RFE Fish Species Identification Isoelectric Focusing Tools - Yellowtail Flounder Phast IEF Gel 01

U.S. Food & Drug Administration
Seafood Products Research Center
Center for Food Safety & Applied Nutrition
Regulatory Fish Encyclopedia:
IEF Gel Lane Banding Pattern Peak Report Data

Peak Report Data from Isoelectric Focusing for a specimen of:
Yellowtail Flounder ---- Go to Display Gel Lane Navigator.

FDA Seafood List Scientific Name: Pleuronectes ferrugineus
FDA Seafood List Common Name: Yellowtail Flounder
FDA Seafood List Market Name: Flounder
Unique IEF Gel Code: 19980331gel04
Unique Web Code: yfh01Srr
Tissue Aliquot Code: pleuferr H94-003 #02a Gel Analysis Conditions


Gel Image	Fish Image	Calibrated Gel

DISPLAY GEL LANE NAVIGATOR
RETURN TO TOP OF THIS DOCUMENT
Lane a pI Markers	Lane b Specimen	Lane c pI Markers	Lane d Co-Migrated	Lane e Specimen	Lane f pI Markers	Go to Gel Schematic

**DISPLAY GEL LANE LETTER:** Lane a
**DIGITAL REPORT GEL LANE:** 2
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 2
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.55	35	4.5	0.0986736	4	0.684312	7.7	5.8	4.55	35.2	4.1	0.095185
2	5.2	130	16.6	0.103613	4	0.711088	8	6	5.2	130.5	4.1	0.0978832
3	5.85	243	31	0.0697408	5	0.570823	6.4	4.8	5.85	243.2	4.9	0.0654466
4	6.55	387	49.4	0.179345	6	1.66195	18.6	14	6.55	386.6	5.7	0.163214
5	6.85	423	54	0.0299424	5	0.24989	2.8	2.1	6.85	422.6	5.2	0.0273053
6	7.35	492	62.8	0.0903005	4	0.578574	6.5	4.9	7.35	492.2	3.7	0.0886782
7	8.15	617	78.7	0.0501731	12	1.13849	12.8	9.6	8.15	615.1	12	0.0535564
8	8.45	674	86	0.147113	9	2.23822	25.1	18.9	8.45	673.5	8.7	0.144651
9	8.65	724	92.3	0.122062	5	0.944182	10.6	8	8.65	723.8	4.6	0.116187
10	9.3	771	98.3	0.0219353	3	0.143505	1.6	1.2	9.3	770.7	3.5	0.0232831

PeakCounter: 10

**DISPLAY GEL LANE LETTER:** Lane b
**DIGITAL REPORT GEL LANE:** 3
**LANE TYPE:** Specimen
**ACTUAL GEL LANE:** 3
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.575	29	3.7	0.0360733	8	0.526214	2.3	1.9	4.575	28.3	8.2	0.0362512
2	4.845	67	8.6	0.236967	14	6.07702	26.6	22.2	4.845	67.3	14.1	0.243506
3	5.044	95	12.1	0.0525456	12	1.15095	5	4.2	5.044	89.5	12.4	0.0524256
4	5.469	166	21.2	0.301832	4	2.20963	9.7	8.1	5.469	165.9	4.5	0.278475
5	5.595	189	24.2	0.252264	4	1.8251	8	6.7	5.595	188.8	4.1	0.250229
6	5.661	201	25.7	0.0830354	5	0.741633	3.3	2.7	5.661	201.5	5.4	0.0771228
7	5.727	213	27.2	0.0323014	3	0.173565	0.8	0.6	5.727	212.7	3.4	0.0287036
8	5.77	221	28.3	0.0111949	1	0.0192769	0.1	0.1	5.77	221.6	1.2	0.00919068
9	5.842	234	29.9	0.375038	5	3.1914	14	11.6	5.842	232.9	4.5	0.39919
10	6.033	273	34.9	0.0433639	2	0.172638	0.8	0.6	6.033	272.9	2.3	0.042209
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	6.13	293	37.5	0.060546	9	0.876036	3.8	3.2	6.13	295	9.1	0.0544584
12	6.262	320	40.9	0.14265	13	3.10301	13.6	11.3	6.262	321.6	13.1	0.133839
13	6.389	346	44.2	0.230911	2	0.690418	3	2.5	6.389	346.2	1.9	0.209458
14	6.719	399	51	0.254119	5	2.05628	9	7.5	6.719	399.2	4.6	0.254166

