Bibliographic Citation
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Title | Influence of manganese on growth of a sheathless strain of Leptothrix discophora |
Creator/Author | Adams, L.F. ; Ghiorse, W.C. |
Publication Date | 1985 Mar 01 |
OSTI Identifier | OSTI ID: 5748339 |
Other Number(s) | CODEN: AEMID |
Resource Type | Journal Article |
Resource Relation | Appl. Environ. Microbiol. ; Vol/Issue: 49:3 |
Research Org | Cornell Univ., Ithaca, NY |
Subject | 560302 -- Chemicals Metabolism & Toxicology-- Microorganisms-- (-1987); BACTERIA-- GROWTH;MANGANESE-- TOXICITY; BATCH CULTURE;CATIONS;IRON;NUTRIENTS;RESPONSE MODIFYING FACTORS;SURVIVAL TIME |
Related Subject | CHARGED PARTICLES;ELEMENTS;IONS;METALS;MICROORGANISMS;TRANSITION ELEMENTS |
Description/Abstract | Mn/sup 2 +/ exerted various effects on the growth of Leptothrix discophora strain SS-1 in batch cultures depending on the concentration added to the medium.^Concentrations of 0.55 to 5.5 ..mu..M Mn/sup 2 +/, comparable to those in the environment from which strain SS-1 was isolated, decreased cell yield and prolonged stationary-phase survival, but did not affect growth rate.^Elevated concentrations of 55 to 910 3=M Mn/sup 2 +/ also decreased cell yield and prolonged survival, but growth rate was decreased as well.^The addition of 1820 ..mu..M Mn/sup 2 +/ caused a decline in cell numbers followed by an exponential rise after 80 h of incubation, indicating the development of a population of cells resistant to Mn/sup 2 +/ toxicity.^When 360 ..mu..M Mn/sup 2 +/ or less was added to growth flasks, Mn/sup 2 +/ was oxidized to manganese oxide (MnO/sub x/, where x is approx.2), which appeared as brown particles in the medium.^Quantification of Mn oxidation during growth of cultures to which 55 ..mu..M Mn/sup 2 +/ was added showed that nearly all of the Mn/sup 2 +/ was oxidized by the beginning of the stationary phase of growth (15 to 25 h).^This result suggested that the decrease in cell yield observed at low and moderate concentrations of Mn/sup 2 +/ was related to the formation of MnO/sub x/, which may have bound cationic nutrients essential to the growth of SS-1.^The addition of excess Fe/sup 3 +/ to cultures containing 55 ..mu..M Mn/sup 2 +/ increased cell yield to levels near those found in cultures with no added Mn/sup 2 +/, indicating that iron deprivation by MnO/sub x/ was at least partly responsible for the decreased cell yield. |
Country of Publication | United States |
Language | English |
Format | Pages: 556-562 |
System Entry Date | 2001 May 13 |
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