort, starting with cohort F-those women who were
26-30 years old in 1930. This corresponds to the time when smoking
became increasingly popular among women.

`AU death rates throughout this chapter are per 100,000 population unless
otherwise indicated.

131


    / fir,

ID
0.9 -

a* -

0.4 -
03 //,     ,     ,     ,     ,     ,
90    9* 90 99     40    49     90     99    90

A ..-
0.9
0.9

                          04
I     I      I      I      I      a3
99     10    79     .O    99."4
                    a.&.

FIWBE l.-Qancer of the lung among men, by birth cohort and age at death;
1@40,1964,1969, and 1964.


Fmuu Z.-Cancer of the lung among women, by birth cohort and age at death :
1!349,1954,1959, and 1984.


In the female population the greatest percentage increase-(116 per-
cent) over the 15-year period, 1949-64, occurred in the 35-44 year age
group. The next highest percentage increase was noted in the age
group 45-54 years. The death rate from lung cancer among women,
25 years and over, rose steadily with advance in age for each year
during 195&64, and the cohort experience shows that these death rates
continued to increase for each cohort to the end of the life span
Hammond's (4.0) prospective study provides extensive information
about the lung cancer mortality experience of both men and women in
relation to cigarette-smoking history as presented by mortality ratio *
and by death rates per 100,000 person-years. (Table 1).

TABLE 1 .-Lung cancer mortality ra&os andoh% rates * of amokem by
         sex am! specijfc age fpwup8

Mortality ratios- _ _- _____ _ __ ___    2. 17     7. 84    1 1.76     11.69
Death rates _____ ________-___-_  * (7)15  2 (12)87  f (17)30  ' (~3)262

lC.om
*Num E t
    tedfmmap .tablel9.
    ninpsrPmt eawiudi&ed&hratetorncm!mokm.
SOTJBIX: Hammond. E. C. [tablea 24 and 2J epp. table 19 (/o)].

Tables 2 and 3 below show the relationships of number of cigarettes
smoked per day, degree of inhalation, and age smoking began, to
lung cancer mortality ratios and death rates for males and females,
respectively. Generally, mortality ratios and death rates increase with
increasing amount of cigarettes smoked and degree of inhalation, and
with a longer lifetime history of smoking. Table 3 shows the relatively
lower lung cancer mortality among women as contrasted to men, but
reveals, for the most part, the same relationship to amount smoked,
degree of inhalation, and age when smoking began.
Table 4 illustrates the fact that cessation of cigarette smoking is
associated with a decline in lung cancer death rates.

o The mortal&y ratio is the ratio of the death rate of smokers to that af non-
smokers-the mortality mtb of nonsmokers always being one, by definition.

134


TABLE 2.-Lung cancer (men). Number of o?tmS, and &ge-stQndard&d
death rat&S and mdtiy rash, by current number of c&are&s
smoked per day, Mee of inhalahn, and age began smoking, by
age at start of study 1

-

c~ent numbex of cigarettea a day:
  * t(le _____ _____--__--__--__--____
  10 to Ill ________-____-___-__------
  20 to 39 __________________________
40 plus--. _ - _---_---_----_-------
Degree Of fllh8htiOll:
None or S&ht _____--__--________
  M&rate~-~ _ ______ _ ___ __ ___ _.___
  D-p- _ _____ ______________ _ __ _ ___
*4ge began elgaretta Qnou
  25 or older _______________________
  20 to 24 ____________________------
  15 to 19 ____________-___-___------
  Laps than 16 __--___-_--___---_---
Never smoked regularly ___-___--__--

Q
16
la8
!&I

1Q
114
55

5
a1
112
a5
11

-

Current numbsr of c@ratted B day:
  1 to Q _____________--___-_-------- -_---_-.
  10 to 19 -_--__--__---_------------ __-----.
  20 to a9 -_--__--__---_------------ _------.
40 plus _______-________-___------ __-----.
Degrea of inhalation:
None or slight ___________________ _______.
  Mien--.-------.--------.---- _______.
Deep. _ __ _ _ _ ___ _ _______-___-___-- ----__-.
Age began cigarette 8moHng:
  2.5 or older _______________________ _______.
  20 to m -___ ._--___-__-_- _ -------- -_ --__-.
  15 to 18 ---__-__-_--__---_-------- ------ -.
  Less than 15 _____________________ _______.

Hum-
berof
k&ha

12
67
2ls
50

a7
1Tf
72

12
72
175
s7
27

19    11
I I   2.5

E% "2
ieaths
--

w     m
a2     90
881    15Q
a2    201

l!20    102
all ls8
141    175


20     a9
110    118
816    155
1Ol    188
49     12

.-__----   4.60
.---_--_   7.48
._--_--_   la 14
._-__- --   16.61

I 1    I I I I
0.17 ____-__   a.63 _---_--_ haa
aw -_--___   a77 ________ 9.62
9.87 -_-----   18.82 ______-_ 17.M
7.57  __----_ ._  17.47 ______-_ 29.04

4.7b ____--_ .-  la&l --------   7.65
a4a _______ ,_  11.72 ______-_  1.5.88
0.00 ------_   taDa 1 I
              ______-_  26.24

2.77 __--_._   aal ______-_ aa6
5.89 -_---_- ,-  11.11 _-__---- 1211
8.71  ___--_ _   13.05 ---_---_ lQ.57
12.80 ___--_. ._  15.81 ---_--_- 16.76

        -

._---- --   a42
.; ------   11.44
._------   14. a1

_ - - _ _ _ _ -   3.21
--__--__   9.72
_ _ - _ _ _ - -   12 81
- _ _ - _ _ _ _   15.10

1 Mortality ratioa are based on de&h ratea carried out tn 1 more shnlticant llgum thanshown.
Sonam Hammond. E. C. [table 20 (&I)].

271-394 ~7-10

185


TABLE 3.-Lung cancer ( women). Number of o2uthq age-standardiz,?d
death ratea, and mortality ratios, by type of .smoking (lijetiime h&tory),
current number of cigar& smoked per day, degree of inhalation, and
age began smoking, by age at start of study l

Type of smokfng (lifetime bI6tor~)

Never smoked regularly. ____ ____ __ __ ____.
HlstoryofcigarettesmoHng~..... _____ ___

Current mmber of dgarettes a day:
  ltolQ--------..-.......~-------------
  ZOpltEL.. ~~~~~~-~_~~~~~~~~ _ _--___ ____
Degree of inhalation:
  None or dIgbte ____ ._ ____ _____ _____ ___
Modernteordesp __-----__ _ ___________
Age began SmoHng:

Iorolder _______________ _ _________ ___
Lessthan _______------ _ -________ ___

Never smoked regularly _____- ---_ _________
HIstory of cigarette smoking __________ _ ___

cnrrent number of cigareta B dny :
  1tolQ _____-________ _ ________ _ ________
  !mPlaa ---------_____.__ _._-__ ________
Degree of inhalatbn:
Noneordfgbt ______________ _ _______ __
Moderateor deep ___________ _ ______ ___
43 bagea -ohsI:

loroldsr.---..-.-------------------.
Les9tbanl~_~~~~___~_________ _ ______

                              -
Age 40-64      Age m-74     All sges. 40-74

1 Mortality ratloe are based on death ratea carrkd out to 1 more &nfficant &we than shown.
Sowx: Hammond, E. C. [table 23 (@)I.


TABLE 4.-Lung cancer (men). Age-standurdized d&h rates and mor-
t&ity ratios for ex-cigarette smokers wG!h a history of cigarette smoking
only, by jormer number of cigareti smoked per day, and years since
last cigarette smoking. Death rates for current cigarette smoker8 with
a history of cigarette smoking on4y. Men who never smoked regularly
are shown for comparison. Men ageo? 60-69.

U,&,r 1 yMU _-______________
l~lyealti ___------________-
5 tlJ 9 years -_--_______-___-_-
10p1usyem ---------_-------

   Total ex-smokem. _____
current clmrettesmokers~~- 22.808
Never smoked regal~~ly.-~~-

1 computed from source.
SOURCE: Hammond, E. C. [table 21 (JO)].

The Dorn study (49) of U.S. veterans provides additional informa-
tion on the relationship of dosage to mortality ratios and d&h rates
for males who smoked cigarettes only (table 5).

TABLES .-Lung cancer nwrtul~y ratios and d&z+% rates for US. veterana
       by age, type, and amount of smoking
      I            Nnmber of cfgmttea~dey

0        l-9       10-33      21-39

Current cigarette smokers
only:

Age 4s to 54 __-_----___--_ __---- __------ _----- -___--_-
Agesstoo4 __-_-__-____-- 10 I.00 70
Agetlsto74. -____________ a0 1.00 133
A3rraphu -____----__--__ 46
To&l. _ __________ ______

Ex-clgerette smokers only ____ ______ ________ ______

'DR,De&hrate;MR,Morhlitgmtio.
SOOBQC: U.H. vetamns study [app. table A (#I)].

rO+

DR MR
I

-___--   23.93
____-_   8.34

187


The mortality ratios of the Dorn (~$9) study can be compared with
those of the Canadian vebrans study, in table 6 :

TABLE &-Lung cancer mortd&!~ ratios for Canadian veteran by age,
        type, and amount of smoking

Current cigarette smokers only:
  Age30b49 ______________________ 1.00
  Age5Oto69 __________ - ___________   1.00
  Age 7Oplut3--- ____________________   1. 00
   Total ___________________________   1. 00

1-Q     10-20     21+

2. 47    4.15    4.08
10. 71   26.92   26. 33
12. 15    9. 43   24.53
la 00   16.41   17.31

Ex-cigarette smokers only total __________           0. 06

Bower: Camdlsn Pamionm study I(S), Table 8.1 and 8.21.

From the data shown in table 2 mortality ratios of 17.47 and 29.84
may bbe noted for smokers of 40+ cigarettes per day, age 55-69 and
70-84, respectively. The Dorn (-69) study (see table 5) similarly shows
mortality ratios of 33.80 and 23.20 for smokers of 40+ cigarettes per
day, age 55-64 and 65-74, resp&ively. The Canadian study (see table
6) shows mortality ratios of 26.83 and 24.53 for smokers 50-69 and 70
years of age and older respectively who smoked over 20 cigarettss per
day. There is rather close agreement among the three large prospec-
tive studies for the general range of mortality ratios observed in heavy
smokers. From the data supplied by the Doll and Hill survey of
British physicians (98, f29) a mortality ratio of 31.86 can be calcu-
lated for all smokers of more than 25 cigarettes per day, as com-
pared to a mortality ratio of approximately 8, for smokers of 1-14
cigarettes per day (sea table 8).

There is relatively little risk of lung cancer associated with pipe or
cigar smoking, probably ,&cause smoke from these sources is rarely
inhaled. "Mixed smokers," i.e., smokers of cigarettes, pipes, and/or
cigars, have less risk than do smokers of cigarettes only, also suggest-
ing that they may smoke fewer cigarettes or inhale less tobacco smoke
than do smokers of cigarettes only (see tables 7 and 8).