PeakCounter: 14

**DISPLAY GEL LANE LETTER:** Lane c
**DIGITAL REPORT GEL LANE:** 4
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 4
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.55	18	2.3	0.0714567	4	0.460999	4.8	3.6	4.55	18.9	3.8	0.068269
2	5.2	106	13.6	0.0684247	5	0.541013	5.6	4.2	5.2	107	4.8	0.0630643
3	5.85	230	29.5	0.0775457	5	0.626442	6.5	4.8	5.85	229.7	5	0.0701627
4	6.55	373	47.8	0.139897	8	1.76885	18.3	13.6	6.55	372.7	7.7	0.128931
5	6.85	408	52.3	0.0224947	7	0.263838	2.7	2	6.85	409.1	7.3	0.0202762
6	7.35	481	61.7	0.0587811	4	0.373267	3.9	2.9	7.35	480.7	3.8	0.0561466
7	8.15	599	76.8	0.0807163	14	1.78254	18.5	13.8	8.15	600.5	14.4	0.0697413
8	8.45	660	84.6	0.147256	9	2.17304	22.5	16.8	8.45	658.7	8.6	0.142383
9	8.65	712	91.3	0.121289	5	0.961207	10	7.4	8.65	711.4	4.7	0.115475
10	9.3	763	97.8	0.0794659	5	0.704589	7.3	5.4	9.3	762.8	5.3	0.0751959

PeakCounter: 10

**DISPLAY GEL LANE LETTER:** Lane d
**DIGITAL REPORT GEL LANE:** 5
**LANE TYPE:** Co-Migrated
**ACTUAL GEL LANE:** 5
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.613	29	3.7	0.0994945	5	0.773529	2.9	2.5	4.613	29.2	4.7	0.0931224
2	4.863	69	8.9	0.261087	15	6.83406	25.8	22	4.863	71.1	14.6	0.26399
3	5.2	123	15.9	0.0583365	5	0.460572	1.7	1.5	5.2	123	4.6	0.0562382
4	5.44	162	20.9	0.258282	6	2.47193	9.3	8	5.44	163	5.5	0.25278
5	5.618	191	24.6	0.248684	5	2.25631	8.5	7.3	5.618	191.9	5.1	0.250553
6	5.704	205	26.4	0.0905517	6	0.924681	3.5	3	5.704	205	6.3	0.0828014
7	5.784	218	28.1	0.0215802	12	0.302316	1.1	1	5.784	214	12.3	0.0138387
8	5.897	238	30.7	0.366491	5	3.43025	13	11	5.897	236.8	5.1	0.378999
9	6.083	275	35.4	0.0286262	3	0.156964	0.6	0.5	6.083	275.4	3.1	0.0288451
10	6.198	298	38.4	0.0592038	11	1.0498	4	3.4	6.198	299.1	10.6	0.0561422
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	6.314	321	41.4	0.141046	12	2.74406	10.4	8.8	6.314	322.8	11.6	0.133979
12	6.444	347	44.7	0.170853	4	1.07946	4.1	3.5	6.444	346.9	3.6	0.170987
13	6.55	368	47.4	0.0291749	4	0.221555	0.8	0.7	6.55	367.6	4.1	0.0308471
14	6.799	399	51.4	0.202759	5	1.76033	6.7	5.7	6.799	398.8	5.1	0.196593
15	7.35	478	61.6	0.030523	5	0.24122	0.9	0.8	7.35	478.6	4.6	0.0293533
16	8.15	606	78.1	0.0310637	9	0.4559	1.7	1.5	8.15	604.6	8.7	0.0295462
17	8.45	665	85.7	0.0596116	8	0.782904	3	2.5	8.45	663.8	7.8	0.0568344
18	8.65	713	91.9	0.0523865	4	0.384573	1.5	1.2	8.65	712.4	4.4	0.0489793
19	9.3	759	97.8	0.0226737	3	0.133154	0.5	0.4	9.3	758.8	3.3	0.0227912

