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Title Influence of manganese on growth of a sheathless strain of Leptothrix discophora
Creator/Author Adams, L.F. ; Ghiorse, W.C.
Publication Date1985 Mar 01
OSTI IdentifierOSTI ID: 5748339
Other Number(s)CODEN: AEMID
Resource TypeJournal Article
Resource RelationAppl. Environ. Microbiol. ; Vol/Issue: 49:3
Research OrgCornell Univ., Ithaca, NY
Subject560302 -- Chemicals Metabolism & Toxicology-- Microorganisms-- (-1987); BACTERIA-- GROWTH;MANGANESE-- TOXICITY; BATCH CULTURE;CATIONS;IRON;NUTRIENTS;RESPONSE MODIFYING FACTORS;SURVIVAL TIME
Related SubjectCHARGED PARTICLES;ELEMENTS;IONS;METALS;MICROORGANISMS;TRANSITION ELEMENTS
Description/Abstract Mn/sup 2 +/ exerted various effects on the growth of Leptothrix discophora strain SS-1 in batch cultures depending on the concentration added to the medium.^Concentrations of 0.55 to 5.5 ..mu..M Mn/sup 2 +/, comparable to those in the environment from which strain SS-1 was isolated, decreased cell yield and prolonged stationary-phase survival, but did not affect growth rate.^Elevated concentrations of 55 to 910 3=M Mn/sup 2 +/ also decreased cell yield and prolonged survival, but growth rate was decreased as well.^The addition of 1820 ..mu..M Mn/sup 2 +/ caused a decline in cell numbers followed by an exponential rise after 80 h of incubation, indicating the development of a population of cells resistant to Mn/sup 2 +/ toxicity.^When 360 ..mu..M Mn/sup 2 +/ or less was added to growth flasks, Mn/sup 2 +/ was oxidized to manganese oxide (MnO/sub x/, where x is approx.2), which appeared as brown particles in the medium.^Quantification of Mn oxidation during growth of cultures to which 55 ..mu..M Mn/sup 2 +/ was added showed that nearly all of the Mn/sup 2 +/ was oxidized by the beginning of the stationary phase of growth (15 to 25 h).^This result suggested that the decrease in cell yield observed at low and moderate concentrations of Mn/sup 2 +/ was related to the formation of MnO/sub x/, which may have bound cationic nutrients essential to the growth of SS-1.^The addition of excess Fe/sup 3 +/ to cultures containing 55 ..mu..M Mn/sup 2 +/ increased cell yield to levels near those found in cultures with no added Mn/sup 2 +/, indicating that iron deprivation by MnO/sub x/ was at least partly responsible for the decreased cell yield.
Country of PublicationUnited States
LanguageEnglish
FormatPages: 556-562
System Entry Date2001 May 13

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