138


TABLE 7.-Lung cancer mod&y ratios by type and amount smokea?









~~~iwx: U.S. veterans study [app. table A W)l.

TABLE 8.- Lung cancer death rater by type of smoker and amount smoked









~vsce: Study of British phyzddmu [tables 22 and 24 (ts)l.

TABLE 9.- Lung cancer death raks for ez-smokers of c&are&a by
        length of time stopped smoking









somcs: Study of British Physicfans [table 25 C?41.
The preceding studies show appreciably lower mortality ratios and
death rates from lung canCer with the cessation of cigarette smoking
(see tables 4,5,6,7,8,9). This lower risk is evident irrespective of the
quantity of cigarettes formerly smoked.
The Doll and Hill study (a) of British physicians is of particular
interest in respect to ex-smokers. Over the M-year period of the study
(1951-61) 29 percent of the smokers of cigarettes only, had signifi-
cantly decreased (one-half pack cigarettes or more) their smoking (in-
cluding those who stopped) and 5 percent had switched to pipes
and/or cigars.
While the overall lung cancer mortality of men over age 25 in
England and Wales had increased 22 percent over this lo-year period,
that for the physician group decreased 7 percent. Since the total
physician group is involved in these figures, we can compare this
population group to the entire population of England and Wales
where there was no general decrease in amount of smoking. This can
be thought of as a controlled cessation experiment and the beneficial


effects of stopping or decreasing the amount of smoking become quih
evident.

Wicken (A&?), in a retrospective study of lung cancer mortality in
Northern Ireland during the period 1960-62, reported the following
results (Table 10) :

TABLE IO.-Lung cancer mortality ratios a?w! de&h rate.9; by sea?, age 86
and over, by type and amount of smoking, N&m Irelund, 1860-M

NOD
smokers  "s:z


       l-10   ll-!a !a+
     ---

Male:

  Mortality ratios- _ _ __ _ __
  Death ratea ____________
Female:
  Mortality rat&- _ __ ___ _
  Death ratea ____________

1.00  4.83 9.33 21.2
18    87 168 383


1.00  2 27 6. 72 19. 0
11    25 74 210

~OUBCE: Wiekeu, A. J. [(Icn), Table 17.

I

--

5. 22    2. 27
94     41

-__-----------__
-_----------we__

Wicken also analyzed the proportion of lung cancer deaths which
would have occurred if the lung cancer mortality rates of the least
susceptible groups had been applied to the whole population of North-
ern Ireland, and found that males would have had only 18 percent of
the lung cancer mortality if none smoked and that if they lived in
truly rural areas they would have only 10 percent of the mdrtality.
Thus, the difference percent-may be attributable to the urban or
suburban residence factor, possibly air pollution. If no females
smoked, they would have had only 65 percent of the total female lung
cancer mortality, and 53 percent if they lived in truly rural areas.
Thus, for females, the difference of 12 percentage points might be
attributed to the urban environment. The magnitude of these differ-
ences depends on the prevalence of lung cancer in the various sub-
groups of the particular population studied.

HISTOPATHOLOGY OF LUNG TUHO~

Classification of lung cancer by histologic type was discussed in the
Surgeon General's 1964 Report with the conclusion that the squamous,
undifferentiated, and oat-cell carcinomas were far more frequently
found in smokers than in nonsmokers, while adenocarcinoma was rela-
tively more frequent in nonsmokers, especially women. Changes in the
bronchial mucosa resulting from the inhalation of cigarette smoke in-
cluded loss of cilia, basal cell hyperplasia, and the appearance of
atypical cells with irregular hyperchromatic nuclei. These changes, it
was concluded, were related to the premalignant process of the de-

140


velopment of invasive carcinoma. Auerbach (6) has more recently
reported on a study of the pathology of the trabheobronchia] trees of
339 men who died from causes other than lung cancer and of 63 men
who died from lung cancer. Up to 55 cross-sections of the tracheo-
bronchial tissue were studied in each case. The 339 non-lung cancer
cases included 65 men who had never smoked cigarettes and 274 men
jvho had smoked in various amount. Figure 3 shows that only 1.3
percent of the slides from those who never smoked regularly have
60 percent or more atypical cells, whereas 76 percent of the slides of
those smoking more than two packs a day had 60 percent or more
atypical cells. (See figs. 3 and 4).

r  PERCENT OF SLIDES WITH MSJONS
  SHOWING 603 OR MORE ATYPICAL CELLS

76.8

t                                  34.9

/.4, 14.7, `I
.,,3,

I

Novrr Smoked   <l/2 Pock l/2-l PoeIt l-2 Pocks 2t foe IO

92.1

e-m I
     LUlS

I

R*qulorly

4 clay    A cloy    P ooy   A Day       CDllCOr

Fx~uu~ 3.-Percent of elides with lesions showing 60 percent or more atspical
                        cells

Source : Auerbach, O., et aI. [Table l(5), updated 1967J

Auerbach (4) has also studied the bronchopulmonary autopsy ma-
terial from 255 men and three women who died of lung carcinoma of
varying histological types, ranging in the spectrum of the WHO
classification (208) from the highly differentiated to the undiffer-
entiated squamous cell carcinoma, with others being oat cell, polygonal
cell, acinar, and adenocarcinoma. A search for double primaries was
made, and by using strict criteria, multiple primary invasive carci-
coma was found in 3.5 percent of the autopsies studied. When less

141


PERCENT Of SL/OES W/TH
CAR C/NOMA - /N - SI TU

IS.0

0.3 ,044 -1
   ,o.o.

Never Smotcd <l/2 Pack l/2-1 Pack l-2 Pocks 2t Pock8      Lull0
Requlorly     A Day   A ooy   A ooy    A oay       concw

FIQUBE 4.Pement of skides with cardnoma ire SUU.

Source : Auerbach, O., et al. [Table 2 (5), updated 1967].

strict criteria were used, but very doubtful cases excluded, up to 12.5
percent double primaries were found. This study suggests that multi-
ple primary bronchial carcinomas in the same patient may be more
frequent than previously suspected. Further studies are necessary in
this area, since therapeutic implications are also involved.

The differentiation of tumor types as related to smoking habits in
various groups with clinically diagnosed lung cancer has again been
investigated in several recent studies, In one study (19)) of 417 cases
of histologically proven lung cancer, 87 percent were smokers. Among
the squamous cell cancer cases 89 percent. were smokers ; among the
undifferentiated cell cancer cases 90 percent were smokers, and among
those with adenocarcinomas, 60 percent were smokers. A study (99)
dealing specifically with alveolar cell cancer of the lung reports that
91 percent of the 180 males in whom this tumor type was diagnosed
were smokers and, similarly, that 65 percent of t.he 85 females with
this type tumor were smokers. Another study (104) was made of lung
cancer cases in nonsmokers, defined as persons smoking not more than
one cigarette a day for 10 years. This study group included eight males
and 26 females. Of this group, only four patients had epidermoid
carcinoma (two males and two females). Both males had a history of
occupational exposure to respiratory irritants. Of the two women,
one had an unusual history of carcinoma, including multiple basal cell
skin cancers and &s& carcinoma of the cervix.

A study (1) was made of 666 histologically proven cases of lung
cancer. A smoking history was recorded on 442 of the men in this

142


series. The chart below takes into account smoking histories as re
lated to three histologic groups: undifferentiated, squamous, and
adenocarcinoma (see table 11).

TABLE Il.-Dihbuhn of lung cancer a%ath b celhlar tarpe and
           type of %?noking

           CellIllar type               N-oksr


Undifferentiated--- _ _ ____________________    4
Squsmoue-------------------------------       6
Adenocarcinoms- _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _      2


SOVIUX: Ashley, D. J. B., et al. [(f).Table 4.1

14       124
24       211
1       56

Insufllcient information is provided in this study to specify in detail
the past smom histories, but the data suggest that cigarette smoking
may be related to adenocarcinoma in some instances.

The preceding studies indicate that squamous, undifferentiated, and
oat-cell carcinoma rarely occur in nonsmokers. However, it appears
that cigarette smoking may also be associated with alveolar cell car-
cinoma and glandular carcinoma of the bronchi. This relationship has
been previously suspected. In fact as early as 1950 Wynder and Gra-
ham (206) demonstrated this relationship. This was also shown in the
study by Haenszel (30) . Greater standardization and precision of diag:
noses are needed to establish how few cases of undifferentiated or squa-
mous carcinoma occur in nonsmokers who have been established to have
never smoked appreciable amounts during their lifetimes. If 100 per-
cent accurate smoking histories were obtainable on every case of lung
cancer, it is suspected that very few cases of undifferentiated or squa-
mous cancer would be found in persons who had never smoked.
A report (08) on lung cancer in uranium miners noted a frequency
of lung cancer, occurring almost entirely in the cigarette-smoking
miners, greater than the frequency to be expected in a similar sized
cigarette-smoking nonuranium mining population. A recent report
(86) on bronchogenic carcinoma in asbestos workers also noted an in-
creased frequency of lung cancer, occurring entirely in the cigarette
smoking asbestos workers. This frequency was greater than the fre-
quency to be expected for a similar population of cigarette smokers
who were not asbestos workers. These reports suggest that cigarette
smoking may interact with certain other environmental exposures to
increase the frequency of lung cancer occurrence still further.
Analysis of occupation and other environmental exposures must be
performed simultaneously to detect which interactions with smoking
seem to be especially dangerous.

143


E-AL ~~~~ONARYC!ARCXN~GENESIS

Experimental attempts to produce lung cancer involve the admii-
istration of tobacco smoke condensates and of carcinogens known to be
present in tobacco smoke, either isl vitro to preparations of cells or
i% wivo in experimental animals. Difficulties are encountered with the
viability of tissue cultures and experimental animals when subjected
to these various substances. Studies of human tissue from lung cancer
patients indicate that abnormalities of the tracheobronchial mucosa,
such as loss of cilia, basal cell hyperplasia, squamous metaplasia, and
cellular atypism are important in the pathogenesis of human lung
cancer caused by smoking. These changes have been experimentally
produced in dogs exposed to cigarette smoke through a tracheostomy
(a, 70,80). A large number of dogs is now beii studied to determine if
lung cancer can be experimentally produced by this technique; if the
dogs continue to smoke for a longer time, malignant changes may ap-
pear subsequent to the already noted premalignant changes. The squa-
mous metaplasia involved in the premaliiant changes may explain
why cigarette smoke condensate most readily produces cancer in the
squamous epithelium of the skin of laboratory animals.

ADDITIONAL EVIDENCE CONCERNING E~PERIME NTAL ~ARCINOGENESU

The inhalation of tobaccoamoke by mice was reported to increase

the frequency of glandular tumors (37, &, 63, 70). Syrian hamsters
exposed to cigarette smoke developed a small number of tumors in the
tracheobronchial epithelium (30, 110). Cigarette smoke condensate
has been studied in tissue culture preparations (88)) and implantation
of cigarette smoke condensate exposed lung tissue subcutaneously has
been reported to cause malignant growths (las) . Cigarette smoke con-
densate also causes skin tumors when applied topically (9, II, .#I, 48, 61,
74,89,207,108). This was confirmed by a large-scale study with ahout
8,000 mice by the Tobacco Industry Besearch Council of England (%3) .
Repeated injections of cigarette smoke condensate in rats produced
sarcomas (8$,82?,,&4 84). Since 1963 two studies have reported nega-
tive results when cigarette smoke condensate was administered intra-
tracheally to rate and Syrian hamsters (J?%, &) , respectively.
Bronchoscopic painting of cigarette smoke condensate rapidly causes
squamous metaplasia in dogs and may accelerate carcinogenesis (91).
Carcinogens, known to `be present in tobacco smoke, have been applied
to cells in tissue culture with the observation of malignant changes (7)
and other effects (H) , such as differential growth inhibition of normal
but not malignant cells ($3). Inhalation (63: 78, 90)) intratracheal
administration (a, 96, @?, 64,81), subcutaneous, intraperitoneal and
intravenous injection, oral administration, and skin painting of c&r-
cinogens have all induced pulmonary tumors (87).

144


The search continues for an experimental animal system in which
the inhalation  of tobacco smoke will produce malignant
tissue changes closely approximating those observed in human
pulmonary cancer. When dealing with passive inhalation of tobacco
smoke, however, a problem of the defensive barrier of the nasal passage
is introduced. SO far, dogs inhaling cigarette smoke through tra-
cheostomies seem to be the most promising system, but there are
problems in keeping the experiments going for the length of time nec-
essary for lung cancer to develop. Additional research is needed using
cultured lung tissue together with autograft and homograft studies to
determine in &uo results. Additional insight may thus be gained into
in &JO systems. It should be noted, however, that it may not he possible
ever to achieve histologic identity in pulmonary cancer production, not
only because of di&ulties in duplication of man's smoking aotion for
reasons of anatomic and physiologic differences, but also hecause of
inherent species' differences in cellular response.

CANCER OF THE BUCCAL CAVITY AND PHARYNX (LIP,

MOUTH, THROAT)

The Surgeon General's 1964 Report concluded that the causal re-
lationship of pipe smoking to the development of cancer of the lip
appeared to be estabIished. Although there were suggestions of a
relationship between cancer of other specific sites of the oral cavity and
the several forms of tobacco use, their causal implications could not be
stated at that time.

The National Center for Health Statistics (94) reports that during
1964,28 female and 15'7 male deaths occurred from cancer of the lip.
During the period 1959-64, male mortality from this disease declined
about 6'7 percent. This was partially due to changes in the diagnostic
classification but was mainly due to increased early diagnosis and
therapy. During the period 1953-64 when the seventh revision of the
International Classification of Diseases was in use, total mortality
from cancer of the lip remained about the same, but when analyzed
by age, substantial decreases occurred in this death rate for each lo-
year age group from 55-34 years.
As for cancer of the oral cavity, other than the lip, the total death
rate showed no marked variation from 1950-64 (3.1 and 3.3 deaths per
100,000 population, respectively). In 1964, the death rate for cancer
of these sites in the male population was about three ,times the cor-
responding rate in the female population (5.1 and 1.6 deaths per
l96,ooO population, respectively).

145


MORTAR DATA Ihoar TEE LARGE PROSPECITVE STUDIES

Hammond (.@) has reported data for males having cancer of the
buccal cavity or pharynx, as the underlying cause of de&h, by mor-
tality ratio and age-standardized death rates (table 12).

TABLE 12.-Buceal cuwity and pharyngeal cancer morh&y ratios and
death rates for male smokers, by type and spec@d age groupa

I                     I

                               cigarettes     mP=Ot

              4s-tEhn  a&F&  .52i~w
-                                                        -

Mortality ratio- _ _ _ _ ___ _______ _____      9. 90       2 93       4.94
Death rates- _ _ ____________________   1 (1) 8    `(7) 20    l(3) 15

1 Numbers in parmthesea indicate death rated of pmom~ who had never smoked ckamttss mgakly.
Somcle:H-ond.E.C.(#I).

The Dorn study (49) also has provided information with relation to
amount and type of smoking on males dying from cancer of the buccal
cavity and pharynx (table 13) :

TABLE 13.-Bwcal cavity aid plrarllngeal c(c1Ecer mortality rat&w and
de&h rates for U.S. veterans, by age, type, and amount of .wn&ing

ounwlt amokela of dgamttea only

Numbefofcl@rettedperday

-




I

I-

-i-

  0
I I  1-e
--

Buccal Cavity:

Mortality ratlo _--__---_--____-__   1.00
Death rated:

   Ape 46 to Mm _ _______________ _______,
    Age&St064 __-__--_--_-___-_ 2
    AgeBStoX ._-__-----_-_____ 4
    Age 76 plus _____-____________ _______,
Pharynx:

Molwltymtlo _____--___________ 1.00
De&b rated:

Agesat ____---------- _-- ---____.
Age 6R to 74 ____.____________   1

0. so

a
---___-.
---_---.

7.11

0
12

Sonrtcr: U.S. veterans study [app. table A (@)I.

lo-20 21-39
---



2.98 7.84


.--_... __-----.
6    12
10 19
..-.m-. . . . ..-..

l2.81 14.50

8     R
22    10

.-






. .



_ -








-

10+




6. 7a


_.-___.
0
9
. . - _ _ _.

10.84

_. _ _. _.
a9

--



a. 89   4.11

23   -.-..._.
6     8
15    la
12    aa


8.08 _..__...

2   . - - _ .- .
4   ._._._..

a. ii

97
2
11
__--...

LOB

._....-
4

The Canadian pensioners study (8) has not reported separately on
deaths from cancer of the buccal cavity and pharynx.
The Doll and Hill studies (%!?, 88) of British physicians have re-
ported on cancer of the mouth and pharynx, including cancer of the
nose (table 14).

146


TABLE 14.-De& rates fnnn cancer of upper mqiratoq tract and
     digestive system by site and type of smoker

           site

--

  Non     All
-Ii zi%z -    Pi or
amokem mlokm!          smokaa  &
                             SmOkU?l

Mouth, pharynx, or no88 _______     0      6      5     10       4
~mynx or trachea ._...._.._._.     0      6      5      3      10
Esophagus...--...-.-..-.-..-.     4     10      6     19      8


SOUEXE: Study of British phy8icfaw, [table 12 (WI.
Data on the relationship between amount of cigarettes smoked and
the death rates were also provided (see table 15).

TABLE K-Death rates from cancer of upper respis~ tract and
     digestive q&m by & and amount .smoked

ma

    Ameant of tobacco amokfd dafly @.I '


Non- AU    l-14
smokfllg amcwlbl    1s-z4 =+ %r
                      --

Mouth, pharynx, or nose . .._._.._ -l-i-Ii
                        0 7 4     1    21     6
Larynx or trachea _.._...._ . . . . . .      0    6 2 2 15     5
Esophague--....-.-......--..-.     4   12 8 14 20     2

I (9.) = 1 gm.-1 cigarette per day-x oa. tobacco per weak.
SOWCE: study of British physictam [table 13 (I?a9].

Additional significant information comes from a study (69) of 102
cigarette smokers, all of whom were %mred" of a primary mouth or
throat cancer and remained asymptomatic for at least 3 years. Of these
patients, 37 stopped smoking while 65 continued. Of the 37 who stopped
smoking, only two had a second primary cancer develop in a different
site in the buccal-pharyngeal area, whereas 14 of those who continued
to smoke developed a second cancer in a different site in the buccal-
pharyngeal area.

In one study (66)) pipe smoke condensate was dissolved in sputum
and applied behind the ear of mice. Although no ear lesions were ob-
served, two animals developed scirrhous and planocellular cancer, re-
spectively, of the lower jaw, perhaps as a consequence of licking the
ears of other mice. In another experiment (37), rats were placed in
chambers and exposed to cigarette smoke. Five of 68 surviving rats
developed tumors of the buccal mucosa, three of these animals had
malignant invasive lesions.

147


In another setting (g7), in which the oral area of mice was painted
with cigarette smoke condensate for 15 months, no lesions were noted
in the oral cavity. However, a sign&ant increase in lung tumors,
lymphosarcoma, leukemia, and reticulosarcoma was observed.

The Surgeon General's 1964 Report established the causal relation.
ship of pipe smoking with lip cancer, but did not find sufficient evidence
for a causal relationship of specific forms of smoking with canceFs
of other sites in the oral cavity and pharynx. Current informatiou
strengthens the association between the various forms of smoking and
the general category of cancers of the buccal-pharyngeal area but
present information remains inadequate for a judgment of causality.
Knowledge of the interaction of smoking and other factors known or
suspected as causative agents, when available, could assist in such a
iudgment.

CANCEROFTHELARYNX

The Surgeon General's 1964 Report concluded: "Evaluation of the
evidence leads to the judgment that cigarette smoking is a significant
factor in the causation of laryngeal cancer in the male."
The National Center for.Health Statistics reports (94) that 2,494
deaths attributed to cancer of the larynx occurred in 1964, as com-
pared with 1,852 deaths in 1950, a 34 percent increase. Almost all these
deaths occurred in. the male population, with a male-to-female-ratio of
about 8 to 1. T-he total death rate in 1964 was 1.3 deaths per 100,000
population, which represented only a slight increase over the death