PeakCounter: 19

**DISPLAY GEL LANE LETTER:** Lane e
**DIGITAL REPORT GEL LANE:** 6
**LANE TYPE:** Specimen
**ACTUAL GEL LANE:** 6
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.505	13	1.7	0.0557946	8	0.746767	3.1	2.6	4.505	14.3	7.6	0.0557909
2	4.75	51	6.6	0.323031	13	7.35536	30.6	25.2	4.75	56	13.1	0.315634
3	4.97	85	10.9	0.0425227	10	0.479017	2	1.6	4.97	82.8	10	0.0270735
4	5.343	144	18.5	0.367328	5	2.47564	10.3	8.5	5.343	145.7	4.5	0.310303
5	5.501	170	21.9	0.333748	4	2.1987	9.2	7.5	5.501	170.9	3.8	0.327218
6	5.599	186	23.9	0.111354	7	1.23228	5.1	4.2	5.599	185.6	7	0.0987877
7	5.684	200	25.7	0.0282817	3	0.161067	0.7	0.6	5.684	201.1	3.3	0.027852
8	5.751	211	27.2	0.0201411	2	0.0454179	0.2	0.2	5.751	210.9	1.6	0.0162474
9	5.811	221	28.4	0.355965	2	1.3928	5.8	4.8	5.811	220.6	2.2	0.353559
10	5.842	226	29.1	0.365455	3	1.96903	8.2	6.7	5.842	226.3	3.1	0.362011
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	6.054	268	34.5	0.0295098	2	0.130069	0.5	0.4	6.054	267.9	2.5	0.0297288
12	6.154	288	37.1	0.050146	10	0.832818	3.5	2.9	6.154	289.5	9.8	0.047743
13	6.294	316	40.7	0.106839	13	2.22812	9.3	7.6	6.294	316.7	12.7	0.0991834
14	6.435	344	44.3	0.185604	2	0.742097	3.1	2.5	6.435	343.7	2.3	0.183763
15	6.769	393	50.6	0.253516	5	2.00916	8.4	6.9	6.769	392.7	4.5	0.251063

PeakCounter: 15

**DISPLAY GEL LANE LETTER:** Lane f
**DIGITAL REPORT GEL LANE:** 7
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 7
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.55	22	2.8	0.104071	4	0.781566	8.5	6.3	4.55	21.9	4.5	0.0989292
2	5.2	119	15.4	0.134313	4	0.804233	8.7	6.5	5.2	118.7	3.6	0.125557
3	5.85	227	29.3	0.0890073	5	0.68365	7.4	5.5	5.85	226.8	4.7	0.0819249
4	6.55	367	47.4	0.165183	6	1.56748	17	12.6	6.55	366.5	5.9	0.15107
5	6.85	401	51.7	0.0312133	5	0.248102	2.7	2	6.85	400.5	5	0.0280655
6	7.35	473	61	0.0627002	4	0.408775	4.4	3.3	7.35	472.6	3.8	0.0599389
7	8.15	599	77.3	0.0775528	11	1.47373	16	11.9	8.15	596.7	10.8	0.0771902
8	8.45	654	84.4	0.13959	7	1.73996	18.8	14	8.45	653.6	7.3	0.134984
9	8.65	704	90.8	0.108455	5	0.827513	9	6.7	8.65	703.4	4.5	0.102772
10	9.3	755	97.4	0.0676537	6	0.702238	7.6	5.6	9.3	754.2	6.5	0.0614141

PeakCounter: 10

Gel Banding Patterns Display in HTML using GIF stacking technique
(Uses Migration Position Normalized Data from above text peak reports)
Return to Lane Navigator Go to Gel Schematic Key

a b c d e f

MK SP MK CO SP MK

pI 4.55 label for gel schematic

pI 5.20 label for gel schematic

pI 5.8 label for gel schematic

pI 6.55 label for gel schematic

pI 6.85 label for gel schematic

pI 7.35 label for gel schematic

pI 8.15 label for gel schematic

pI 8.45 label for gel schematic

pI 8.65 label for gel schematic

pI 9.30 label for gel schematic

pI 4.55 label for gel schematic

pI 5.20 label for gel schematic

pI 5.8 label for gel schematic

pI 6.55 label for gel schematic

pI 6.85 label for gel schematic

pI 7.35 label for gel schematic

pI 8.15 label for gel schematic

pI 8.45 label for gel schematic

pI 8.65 label for gel schematic

pI 9.30 label for gel schematic

Key to Gel Schematic:

MK=pI Marker Lanes, pI bands are in red
SP=Specimen Lanes, Specimen bands are in blue
CO=Co-migrated Lanes of pI Markers and Specimen (pI bands are in red, Specimen bands are in blue)
Note: In co-migrated lanes, some pI Markers and Specimen bands may overlap in experiment. Red pI
band is reported when overlapped peak position is equal to nominal pI Marker position. Blue Specimen
band is reported when overlapped peak position deviates slightly from nominal pI Marker position.
Weak (<2% rel. intensity), Medium (2-6% rel. intensity), and Strong (>6% rel. intensity) band intensities indicated by gel schematic band thickness.
Return to Top of Gel Schematic