rate of 1.2 noted in 1950. The mortality impact of this disease occurs
primarily af.ter middle age, there being a five-fold increase in the death
rate for males over 75 years as compared to males under 55 years of
age*

The Hammond study (40) repor@ the following information for
laryngeal cancer deaths of males with a ,history of regular cigarette
smoking, in terms of mortality ratios and death rates:
TABLE 16.-Luryngeai cuncer mortdity ratio8 and death ratmjormde
      cigar& 87noker8, by spec$ed age group8

                                     I           I
` Numbers In parsnthesm imlhta death ratea of pemoru who had never smoked cigarettes re4mlarl~.
8omux: Hammond. E. C. [table 24 (#I)].

148


The Dorn study (.@) of US. veterans reports the following laryn-
geal cancer mortality rat.ios related to amount of cigarettes smoked,
and smoking of pipes and cigars, or cigars only.
TABLE 17.--laryngeal cancer mortality rat& and death rates for U.S.
      mterans, by age, type, and amount ojmmking

     NumbezofcIgemtWpad~y    22 =s
  0     l-9    1MO     n-a    lo+ ebar
--- --

>Iortality ratio--- _ _ _ _- _    1 3.27 8.45 13.62 18.85 7.28 10.33
Death rates:
  Age 55 to f34--- ___ _    1 __---- 4 5 20 4 3
  Age65to74 _______ - _____ 7 13 17 -----__- 12 20
  Age 75 to 84-- _____   13 _---_____-______________________________

SOUROR: U.S. Vetemm shdy [app. table A WI.

The Doll and Hill study reported their data in terms of cancer of
the larynx or trachea (see tables 14 and 15) for relationships with
type and amounts of tobacco smoking.
The Canadian study did not provide separate data on cancer of
the larynx. No additional information has become available, since the
Surgeon General's 1964 Report, relating the several forms of smok-
ing, i.e., cigarettes, cigars, and/or pipes, to specific laryngeal cancer
sites (intrinsic versus extrinsic larynx).
The study previously referred to (69) which analyzed the develop-
ment of second sites of cancer after cure of a. primary oral cancer,
reports that of 37 smokers who stopped smoking, none developed
cancer of the larynx but that four of 65 continuing smokers developed
cancer of the larynx. Although small numbers arB involved, beneficial
aspects of smoking cessation are suggested.

Additional epidemiological evidence supports the previous con-
clusion that cigarette smoking is a significant factor in the causation
of cancer of the larynx.

CANCER OF THE ESOPHAGUS

The Surgeon General's 1964 Report concluded: "The evidence on
the tobacco-esophageal cancer relationship supports the belief that
an association exists." However, the Committee at that time noted
that there was not adequate data on which to base a decision as to
whether the relationship was causal.

The National Center for Health Statistics (94) reports that from

149


1950 to 1964 the mortality from cancer of the esophagus rose about
8 percent in the male population and 9 percent in the female popula-
tion. In 1964, males had a death rate for esophageal cancer that was
3.7 times higher than the female rate. The greatest relative increases
were in the age groups under `65 years, especially the age group
3544 years.

MORTALITY DATA FROX THE LARGE PR~SPECI~VE STUB=: The Ham-
mond (40) study reports the following death rates and mortality
ratios for males in the age groups 45-64 and 65-79 who have a history
of smoking regularly:

TABLE 18.--E8opha@z.l cancer nwrta.ltiy rat& and deau ratm for mule
       Cigar@ 8??der8, by Sp&fi age grvup8
                I  b@- I  see 66-n

Mortalityratios------ _______ -_-_--_-- __________      4. 17       1.74
Deathrates------------------------------------ 1 (1) 4     ' (4) 7

1 Nombem in parentheses IndIe& death rates of penrom who have never smoked -3'.
Souacn: Hammond. E. C. [t&b 24 (4O)l.
The Dorn study (49) reports the following mortality ratios and
death rates in rslation to number of cigarettes smoked per day plus
other forms of smoking:

TABLE 19.~Esophageal cancer mortdity ratios and death ratea for
     U.S. veterans, by age, type, and amount of smoking

                  Number of c&a&tea per day       Pipe
                   $rg yg Pipe
                 0     14    10-20    21-m 40+
              ----        -----

Mortality ratios- _ _ 1. 00  1. 76  4. 71   11.50 7.65 4.05 6.33   1. 99
Death ratea:
  Age 55 to64--- 1 2 5     14    9 5    8 ----_-
  Age 65 to 74-w    3 ______ 16     25 10 20 23    18
  Age 75 to 84--w   45 --------------------------   41 ------ 72

Scmac~: U.S. Vetwane study [app. table A (@)I.

The Canadian veterans study did not give separate information
about deaths from esophageal cancer.

Autopsy studies of smokers as compared with nonsmokers, spe-
cifically observing the pathological changes in esophageal tissue, have
been performed by Auerbach (3). A microscopic study was made of
12,598 sections of esophageal autopsy tissue from 1,268 men, who died
from causes other than esophageal cancer. The smoking histories were
recorded ,but not known to the person examining the slides. The find-
ings were strikingly similar to the abnormalities generally accepted as

150


representing premalignant tissue changes in the respiratory t&t
cpithelium. Esophageal epithelial cells with atypical nuclei were found
far more frequently in cigarette smokers than in nonsmokers. The Mm
"atypical nuclei" describes nuclei with an irregular distribution of
chromatin. Other abnormal changes including giant nuclei may also be
present. Basal cell hyperplasia and hyperactive glands also were found
more frequently in cigarette smokers than in nonsmok8rs. An in-
crease in frequency with amount of cigarette smoking was noted for
both epithelial cells with atypical nuclei and basal cell hyperplasia.
Atypical nuclei in epithelial cells were also more frequently found
in ex-cigaretts smokers as compared to nonsmokers. Tables 20 and 21
illustrate the frequency of these Cndiigs:

TABLE 20.-Atypical nuclei in basal ce& of qithelium of esophugw of
        mda, by smoking habits and q

Atypical nucld

A. *I& YUN

Number men- _ _____________.
Total m?tlOM 1- ____________.
No atypical nuclei -_________,
Some but <@I penxnt
atypical _--_ _-----__----_--.
60 percent or more ntypical..

1. YEN VNDUB ME SC

Number men- _ ________ _____
Total sections I- ____________
No atypIcal nuckL ________
Some bat <59 percent
atypical _-_-__-- ---___---_-
60 percent or more atypical--

C. YIN AOED SO-60

Number IMXI. -__-___--_____
Total s&Ions I- ____________
No atypical nncleL. _ ______
&me but ~50 percent
atypically__ _- _ - - _ _ _ _ _-- _ _ _
60 percent or mom atypical..

D. MEN AQED 70 OB OLDEN

Number men- _ _____________
Total sectIona L _ ___ _____ ___
No atypical nuclei.. ________
Some but <@I percent
atYpical .-------__________.
60 percent or more atypIcaL

-

-

N

--









- _.









.-
._





._
.-
._


.-
.- -

91
7a7
7a3

52
2





26
228
190


a3
.___-




u
a7c
372

  1
  I





21
181
174

11
*-__.

Per-

-

___--
00.0
98.1

a0
0.3





.___-.
100.0
35.2

14.9
.____.





.___-.
lUJ.0
93.4

1.1
0.5





_-mm-
190.0
91.9

a 1
__-e-

Current
dgerette

-


1

779
II752
157

i,ssO
I, 198

1 Sections with some epithelhun present.
80vrcE: Auerbwh, O., et al. [table Z(s)].

Per-
tat
-

.-__--
lOa
2.5

79.8
17.7





.____.
100.0
3.4


OaO
6.6





_____.
100.0
2.2


75.6
22.2





-w-s-
190.0
1.5


74.0
24.5

,-

b

.-






1

Ex+aretta

181
LM
770

763
51





26
256
56


196
  7


EI
461

452
(0





U
375
253


118
  4

-

-



.-




_.
I









_.

-

zi
-

.__-_.
100.0
49.5

43.3
3.2

i----'
LOO.0
21.7


75.6
2.7

-e-m-
laI.0
43.4


47.4
4.2





-----
199. a
57.4


31.5
1.1

-

`k-
-




89
763
5a


lB3
25





  9
77
  1

74
  2





36
310
87

ml
12





42
879
15


853
11

-

2
-

.-_-_.
100.0
3.9

89.8
a3

___-_.
lo(LO
i.a

9al
2.6

-_--_
100.0
11.9

34.2
a9

__-_-
100.0
4.0

93.1
29

-

r

other

k?
-

31
266
74


176
4

I
218
117

9a
  a

-

PSr-
cent






-__--
loo.0
37.4


ea7
1.9





,_--_-
lo(10
7.5

86.8
5.7





.____-
109.0
23.9


39.5
1.6





.__---
199.0
54.9

43.7
1.4





161


TABLE 2l.-Atypid nuclei in b&Sal Clue of epithdium of esophagus of
      mu&a, by amount of smoking and age

Ce& with atypical nualel

<I P& I Is+-
         l-2 Pwka

I I
"A?- pg-
      CMt

A. All a#ee __________________________
  Total Metions 1----_-------------
No atyphl no&l ________ __ _-me
Nonlo but <No peArcent atypica.
6Opemntormoreatyplcd~--..-
B. Men ander sge 60:

  Numk mea ___________________
  Total eectloM ' -_-_____----------
No atypical nneleL. ___________e-
8ome but CEO pfrcmt atypicaL
60 paeut or mom atypical __-_-_ __.
c. Men aged m88:

  Number men ________ ____________
  Total SsctioM f -_-____-----------
No atypkal made4 ____________ ___
&me but <60-t sty&al.-
6OpercantormomatyplcaL~~~~~
D. Men aged 70 or older:

Nnmbsrmen---------------.---
Total aectlone I__________________
No atypIcal nnclel. ___________ ___
8ome but <tHl parant at&caL-
80 pemnt or mom atypical ______ __.

01 I
   - _ -- - --,
787 lmo
7aa   82.1
I    a6
a    0-a

23 _ __ _ -- _,
22a lmo
190 85.2
an   14.8
-- - _ _ __ _ _ _ _ -

44 _ _ - - - - -
a79 `imo
an   oar
4    1.1
2   -0.6


21 _-_----
la5   lmo
170   91.9
16 a1

. _ - - - _ __ _ _ - _-

__I-

"E-   Pa- Num-
  I       Ps- Num-
     cent be?  I
               ocnt be

a

0
780
8z
85

88
am
11
286
48

-- _ - --- _   U8
rmo boa9
  6.8    m
86.8  $867
  7.4.   8a8

._------   187
imo i,s79
1.1 a9
81.6 1.001

17.4

--___- --   la2
imo  1.18
11.1    11
88.2  LOB9
  a7 6@

._-_-_--
loo.0
1.8
oL2
6.0

----_---   240  .___----
imo  236   imo
  2.8    18    QS
87.9  l.bQI   76.B
  aa    491   22.2

_--_-- --    U
imo ~4
  a4 ______-.
ma ml
14.a    8a

._------
imo
._____--
76.9
24.1

l-

449

68
467
2
s82
n


111
w8
a6
8l4
299

19
174
2
911
77

-

Per-
ceut

.----__
imo
0.4
88.0
rao

.--mm__
imo
a7
04.8
al.a

.----__
imo
Ll
64.7
44.2

* 8ectioua wtth some eplthelium preaant.
8oosct: Anerbach, 0.. et al. [table S(s)].

Because of the association noted between esophageal cancer and
alcohol consumption reported in the Surgeon General's 1964 Report,
a study (68) was undertaken to consider the possibility that the car-
cinogens known to be present in tobacco smoke could penetrate esopha-
geal tissue more readily, if dissolved in aqueous solutions of ethanol.
Mice were exposed to several compounds by intraesophageal tubation.
Tissues were then removed and studied by fluorescence microscopy.
Deeper penetration and a different distribution were found when
benzo (a) pyrene was dissolved in aqueous solution of ethanol as com-
pared to benzo (a) pyrene dissolved in olive oil. It was also found that
benz (a) anthracene and fluoranthene dissolved in ethanol solution or
aqueous caffeine solution could penetrate the epithelium of the
esophagus.

The present evidence strengthens the conclusion that a four-fold to
five-fold increased risk of dying from esophageal cancer is associated

152


with tobacco smoking. Autopsy evidence indicates that smokers have a
greater frequency of pathologic changes of the esophageal tissue, some
of which are generally considered to be premalignant. It has been
demonstrated that known carcinogens such as benzo(a)pyrene and
others can penetrab the esophageal tissue when dissolved in aqueous
ethanol or caffeine solutions. The present evidence suggests that smok-
ing may be a causal factor in the development of esophageal cancers,
but is still insufficient for a firm judgment of causality. More informa-
tion on alcohol as a confounding variable and/or interactant is vitally
needed.

The data on women for the preceding categories of buccal, pharyn-
geal, laryngeal, and esophageal cancers have not been reported due to
the relatively too few cases involved. However, Hammond has pooled
these data into one group. Table 22 shows an increased mortality ratio
in this overall combined category but the number of deaths is still too
small for significant conclusions to be drawn.

TABLE 22.-Mortaht3,1 ratios and age-standardized death rates for cancer
     in uromen aged 46-64 by site and amount smoked

Buccal cavity, pharynx, larynx,
and e8OphfbgU8- __ ___ ___ _ __ _ _     2      3
Lung'-------_-______________     7     15
Pancreas- _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _     6      11

~Bmoked2oormomcl r tteu
day. and begm smoHng     a day regardlw of
            fore age 25.    afe,boganmokingommokod10ormomcigerottess
                   1 E~olading raobea, pleura.
Some: Hammond, E. C. [table 26 (@)I.

Table 22 also shows the dosage effect of smoking on women, for dif-
ferent cancer sites-an increased amount of smoking being reflected
in an increased mortality ratio.

CANCER OF THE URINARY BLADDER

The Surgeon General's 1964 Report concluded: "Available data
suggest an association between cigarette smoking and urinary blad-
der cancer in the male but are not sutlicient to support judgment. on the
causal significance of this association."
The National Center for Health Statistics (84) reports that there
has been no change in the death rata from cancer of the bladder and
other urinary organs during the period 1950-64. For 1964, the male


death rate from this cause was 5.8 deaths per 166,600 population, and
the female death rate was 2.6 deaths per 166,606 population.
The mortality data from the large prospective studies are presented
below. The Hammond study reports the following mortality ratios and
death rates for cancer of the bladder and other urinary tract sites, for
males, by history of regular smoking :
TABLE ,23.-Hudd-er cuncer rnmtdty doe and age-&ndurdized
dath rate8 for mu.+% cigar& tvnokers, by sped&d age group8

                                     cfgamtte smokem

                                    Am-     Am 6S-79


Mortslityratioe_______________-----------____-_      200
Deathratee--___-------------------------------    ' (417  (1%

`Nnmbppsinpsrentheaeaindiostedsath~for~w~hr~never~kedregalsrly.
Sonncr: H ammond, E. C. [table 24 (@)I.
The Canadian Pensioners study (8) included bladder cancer in
t-he general category of genitourinary cancer.
TABLE 24.-l&&tuutirucry cancer mortu&y ratio8 for Canadian vet-saw
        by age and amount smoked

          Mortnllty ratioa             Number of dg8mttm emoked per day


                                     14       10-20      2l+


Allage. -------__----------------------.- 1.33     l.44      L 43
Age 70+ ________________-___------------     L 10     2 24      2. 43


80umx: Canadiau jmn&nere atudv [table 83 (e)].

The Dorn study of U.S. veterans (.@) reports the following mor-
tality ratios and death rates for males by quantity of cigarettes
smoked per day and by pipe and/or cigar smoking:

TABLE 25.-Bladdm and other urinary tract tamer nzortu@ ratios and
deuth ratm .for U.S. vetmam, by age, type and amouni smoked

Number of c&amt.tee smoked pw day

Mortality ratios- 1. 00
Death rates:

1. 10   1. 93   a 20   2 52 1.09

Age45-54 ______ - __________ 13    18    - - - - - - - - - - - - _
Age 55-64-m- 8                    20
Age 65-74-m 22 2: :: : 45 ::
Age 75-84-_- 89    _________--_____---------__-_-____

80~~1: U.S. veternne atady @pp. table A (ro)].
154

`aam pm


--

a 94 1.20


_----_ m---e
1s 14
9 28
_----- ---- l-


The Doll and Hill survey of British physicians ($38) reports the
following standardized death rates for cancer of the urinary bladder :

TABLE 26.-Death rates fat cancer of urinary bZu&?w by type of m&kg

-

Nonsmokers                   Type of mlokklg


             All smokers    cfgarette smokera   Mfred mnokm Pfpeorcigammokera


   17         11         13         12          7


8owcr: Study of British phyaicha [table 14 (!?@I.

TABLE 27.-Death rates for mncer of urinal b,!ad,&r by amount smoked

Amount of tobecco mloked d8uY (e.)'

   I-14          1624          25+


   10         11         13



k 1 g.- 1 cigamtte/d8y- $440~ to~~w~.
Souncr: Btudy of Brltfsb &`skhm ttable IIl (#@I.

All amowltd       CMtfOll


  12          8

The Hammond and the Dorn studies report mortality ratios of
more than 2.00 in smokers of more than 20 cigarettes per day. The
Canadian study reports the same for men over 70 years, who smoke
more than 10 cigarettes per day. The Doll and Hill survey is incon-
sistent with those pre%iously mentioned in that it shows no association
between type of smoking or amount smoked and bladder cancer.
However, this survey consists of but 38 deaths due to bladder cancer.
Two controlled retrospective studies of bladder cancer patients
have been reported since the Surgeon General's 1964 Report. In both
studies these patients had a significantly greater percentage of smok-
ers, the majority of whom were cigarette smokers, as compared with
controls. The first study (18) reported 94 percent smokers in the
patient group and 74 percent in the control group. When analyzed
by the amount smoked, the patient group had 80 percent heavy smokers
(greater than one pack per day for 30 years) as compared to only 45
percent heavy smokers in the control group. The second study (89)
reported 93 percent smokers in the patient group and 34 percent in
the control group. However, when compared by the amount smoked,
there was a much larger diflerence between the two groups; there being
86 percent of the patient group with a high "smoking index" (amount
smoked X duration of smoking) as' &&pared to only 66 percent in
the control group. The latter study also reported a greater frequency
of inhalation and "cigarette only" smoking in the patient versus
controls groups.

155


A~PSY STUDIES

There have been no reported studies analyzing changes in the
bladder tissue of smokers compared with nonsmokers. Studies of this
type would be helpful to determine if smoking is associated with
pathologic changes commonly thought to be premalignant in other
types of tissue.
           EXPERIMENTAL CARCIN~GENESIS

Cigarette smoke condensate ae well as several tobacco smoke con-
stituents were implanted with chol&erol directly into the bladder
of mice (26). Only hydroquinone produced a significant number of
bladder tumors.

METABOLIC STUDIES OF ENIWGENOUS CABGINOQENIC SUBEJTANCE~ IN B&N

Certain ortho-aminophenols and aryl hydroxylamines are lznown
to be carcinogenic (16). Three normal intermediate metabolites of
tryptophan are ortho-aminophenols (3-hydroxyanthranilic acid, 3-
hydroxy-2-amino-acetophenone, 3-hydroxykynurenine) and are known
to induce cancer when placed in the bladder of mice (lb).
Kerr, et al. (61,5!8), performed metabolic studies on six men, three
smokers and three nonsmokers. He found that the three nonsmokers
had substantially increased urinary excretion values of 3-hydroxyan-
thranilic acid and 3-hydroxykynurenine, after having smoked for 5
weeks, with a concomitant decrease in the excretion of N'-methylnico-
tinamide, a normal end product of tryptuphan metabolism. After hav-
ing stopped smoking for 5 weeks, the three smokers showed decreased
urinary excretion of these same intermediate metabolites and an in-
cream in N'-methylnicotinamide excretion.

The carcinogenic metabolites increased an average of 50 percent
while the normal end metabolite decreased an average of 34 percent
in response to cigarette smoking. A reversal was noted after the men
stopped smoking. These studies suggest that cigarette smoking changes
the normal metabolic pattern of tryptophan, leading to the accumula-
tion of carcinogenic metabolites in the urine. Further studies are
needed to confirm these findings.
Another study, designed to detect abnormalities of tryptophan (6)
in patients with various neoplastic or nonneoplastic conditions of the
urinary tract, showed that 29 of 201 bladder cancer patient,s had both
kynurenine and 3-hydroxykynurenine, in contrast to eight of 167 pa-
tients with other urinary traot diseases, neoplastic and nonneoplastic.
However, more renal cancer patients had 3-hydroxyanthranilic acid
than bladder cancer patients. This study did not include data concern-
ing current smoking habits of the patients studied.

156


The additional epidemiological, clinical, and experimental data
strengthen the association between cigarette smoking and cancer of
the urinary bladder, but are still insufficient to infer that the relation-
ship is causal.

CANCER OF THE STOMACH

The Surgeon General's 1964 Report stated that no relationship has
been established between tobacco use and stomach cancer. No new
evidence refutes this statement.

Epidemiological evidence does not show a significant relationship
between smoking and stomach cancer. The overall mortality ratios,
although greater than for nonsmokers, are smaller than for any other
disease related to smoking. There is also no gradient with the amount
of tobacco smoked.

TABLE 28.-Male mortality ratios and death rates for cancer of stumah
         by spec$ed age groups

                                   apsra-sr     &wm-7Q


Mortality ratios- ___ ___ ___ __ _ __ _ ____ -_ __ ______ __      1.42      1.26
Death rates------------------------------------ 1(11) 16    (57) 72

1 Numbers In psmntheaaa indicate death rates of persons who had never smoked cbrettea regalarly.
SOUSCE: Hammond, E. C. [table 24 (lo)].

TABLE 29.-Mortuiity ratios and d&h rates for cancer of stomach
    by age, type, and amount smoked, in U.S. wteram

Mortality ratio
(tohI) - - - - - - - - - - - _
Death rate:
  Age 45 to 54----
  Age 55 to64----
  Age 65 to 74-e-e
  Age 75 to 84----

0     1-Q   lo-#) n-a9 40+
-----

1.00 2.17 1.61 1.35 1.87

10 __----    7  - - - _ - - - - - - -
13 27 21 24 53
28 68 48 58 46
87 ___________________-___.

~OWCE: U.8. Veterans study [app. table A Wll.

157


TABLE

%I.-Death ratm for cancer of tttmach by type and amount
    moked, in British phy8Gam

Although cigarette smokers appear to have slightly higher death
rates from cancer of the stomach, the differences am small and do
not bear any consistent relationship with amount smoked.

CANCER OF TFIE PANCREAS

The Surgeon General's 1964 Report did not report on cancer of
the psncreas.
The more recent epidemiologic evidence shows an increase in the
death rates and mortalit
cigarette smokers (@,@ 3 ratios for  creatic cancer among male
          . Comparab y elevated ratios are noted for
           $"
females but not to the extent noted for males (@) (see tables 22,31,
32,33). Both theU.S. veterans study (M) and the Canadian pensioners
study (8) reveal a gradient of mortelity risk increasing with the
amount of cigarettes smoked. Data are ins&Gent to draw any con-
clusions for pipe and/or cigar smokers.

TABLE 3l.-Mon!u.&y 9~ztio8 an& d&h rata for cu?m~ of paweua
    by 8ex and epec+ age group8 in cigarette smokers

&o lb-79

                                           F-ale      Mob


Mortality ratio- _ __ _ __ _ _ __ _ ____                   ' 1.41     2. 17
Death rate ______- ______-__-___             * (32) 46   ' (31) 66



*Corn  tedkomap .tf4bie19.
~Nnmeralnpmen saa8indloabdaathrUesd~W~~nsosr~~desretteareeulerly.
  Pp &
louucr: Hammond, E. C. [tabled 24,28, and apt. tfatde 19 (@)I.

168


TABLE 32.-Mortality ratios and death Tales for cancer of pancreaa
     by age, type, and amount smoked, in U.S. veterans

                     C@rette3/daY         Mor cfgar
                    *I?        Pipe

                  0                       c&ars
                       l-9    1lMO   21-&l 40-k
                --------

Mortality ratio
(t&al)-. ---------- 1.00   . 87 1.93 2.18 1.87 1.13 1.52 .74
Death rate:
  Age45to54---------------- 9    21 __--__   26 ---------_--
  Age55to64---- 13 15 24   '26 21 19 21    22
  Age65to74---- 29 29 50 56 27 38 58 19
  Age 75 to &I----  109 ______ ________-_________ 32 33    68


sOwcr: U.S. vetemna study [app. table A (4@1.

TABLE X3.-Male mort&ty ratios for cancer of pawem of cuxrd
      cigarette smoker8 by amount mkeo?


                                  CWettsddsr


                            0        1-9       10-20       21+


Mortality ratio ________________     1. 00     1. 40     1. 96      2. 37


SOWQ: Cauadlan pensi~mra study [table 8.2 (8%

                RfisUlU5

An association between cigarette smoking and pancreatic cancar
is implied, but in the absence of data on other possible causal factors,
confounding variables and interactants, the signikance of this `BSSD-
ciation is not clear.


CITED REFERENCES

(1) ASHLEY, D. J. B., DAVIES, H. D. Cancer of the lung. Histology and blo-
   logical behavior. Cancer (Philadelphia) 20(2) : 185-174, February 1367.
(2) AUEBB~CH, 0.. HAMUOIVD, E C., Krw~rv, D., Gmrx?xx~, L. Emphysema
   produced in dogs by cigarette smoking. Journal of the American Medical
   Association (Chicago) lQQ( 4) : 241-246, Jan. 23,1Q67.
(8) AVI~UXACEC, O., STOUT, A. P., H~wdolpo, E. C., Ga~~~~rrrrrx+ L. Histologic
   changes in esophagus in relation to smoking habits. Archives of Environ-
   mental Health (Chicago) 11(l) : 9-16, July 1985.
(4) Aumm~crr, O., Sxvwr, A. P., H~h0dorm, E. C., G-KEL, L. Multiple
  primary bronchial carcinomas. Cancer (Philadelphia) 20(b) : 6QQ-765,
   May 1987.

(5) A~~BACH, O., Srorrr, A. P., FU~~YORD, E. C., GABMNXXL, L The role
   of smohing in the development of lung cancer. Proceedings of the
  National Cancer Conference (New York) 5: 4V7-661,1964.
(6) BE~TASSI, C. A., P~~~SSIM~TTO, B., Ar&rren~, G. The metabolism of tryptophan
   in patients witli bladder cancer and other urological diseases. Clinica
   Chimica Acta (Amsterdam) 8 : 822-83l, 1963.
(7) BEBWA~..~, Y., SACHS, L In vitro transformation of normal cells to tumor
   cells ,by carcinogenic hydrocarbons. Journal of the National Cancer
   Institute (Washington) 34(4) : 64l6!% October 1986.
(8) BEBT, E. W. R. A Canadian study of smoking and health. Ottawa, Depart-
   ment of National Health and Welfare, 1988 137 pp.
(8) BOCK, F. G., Moons, G. E., CLABK, P. 0. Carcinogenic activity of cigarette
   smoke condensates. III. Biological activity of refined tar from several
   types of cigarettes. Journal of the National Cancer Institute (Washing-
   ton ) 34 (4) : 481-493, April 1366.

(IO) BOCK, F. G., MOOBE, G. E., Caovca, 5. K. Tumor-promoting activity of
   extracts of unburned tobacco. Science (Washington) 145(3634) : 831-
   833, August 1984.

(If) BOCK, F. G., Mooas, (3. E., Down, J. E., CLUUC, P. C. Carcinogenic activity
   of cigarette smoke condensate. Journal of the American Medical Asso-
   ciation (Chicago) 181: -73, August 1962
(18) Boox, F. G., SEAXBEBOEB, R. J., Mxxns, H. K. Tumonr-promoting agents
   in unburned cigarette tobacco. Nature (London) 2QS(sMO) : 584-586,
    Nov. 6, 1985.

(IS) BO~ZIVF~EVIVD, E., Ksr~, M., SAXDEB~, F. K., Sxxs~~n~~o, 5. S., BE~DICH, A.
   Malignant conversion of cells in vitro by carcinogens and viruses. Pro-
    ceedings of the National Academy of Sciences of the United States of
   LLmerica (Washington) 56: 672-679, 1966.
(14) BOUTWELL, R. K., BOSCH, D. K. The tumor-promoting action of phenol and
   related compounds for mouse shin. Cancer Research (Chicago) 19:
   413-424.1969.

(15) BO~LAIVD, E. The Biochemistry of Bladder Cancer. Springfield, Ill., C. C.
   Thomas, 1983. 95 pp.

(16) BORLAND, E., BUSBY, E. R., DVKE~, C. E., Gxovmr, P. L., M~r?sor~, D.
   Further experiments on implantation of materials into the urinary
   bladder of mice. British Journal of Cancer (London) 18(3) : 675681,
   September 1984.

(17) CHEmcxs, L A., A~~~KAB, S. G., Vom~mrv, M N. Hepatic tumors induced
   by selenium. Bulletin of Experimental Biology and Medicine (Moscow)
   53(3) : 313-317, July 1983.

161


(18) COBB, B. 0.. Arwm~, J. 8. Cigarette smoking and cancer of the bladder.
   Journal of the American Medical Association (Chicago) lQ3(5) : v
   Aug. 2, 1966.

(19) COHEN, El., Hoeaanp, 8. Primary carcinoma of the lung. A rdeW of
   417 histologically proved casea Diseases of the Chest (Chicago) 49 (1) :
   87-74, January 1968.

(30) COOK, J. W. Tobacco smoke arid lung cancer. London. The Royal Institute
    of Chemrstry. Lecture Series No. 5,1Q61.18 pp.
(31) CBOCKEB, T. T., NIELBXX, B. I., LA~XITZKI, I. Carcinogenic hydrocarbons.
   Effecta on suckling rat trachea in. organ culture. Archives of Environ-
   mental Health (Chicago) lO( 2) : 240-250, February 1986.
(22) DAY, T. D. Carcinogenic action of cigarette smoke condensate on mouse
   skin. Au attempt at a quantitative study. British Journal of Cancer.
    (London) 21(l) : 53-31, March 1987.

(93) DIAMOIVD, L The &ect of carcinogenic hydrocarbona on rodent and
   primate cells In oitro. Journal of Cellular and Comparative Physiology
   (Philadelphia) 66 : 133-196, 1966.

(94) Dibenz(a,h)anthracene; No. 6333. In: PATTEBBON, A. M., CAPELL, D. D.,
   WALKEE, D. F., edltonr The Ring Index. Columbus, the American
   Chemical Society, 1960. p 919.

(2.5) DICKENS, F., JOHES, H. E. H., WAYNFOBTFI, H. B. Oral, subcutaneous and
   intratracheal administration of carcinogenic lactones and related sub-
   stances: The intratracheal administration of cigarette tar in the rat.
   British Journal of Cancer (London) 20 : 134-144, March 1988.
(86) DIPAO~, J. A. l3XCect of dgarette smoke condensatea on homografts of
   neonatal lung tissue in mice. Nature (London) 204(4Q34) : 11591161.
   Dec. 19,lfM.

(37) DIPAOLQ, J. A., Lvwm, Bf; L Tumor incidence in mice after oral paint-
   ing with cigarette smoke condensate. Journal of the National Cancer
   Institute (Waehington) 34(5) : 5QtXO0, &lay 1968.
(88) DOLL, R., Hw A. B. Mortality in relation to smoking : Ten years' ob-
   servations of British doctors. (Part 1) British Medical Journal (London)
   1 ( 5395) : 13QQ-1414 May 30,lQtM.

(23) DOLL, R.., HXLL, A. B. Mortality in relation to smoking: Ten years' ob-
   servations of Brltlsh doctors. (Concluded) British Medical Journal
    (London) l(53Q6) : 14@%1467, June 6,lQtM.
(30) DONTBNWILL, W., WIEXMXTKE, B. Tracheal and pulmonary aberrations fol-
   lowing the inhalation of cigarette smoke by the golden hamster. Iti:
   Severi, L., editor. Lung Tomoti in Animals. Perugla, Italy, Division
   of Cancer Research, University of Pemgia, June 1988. Pp. 51Q-526.
(32) DBUCK~EY, H., SCHIJBA~IZ, A. Quantitative Untersuchungen zur Bedeu-
   ting des Benepyrene filr die carcinogene Wfrkung von Tabakrauch.
   ZeMschrift ftir Krebsforschung (Berlin) 65: 465-%70, 1983.
(83) DBIJCKMX, H., SCHMHHL, D., BENTHNEZ, H.. MUTH, F. Vergleichende
   Prtifung von Tabakrauchkondensateu, Benzpyren und Tabak-Extract
   auf Carclnogene Wirkung an Ratten. Naturwissenschaften (Berlin) 47
    (24) : t3ow306,lQML

(3-S) Fmmx, E. S., BAI%AT~A, E. J. Polonium-210 in tobacco, cigarette smoke, and
   selected human organs. Public Health Reports (Washington) 81(2) :
   121-l27, February lQ5Q.

(3)) FI-IXHUQH, 0. G., NEMON, A. A., BL.IS~, C. I. The chronic oral toxicity of
   selenium. Journal of Pharmacology and Experimental ,Therapeutics
    (Baltimore) 80(3) : 23fMQQ, March 1944.

162


(95) GBEOOBY, L. P. Polonium-210 in leaf tobacco from four countries. Science
   (Washington) 150: 74-76, Oct. l,lQ65.
(36) Garcrum, L. Experimental pulmonary adematosis in rabbits. In: Severi,
   L., editor. Lung Tumors in Animals. Perugia, Italy, Division of Cancer
   Research, University of Peru&a, June 1988. Pp. 78%7Q7.
(57) GVE~IN, M. Tumeurs pulmonaires et cancer buccal ches le rat wumis a
   l'inhalation de fum& de cigarette. Bulletin de I'Amociation Fnancaise
   pour 1'Etude du Cancer (Paris) 46: w, 1969.
(38) GUIMABD, J. Alt6ratdons ceilulaires provoqu& in uitro, sur dea flbrob~
   quiescents et en mitose, par des goudrons entires de tabac. Archives des
   Sciences Physiologlques (Paris) 20 : 153-167, 1968.
(39) HAENSZEX., W., SHIYKIN, M. B., MANTEL, N. A retrospective study of lung
   cancer in women. Journal of the National Cancer Instltuti (Washington)
   21: 825-842, 1958.

(40) HAMMOND, E. C. Smoking in relation to the death rates of 1 million men
   and women. In: Haenssel, W., editor. Epidemiological Approaches to the
   Study of Cancer and Other Diseases. Bethesda, U.S. Public Health
   Service, National Cancer Institute Monograph No. 19, January 1986.
   Pp. 127-204.

(41) H-8, R. J. C., NEXBONI, G. Cigarette smoking and the induction of lung
   cancer in mice. In: Severi, L., editor. Lung Tmnors in Animals. Pemgia,
   Italy, Division of Cancer Research, University of Pemgia, June 1968.
   Pp. 497-512.

(48) HEBaaD, K. M. The effects of benw(a)pyrene, cigarette smoke condensate
   and atmospheric pollutants on the respiratory system of Syrian hamsters.
   Acta ; Unio Internationalis contra Canerum (Louvain) 19 : 71&714,X%3.
(MS) HILL, C. R. Polonium-210 in man. Nature (London) 208(5OOQ) : -8,
   Oct. 30, 1965.

(-(4) HOFFMANN, D., WYNDEB, E. L. Beltrag mr Cardnogenen Wirkung von
   Dibenzopyrenen. Zeitschrift fiir Krebsforschuug 68: 1374. X%6.
(45) HOFFXARR, D., W~IVDEB, E. L. On the isolation and identification of poly-
   cyclic aromatic hydrocarbons. Cancer (Philadelphia) l3(5) : 1062-1073.
   September-October 1960.

(46) HOFFMANN, D., WYRDEB, E. L. The reduction of the tumorlgenicity of clga-
    rette smoke condensate by addition of sodium nitrate to tobacco. Cancer
   Research (Chicago) 27 : 172-174, January 1967.
(47) HOFFMANN, D., WYNDEB, E. L. The tumor initiator in tobacco smoke
   (Abstract). Proceedings of the American Association for Cancer Re-
   search (Chicago) 7: 32, April 1986.

(48) HOYBU~EB, F., TBEOEB, A., BAKEB, J. R. Mouseskin painting with smoke
   condensates from cigarettes made of pipe, cigar, and cigarette tobacco&
   Journal of the Natlonl&i Cancer Institute (Washington) 31(6) : 1445-
    1459, De&ember 1963.

(69) KAHN, H. A. The Dom study of smoking and mortality among U.S.
   veterans : Report on 8% years of observation. In: Haenewl, W., editor.
   Epidemiological Approa&a to the Study of Cancer and Other Diseasea
   Bethesda, U.S. Public Health Service, National Cancer Institute Mon+
   graph No. 19, January 1966. Pp. l-125.

(59) KELLER, T. F. Polonium-210 content of mainstream cigarette smoke.
   Science (Washington) 149: 537-538, July 30, 1965.
(51) h, W. K., B-IX, M., -8, P. E., Woo, 8. K. C., MKNCZYK, z. The
   elX%t of cigarette smoking on bladder carcinogens in man. Canadian
   Medical Association Journal (Toronto) Q3( 1) : l-7, July 3, 1966.

163


(52) 9EBB, w. K., BABKIN, X6., LEVBBS, P. 1, WOO, 9. K. C., Mmoli~g, I* The
  effect of smoking on bladder carcinogens. Presented at the Meeting of
  the Se&on of Urology, the Academy of Medicine of Toronto, T!oronto,
  Ontario, Cauad~, Mar. 16,lQtlS. [Unpublished.] 3 pp.
(53) KINOEUTA, R Experimental lung tumors in animals. 19~ Severi, L., editor.
    Tumors in Animala Perugia, Italy, Division of Cancer Research, Uni-
   versity of Perugia, June 1988. Pp. 59-84.
(54) KINOSITA, R., TAXAKA, T. Induction of lung carcinoma by 4nitroquino.
   line N-oxide. In: Severi, L, editor. Tumors ln Animals. Perugia, Italy,
   Division of Cancer Research, University of Perugia, June lQ66. Pp.
    7l7-128.

(55) KIBYU, S., KUBATSUIVE, M. Polycyclic amuatic hydrocarbons in the
   cigarette tar produd by human smoking. Gann; Japanese Journal
   of Cancer Research (Tokyo) 57 : 317-322, August 1968.
(56) 90~ J., FMIvK, P., TUBXEE, V. Abh%ngigkeit der karsenogene
  Wirkung der Tabakdestillate von Ein+rkungsart. Oncologia (Basel)
   12: 22-27,lQsQ.

(57) KualP, H. Tobacco alkaloids and their pyrolysis products in the smoke.
   In: Von Euler, U. S, Editor. Tobacco Alkaloids and Related Compounds.
   New York, MoaniRan, 1986. Pp. 37-61.

(58) Ko~a~s- M., KOHCHI, S., HOEIE, A. Oarcinogenesis in the esophagus.
   I. Penetration of benzo(a) pyrene and other hydrocarbons into the
  esophageal mucosa. Gann;, Japanese Journal of Cancer Research
   (Tokyo) 56 (2) : 177-187, April 1965.

(53) LACASSAGXE, A., Bw-HOI, N. P., ~JDELA, F., Lmrr-LAUY, D., CHALK,
   0. Activiti can&rog@ne d'Hydrocarbures Aromatlques polycycliques
   a norjau Fluoranthene. Acta; Unio Intematlonalis contra Cancrum
   (Louvain) IQ : 4Q&4m 1983.

(60) LABNITZKI, I. F&ct of dgaretta smoke condensate on human foetal lung.
  British Empire Caner Campaign for Research. Atmual Report. 43:
   35o,lQ65.

(62) LA~AB, P., CHOUIUXUHKOV, I., LIXBEBMANN, C., GIJE~IN. M. Benw(a)
   pyrene content and carcinogenicity of cigarette smoke condensate.
   Journal of the National Cancer Institute (Washington) 37(5) : 573-579,
    November 1966.

(63) LEUCHTERBEBOEB, C., LEU~E+~~~BEBOEB, R. The role of influenza virus in
   the development of malignant transformation $n Vitro and in the res-
   piratory tmct of mice, with and without exposure to cigarette smoke.
   Rivista dl Biologia (Pemgia) 6Q : 445-483.1988.
(f33) LEUCHTENBEIWEB, C., LBUOHTERBQIOEB, R., HOBISBQWEB, M. Change of fre-
   quency and of spectmm of tumors in Snell's mice after chronic inhala-
   tion of fresh intermittent dgarette smoke. Proceedings of the America?
   Association for Cancer Research (Chicago) 8 : 4O,lQ67.
(64) LIPP, G., Do-=, U. Ueber die Anwendung der Dimethylphenole au
   Bestimmung von Nitrat und Nitrlt im Tabak. Beitrllge zur Tabakfor-
   schung. (Hamburg) 2 : 345459.1964.
(65) Lm, J. B., tim, E. P.. Ja. Polonium-210 in bronchial eDithelium of

  cigarette smokers. Science (Washington) 155: 6Q6, Feb. -3, 1967.
(66) LLTTLE, J. B., RaDposD, E. P., McCMms, H. L., HUNT, V. R. Distribution of
   Polonium-210 in pulmonary tissues of cigarette smokers. New England
   Journal of Medicine (Boston) 273(25) : 1343-1351, Dec. 16, 1965.
(67) Lm, J. B., RAD~, E. P., Ja, MCCOMZS, H. L.. HUXT, V. R. Polonium-
   210 in lungs and soft tissues of cigarette smokers. Radiation Research
   (New York) 22 : 209+, 1984.

164


(68) MAEBDEN, E. Some aspects of the relationship of radioactivity to lung
   cancer. New Zealand Medical Journal (Wellington) 64 : 367-376, July 19,
    1985.

(89) MOOBE, C. Smoking and cancer of the mouth, pharynx, and larynx.
   Journal of the American Medical Association (Chicago) lQl(4) : 107-110,
   Jan. 25.1985.

(70) MOBI, K. Enhancement of experimental lung cancer in mice by inhalation
   of cigarette smoke. Gann; the Japanese Journal of Cancer Research
   (Tokyo) 57 : 537-541, October 1968:

(71) NEU~ATE, G., DRUNKER, M., GEBVE, J., L~~TTICH, W., WICHEBN, H. Unter-
   suchung der fliichtigen Bm des Tabakrauches. Beitrilge zur Tabak-
   forschung (Hamburg) 3: 563-56Q,lQ66.

(72) NEUB~TH, G., PIBMANN, B., Liirrroxz, W., WI~IIEB~, H. Zur Frage der
   N-Nitros&Verbindungen im Tabakrauch II. Beitrllge Tabakforschung
   (Hamburg) 3 : 251-262,lQ65.

(78) NEWATE, G., PIBMANN, B., WICHEBH, H. Zur Frage der N-Nitroso-Ver-
   bindungen in Tabakrauch. BeitriIge Tabakforschung 2: 311319, 1984.
(74) NOAKE~, D. N. The carcinogeniclty of insecticide-sprayed tobacco in mice.
   Food and Cosmetics Toxicology (Oxford) 3 : 305-310, August 1985.
(75) NOBMAN, V., m, C. H. Nitrogen oxides in tobacco smoke. N&me
    (London) 205(4974) : 915-916, Feb. 27, 1965.
(76) R.ADFOED, E. P., JB., HUNT, V. R. Polonium-210 : a volatile radioelement
   in cigarettes. Science (Washington) 143: 247-249, Jan. 1'7, 1984.
(77) RADFOBD, E. P., JR, HUNT, V. R. Cigarettes and polonium-210. Science
   (Washington) 144 : 366-367, Apr. 24, 1964.
(78) REEVES, A. L., DEITCH, D., VOBW~LO, A. J. Berylium carcinogenesis I.
   Inhalation exposure of rats to beryllium sulfate aerosol. Cancer Re-
    search (Baltimore) 27(3) : 439-445, March 1967.
(79) ROCKEY, E. E. Evolution of cigarette smoking technics in dogs. Inter-
   national Surgery (Chicago) 46(5) : 404413, November 1968.
(80) ROCKEY, E. E., BP=& F. D. The ill effects of cigarette smoking in dogs.
   International Surgery (Chicago) 46(6) : 520-530, wmber 1968.
(81) SAFFI~TI, U., MONTE~ANO, R., SELLAKUMA& A. R., BOBO, S. A. Expcri-
   mental cancer of the lung. Inhibition by vitamin A of the lnductlon of
   tracheobronchial squamous metaplasia and squamous cell tumors. Can-
   cer (Philadelphia) 20( 5) : 857-864, May 1967.
(8.2) SCHMHHL, D. Vergleich der I&np5ndlichkeit zwischen Ratte und Mans
   gegen die carcinogene Wirkung von Tabakrauch Kondensaten. Arznei-
   mittel-Forschung (Aulendorf) 17 : 405, 1967.
(83) QCHMHHL, D., THOMAS, C. Vergleichene Priifung von Tabakrauchkonden-
   saten bei subcutaner und ovaler Applikation auf carcinogene wirkung
   bei Ratten. Zeitschrift f6r Krebsforschung (Berlin) 66: 291-296, 1984.
(84) SEELKOPF, C., RICKEN, W., DHOM, G. Untersuchunger Uber die krebser-
   zeugendeu Eigenschaften des Zigarettenbeeres. Zeitschrift fiir Krebs-
   forschung (Berlin) 65: 241-249, 1963.

(85) SELIKOFF, I. J., HAMMOND, E. C., CHUFF, J. Asbestos exposure, smoking
   and neoplasia. Presented at the Sections on Diseases of the Chest and
    Preventive Medicine of the American Medical Association and the Amer-
   ican College of Chest Physicians, Atlantic City, N.J. June 19, 1967.
    [Unpublished]

(86) SE~FOTTEIN, W. J., HUBTZB, P. Nltrosamines as environmental carcino-
  gens. II. Evidence f?r the presence of nitrosamines in tobacco smoke
   condensate. Cancer Research (Chicago) 26 (pt. I) : 575579, April 1966.

165


(87)

(88)

(89)

(90)

(91)



(WI



(93)

(94)

(95)

(96)

(97)

(98)

(99)



(100)

(201)

(102)



(103)

SEVEBI, IA., editor. Lung Tumors in Animais. Perugia, Itaiy, Division of
Cancer Research, University of Perugia, June 1938. 970 PP.
SHMAD, L. M. Experimental cancer of the lungs. Federation Proceedings:
Translation Supplement (Bethesda) 22 (No. 2, pt. II) : 331-338, March-
April 1963.
STASSEWSKI, J. Smoking and cancer of the urinary bladder in males in
Poland. British Journal of Cancer (London) 20: 32-35, March 1988.
SUITDICBMAN, F. W., Dor?rm~~~, A. J. Studies of nickel carcinogenesis me-
tar&a&sing puimonary tumors in rats induced by the inhalation of nickel
carbonyl. American Journal of Pathology (New York) 46(6) : 1027-
1041, June 1985.
TIPTON, D. L, CBOCKEB, T. T. Duration of bronchial squamous metaplaaia
produced in dogs by cigarette smoke condensate. Journal of the National
Cancer Institute (Washington) 33(3) : 487-495, September 1984.
Tso, T. C., HABLEY, N., ALEXAND- L. T. Radium-228 and polonium-!ZlO in
burley and cigar wrapper tobacco. Tobacco (New York) 183(8) : 28-
29, Au& 19,liMi.
Tao, T. C., HAI&EY, N., A.IZXA~DE& L. T. Source of lead-210 and polonium-
210 in tobacco. Science (W!ashington) 153(3738) : 880-&E, Aug. lQ,lfJ88.
U.S. PUIILXC HE&LTH Ssavrou~ National Center for Health Stati~tlcs.
Mortality from diseases associated with smoking: United States, 1950-
84. Washington, U.S. I%partment of Health, Education, and Welfare,
Vital and Health Statistics Series 20, No. 4, Public Health Service Pub
lie&ion No. 1083, October 1988.45 pp.
U.S. PWLIC H~M.TE Smwrcxs. Smoking and Health. Report of the Advisory
Cohttee to the &rrgeon General of the Public Health Service. [Wash-
ington] U.S. Department of Health, Education, and WeRare+ 1984.
3f37 PP.
VAN DUUBEH, B. I*, Swag, A., SEQAL, A., Oases, L., LA~~SETH, L. The
tumor-promoting agenta of tobacco leai and tobacco smoke condensate.