PeakCount: 78

HTML Forms TextArea Normalized Peak-Position Peak-Area for Copy-Paste events
4.550 7.700 5.200 8.000 5.850 6.400 6.550 18.600 6.850 2.800 7.350 6.500 8.150 12.800 8.450 25.100 8.650 10.600 9.300 1.600 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane a DIGITAL REPORT GEL LANE: 2 LANE TYPE: pI Markers ACTUAL GEL LANE: 2 PeakCount: 10 Return to Lane Navigator
4.575 2.300 4.845 26.600 5.044 5.000 5.469 9.700 5.595 8.000 5.661 3.300 5.727 0.800 5.770 0.100 5.842 14.000 6.033 0.800 6.130 3.800 6.262 13.600 6.389 3.000 6.719 9.000 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane b DIGITAL REPORT GEL LANE: 3 LANE TYPE: Specimen ACTUAL GEL LANE: 3 PeakCount: 14 Return to Lane Navigator
4.550 4.800 5.200 5.600 5.850 6.500 6.550 18.300 6.850 2.700 7.350 3.900 8.150 18.500 8.450 22.500 8.650 10.000 9.300 7.300 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane c DIGITAL REPORT GEL LANE: 4 LANE TYPE: pI Markers ACTUAL GEL LANE: 4 PeakCount: 10 Return to Lane Navigator
4.613 2.900 4.863 25.800 5.200 1.700 5.440 9.300 5.618 8.500 5.704 3.500 5.784 1.100 5.897 13.000 6.083 0.600 6.198 4.000 6.314 10.400 6.444 4.100 6.550 0.800 6.799 6.700 7.350 0.900 8.150 1.700 8.450 3.000 8.650 1.500 9.300 0.500 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane d DIGITAL REPORT GEL LANE: 5 LANE TYPE: Co-Migrated ACTUAL GEL LANE: 5 PeakCount: 19 Return to Lane Navigator
4.505 3.100 4.750 30.600 4.970 2.000 5.343 10.300 5.501 9.200 5.599 5.100 5.684 0.700 5.751 0.200 5.811 5.800 5.842 8.200 6.054 0.500 6.154 3.500 6.294 9.300 6.435 3.100 6.769 8.400 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane e DIGITAL REPORT GEL LANE: 6 LANE TYPE: Specimen ACTUAL GEL LANE: 6 PeakCount: 15 Return to Lane Navigator
4.550 8.500 5.200 8.700 5.850 7.400 6.550 17.000 6.850 2.700 7.350 4.400 8.150 16.000 8.450 18.800 8.650 9.000 9.300 7.600 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane f DIGITAL REPORT GEL LANE: 7 LANE TYPE: pI Markers ACTUAL GEL LANE: 7 PeakCount: 10 Return to Lane Navigator

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EXPERIMENTAL CONDITIONS: Gels were scanned at 600 d.p.i. in Adobe Photoshop 3.02 using a Sharp JX-325 Scanner with Film Scanner Attachment. Gels were analyzed for Densitometry, Intregration, and Migration Position Normalization in EXCEL 5.0 Format. *.xls files were imported into Paradox 7.0 to save as *.db files.

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Delphi 4.0 Generation of this Isoelectric Focusing (IEF) RFE PAGE 5 Tools in HTML
This HTML is automatically generated from a series of Paradox 7.0 database filesof IEF peak reports is controlled with a main RFEIEF Paradox 7.0 database withDelphi 3.0 front end (see Delphi 2.0 form) and back end for facile addition and modification of the Content of these RFE PAGE 5 HTML tools.
Contact Brad Tenge at btenge@ora.fda.gov for details.

RFE Contacts
A summary list of RFE contact personnel is available at http://www.cfsan.fda.gov/~frf/rfecon.html.

For isoelectric focusing laboratory experiments for fish species identification, you may contact

Brad Tenge at E-mail: btenge@ora.fda.gov
Ngoc-Lan Nguyen at E-mail: nnguyen@ora.fda.gov
Jim Barnett at E-mail: jbarnett@ora.fda.gov
Pat Rogers at E-mail: pxr@cfsan.fda.gov

For isoelectric focusing gel analysis and pattern recognition applications, you may contact

Brad Tenge at E-mail: btenge@ora.fda.gov
Ngoc-Lan Nguyen at E-mail: nnguyen@ora.fda.gov
Fred Fry at E-mail frf@vm.cfsan.fda.gov

For CFSAN web consultation and posting, you may contact

Fred Fry E-mail: frf@vm.cfsan.fda.gov

Thank you for using the Regulatory Fish Encyclopedia (RFE)!

RFE Species Browse | RFE Tutorial | Seafoods | CFSAN

Last updated on 2003-FEB-27 by bjt/frf/nln

Peak Report Data from Isoelectric Focusing for a specimen of: Yellowtail Flounder ---- Go to Display Gel Lane Navigator.

Peak Report Data from Isoelectric Focusing for a specimen of:
Yellowtail Flounder ---- Go to Display Gel Lane Navigator.