Journal of the National Cancer Institute (Washington) 37(4) : 519-528,
Octcder 1938.
Vi&?KIwA, G. YA., CEEaKEs, L. D. Nucleic acid content in the liver of rats
fed on a diet of selenium. Voprosy Piteniia (Moskva) 25(l) : 3033,
1988.

WAQO~EX, J. E., Aaouna, V. 1, LUNDIN, F. E., JB, HOLADAY, D. A., LUIYD,
J. W. Radiation as the cause of lung cancer among uranium miners.
New England Journal of Medicine (Boston) 273(4) : 181-188. July 22,
1065.

WATSON, W. IA, FABPOUB, A. Terminal bronchiolar or "Alveolar Cell"
cancer of the lung. 286 cases. Cancer (Philadelphia) lD(6) : 778-780,
June 1988.
WEST, P. W. Selenium in paper and its ,possible association with lung
cancer among cigarette smokers. [Unpublished.] 7 pp.
WESWIO, P. H., YAMAM~T~, R. S., FALK, H., TWSLEP, I. J., u J. R.,
BONE, J. F. Bioassay of selenium compounds for carcinogenesis in rats.
Corvaiiia, Oreg. Oregon State University, Department of Agricuiturai
Chemistry and Veterinary Medicine, 1988. Contract No. PH 43-GM4.
WICKEIV, A. J. Environmental and personal factors in lung cancer and
bronchitis mortaiity in Northern Ireland, lfKiO+2 (London, England)
Tobacco Research Council, Research Paper No. 9,19&X 84 pp.
WOBLD HEALTH Oao~ivm~rxo~. Expert Cqmmittee on Cancer. Histo-
pathology of Lung Tumours. First Report of Expert Committee on
Cancer, Oslo, Nov. 17-22,1958. WHO/CANC/z Rev. 1 Feb. 23.1i359.14 pp.

166


(104) WYNDEB, E. L., Bzao, J. W. Lung cancer among nonsmokers. With special
   reference to histological patterns. (In press.) Cancer (Philadelphia) :
    July 16, 1966.
(105) WYNDER, E. L.. GMHAM, E. H. Tobacco smoking as a possible etiologic
    factor in bronchogenic carcinoma. A study of 684-proved cases. Journal
    of the American Medical Association (Chicago) 143: 323-336, l!%O.
(106) WYNDFB, E. L., HOFFMANN, D. The carcinogenicity of benzofluoranthenes.
   Cancer. (Philadelphia) l2(6) : 1194-1199, November-December 1953.
(107) WYNDER, E. L., HOFFMANN, D. Experimental tobacco cardnogenesis. Ad-
   vances in Cancer Research (New York) 8:24%453, 1964.
(108) WYNDE~, E. L., H~FFYANN, D. Reduction of tumorigenicity of cigarette
    smoke: An experimental approach. Journal of the American Medical
   Association (Chicago) 192(2) : 88-94, Apr. l2,1@65.
(109) WYNDE~, E. L., HOFFXARN, D. Tobacco and tobacco smoke. Studies in ex-
    perimental carcinogenesis. (In press.) New York, Academic Press, 1067.
(110) WYNDJLX, E. L., TAGUCHI, K., BADE, V., HOFFMAIVN, D. The eff& of
   cigarette smoke on respiratory tract of mice after passive inhaiation.
    (In press.) Cancer Research (Philadelphia) : 1967.
(111) WYNDEB, E. L., TAOUCHI, K., BADJIX, V. HOFFMANN, D. Effect of pazsive
    inhalation of cigarette smoke on the respiratory tract of mice and
    hamsters. Proceedings of the American Association for Cancer Research
    (Chicago) 7 : 77, April 1966.
(f12) YAVIN, A. I., DzP~~uacr, G., BAB~N, P. Polonium in dgarettegspectro-
    scopic analysis. Nature (London) 265(4974) : 89Q-900, Feb. 27, 1965.

       SUPPLEMENTAL REFERENCES

Sl. ABELIN, T. Smoking habits and survival of lung cancer patients. Applica-
    tion of the temporary expection of life as a measure of survival. Ameri-
    can Journal of Epidemiology (Baltimore) 84( 1) : 110-119, July 1966.
52. Ammri, T., TOKUHATA, G .K. Maternal age at birth and susceptibiiity to
   lung cancer. Lancet (London) 2: 1121-1123, Nov. 27, 1965.
53. AHAUZD, N. Geographical incidence of oesophageal cancer in West Kenya.
    East African Medical Journal (Nairobi) 43 (7) : 235248, July 1966.
S4. ALBERT, R E., LI~PMANN, M., SPIEGELMAN, J., L~ZZI, A., Nmaorv, N. The
    deposition and clearance of radioactive particles in the human lung.
    Archives of Environmental Health (Chicago) 14 : 16-15, January 1967.
SS. ALEXANDBOV, K., RAITCHEV, R. Contribution experimentale a la can&o-
    genese due au tabac. Doklady Bogarskoi Akademie Nauk (Soiiia)
    18(8) : 785-788,1985.
S6. ALFBED, L. J. Differential inhibition of deoxyribonucleic acid and ribo-
    nucleic acid synthesis induced in cultured mammalian cells by 7: 12
   dimethylbenzanthracene. Nature (London) 268: 1339-1341, December
     1965.
57. AND&BOON, A. E., JR, HEUNANDEZ, J. A., HOLMES, W. L., Foa~xczn, A. 0.
   Pulmonary emphysema. Prevalence, severity, and anatomical patterns in
    macrosections, with respect to smoking habits. Archives of Environ-
    mental Health (Chicago) l2(5) : 568-577, May 1966.
58. Bawza, G. M., NOVOTNY, J. The formation of aromatic hydrocarbons at
   high temperatures. XVIII. The pyrolysis of n&cane. Australian
   Journal of Chemistry (East Melbourne) 16(4) : 613-622, August 1363.
SQ. BALL, J. K., McCq J. A., SMITH, M. F. The interaction in sitro of
   polycyclic aromatic hydrocarbons with deoxyribonucleic acids. Bie
   chemica et Biophysics Acta (Amsterdam) 103 : 275-285, June 8.1065.



    271-394 0 - 6, - 12                            167


810. Brnn~~& J. R., l&arm, hf., S~rr~mzs, D. W. Survival in 6086 cases or
   bronchial carcinoma. Lancet (London) l(74QQ) : 1067-1070, May 20,
    1967.

Sll. Boxsee, Q. M., CLAYSO~, D. B., JULL, J. W. Some aspeots of the experi-
   mental induction of tumours of the bladder. British Medical Bulletin
    (London) 14(2) : 146-152, May 1958.
512. BOYD, J., LANOMAN, M., DOLL, R. The epidemiology of gastrointestinal can-
   cer with special reference to causation. Cut (London) 5 : X%-20& April
    1964.

913. BOYLAND, E., Rou, F. J. C., GOBBOD, J. W. Induction of pulmonary tumonrs
   in mice by nitrosonornicotine, a possible constituent of tobacco smoke.
   Nature (London) 202 : 1128, June 13,1Q64.
514. Bansrow, L. Epidemiology of cancer. World-Wide Abstracts of General
    Medicine (New York) 4 : 34-36, October 1961.
516. BBEBLOW, L. Cancer epidemiology-implications for control. American
   Journal of Public Health and the Nation's Health (New York) 53(2) :
   218-222, February 1983.

516. BBEBLOW, L. Environmental carcinogenesis. California Medicine (San
    Francisco) 101(5) : 371-375, November 1964.
917. Bansrow, L. Coals for Callfomla against cancer. California Medicine (San
    Francisco) 104(4) : 254-266A, April 1966.
818. BIUIZEK, J., Rum, A. Changes of body weight in normal men who stop
   smoking cigarettes. Science ( Washlngton) 125 : 1206, June 14, 1957.
519. B~~LL, P., Dunn, J. E. JR, Bnss~ow. L. The occupational-social Class risks
    of cancer mortality in men. Journal of Chronic Diseases (St. Louis)
    12 (6) : 600-621, December 1960.

S26. Ca~sou, S., GOLDIIAM~~, R., WFJNBEBO, M. 5. Characterization of physical,
       _. . I -_    -~_ .- ._            . -I_. _-
   chemical, and biological pr&rties'df mucus in the inta& animal. Annals
   of the New York Academy of Sciences 130 (Article 3) : 935-943, Sepb?m-
    her-October 1960.

521. Casson, S., GOLDIIAMEB, R., Cm TEB, R. Responses of ciliated epithelium
   to irritants. Mucus transport in the respiratory tract. American Review
   of Respiratory Diseases (Baltimore) 93 : 86-92, March 1988.
922. CASTIOLIANO, S. G. Rebuttal to "1,CJOQ cases of lung cancer" (letter). Penn-
   sylvania Medical Journal (Harrisburg) 67(8) : 94, Q6, 1984.
923. CEDEBLoF, R., Fmnnao. L., Jonason, E., RAW, L. Morbidity among mono-
   zygotic twins. Archives of Environmental Health (Chicago) 10 : 346-350,
    February 1985.

824. Cigarettes, cancer and Polonium-210. Medical Journal of Australia (Syd-
   ney) 1: 548-549, Mar. 26.1968.

525. ~OBIUQELD, J., HAEIVS~L, W., HAMYOND, E. C., LILIE~ELD, A. M., SHIY-
   KIN, M. B., WYNDEB, E. L. Smoking and lung cancer: Recent evidence
    and a disccussion of some questions. Journal of the National Cancer
    Institute (Washington) 22 (I) : 173-203, January 1959.
526. COUBY, C., PAILLAS, J., RUFFIXO, J., MABIE, M., FB~TTIEZ, J. Association
    d'un cancer bronchique et d'un cancer du plancher buccal. Semaine des
    Hopitaux de Paris 43( 8/2) : 530-533, February 1967.
527. DALEAMN, T., RYLANDEB, B.. Cigarette smoke and ciliastasis. EKect of
   varying composition of smoke. Archives of Environmental Health (Chi-
   cago) 13( 1) : 47-50, July 1968.

928. DAMON, A., MCCLTJN~, J. P. Previous pulmonary disease and lung cancer:
    A case-control study. Journal of Chronic Diseases (St. Louis) 20 :
    5Q-63,1967.

168


529. DABK, J., PEMB~BT~N, M., O'CONNOR, M., RUSSELL, M. H. Relighting of
   cigarettes and lung cancer. British Medical Journal (London) 2(6366) :
   1164-1166, Nov. 9, 1963.

536. DUN, G. Lung cancer among white South Africans. British Medical
    Journal (London) 2 : 120-121, July 14, lQ62.
S31. DEZAN, 0. Lung cancer in the Channel Islands. British Journal of Cancer
    (London) 19 : 661-686, 1965.

532. DEAN, G. Lung cancer and bronchitis in Northern Ireland, 1980-82. British
   Medical Journal (London) 1: 1606-1614, June 1868.
533. DEL~BUE, N. C. Reconsideration of some significant aspects of the cigarette
   smoking-lung cancer controversy. Canadian Medical Association Journal
    (Toronto) 89: 1277-1283, Dec. 21, 1963.
534. DE MAEY~~-GUI~NA~D, J., DE M-ma, 1. EKect of carcinogenic and non-
    carcinogenic hydrocarbons on interferon synthesis and virus plaque
   development. Journal of the National Cancer Institute (Washington)
   34(2) : 265-272, February 1965.

S35. DIPAOL~, J. A,, K~TIIV, P. Teratogenesis~ncogenesis: A study of possible
   relationships. Archives of Pathology (Chicago) 81: 3-23, January 1988.
536. DOLL, R. Interpretations of epidemiologic data. Cancer Research (Chicago)
   23(10) : 1613-1623, November 1983.

537. DOLL, R. Epidemiologlcal observations on susceptibility to cancer in man
    with special reference to age. Acta ; Unio Internationalis contra Cancrum
   (Louvain) 26 : 747-752, lQ64.

538. DOLL, R. Cancer: The possibilities. British Medical Journal (London)
    l( 5433) : 471-473, Feb. 20, 1985.

539. DOLL, R. Cancer bronchique et tabac. Branches (Paris) 16(6) : 313-324.
   September-October 1986.

S40. DONTENWILL, .W. Experimentelle Untersuchungen sur Genese des Lungen-
   carcinoms. Arsneimittel-Forschung ( Aulendorf) 14 : 774-780, Juiy 1984.
541. D~NTENWILL, W., RE~KZEH, G., STADLEB, L. Inhalationsexperimente lm
   Zigarettenrauch. Beitrllge sur Tabakforschung (Hamburg) 3 : 438448,
    1966.

542. DB~~ENDIJK, A. C. Smoking and lung cancer. Triangle: Sandoe Journal
   of Medical Science (Basel) 7 : 166-169, 1966.
543. DUNN, J. E., JR, WEIB, J. M. Cancer experience of several occupational
   groups followed prospectively. American Journal of Public Health and
   the Nation's Health (New York) 65(Q) : 1367-1375, September 1965.
S44. DUGAN-REYNALS, F. Studies on the combined eKects of fowl pox vim8 and
    methylcholanthrene in chickens. Annals of the New York Academy of
    Sciences 64 : 977-QQl,lQ!X.

545. ELMENHOBST, H., RECKZEH, G. Aromatische Kohlenwasserstoife im Tabak-
   rauch. Beitrllge sur Tabakforschung (Hamburg) 2 (6) : 18%264, May
    1964.

546. The endocrine and genetic factors in cancer of the lung. Growth (Phila-
   delphia) 28( 1) : l-16, March 1964.

547. EPSTEIN, 5. 5. Two sensitive tests for carcinogens in the air. Journal of the
   Air Pollution Control Association (Pittsburgh) 16 (10) : 546-661, October
    1986.

548. EPSTEIN, S. S., JOSHI, S., ANDBEA, J., MANTEL, N., SAWICKI, E., STANLEY, T.,
   TA~OB, E. C. Carcinogenicity of organic particulate pollutants in urban
   air after administration of trace quantities to neonatal mice. Nature
   (London) 212(6068) : 1365-1307, Dec. 17,1Q66.

169


S49. EPST~N, S. 9.. Sv M., FALK, H. L., MANTEL, N. On the association be-
   tween photodynamic and carcinogenic activities in polycyclic compouuds.
   Cancer Research (Chicago) 24 : -2, June 1964.
550. Fzam, E. S., BA~ATTA, 1. J.  Polonium-210 in tobacco, cigarette smoke, and
   selected human organs. Public Health Reports (Washington) 81(2) :
   121-327, February 1968.

Sal. FIN~E~LAND, A, KOPECNY, J. Rakovina Plic U Zen. Sbornik Vedeckych
   Prad Lekarske Fakulty Karlovy University (Hradec Kralove) 8(4) :
    4964QQ, lQ66.

852. Wsnnzm, M. Russian ideas on lung cancer. Postgraduate Medicine ( Minne
   apolis) 36 (8) : 55Q-560, May lQ64,

563. FLAK& A. Toxicity of whole tobacco tar. Nature (London) 204 (4958) :
    592493, Nov. 7.1964.

864. FLAYAI~T, R., Lasemuq O., LAMAR, P., LEBUEBIHAIE, J., DENOIX, P. DiKer-
   erences in sex ratio according to cancer site and possible relationship
    with use of tobacco and alcohoL Review of 66,660 cases. Journal of the
   National Cancer Institute (Washington) 32(6) : 1809-1316, June 1964.
S55. Future mortality from carcinoma of the lung (comments and abstracts).
   Medical Journal of Australia (Sydney) 2 : Q4Q-Q60, Nov. l2,1Q66.
866. Gains and losses reported in the war on cancer. Medical World News (New
    York) 8 (1) : 32, Jan. 6,1Q67.

SS?. Gore, H. H. Cigarette smoking and cancer of the bladder (letter). Virginia
   Medical Monthly (Richmond) 93 : 227, April 1968.
868. G~AEAM, S. Cross-cultural distributions of cancer : Implications for socio-
   logical epidemiology. Presented at the Annual Meeting of the American
   Sociological Association, Miami, Fla., Sept. 29, 1986. CUnpublished.1
569. Gaon, K. Zur Gaschromatographie des Cigarettenrauches. Beitrlige zur
   Tabakforschung (Hamburg) 3 (6) : u, September 1968.
860. GSELL, V. O., June, C. Lungenkrebs in alpinem lllndlichem Gebiet ohne
   Luftverschmutzung. Deutsche Msdizinische Wochenschrlft ( Stuttgart)
    8Q(lO) : QOQ-QlQ,lQ64.

S61. H~mvszm, W. Farther epidemiological tests of theories on lung cancer
   etiology. Public Health Reports (Washington) 71: 163-172, 1953.
S62. HAMMOND, E. C. Smoking in relation to mortality and morbidity. Findings
   in flrst 34 months of followup in prospective study started in 1969.
    Journal of the National Cancer Institute (Washington) 32(6) : 1161-
    1188, May 1964.

563. HANBUBY, W. J. Bronchogenic carcinoma in women. Thorax (London) 19:
    338-342, July 1964.

564. HECKEB, E. Di Cocarcinogene des Crotoniils. In: Doerr, W., Linder, F..
    Wagner, G., editors. Aktuelle Probleme aus dem Gebiet der Cancerologle.
    New York, Springer 1966. Pp. 121-127.
S66. HECK=, E., Kunrnvx, H. Ueber die WirkstoKe des Croton6ls IV. Reindars-
    t&lung und Charakterisierung der entzilndlichen und cocarcinogenen
   WirkstoKe BI und BI. Zeitschrift fiir Krebsforschung (Berlin) 67: 176,
    1966.

966. HEISE, E., GOBLICE, M. Growth and therapy of mammary tumours in-
    duced by 7,12dimethylbenzantracene in rats. British Journal of Cancer
    (London) 20 : 639645, September 1968.
967. HI~~INSON, J. Etiological factors in gastrointestinal cancer in man. Jour-
   nal of the National Cancer Institute (Washington) 37(4) : 527-t%
    October 1966.

568. HILL, C. R. Polonium-210 in man. Nature (London ) 268(5OOQ) : 423-438,
    Cctober 30.1985.

170


.S@. HOFFMANN, D., RATEKAMP, G., AND WYNDEB, E L. Comparison of the yields
   of several selected components in the smoke from diKerent tobacco
   products. Journal of the National Cancer Institute (~Washington) 31:
   627637, September 1963.

S70. HOFFMANN, D., RUBIN, J. Chemical studles on tobacco smoke. I. The
   quantitative determination of indoles in cigarette smoke. Beitrlige zur
   Tabakforschung (Hamburg) 3: -14, 1986.
571. HO~ANN, D., WYRD~ E. L Selective filtration of phenols from cigarette
   smoke. Journal of the National Cancer Institute (Washington) 36: 67-
   84, January 1983.

572. HOLUND, R. H., Acmmo, A. R Current status of arsenic in American
   cigarettes. Cancer (Philadelphia) 19: 1248-1256, September 1986.
573. HO-D, R. H., WILSON, R. H., Acmoo, A. R., MCCALL, M. S., Cm, D. A.,
   La~z, E C. The cigarette smoke-arsenic-cancer of the lung problem.
   A&a ; Unio Internationalis contra Cancrum (Louvain) 15: 608611,
    1959.

S74. HYDE, L. Cigarette smoking and cancer of the lung. Is there really any
   etiologic relationship? California Medicine (San Francisco) Q8(6) :
   315317, June 1983.

5'75. JELUNCK, P. H., IBWIN, L. EKect of carcinogenic polynudear hydrocarbons
   on the metabolism of estrogens. Nature (London) lQ6(4882) : 787-788,
   May 25,X%3.

576. ~YS, J. The smoking enigma. Canadian Journal of Public Health
    (Toronto) 56( 3) : 165-168, Mmh 1985.

577. Ka&z, A. Z., Tzzuus, M. The association of alcohol and tobacco with can-
   cer of the mouth and pharynx. American Journal of Public Health and
   the Nation% Health (New York) 55(10) : 1578-1586, October lQ6&
578. KELLY, M. G;, NEWTON, W. L, O'GAEA, R. W. Susceptibility of newborn
    germ-free mice to tumor induction by 3-methylcholanthrene. Cancer
   Research (Chicago) 23 (7) : 978-982, August 1983.
S79. Kmwuz, C. J., BATTISTA, S. P. Components of cigarette smoke with dliary-
   depressant activity. New England Journal of Medicine (Boston)
   26Q(22) : 1161-1166, Nov. 28, 1983.

580. KILBUBN, K. H., S-MO, J. E., editors. Symposium on structure, function
   and measurement of respiratory cilia. American Review oi Respiratory
   Diseases 93 (No. 3, pt. 2) : 184, March 1968.
581. KISBEN, D. M. The significance of personality in lung cancer in men.
   Annals of the New York Academy of Sciences 125: 8Xl-826, 1968.
S82. ROTIN, P., FAX& H. L. Atmospheric factors in pathogenesis of lung cancer.
   Advances in Cancer Research (New York) 7 : 475-514, 1963.
883. K~TIN, P., WISPY, D. V. Production of lung cancer in mice by inhalation
   exposure to influensa virus and aerosols of hydrocarbons. Progress in
   Experimental Tumor Research (Basel) 3: 186215, 1983.
Sf+%. KOURILBKY, R.. HAPPEBT, J. L. Pathologie cornpaw% du cancer bronchopul-
   monaire chez l'homme et chez la souris cam&i& par le goudron de la
   fumed de cigarette. Bulletin de 1'Academie Nationale de Medicine
    (Paris) 148 : 346-356, 1964.

$85 KOURILBKY, R., HAPPE~T, J. L. Comparaison morphologlque, radiologique et
   blologique entre le cancer pulmonaire provoque chez la souris par le
   goudron de funi& de cigarettes et le cancer bronchopulmonaire spontan6
   chez l'homme. Journal Francais de Medicine et ChlNrgie Thoraciques
   (Paris) 19: 467-478, May-June 1985.

171


886. Rasrnmm, H. J. Empirical relationship of lung cancer incidence to cigarette
    smoking and a stochastic model for the mode action of carcinogens.
    Biometrics (Tucson) 21: 83Q-857, Decembsr 1986.
887. La controverse sur le cancer bronchique (editorial). Medicine et Hygiene
    (Geneva) 25 (769) : 216-217, March 1987.
S88. LANQMAN, M. J. S., DOLL, R., WILBON, J. Plasma and salivary tbiocyanate
    in gastric cancer. Gut (London) 7 : 549-752, 1986.
889. LEA, A. J. Cigarette smoking and cancer of the lungs and of the bladder.
    Lancet (London) 1: 5QO-591, Mar. 12,X)66.
SQO. LEES, T. W. The fall of the lung-cancer wave. (Letter) Lancet (London) 2 :
    443, Aug. 28, 1985.

591. LEITCH, A., KENNAWAY, E. L. Experimental production of cancer by arse-
   nic. British Medical Journal (London) 2: 1107-1108, Dec. 9.1922.
592. LEUCHTENBEBGEB, C., LJZUCETENBEBGEB, R. Cytological aud cytochemical
   eKects of agents implicated in various pathological conditions: The
   eKect of viruses and of cigarette smoke on the cell and its neucleic
   adds. International Review of Cytology (New York) 14: 281-326,1983.
593. LEUCHTENBEWEB, C., LEUCHTENBE&OEB, R. Cytochemie der Krebsverander-
   ung. Schweizerisches Institut fur Experimentelle Krebsforschung
    (Lausanne) Q6(14) : 446-456,X966.

804. LEUCHTENBEBOEB, C., LEUCHTENBEBOEB, R. DNS in Tumoren und bie. Vims-
    infektionen Zellaktivitat und DNS-Gehalt van interphase karnen. In:
    Handbuch der Histochemie 3 (pt. 3). Stuttgart, Fischer, 1966. Pp. l-63.
595. LE~CETENBEBGEB. C., LEUCHTENBEB~ER, R Substances cancerigenes. Mede-
    tine et Hygiene (Geneve) 25( 764) : 8Q-86, Jan. 25,1Q67.
SQ6. LEUCHTENBEEOEB, C.. LEUCHTENBEBGEB, R., RUCH, F., TANAKA, K., TANAKA,
    T. Cytological and cytochemical alterations in the respiratory tract of
    mice after exposure to cigarette smoke influenza virus, and both. Can-
    cer Research (Chicago) 23( 4) : 565-665, May 1983.
897. LEVIN, M. L. Smoking and cancer. Retrospective studies and epidemiologi-
    cal evaluation. Journal of Chronic Diseases (St. Louis) 16 : 376-381,
    May 1983.

SQ8. LEMN, M. L., GB~HAM, S. Tables for temporal factors in the cigarette-
    lung cancer relationship. Presented at the American Public Health Asso-
    ciation meeting, New York City, Oct. 8, 1984. [Unpublished.] 26 pp.
SQQ. LIBEMTI, P. S. The eKects of smoking on the oral cavity. Dental Students'
    Magazine 44 : 73%746,770,772,774, June 1986.
9100. LILIENFELD, A. M. The epidemiologic method in cancer research (editorial).
   Journal of Chronic Disease `(St. Louis) 8(6) : 64Q-654, November lQ68.
8101. LILIENFELD, A. M. The relationship of bladder cancer to smoking. Ameri-
    can Journal of Public Health and the Nation's Health (New York)
    54( 11) : 1864-1875, November 1984.

9102. LOMBAUD, H. L. An epidemiological study in lung cancer. Cancer (Phila-
    delphia) 18 (10) : 1301-1319, October 1985.
5163. LONO, P. H. Smoking and lung cancer. Medical Times (Manhasset) 88(4) :
    512-514, April 1960.

9104. Lung cancer in Japan especially in relation to the smoking habits. Tokyo,
    Japan Lung Cancer Association, 1966.18 pp.
9105. MABKUSH, R. E., SOHAAF, W. E., Szmz~, D. G. The influence of the death
    certifier on the results of epidemiologic studies. Journal of the National
    Medical Association (New York) 59(2) : 105-113, March 1987.
9166. MAaTIN, H. Feedback (letter to the editor). CA-A Cancer Journal for
    Clinicians (New York) 16 : 138, May-June lQ66.

172


5167. Maam, F., MAEm?, M. Frequencies of alveolar cells in concentrated sputum
   specimens related to cytologic classes. Acta Cytologica (Baltimore)
    lO(6) : 362-367, September-October 1986.
S168. McC~uno, J. P. Previous pulmonary infection in lung cancer; a review.
    Journal of Chronic Diseases (St. Louis) 26 (1) : 66-77, January lQ67.
~109. i%.bINSMA, L. A. A lung cancer epidemic in Western-Europe ; poeaibllitlea of
   controlling the disease. 11 Oancro (Torino) lQ( 2) : 7Q-67, 1968.
Silo. MILLX& J. A., MILLEB, E. C. Natural and synthetic chemical carcinogens in
   the etiology of cancer. Oancer Research (Chicago) 25(8) : l2Q2-13Q4,
    September 1985.

sill. MOLD, J. D., Srxvxxa, R. K., MEANS, R. E., RATE, J. M. The parafiln hydro-
   carbons of tobacco ; not-mu& IgO-, and Ant&o-homologs. Biochemistry
    (Washington) 2 (3) : 605-810 May-June 1983.
Sii2. MONMOYEBY, P. O'B. Nucleolar studies. The Bulletin of Pathology 7(3) :
    66-67, March 1966.

5113. MONMOMEBY, P. OtB., REYNOLDS, R. C, CooIs, J. E. Nucleolar %rpa" in-
   duced by flying spot ultraviolet nuclear irradiation. American Journal of
    Pathology (New York) 49 (3) : 556-507, September 1968.
~114. Mooaq G. E., Baose, I., SH~MBEWXB, R., Bocx, F. G. Tar and nicotine
   retrieval from 56 brands of cigarettes. Cancer (Philadelphia) 20(3) :
    32&332, March 1987.

SH5. loux~rs, S. T. Personal communication.
S116. NEAL, J., RIQDON, R. H. Absorption and excretion of benspyrene when fed
   to mice. Texas Reports on Biology and Medicine (Galveston) 22(l) :
    156-164, spring 1964.

5117. NELSON, N. Formal discussion of: Ohemical and physical careinogena.
    Cancer Research (Chicago) 26 : 1314-1316, September 1965.
5118. OCHBNEZB, A. Health hazards from tobacco. In: Canter, P. D., editor. Trau-
    matic Medicine and Surgery for the Attorney. London, Butterw'orths;
    1965. Pp. 267-234.

5119. OCHENEE, A. Cancer of the lung: Recognition and management. Surgical
   Clinics of North America (Philadelphia) 46(6) : 141121425, December
    1966.

5120. OPPEBB, V. M. Roken, longanker en sterftekansconcurrentie. Nederlanda,
    Tijdschrift Voor Geneeskunde (Amsterdam) 108: 1574-18&o, 1964.
5121. OSHIMA, Y., ISHDZAKI, T., MIYAMOTO, T., w J., M~xrxo, S. A study
    of Toyko-Yokohama asthma among Japanese. American Review of Res-
    piratory Diseases (Baltimore) QQ(4) : 632-634, October lQ64.
Sl22. OMAN, S., BABSON, J. Hydrocarbons of cigar smoke. Tobacco (New York)
    169 (24) : 36-32, Dec. ll,lQ64.

5123. PAUL, J. EL, REPNOLDB, R. C., Monmo~mty, P. O'B. Inhibition of DNA-
    dependent RNA polymerase by 4Nitroqoinoline-N-Oxide in isolated
    nuclei. [Unpublished.] 4pp.

S124. PAULIN, R. Cigarette et cancer du poumon. Lava1 Medical (Quebec) 35 :
    72Q-732, June lQ64.

Sls. PHILLIPB, A. J. An analysis of the increase in lung cancer in Canada.
   Canadian Medical Association Journal (Toronto) 95: 1172-1174, Dec. 3,
    1966.

5126. PIKE, M. C., DOLL, R Age at onset of lung cancer: Significance in relation
    to effect of smoking. Lancet (London) 1: 66&66& Mar. 27,1Q65.
S127. Pomm& F. Rapporti epidemiologicic tra trumori primitivi de1 polmone,
   fumo dl sigarette e malattie pregresse dell' apperato respiratorio. Annali
   della Sanita Pubblica (Roma) 25: 1271-1288, November-December 1984.

173


5128. Pmoz, 0. A. Lung cancer and tobacco smoking. Medical World (London) :
    U-21, June l!klB.

5129. RAVENHOLT, R. T. Malignant cellular evolution. An analysis of the causa-
    tion and prevention of cancer. Lancet (London) 1: 523-526, Mar. 5,1233.
5130. Rxrzonos, R. C., MONTOOMEBY, P. O'B.. HUQHIUS, B. Nucleolar "caps" pro-
   duced by Actlnomycin D. Cancer Research (Chicago) 24(l) : l289-1217,
    August 1264.

Sl31. RIGDON, R. H., COBSBEN, G. Pulmonary lesions in the dogs from methylchol-
    anthrene. Archives of Pathology (Chicago) 75 : 323-331, March 1963.
8132. RIGDON, R. H., GIANNUKOB, N. J. Effect of carcinogenic hydrocarbons on
   growth of mice. Archives of Pathology (Chicago) 77 : .lOS-204, Wbruary
     1964.

5133. RIODON, R. H., Nq J. Absorption and excretion of benzpyrene observa-
    tions in the duck, chicken, mouse, and dog. Texas Reports on Biology and
    Medicine (Galveston) 2l(2) : 247-261, summer 1063.
5134. RIGIWN, R. H., NEAL, J. Effects of feeding Benzo(a)pyrene on fertility,
    embryos, and young mice. Journal of the National Cancer Institute
    (Washington) 34(2) : 297-305, February 1066.
5135. RIGD~N, R. H., NIC&, J. Effect of intratracheal injection of Benzo(a)pyrene
    on ducks. Texaa Reports of Biology and Medicine (Galveston) 23(2) :
    494508, summer 1935.

5136. ROBBWS, W. T. Bronchial epithelium in smoking and nonamoklng college
    students. Journal of the American College Health Association (Ithaca)
    14 : 26&266, April 1966.

S137. ROBEBTBOIP, L. ,S. Summary of a Report. The Eighth International Cancer
    Congress: Moscow, July 22-28, ,1!962. South African Cancer Bulletin
    (Johannesburg) 6(4) : 13%135, OctoberdDeeember, 1262.
5138. ROE, F. J. C., FED, W. E. H. Chronic toxicity of essential oils and certain
    other products of natural origin. Food and Cosmetic Toxicology (Ox-
    ford) 3 : 3l1324,1966.

5139. ROE, F. J. C., MITCHELY, B. C. V., W~~rxxa, M. Tests for carcinogenesis
    using newborn mice : 1.2 benzanthracene, &naphthylamine, 2-naphthyl-
    hydroxylamine and ethyl methane sulphonate. British Journal of Cancer
    (London) 17(2) : 25&260, June1263.

5140. R~SENBLATT, M. B. ,Sex distribution, longevity, smoking, and lung cancer.
    Journal of the American Geriatrics Society (Baltimore) 14(7) : 711-715,
    July X3&3.

8141. *Ross, J. C., LEY, G. D., KRTJMHOLZ, R A, 'RAHBAEI, H. A technique for
    evaluation of gas mixing in the lung: Studies in cigarette smokers and
    nonsmokers. American Review of Respiratory Diseases (Baltimore) 95:
    447453, March 1267.

8142 Rusfmnn, W. 0. Cytology for early diagnosis of lung cancer. (Current
    Opinion) Medical Tribune (New York) 4 : Nov. 15,X%3.
5143. Russz~.~, W. 0. Exfoliative cytology for early diagnosis of lung cancer.
    Postgraduate Medicine (Minneapolis) 37(4) : 848, 848, AM), April 1265.
9144. Rnssrx~, W. O., NEIDHA~J~, H. W., CHANQ, S. C. How relia.ble is exfolia-
    tive cytology as a diagnostic tool? ,Surgery (St. Louis) 54(5) : %X&832,
    November 1063.

5145. RUBBELL, W. 0.. NEIDHABDT, H. W., MOUXTADI, C L., GBIFFITE, K Bf.,
    (3aarp0, J. P. Cytodiagnosis of lung cancer. A report of a 4year labora-
   tory. clinical, and statistical study with a review of the literature on
   lung cancer and pulmonary cytology. Acta Cytologica (Baltimore) 7 (1) :
    l-44, January-February 1963.

174


~14. SAWICKI, E., STANLEY, T. W., HAUEEB, T. R., JOHNSON, H., E-T, W.
   Correlation of piperonal test values for aromatic compounds with the
   atmospheric concentration of benw(a)pyrene. International Journal of
   Air and Water Pollution (London) 7 : 57-70,1963.
5147. SAW= K. 0.. SAWYEB, R. B., LUBCHENCO, A. E., MCKINNOIV, D. A., HILL,
   K. A. Fatal primary cancer of the lung in a teenage smoker. Cancer
   (Philadelphia) 2Q( 3) : 45147, March lQ67.
S143. SCELW~BTZ, T., SCHMIDT, F., Paonn, El. Verusche sur tierpathogenen Wirk-
   samkeit des Tabakmosaik-Virus. Naturwissenschaften (Berlin) 5308) :
    485-486,1066.

Si49. SEIDMAN, H. Lung cancer among Jewish, Catholic, and Protestant males
   in New York City. Cancer (~Philadelphia) 19(Z) : 135-106, February
    1066.

~156. SEUKOFF, I. J., HAMMOND, E. C., Cztwao, J. Asbestos exposure, smoking and
   neoplasia. Presented at the Sections on Diseases of the Chest and Preven-
    tive Medicine of the American Medical association and the American
   College of Chest Physicians, Atlantic City, N.J., June 19, 1067. [Unpub-
    lished] 21 pp.

~151. S~TOB& C. Relationship <between cancer and senile changes in the lung.
   Electron microscopy study. Gerontologia (Basel) O(4) : !23Q-243,1064.
5152. SKoOa, F., h&MBONQ, D. J., Cinxs~rrn, J. D., HAMPEL, A. ET, BOCK, R. M6.
    Cytokinin activity: Localixation in transfer RNA preparations. Science
    (Washington) 154 : 1354-1356, Dec. 9,1066.
5153. Smoking and cancer of the bladder. British Medical Journal (London)
    Z(5463) : 661-662, Sept. X3,1065.

5154. Smoking and esophageal histology (editorial). Journal of the American
    Medical Association (Chicago) 196: 151, July 12, lQ65.
Sl55. Smoking and women (editorial). Scientiilc American (New York) 214(4) :
    48,lQm

S156. S~~BEMA~K, B.., HUNT, V. R. Distribution of Polonium-210 in mice follow{-
   inhalation of ,polonium-2lQtagged tobacco smoke. Archives of Env'
    mental Health (Chicago) 14 : 585-588, April lQ67.
S157. SPIKNOETT, V. H. The beginning of the end of the increase in mortality from
    carcinoma of the lung. Thorax (London) 21: l32-13S, 1066.'
S158. Statistics on cancer. CA. A. Cancer Journal for Clinicians (New York)
   177(l) : 3443, January-February 1067.

S159. STEVENS, K. M. Lung Cancer : an evolutionary approach. Australian Journal
    of Experimental Biology and Medical Science (Adelaide) 43 : 421-423,
    July 1965.

5160. STITNIMANKABN, T., ROSAEN, R. D. Carcinoma of the lung at the Siriraj
    Hospital, Bangkok. Cancer (Philadelphia) lS(4) : 510615, April 1965.
Slt31. TOKUHATA, G. K. Smoking habits in lung-cancer pro&ml families and
    comparable control families. Journal of the National Cancer Institute
    (Washington) 31(5) : 1X3-1171, November 1063.
S162. T~KUHATA, G. K. Familial factors in human lung cancer and smoking.
    American Journal of Public Health and the Nation's Health (New York)
    54(l) : 24-32, January 1964.

5163. TOTH, B., SHUBIK, P. Studies with malignant lymphomas: Possible inter-
    action problems between chemical and viral-inducing agents. Bethesda,
    National Cancer Institute Monograph No. 22, September 1968. Pp.
     313-323.

SI64. TsoonrY.4, K. The relation of occupation to cancer especially cancer of
    the lung. Cancer (Philadelphia) lS(2) : 136-144, February 1065.
5165. U.S. P~JBLIC HEALTH SEBVICE. National Center for Health Statistics. Mor-
    tality trends in the United States: 1954-83. Washington, U.S. Depart-

175


ment of Health, Education, and Welfare, Vital and Health Statistic
Series 20, No. 2, Pub&! Health Service Pubiication No. 1600~ June 1966.
67 PP.

S166. U.S. PUEUO HFUTH Saavrcn National Center for Health Statistics, Cigar-
    ette smoking and health characteristics, United States July 1964-June
   1965. Washington, U.S. Department of Health, F&cation, and Welfare,
   Vital and Health Statistics Series 10, No. 84, Public Health Service Pub-
    lication No. 1000, May 1967.64 pp.

5167. VAKIL, B. J., MULEKAB, A. 1. Studies with the maximal histamine test
    Gut (London) 7 : 864-871, August 1965.
S168. Validity of lung cancer. Mortality data (letter). Journal of the American
   Medical B%aociation (Chicago) 199(Q) : 674-675, February 1967.
8169. Van DWBEIT, B. L., L~uosxru, L., &UK, A, Oruus, L. The tumor-enhanc.
    ing principles of Ctwtbn t2pUwn L. II. A comparative study. Cancer Be.
    search (Chicago) 26 (p. 1) : 1729-1722, August 1966.
5170. Volp Eur.xx-C~icnm~~, H. Tobacco pyrolysis studies by means of time-of.
    6ight mass spectrometry. Swedish Cancer Society Yearbook 4: 56%
    609, n.d.

S171. WAHI, P. N., KAPU% V. I*, LUTE~A, U. K., SEIVASTAVA, M. C. Submucow
    fibrosis of the oral cavity ; 2. Studies on epidemiology. BuBetin of the
    World Health Organization (Qoneva) 26 (6) : 792-799, 1966.
9172. WALEEB, T. It., Kmmm, J. E. Ciiiaetatic components in the gas phase of
    cigarette smoke. Science (Washington) 162 (3741) : l248-l250, Sept. 9,
     1966.

5178. W~DEB. 1. L. An appraisal of the smoking-lung-cancer issue. New Eng-
    land Journal of Medicine (Boston) 264(24) : 1235-1240, June 15,196l.
9174. W~DEB, E. L. The epidemiology of cancer of the bronchus: Facts and
    suppositions. Transactions of the American Bronchoesophagological be-
    sociation (St. Louis) : U-22,1966.
5175. WYNDEIZ, E. L., F~rarmnn, E. P., JR The role of a history of persistent cough
    in the epidemiology of lung cancer. American Beview of Respiratory
    Diseases (Baltimore) 94(6) : 709-729, November 1968.
5176. WYNDFZX, E. L., GOODMAN, D. A., HOFFMAN, D. Ciliatoxic componenta in
    cigarette smoke. II. Carboxyiic acids and aldehrdes. Cancer (Phii-
    adelphia) 18 : 506509, April 1966.

5177, WYNDEB, E. L, HOFFMANN, D. The role of skin neoplasia in tobacco carcino-
    genesis. In: James, G., Rosenthal, T., editors. Tobacco and Health.
    Springiield, Iii., C. C. Thomas, 1962. Pp. 61-71.
8178. WYNDEB, E. L., HOFFMANN, D. Experimental aspects of tobacco carcino-
    genesis. Diseases of the Cheat (Chicago) 44(4) : 887-844, October 1968.
8179. WYNDEB, E. L., HOFFMANN, D. Personal communication. March 1967.
9180. WYNDEB, E. L., HYAMS, L., SEI~EYATSU, T. Correlations of international
    cancer death rates. An epidemiological exercise. Cancer (Philadelphia)
    26 : 118-126, January 1967.

8181. WYNDEE, E. L., KAIBEB, H. E., C~OODMAN, D. A., HOFFMANN, D. A method for
    determining cilia&tic components in cigarette smoke. Cancer (Phil-
    adelphia) 16 : l!Z!-X325,1962.

9182. WYNDEE, E. L., KYET, J., DUNUI,, N., SEGI, M. An epidemiological investi-
    gation of gastric cancer. Cancer (Philadelphia) 16(11) : 1461-1496,
     November 1968.

Sl88. WYNDEE, E. L., MANTEL, N. Some epidemiological features of lung cancer
    among Jewish males. Cancer (Philadelphia) lQ(2) : 191-195, February
     1966.

176


5184. WYNDEB, E. L., TAOVCHI, K., BADEN, V., HOFFMANN, D. A study of tobacco
   carcinogenesis. Ix. The effect of passive inhalation of cigarette smoke on
    the respiratory tract of mice. Sloan-Kettering Institute for Cancer Re-
    search, New York, N.Y. Supported by Grant No. 231 from the American
    Cancer Society and in part by Grant No. CaO8747 from the National
   Cancer Institute. September 1988. [Unpublished.] 22 pp.
s185. ZAVOIQ, M. R. Crop chemicals and lung cancer (letter). Lancet (London)
    2 : 1072, Nov. 20,1986.

5186. ZILBEB, L. A., POSTNIKOVA, Z. A. Induction of a leukemogenic agent by a
    chemical carcinogen in inbred mice. Bethesda, National Cancer Institute
    Monograph No. 22, September 1986. Pp. 897-403.

177


CHAPTER 4

Other Conditions and Areas of Research

Smoking and PepticUlcer-.--..i---.--- __.____.___.__ -._-
Smoking and Disturbances ofVision------- _____ --_._--_.--
SmokingandCirrhosisof theLiver-.--------.----.--------
EffectsofSmokingDuringPregnancy ____ --.---___---___-__
Smoking and Accidents---- .__. -___----_-___---__--______
Psychosocial Aspects of Smoking----.-----.--------.---.--
References _____ --._-_.- _.___ - _.____ -.--_._---._--.._-__

P8W
181
183
184
185
187
188
193

179


SMOKING AND PEPTIC ULCER

Since the publication of the Surgeon General's 1964 Report, three of
the continuing prospective mortality * studies (&2?, 6',7) have provided
additional information which confirms the association between ciga-
rette smoking and mortality from peptic ulcer, especially gastric ulcer.
The mortality ratios increase with increases in amounts smoked. The
tables presented below illustrate the relationships involved. Although
Hammond's (6) study contained a large number of females, insutllcient
deaths from. peptic ulcer have occurred in cigarette smoking females
to provide statistically reliable data. A trend is observable among cigar
and/or pipe smokers with regard to increased mortality from gastric
ulcer, but the number of deaths is too small for significant conclusions
to be drawn.

TABLE 1 .-De&h ratea and m&a&y ratios for gastric and dum%naJ
  ULcers by specifi age group8 of ??lak l?igar& 8rnokem

                           Age 44-64           aessa-?o

           Site             Death mte  "%F  De8th ml43 ?sE'


Gastric ulcer . . . . . . . . . . . . . . ._..  `(2) 7     2.95   (7) 26      4.06
Duodenal ulcer ._.... . . .._.._..   (3) 7     2.86 --r
                              (21) 31      1.50

1 Number In parantheses indlcat8e death rate for psmor~ who never smoked cf&&y.
Sour.08: Hammond, E. C. [table U(s)].

TABLE 2.-M& death. rates and molzality ratio8 for gastric and duooTem.zl
ulcers by spec@ age groups for cuw& and ex-moker8 of cigarette8
&Y

                     Current olg8mtte allohm OnlJr       EX-dglUOtt.3
                                            wlokem only

        Site                  Death rate     2atP  "OmTtY

                                     76-84     m8n     (total)
                     sH4      44-74


Gastric ulcer ._.. . . . . . . . . 1 (2) 7   (5) 17   t-133    4.13      2.74
Duodenal ulcer .._.. . . . . .  (4) 8   (10) 29 (37) 122   2.98      2.13


1 Number In pamnthswa India&a death rata for pemona who have never amolted.
SOUBCI: U.S. veterana study [app. table A (7)).
o AU death rati thron@oUt tbln chapta IUO PcE lO&CiOD PoPulation, ~CSIl otherafae ftldia@d.

181


TABLE %-Peptic ulcer death rates by type and amount smoked, in ma.h 1

                 cige,rcttc cmokcn

Nonsmokers AU anoken                      - OZtlfe -
                                              COlOkraS
                 All                p1%rm
                       I-14
               SmOllllts      lb-24 2s+   smokiag

        P-----P--

0      13     13     2     18 19     12     12     10

' Includea &astric and/or duodenal ulmm
Sounm: Btudy of British physiclan8 (tabled 23 and 7.4 (a)].

A recent survey (I$), based on a national sample of 42,000 house-
hold interviews, shows that the prevalence of peptic ulcer is almost 100
percent greater in male cigarette smokers and over 50 percent higher
in females who smoke cigarettes as compared to those males and females
who ,had never smoked. Hammond's data (5) shows twice the number
of cigarette smokers reporting the occurrence of peptic ulcer over a
2-year follow-up period as contrasted to nonsmokers. This also in-
creases with increases in the amount smoked.

Several small retrospective clinical studies (4, 8, 59) have shown
significantly more smokers and less nonsmokers in their peptic ulcer
patients as compared to control groups. Doll (I) reviewed various pro-
spective studies on gastric ulcer therapy regimes, such as : die&bland,
normal, high and low fat; milk drips with alkali ; drugs; and advice
to stop smoking. The best results were obtained in patients who stop-
ped or cut down on their smoking habits. The Surgeon General's 1964
Report (14) points out the conflicting literature concerning the effects
of smoking on gastric secretion and motility. Lee (IO), in a small
series of peptic ulcer patients and controls, showed that after smoking,
74 percent of patients and 58 percent of controls had a significant rise
in free gastric acidit.y. Those subjects with initially normal or hyper-
acidity had the greatest response, whereas, of those with initial hypo-
acidity only 28 percent had an increase in gastric acidity. Five of nine
controls, smoking a non-nicotine cigarette preparation, also had a rise
in gastric acidity, perhaps due to factors in smoke other than nicotine
or to oral stimulation.

Cigarette smoking is shown to be associated with peptic ulcer.
This relationship is greater for gastric than duodenal ulcer and is
proportional to the amount smoked. The etiology of the peptic ulcer
diathesis is still unknown. Smoking is a definite risk factor in peptic
ulcer mortality. It may also be a factor in the delay in healing of a
gastric ulcer. More research is needed on the physiological effect of
smoking on the gastrointestinal tract.

182


SMOKING AND DISTURBANCES OF VISION

TOBACCO hBLYOPIA

Recent evidence points to the tobacco and/or alcohol amblyopias as
being manifestations of nutritional amblyopia (4, 7, 8, 17, %, $6).
Various deficiencies in factors of the vitamin B complex have been
implicated (4,6,7,10,17, %, 2%).

A new theory that chronic low vitamin B12 levels potentiate the
toxic effects of cyanide in tobacco has recently been expounded
(8,10, %,%9) .
The anatomical lesion in amblyopia seems to be a demyelinization
of the optic pathways, particularly in the papillomacular bundle
(10,17,%5).
In view of the fact that cyanide is neurotoxic, more research is needed
in this area to further elucidate its association with this disease entity.

OTHER DISEBSES

Several studies have hypothesized that Leber's optic atrophy, which
also is attributed to a demyelinization process in optic pathways, may
be associated with a defect in cyanide detoxification, which is aggra-
vated by the cyanide in tobacco smoke (I, 27).

VISUAL ACUXTY

The Surgeon General's 1964 Report, and others, cite evidence of
increased levels of carboxyhemoglobin in smokers (20, $244)) due to the
carbon monoxide content in tobacco smoke. It has been suggested that
a decrease in nighttime visual discrimination in smokers is related to
this increase in carboxyhemoglobin levels (9,15,16', 29). It may also
possibly be due to the relative anoxia produced by the carbon monoxide
inhalation from tobacco smoke. A value of only 5 percent carboxyhemo-
globin saturation, not uncommon in smokers, creates a physiological
state of anoxia equivalent to being at an elevation of 8,000 feet, with an
arterial 0, saturation of only 91 percent (X,16).

It is suggested that tobacco amblyopia is but a manifestation of
nutritional amblyopia, which is aggravated by tobacco smoking. More
research is needed on the toxicity of tobacco smoke, with special con-
cern for the cyanide component. Experiments have shown a visual
discrimination deficit, possibly related to the carbon monoxide content
of tobacco smoke. Burther work is needed in t.his area in order to
ascertain any clinical consequences.


SMOKING AND CIRRHOSIS OF THE LIVER

Increased mortality of smokers from cirrhosis of the liver is found
in the prospective studies. This has generally been thought to be largely
secondary to an association between smoking and heavy alcohol con-
sumption. Published data are inadequate to test this interpretation.
The three prospective studies (I, $7, 8, 4) ail show increased death
rates and mortality ratios from cirrhosis of the liver in cigarette
smokers.

TABLE l.-ikiortaiity ratios and d+m% rades for liver cirrhosis by sex
          and S$WC$C age gIVUp8

Mortality ratio-- _ ___ ___ ___ __ __     2.16
Death rate-- __________ _______   `(5) 10


' Calculated from ap . table 10 8.
' Numbers in parent ii esa Indicate death mtea for penom~ who have never amoked regalarly.
8OUECE: Hammond, E. C. [tables 2428, and apt. tabla 19 @I.

TABLE 2.-M&e morta&ity ratios and death rates for liver cirrhosis by
      age and amount smoked, in U.S. vetiana

I-

I O

Mortality ratio (total) __ _______   1.00
Death rate:
  Age45to54 ______________     9
  Age55to64 ______________     15
  Age65to74 ______________     16
  Age 75 to84 ______________     53

BOWCE: U.S. vetarms ahtdy [app. table A (d)].

Current emoken of cigarettes only

  2.72    3. 15


---- ----     7
   12     35
   74     57
---------------_

-


--









. -

21-29

3.61

   7
  44
  57
-------

ro+

5.50

162
46
87

__-____

Doll and Hill present their data with respect to cirrhosis of the
liver and alcoholism combined. See table 3.

TABLE 3.-M&e d&h rat+x for l&r cirrhosis by type and amount
         smoked, in B&h physicians

I I i
Non- AU C&I
smokera mnokexa mtt
           smok

           Number of cig.areti    Qlwn
        a
       ela 1-14 u-24 2Sf All wzk-
                      amolmta
---------

Clrrboein of liver and
alcnholfJm - - -_ - ---_ 1 0 11 12 S 842 IS 6 11 S
8OVIiCE: Study of British ph~lciam. [tablm 21 and 22 (I)].

184


The Surgeon General's 1964 Report points out the association be-
tmeen heavy smoking and excessive alcohol intake. In view of the
fact that "The increased death rate from cirrhosis among smokers
may reflect the consumption of alcohol and associated nutritional
deficiencies rather than the effect of cigarette smoking" (6), further
research is needed to elucidate the association between smoking and
cirrhosis of the liver.

EFFECTS OF SMOKING DURING PREGNANCY

The current new literature on pregnancy and smoking supports the
Surgeon General's findings that there are a greater number of "low
birth weight" babies and premature babies as defined by weight alone
(2,500 g.) in those women who smoke during their pregnancy (6,9,
18, $?0, 22, 94-96, $28, 3&B, 36). Furthermore, this decreased weight
has been shown to be consistent in each trimester (%!2,31,3g, 34,36)
and is proportional to the amount smoked during pregnancy (5,34-
@6,30--3~, 34,36).

There are many factors which affect the outcome of pregnancy.
These include con&itutional, pathobiological and psychological fac-
tors. Multiple-regression analyses of these various factors have shown
smoking to be a significant negative independent variable with respect
tobirthweight (1,5,l7).

Smoking has been linked to increased incidence of abortions and/or
stillbirths (6, 25, 26, 28? 30, 36), premature rupture of membrances
(30-32) and decreased male/female birth ratios (If, $25, M) ; however,
other studies do not support these findings (7, 9,24,3&B).

The significance of low birth weight and prematurity in regard to
increased fetal and infant mortality, has not been clearly demon-
strated. Most studies show no increased mortality (9, 31, 3B). How-
ever, Yerushalmy (34) and Underwood (32)) point out *hat although
the overa,ll mortality is the same between infants of smoking versus
nonsmoking mothers, premature babies (as defined by birth weight of
less than 2,500 g.) of smoking mothers have decreased mortality.
Other studies show a slight but significant increase in fetal mortality
for mothers who smoke (6, 8). MacMahon (19) shows that rather
than increasing the proportion of low birth weight babies, smoking
actually causes a shift t.o the left in the entire weight distribution
(fig. 1).
\Jansson (15) in his study states : "Thus, in the absence of other com-
plications, smoking mothers seem to make a proportionally greater
contrsbution to infants in *he weight group just below 2,500 g. where
the prognosis is Ibetter."

185


10

8

0

Non-smokers
Smokers

4 5 6 7 8 9   10 11

Fxomm l.-Percentage distribution by birth weight ' of infants of mothers who
did not smoke during pregnancy and of those who smoked one pack or more
per dw.

8ource : MacMahon, B., et al. [tlg. 1 (.I9) 1.

Steele (29) suggests that smoking is associated with sudden unex-
pected deaths in infancy. The relationship of smoking in mothers to
increased fetal morbidity, either perinatally or after long-term follow-
up, has not been adequately probed and is a major area for future
research.

Some studies show a relationship between smoking and decreased
gestational age (6, 8, 39) ; others do not (I, H).
Gestational age probably is a better indicator of fetal prematurity
than birth weight. Therefore, it may better reflect perinatal risk. Yet
even in the studies showing a correlation to smoking, this relationship
is less marked than that to birth weight (6,8, $$,3$). This may he
due to the relative di5culty in determining the last menstrual period
accurately and therefore the true gestational age.
The mechanisms by which smoking affects pregnancy have not been
elucidated. Events influencing decreased fetal ,birth weight have been
attributed to several factors :

1. Placental vasoconstriction due to nicotine. No direct evidence of
this exists at the present time. The e&&s of smoking on uterine blood
flow are being conducted in animal experimentation (S).

1 Birth weight (scale in pounds ; intervals of 4 ozs. )

186


2. Increased carbon monoxide in cord blood (Zle).
3. Decreased carbonic anhydrase activity due to increase in cord
carbon monoxide (m) .

4. A postulated direct effect on the fetus of some toxic agent in
cigarette smoke.

5. Decreased caloric intake due to decrease in appetite of smokers
(10). Several studies have shown t,hat there is no difference in weight
gain during pregnancy between smokers and nonsmokers (&?, 30,36).
Since smoking in general, does decrease appetite, it-might be well to
consider a difference in the type and/or distribution of caloric intake
between smokers and nonsmokers.

Kumar (17) has shown an increase in human uterine activity after
smoking, both in frequency and magnitude of contractions. However,
these findings were not observable in every patient. There was no
significant effect of nicotine on myometrial strips in WitTo from preg-
nant human uteri.

King and Becker (4,16) have done experimental work with nico-
t,ine on pregnant rats. High concentrations of nicotine had greater
toxic effects on pregnant rats than controls. The offspring were lighter
in weight and survived less well than controls.

Clearly, more research is needed to elucidate the significance of the
relationship of smoking in pregnancy and low birth weight. Addi-
tional long-range morbidity studies are needed, as well as studies on
the effect of smoking on uterine activity and placental blood flow.
Smoking does have an effect on the outcome of pregnancy. How-
ever, it is not known whether this effect is deleterious or not.
Until such evidence is presented so as to clearly define the role of
smoking in pregnancy, it is more prudent at this time to advise
pregnant women to stop or decrease their cigarette-smoking practices.

SMOKING AND ACCIDENTS

The most obvious contribution of smoking to accidents is as a cause
of fires. Estimates of the proportion of fire loss due to "smoking and
matches" (includes fires attributed to careless smoking and the care-
less use of matches and lighters by smokers ; does not include misuse
of matches by children) vary from 19 percent to 25 percent. The

187


National Fire Protection Association gives "smoking and matches" as
a reported cause of fire in various buildings for 1965 as follows (3) :

A~rtmente----_--__--------___-------------------------------------- 26
Boarding and rooming houses __________: ______________________________   30
Dormitories, etc----------_--____-___,_-__---__--___,----------------- 24
Dwellings (l- and S-family) __________-___--________________________--   19
Hospitals------_----_----_-______________________-------------------- 21
Hotels, seasonal-----_---__--___,____,-________,---------------------- 24
Hotels, year round---,----,--__---__,---_,-___,----------------------------- 35
Motels---------------,---_---__,--__--------------------------------------- 20
Nursing ________c_-_____________________________--------------------- 25

In 1965 there were 163,900 fires linked to smoking or the matches
used in smoking with a concomitant property loss of $80,400,000-in
1964, there were 159,460 fires and a property loss of $79,500,000 (6).
The statistics on the number of deaths attributed to those fires are
not available, but it is estimated that 1,800 people per year will die due
to fires caused by smoking and matches (7).

Smoking has been shown to cause decreased visual discrimination
especially under conditions of low illumination (4). This could have
serious implications with respect to night-time driving.
Several studies (I, 2, 6) have indicated an association between
smoking and traffic and industrial accidents, but the evidence is insuffi-
cient at this time to draw any significant conclusions. More research
is needed in this area.

PSYCHOSOCIAL ASPECTS OF SMOKING

There has been a sharp increase in the attention devoted to behav-
ioral research since the Surgeon General's 1964 Report. A number of
new concepts have been developed and more sophisticated multi-
variate approaches are being used. However, because of the recency
of these studies, very little in the way of findings has been published
on which firm conclusions may be based.

One of the byproducts of the Surgeon General's 1964 Report has
been its stimulation of more research in all areas of smoking, includ-
ing the psychosocial. Much research will soon be completed but has
not yet been reported in the literature.

Three behavioral science conferences have heen held since the Sur-
geon General's 1964 Report. The content of these conferences are either
in print (9) or mill shortly he published (11,21) . These conferences
dealt with many different studies and research findings, theories,
methodological criticisms, and discussions on a number of important
issues. Among the primary purposes was the development and speed-
ing up of communications among those doing work in the field, pre-

188


venting duplication and wasted effort, developing bettor measuring
instruments, and providing assistance in conceptualizing new theo-
retical models or further developing approaches already proposed.
Much prior research in the psychosocial aspects related to smoking,
while yielding valuable data and suggestive theory, has been concerned
largely with discrete variables or attributes and has looked for gross
differences between smokers and nonsmokers. Since it, is unlikely that
such research will discover that either group possesses an attribute
that is unique to it other than the behavior of smoking, the ability
of any single attribute to differentiate between these two populations
is bound to be limited. It is because of this that a number of investi-
gators have turned toward trying to distinguish subgroups of smokers,
as well as toward developing more unifying concepts. These efforts
are part of the attempt to obtain greater insight into the dynamics of
smoking and develop more powerful predictive instruments.

One area that shows conceptual and methodological maturation is
that of the study of smoking and personality. Much prior research
studied smoking in relation to such concepts as extroversion, intro-
version, neuroticism, emotional stability, orality, femininity, mascu-
linity, hypochondriasis, psychosomatic symptoms, risk taking and
chance orientation, psychopathic tendencies, achievement needs, social
approval, relationships to authority, independence, aggression, and
the like.

At the 1966 behavior research conference, it was pointed out that
a better understanding of the total personality structure must be
achieved in order to increase understanding of some of the psycho-
logical correlates of smoking. Factor and hierarchical models have
much to contribute to this approach (1). At the 1967 conference this
and other points pertinent to personality research related to smoking
were discussed, and a reminder of the utility of multivariate tech-
niques was repeated (20). Toward this end these investigators are now
studying university students, seeking factors in the realm of person-
ality integration such as experience of control, scope of awareness,
reality contact, self-insight, temporal perspective, independence, anxi-
ety, and the like. After these factors have been identified they can be
used as independent variables in testing hypotheses suggested by other
developments, such as the recently developed typology of smokers,
illustrating the potential yield from a cross-fertilization of unifying
concepts.

Theories which emphasize the role of anxiety in the development of
personality and in the understanding of personality dynamics (7) pro-
vide a unifying frame of reference which, when combined with an
understanding of the gratifications derived from smoking, may lead to
useful explanations and investigations into smoking behavior. They
may also provide some cohesiveness to research on such concepts as

189


guilt, self-punishment, need to fail, and risk-taking behavior as they
relate to the initiation, continuation, or inability to discontinue smok-
ing. Concepts from depth psychology, and ego psychology in par-
ticular may additionally illuminate the source and function of some
of the apparent inconsistencies among attitudes, beliefs, and behavior
noted by various investigators beyond that provided by dissonance
theory (6).

Another area showing some growth is represented by attempt to dis-
tinguish between the different levels of dosage to which smokers expose
themselves beyond that indicated by the average number of cigarettes
smoked daily. More sophisticated dosage measurements (18) obviously
have application in epidemiological research. They may also prove use-
ful in psychosocial research There is the possibility that an interplay
exists between the degree and kind of exposure, physiological and
psychological processes, and the dynamics, mechanisms, or degree of
difficulty involved in achieving long-term cessation of smoking.
Another conceptual development was contained in the proposal, re-
ported at the first of the national behavioral conferences (16) and later
refined (lb), of a new way to define smokers-in terms of the smoker's
use of cigarettes to help manage affect, i.e., emotions. From the types
of smoking identified (habitual smoking, smoking to increase positive
affect, to reduce negative affect, and psychologically addictive smok-
ing) and from a theoretical discussion of the dynamics involved in
their formation, possibilities'exist for the development, testing, and
application of t.heories and techniques for producing cessation either
in a clinic or a natural setting. By identifying differences between
smokers in the psychological use of cigarettes, the typology makes it
possible to develop theories and techniques to reinforce behavior
change and to expand knowledge of the dynamics of smoking behavior.
These concepts are undergoing empirical identification and veriflca-
tion at both the national level and in a variety of clinical settings
studying behavior change.

In one study (13)) for example, which compared three methods of
aid to people who were trying to give up smoking, efforts were made to
assess the subjects' progress, the nature of the change process, and the
social-psychological factors which influence the ability to give up
smoking and resist resuming. The investigators are analyzing their
data from the conceptual base of smoking types as well as from other
points of view in an examination of cessation processes.

The smoking typology is also being applied in an analysis of a survey
of adults' and adolescents' smoking habits and attitudes in the United
Kingdom (10). At the recent 1967 behavioral conference results were
presented showing the relation between these smoker types and nervous
irritation and relaxation smoking scales, wishing and trying to give Up
smoking, and addiction indicators for both adolescents and adults.

190


There were other kinds of analyses described in this research which
provide the stimulus for further development and testing of theory.
In this country, a parallel set of surveys has been going on which
utilized many questions from the above-mentioned survey just as that
survey also borrowed from it. Cross-cultural comparisons are thus
possible.
The national surveys of adult smoking behavior, beliefs, and at-
titudes in this country stimulated, and were also based upon, an
organizing framework which discussed some dimensions of a model
for behavior change (6). This framework incorporated the concepts
related to the typology of smokers previously mentioned and also
leaned heavily on a behavior model developed originally to provide
a theoretical base underlying participation in a mass X-ray screening
program (3,.& 16). Four dimensions of the framework are discussed
and postulated as being essential in considering whether smoking be-
havior change will or will not take place. They are : The motivations
for change (e.g., the exemplar role, economics, esthetics, mastery, and
others beside the health threat) ; the perception of the threat (e.g., the
awareness of the threat, the acceptance of the importance of the threat,
the personal relevance of the threat, and beliefs about the susceptibility
of the threat to intervention) ; the reasons for smoking in terms of
affect management, and the potential development and use of alterna-
tive psychological mechanisms ; and factors supporting or inhibiting
continuing reinforcement (e.g., the role of social forces, interpersonal
influences, the mass media, the behavior and attitudes of certain key
groups, and the general level of acceptability of the behavior).
Backed by the longitudinal data at the national level and subject
to multivariate analysis, this conceptual framework can potentially
be developed to the point whereby the parts may be related quan-
titatively and qualitatively to each other and thus afford a more
dynamic interpretation of the behavior change under consideration.
The possibility also exists for the development of an instrument for
the prediction of change, as well as an opportunity for the verification
of some prospective findings reported earlier (I.$). These constructs
have also since been extended to considerations of the process of either
taking up smoking or remaining a nonsmoker (5).
In another area of investigation, one project (19) is concerned not so
much with individual differences but with cultural differences in val-
ues, attitudes, and behavior related to smoking among various ethnic
groups in the Southwest and has as its main assumption the probable
existence of a common core of psychosocial factors operating to pro-
duce different motivation patterns among young people socialized in
a particular cultural environment.
Another kind of research-that of the controlled experiment
manipulating one variable at a time with a number of small sample


has advantages which were discussed at the 1967 behavior research
conference (8). The use of such laboratory methods and controls has
been shown to be particularly useful in communications research,
including the study of factors that affect a person's acceptance and use
of health information. More systematic efforts are needed which will
.relate the content of the message, the form of the message, the kind of
medium used, and the characteristics of the communicator to changes
in smoking behavior which are also related to the psychosocial nature
of the target audience. In particular, emotion-provoking communica-
tions need to be studied in relation to various factors that are known
to maintain actions, such as public commitment and conformity to
group norms
As in the case with epidemiological investigations, however, it is
probable that more prospective research studies combining social-
psychological, sociological, and anthropological concepts must be car-
ried out before a better understanding of smoking behavior initiation
(or non-initiation), continuation, or change can be achieved.

192


                     PEPTIC uLc!EB

(I) DOLL, R. Medical treatment of gastric ulcer. Scottish Medical Journal
   (Glasgow ) 9 (6) : 183-196, May 1984.
(8) DOLL, B, HILL, A. B. Mortality in relation to smoking: 10 years' observa-
   tions of British doctors. Part I, British Medical Journal (London)
    1 ( 5395) : 1399-1410, May 3S,l984.
(3) DOLL, R, HILL, A. B. Mortality in relation to smoking: 10 years' observa-
   tions of British doctors (concluded). British Medical Journal (London)
   l(S398) : 1450-1457, June 6,1964.
(4) FZBOUSOIV, E., Ja., REI~~TI~E, H., JB., HAEIUB, H. Analyais of 109 cases of
  perforated duodenal ulcers. The American Surgeon (Philadelphia)
   31(l) : 9-16, January 1986.
(5) EIAMMOND, E. C. Smoking in relation to mortality and morbidity. Findings
   in first 34 months of follow-up in a prospective study started in 1969.
   Journal of the National Cancer Institute (Washington) 33(5) : ll81-
   1188, May 1984.
(6) HAMXOXD, E. C. Smoking in relation to the death rates of 1 mllllon men
   and women. In: Haenssel, W., editor. Epidemiological Approaches to the
   Study of Cancer and Other Diseases. Bethesda, U.S. Public Health Serv-
   ice, National Cancer Institute Monograph No. 19, January l.968. pp. lZ7-
    !204.
(7) KAHXV, H. A. The Dorn study of smoking and mortality among U.S. vet-
   erans: report on 8% years of observation. In: Haenssel, W., editor.
   Epidemiological Approaches to the Study of Cancer and Other Diseases.
   Bethesda, U.S. Public Health Service, National Cancer Institute Mono-
   graph No. 19, January 1985. pp. l-125.
(8) KABA~E~, A., FOBE~&M, J. Social stress and living habits in the etiology
   of peptic ulcer. Annales Medlcinae Internal Fenniae (Helsinki) 85(l) :
   13-29,1988.
(9) KKQEKEB, E. J., Lnon, A. 5. The association of dlfhme obstructive pul-
   monary emphysema and chronic gastroduodenal ulceration. Diseases of
   the Chest (Chicago) 4!2(4) : 4l3-42l. October 1982.
(10) Lmr, P. Y. Studies on the pathogenesis of peptic ulcer. The Korean Jour-
   nal of Internal Medicine (Seoul) 6 (8) : 421-449, August 1983.
(11) MXTCEELL, R. S., FIL.LEY, G. F. Chronic obstructive bronchopulmonary dis-
   ease. I. Clinical features. American Review of Respiratory Diseases
   (Baltimore) 89(3) : 3t&371. March 1984.
(18) SMALL, W. R The recurrence of ulceration after surgery for duodenal
   ulcer. Journal of the Royal College of Surgeons of Edlnburg 9 : 266-978,
    July 1984.
(IS) U.S. PUBLIC HEALTH SEZB~~CE National Center for Health Statistics.
   Cigarette smoking and health characteristics, United StatepJuly 19l%
   June 1966. Washington, ULS. Department of Health, Education, and
   Welfare, Vital and Health Statlstlcs Series 10, No. 34, Public Health
    Service Publication No. l,ooO, May 1988.84 pp.
(14) U.S. PUBLIC HE~L~E SEBVICE. Smoking and Health. Report of the Advisory
   Committee to the Surgeon General of the Public Health Service. Wash-
   ington, U.S. Department of Health, Education, and Welfare, Pub&
   Health Service Publication No. 1,103, January 1984. 387 pp.


V1810Ir

(1) ADAMS, J. H., B~~crcwooo, W., WIUON, J. Further clInical and pathological
  observations on Leber's optic atrophy. Brain (London) 39: 163% March
    1966.

(3) BERGMAN, J. W., -WE, V., CHAO, P. The effect of cigarette smoking on
  the intraocular circulation. American Medical Association Archives of
  Ophthalmology (Chicago) 59 : W-488, April 1968.
(6) BOUIPIQ, G., COSCAS, G. Etude statistique et analytique de 164 Gas de
  nevrite optique alcoolo-tabaglque. Annales d'OculistiqUe (Paris) 199(19) :
   Q&974, ,1966.

(4) CARROLL, F. D. Nutritional amblyopia. Archives of Ophthalmology (CM-
  cage) 76 : 499-4l1, ~September 1966.
(5) c-4-v A, - Y. M., c?HEBNMB, A., LoWmm, Y., -NC, J.,
  Giuupmu, J. P. A propos de la r&cup&ation tardive de certaines neVriteS
  alcoolonicotiniques. Annales d'Oculistique (Paris) 199(10) : 995-994,
   November 1966.

(6) DBEYFUS, P. M. Blood transketolase levels in tobacco-alcohol amblyopia.
  Archives of Ophthalmology (Chicago) 74(6) : 617-6w), November 1965.
(7) DBEYFUS, P. M. Nutritional disorders of obscure etiology. Medical &ienCe
   (Philadelphia) : 4448, April 1966.

(8) Four.ns, W. 9. The ocular manifestations of blood diseases. Transactions of
  the Ophthalmological Society of the United Kingdom (London) 83:
   346-367, 1963.

(8) HALP~ZIN, M. H., MCFABLAND, R. A, Nrvmv, J. I., R~u~ET~N, F. J. W. The
   time course of the effects of carbon monoxide on visual thresholds. Journal
   of Physiology (London) 146: 533-693,1969.
(10) HEAT~N, J. M. Chronic cyanide poisoning and optic neuritis. Transactions
  of the Ophthalmological `Society of the United Kingdom (London) 39:
   263-!269,1962.

(11) JOHNSTON, D. M. A preliminary report of the effect of smoking on size of
   visual 5elds. Life Sciences (Oxford) 4(29) : !2215-9331, November 1963.
(12) JOHNSTON, D. M. Effect of smoking on visual search performance. Per-
   ceptual and Motor Skills (Missoula) 23: 6196!22,1966.
(IS) ~MOFABLAND, R. A. Tobacco and e5iciency. In: Human Factors in Air
  Transportation, Occupational Health and Safety. New York, McGraw-Hill,
   1953. Pp. 299-397.

(24) MCFAULAND, R. A., Moomr, R C. Human factors in highway safety. A review
  and evaluation. New England Journal of Medicine (Boston) 258 : -799.
   837-845,890-897, Apr. 25, May 2 and 9,1967.
(15) MCFAI~LAND, R. A., MOSELSX, A. L. Carbon monoxide in trucks and buses
   and information from other areas of research on carbon monoxide, alti-
   tude and cigarette smoking. In: Conference Proceedings: Health, medical
  and drug factors in highway safety. Washington, National Academy of
   Sciences-National Research Council Publication No. 338, 1964.
   Pp. 4.17-4.33.

(26) MCFARLAND, R. A., R~~oHTo~, F. J. W., H-xv, M. &, Nrvun, J. I. The
   effects of carbon monoxide and altitude on visual thresholds. Journal of
   Aviation Medicine (St. Paul) 16( 6) : 331-394, December 1944.
(17) MCLABEN, D. S. Nutritional disease and the eye. Borden's Review of Nutrl-
   tional Research (New York) 25: 1-16, January-March 1964.
(18) MIOLIOR, M., DE MO-A, V., SPINELLX, D. L'ambllopia alcoolicotabagica.
   Anna11 di Ottalmologia e Clinica Oculistica (Parma) 99: 649-669, No-
    vember 1964.

194


(19) RHI~, K, U., KIU, D. S., KIM, Y. 5. The effects of smoking on night ViSiOn.
   In: Tokyo, 14th Paciilc Medical Conference (Professional Papers) held
   Nov. 30-Dec. 2,1964,1966.6 pp.

(20) Rxvo0~11, A, GOLDBMITH, J. R., Hznwm, H. L, FINN, R, AND So~uwrrx, F.
   Estimating recent carbon monoxide exposures. A rapid method. Archives
   of Environmental Health (Chicago) 6(4) : 308-318, October, 1962.
(31) SABAUX, H., -BET, R., Br.~rs, B. Aspects actuels de la nevrite optlqpe de
   l'ethylique. Annales d'Oculistique (Paris) 199(10) : 943-954, November
    1966.

(28) ,SYITH, A D. M., DTJCKEIT, S. Cyanide, vitamin Bp, experimental demyeli-
   nation and tobacco amblyopia. British Journal of Experimental Pathology
   (London) 46 : 6X-622, December 1965.

(%I) Saarr.u, J. H. R Advances in ophthalmology. Praotltioner (London) 139 :
   467-471, October 1962.

(@4) U.S. PTJXLIC H~&L~H Sxzvxou Smoking and Health. Report of the Advisory
   Committee to the Surgeon ,General of the Public Health Service. Wash-
   ington, US. Department of Health, Education, and Welfare, 1964.387 pp.
(2.5) V~uroz, M. Tobacco-alcohol amblyopia. A critique of current concepts of this
   disorder, with special reference to the role of nutritional deficiency in its
   causation. Archives of Ophthalmology (Chicago) 70(3) : 313-318; Sep-
    tember 1963.

(26) VICTOB, M., Dzzrvne, P. M. Tobacco-alcohol amblyopia. Further comments
   on its pathology. Archives of Ophthalmology (Chicago) 74( 6) : 649887,
    November 1965.

(27) Wxnso~, J. Leber's hereditary optic atrophy: a possible defect of cyanide
   metabolism. Clinical Science (London) 29: 506516, December 1966.
(28) WILSON, J. Neurological blindness in children. Proceedings of the Royal
   `Society of Medicine (London) 60(Z) : 157-158, February 1967.

cIzBEoz1s

(1) DOLL, R., HILL, A. B. Mortality in relation to smoking: 10 years' observa-
   tions of British doctors. British Medical Journal (London) (Part I)
   1(5395) : l-1410, May 30,1964.

(2) DOLL, R., HILL, A. B. Mortality in relation to smoking: 10 years' observa-
  tions of British doctors (concluded). British Medical Journal (London)
   l(5396) : l-1467, June 6,1964.

(3) HAMMOND, E. C. Smoking in relation to the death rates of 1 million men
   and women, In: Haenszel, W., editor. Epidemiological Approaches to the
   Study of Cancer and other Chronic Diseases. Bethesda, US. Public Health
   Service, National Cancer Institute Monograph No. 19, January 1966.
   Pp. l27-204.

(4) K&IN, H. A. The Dorn ztudy of smoking and mortality among U.S. vet-
  erans: Report on 8% years of observation. In: Haenszel, W., editor.
   Epidemiological Approaches to the Study of Cancer and other Chronic
   Dlseases. Bethesda, US. Public Health Service, National Cancer Institute
   Monograph No. 19, January 1966. Pp. l-126.

(5) US. Public HEALTH Szsvroz Smoking and Health. Report of the Advisory
   Committee to the Surgeon General of the Public Health Service Wazh-
  ington, U.S. Department of Health, Education, and Welfare, PHS publica-
  tion No. 1,103, January 1964.337 pp.

196


                    PaEoKAKoT
(I) ~EBKATEY, J. Il., Giuacrvnmte, B. G., WELCB, H. B., m T. hf. Smoking
   aa an independent variable in a multiple regression analysis upon birth
   weight gestation. American Journal of Public Health and the Nation'8
   Health (New York) t%(4) : 625-83& April 1968.
(8) Assm, N. 5. Personal communication. June 6, 1907.
(8) B~mn, D. The epidemiology of prematurity. Journal of Pediatrica (St
   Louis) 65 (8, pt 1) : OOB-!X4, December 1064.
(4) BECKEB, B F., Kuvo, J. E. Studies on nicotine absorption during preg-
   nancy. II. The effects of acute heavy doses on mother and neonates.
   American Journal of Obstetrics and Gynecology (St. Louis) 95 : 5155%
    June 1056.
(5) BEBEBDEB, H., Waxes, W., Dmrr~oumuemt, J., SEAKHAEEIBI, Z., JACK~~OI~.
   E. The prediction of birth weight. A report from the Collaborative Study
   of Cerebral Palsy. March 1988. [Unpublished.] 243 pp.
(6) BUTLEB, N. B. The problems of low birth weight and early delivery. Jour-
   nal of Obstetrics and Gynecology of the British Commonwealth (Len-
   don) 72 : 1001-1002, December 1965.
(7) DAMON, A., Ntrrrac~, It. L, S-rut, E. J., S~~rzim, C. C., MCMAHOI$ B.
   Tobacco smoke as a possible genetic mutagen: parental amoklng and
   sex of children. American Journal of Epidemiology (Baltimore) 83(g) :
   53&53t$ lofkl.
(8) DAWKIHB, M. The biology of prematurity. Developmental Medicine and
   Child Neurology (London) 7 : 74-75, February 1965.
(9) Dowu~xo, C. C., C~apaaa~, W. E. Smoking and pregnancy. A statistical
   study of 5,659 patients. California Medicine (San Franclaco) 104 : 187,
    March 1968.
(10) EA~TMAX, N. J. Personal communication.
(II) Fa~vlarnr, J. F., Ja, Lv~DI~, F. B., Jn Smoking and pregnancy. Lancet
    (London) 1: 178,1964.
(18) Hrmori, H. J. The effects of smoking during pregnancy: a review with a
   preview. New Zealand Medical Journal (Wellington) 61: 545-548,
    November 1252.
(18) HOLYA~, (3. H., L~p~rrz, P. J. Effect of toxemic pregnancy on the fetus
   and neonate. Clinical Obstetrics and Gynecology (New York) O(4) :
    022434, December 1986.
(14) Ho-x, H. H., Ja A review of the literature concerning smoking during
   pregnancy. Virginia Medical Monthly (Bichmond) 92 : 274-270, June
    1985.
(15) JAXW~O~P, I. Aetiological factors in prematurity. Acta Obstetricia et Gyueco-
   logica Scandinavica (Stockholm) 45 : 27%200,1956.
(16) KIXO, J. E., Buoxmt, B. F. Studies on nicotine absorption during pregnancy,
   l.LD (50) for pregnant and nonpregnant rats. American Journal of
   Obstetrics and Gynecology (St. Louis) 95(4) : 508-514, June 15, 1966.
(17) KVMA& D., Zow, P. A. Studies on human premature births. II. In uiU0
   effect of smoking and in vitro effect of nicotine on human uterine con-
   tractility. American Journal of Obstetrics and Gynecology (St. Louis)
   87(3):413+W,Oct I.1953.
(28) MCDONALD, It. L., -FOB, C. F. EKects of smoking on selected clinical
   obstetric factors. Obstetrics and Gynecology (New York) 26(4) : 470-476,
    October 1965.
(29) MACMAHOX, B., ALPEBT, M., S~mxa, E. J. Infant weight and parental
   smoking habits. American Journal of Epidemiology (BaNimore) 82(g) :
    247~!26l, November 1985.

196


(20) MANTEL& C. D. Smoking in pregnancy : The role played by carbonic anhy-
   drase. New Zealand Medical Journal (Wellington) 63 : 6Ole Septem-
    ber 1964.

(ff ) Musnoo~, D. E. Birth weight and smoking. Nebraska State Medical Jour-
   nal (Lincoln) 4S(ll) : 604-606, November 1963.
(ff) NAOVIB, S. Y., Co~s~ocg, G. W., D~vm, H. J. Epidemiologic study of tri-
   chomoniasis in normal women. Obstetrics and Gynecology (New York)
   27 (5) : 607-316, May 1936.

(23) OVRSTED, M. Maternal constraint of foetal growth in man. Developmental
   Medicine and Child Neurology (London) 7 : 479-401, October 1965.
(ad) Psrxsso~, W. F., Mosses, K. N., KALTBEIDEB, D. F. Smoking and prematur-
   ity. A preliminary report based on study of 7,740 Caucasians. Obstetrics
   and Gynecology (St Louis) 26( 6) : 775-779, December 1965.
(25) RAVENHOLT, R. T., Lsvrns~1, 116. J. Smoking duriug pregnancy. Laxmet
   (London) 1: 961, May 1865.

(es) R~~E~~EoLT, R. T., L~~IIPBKI, M. J., NELLIST, D. J., TAKEXAGA, M. Effects
   of smoking upon reproduction. American Journal of Obstetrics and
   Gynecology (St. Louis) 06(Z) : 26-281, Sept. 15. 1966.
(87) R~IRKE, W. A, Hus~imsou, Bf. Smoking and prematurity in the presence
   of other variables. Archives of Environmental Health (Chicago) 12 :
   6OO-603, May 1086.

(38) RVSSEZL, C. S., TAYLOB R. Some effects of smoking in pregnancy. Journal
  of Obstetrics and Gyuecology of the British Commonwealth (London)
   73 : 742-74& october 1966.

(29) STEELS, R., LA~QWOBTH, J. T. The relationahlp of antenatal and postnatal
   factors to sudden unexpected death in infancy. Canadian Medical Asso-
   ciation Journal (Toronto) 94: 1165-1171, May 28, 1966.
(SO) U~PDEBWOOD, P., HESTEB, L. L., Laprrra, T., Ja, Gauss, K. V. The relationship
   of smoking to the outcome of pregnancy. American Journal of Obstetrics
   and Gynecology (St. Louis) Ol(2) : 27CX!76, Jan. 15, 1965.
(31) Ux~xswoon, P. B., Kssrxs, K. F., O'Lans, J. M., CALLA~AX, D. A. Parental
   smoking empirically related to pregnancy outcome. June 1988. [Unpub-
   lished.] 22 pp.

(32) UIDEBWOOD, P. B., Kss~im, K. F., O'LAHB, J. M., C~AXV, D. A. Parental
   smoking empirically related to pregnancy outcome. Obstetrics and Gyne-
   cology (New York) 29( 1) : l-8. January 1967.
(33) U.S. PUBLIC HEALTH SEBV~C~ Smoking and Health. Report of the Advisory
   Committee to the Surgeon General of the Public Health Service. [Wash-
   ington] U.S. Department of Health, Education, and Welfare, 1884.387 pp.
(34) YEBVSHALMY, J. Mother's cigarette smoking and survival of infant.
   American Journal of Obstetrics and Gynecology (St. Louis) 88(4) :
   585-518, Feb. 15,1384.

(35) YOUNO, I. M., PVOE, L. G. C. E. The carbon monoxide content of foetal
   and maternal blood. Journal of Obstetrics and Gynecology of the Brit-
   ish Commonwealth (London) 70(4) : 681+%4, 196%
(36) ZABBISKIE, J. R. Effect of cigarette smoking during pregnancy. Study of
   2,888 cases. Obstetrics and Gynecology (New York) 21(4) : 405-411, April
    1383.
                      ACCIDENTS

(I) hAMa, J. R. Oral habits and traillc accidents : Overdependency as explana-
  tory construct. Presented at the Fourth International Conference on
  Alcohol and Tragic Safety, Bloomington, Ind., Dec. 5-9, 1885. [Unpub-
   lished.]

197


(3) ALLEN, B. V. An investigation of the relationship between smoking aud
  personality. Submitted to the Committee on Graduate Study of the Uni-
  versity of Portland in partial fulflllment of the requirements for the de-
  gree of Master of Science. Portland, University of Portland, 1058.
   [Unpublishti]

(8 ) ISXM~T, A. P. Personal communication.
(4) MCFABLA~D, R. A., Mooan, R C. Human factors in highway safety (con-
  cluded). A review and evaluation. New England Journal of Medicine
   (Boston) 255(19) : 800-807, May 9.1957.
(5) National Fire Protection Association, Boston. Personal communication.
(6) NAVS, A., EROLEB, V., HETYCEOVA, M., VAVBBCKOVA, 0. Work' injuries and
  smoking. Industrial Medicine and Surgery (Sheboygan) 35 (10) : 880-
   331, October 1033.

(7) U.S. PWLIC HEALTH Sssvrcs. Estimates of deaths from fire and explosion
  and hot substances. Epidemiology and Surveillance Injury Control Pro-
  gram, U.S. Public Health Service, Nov. 14, 1056. [Unpublished.] 3 pp.

Ps~cuosocx~~ Asrsor b Sarounvo

(1) COAM, R. W. Research strategy in the investigation of personality cor-
  relates. Ia: National Research Conference on Smoking Behavior, Re
  search Reports, vol. I, pp. gl-14, University of Arisona, Tucson, Aris.,
   Mar. 3431, Apr. l,lOt33.

(I) F~srrnoxa L. A theory of cognitive dissonance. Second edition, Stanford
  University Press, Stanford, Calif., 1062.201 pp.
(3) HOCHBAVM, G. M. Public participation in medical screening programs,
  Government Printing Otllce, U.S. Pnblic Health Service Publication No.
   572, Washington, 1053.

(4) HOCHBAVY, G. M. Behavior in response to health threats. Paper presented
  at the Annual `Meeting of the American Psychological Association, Chi-
   cago, Ill., 1060.

(5) HOB~P, D. An analytic approach to the smoking problem. Paper presented
  at the meeting of the National Congress of Parents and Teachers, Chi-
   cago, Ill., Sept. 23,1086.

(6) HOEN, D. Wauvoaow, 9. Some dimensions of a model for smoking behavior
  change. In: From Epidemiology to Ecology-A Panel Discussion, Smoking
   and Health in Transition. American Journal of Public Health and the
  Nation's Health (New York) 543 (12, -Pt. 2) : 21-26, Supplement to Decem-
   ber 1036.

(7) HOBX~P, K. The neurotic personality of our time. W. W. Norton, New York,
   1937.

(8) L~~~THAL, H. Intervention: Nonclinical experimental research. Paper
  presented at the 1987 National Research Conference on Smoking and
  Public Health, University of Wisconsin, Madison, May l-3, 1987.
(9) MAVSMEB, B., PLAY, E. S. Behavioral aspects of smoking: A conference
  report. Health Education Monographs (New York), Supplement No. 2,
   1966 58 pp.

(16) MCK~X~ELL, A. C. A comparison : British experiences in smoking research.
   Paper presented at the 1967 National Research Conference on Smoking
   and Public Health, University of Wisconsin, Madison, May 1-3, 1067.
(II) 1987 National Research Conference on Smoking and Public Health, (BOB-
   ~AITA, E. F., EVAHS, R R., editors, to be published), held at the Univer-
   sity of Wisconsin, Madison, May l-3,1037.

198


(18) Roannaroc~, I. M., HOCEBAVM, G. M., Ksanrxa, S. 5. Determinants of
   health behavior. White House Conference on Children and Youth, Wash-
   ington, 1330.

(a) SCHWABT~, J. L., DVBIT~~Y, M. Clinical intervention. Paper presented at
   the 1387 National Research Conference on Smoking and Public Health,
   University of Wisconsin, Madison, May l-3,1387.
(14) STBAITS, B. C. Sociological and psychological correlates of adoption and
   discontinuation of cigarette smoking. Report to the Council for Tobacco
   Research, U.S.A. The University of Chicago, Chicago, Ill, July 1986.
(15) To~xrns, 53. 5. Psychological model for smoking behavior. In: From Epi-
   demiology to Ecology-A Panel Discussion, Smoking and Health in Tran-
   sition. Amerian Journal of Public Health and the Nation's Health (New
   York) 56 (12, Pt. 2) : 17-20, Supplement to December 1338.
(16) To~iuns, S. S. Theoretical fmplications and guidelines to future research.
   In: Behavior Aspects of Smoking: A Conference Report. Health Educa-
   tion Monographs (New York) Supplement No. 2, 1333. pp. 35-48.
(17) U.S. Pus~zc HEALTH SEBVICE. Psychosocial aspects of smoking, chapter 14.
   In: Smoking and Health. Report of the Advisory Committee to the Sur-
   geon General of the Public Health Service, U.S. Department of Health,
   Education, and Welfare (Washington), Public Health Service Publiea-
   tion No. 1103, January 1384. pp. 339379.

(18) WAI~~~KIW, 5.. HOBN, D., Iu~an, F. Dosage patterns of cigarette smoking
   in American adults. Paper presented at the annual meeting of the Amer-
   ican Public Health Association, Epidemiology and School Health Sec-
   tions Session, San Francisco, Calif., Nov. 2,1333.

(19) 5130~4 5. V., JONMI, R. D., CHIUSTL~NO, C. J., JOHNBOX, J. A., Ldwamvc~,
   R.,HONOYICHL, R. E., ANTINOBO, N. W. A cross-cultural study of smoking
   behavior and related variables for students attending eight high schoola
   in Arizona. In: National Research Conference on Smoking Behavior, Re-
   search Reports, vol. III, University of Arizona, Tucson, Aria., Mar. 30,31,
   Apr. 1,1936.

(PQ) ZAQONA, S. V. Identifying psychological correlates of smoking behavior.
   Paper presented at the 1337 National Research Conference on Smoking
   and Public Health, University of Wisconsin, Madison, May l-3, 1967.
(21) ~QONA, S. V. editor. Studies and issues in smoking behavior. (In press,
   University of Arizona Press, July, 1387). Papers and discussions at the
   National Research Conference on Smoking Behavior, University of Ari-
   zona, Tucson, Ariz., Mar. 30,31, Apr. 1, 1966.

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