A large array of human monoclonal antibodies to type 1 human immunodeficiency virus from combinatorial libraries of asymptomatic seropositive individuals.

Proc Natl Acad Sci U S A. 1991 November 15; 88(22): 10134–10137.

PMCID: PMC52882

D R Burton, C F Barbas, 3rd, M A Persson, S Koenig, R M Chanock, and R A Lerner

Department of Molecular Biology, Scripps Research Institute, La Jolla, CA 92037.

This article has been cited by other articles in PMC.

Abstract

A panel of human monoclonal antibody Fab fragments has been generated against the surface glycoprotein gp120 of type 1 human immunodeficiency virus (HIV) by antigen selection from a random combinatorial library expressed on the surface of filamentous phage. The library was prepared from 5 ml of bone marrow from an asymptomatic individual who has been HIV-positive for 6 years. The antibodies have high affinity for antigen (mostly with affinity constants of greater than 10(8) M-1) and notable sequence diversity. Given appropriate donor selection, the methods described should allow the generation of antibodies for the evaluation of passive immunization as a therapy for AIDS.

Full text

Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (983K), or click on a page image below to browse page by page. Links to PubMed are also available for Selected References.

Images in this article

Image
on p.10137

Click on the image to see a larger version.

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

Jackson, GG; Perkins, JT; Rubenis, M; Paul, DA; Knigge, M; Despotes, JC; Spencer, P. Passive immunoneutralization of human immunodeficiency virus in patients with advanced AIDS. Lancet. 1988 Sep 17;2(8612):647–652. [PubMed]
Karpas, A; Hill, F; Youle, M; Cullen, V; Gray, J; Byron, N; Hayhoe, F; Tenant-Flowers, M; Howard, L; Gilgen, D, et al. Effects of passive immunization in patients with the acquired immunodeficiency syndrome-related complex and acquired immunodeficiency syndrome. Proc Natl Acad Sci U S A. 1988 Dec;85(23):9234–9237. [PubMed]
Rossi, P; Moschese, V; Broliden, PA; Fundaró, C; Quinti, I; Plebani, A; Giaquinto, C; Tovo, PA; Ljunggren, K; Rosen, J, et al. Presence of maternal antibodies to human immunodeficiency virus 1 envelope glycoprotein gp120 epitopes correlates with the uninfected status of children born to seropositive mothers. Proc Natl Acad Sci U S A. 1989 Oct;86(20):8055–8058. [PubMed]
Devash, Y; Calvelli, TA; Wood, DG; Reagan, KJ; Rubinstein, A. Vertical transmission of human immunodeficiency virus is correlated with the absence of high-affinity/avidity maternal antibodies to the gp120 principal neutralizing domain. Proc Natl Acad Sci U S A. 1990 May;87(9):3445–3449. [PubMed]
Emini, EA; Nara, PL; Schleif, WA; Lewis, JA; Davide, JP; Lee, DR; Kessler, J; Conley, S; Matsushita, S; Putney, SD, et al. Antibody-mediated in vitro neutralization of human immunodeficiency virus type 1 abolishes infectivity for chimpanzees. J Virol. 1990 Aug;64(8):3674–3678. [PubMed]
Nara, PL; Garrity, RR; Goudsmit, J. Neutralization of HIV-1: a paradox of humoral proportions. FASEB J. 1991 Jul;5(10):2437–2455. [PubMed]
Berman, PW; Gregory, TJ; Riddle, L; Nakamura, GR; Champe, MA; Porter, JP; Wurm, FM; Hershberg, RD; Cobb, EK; Eichberg, JW. Protection of chimpanzees from infection by HIV-1 after vaccination with recombinant glycoprotein gp120 but not gp160. Nature. 1990 Jun 14;345(6276):622–625. [PubMed]
Putkonen, P; Thorstensson, R; Walther, L; Albert, J; Akerblom, L; Granquist, O; Wadell, G; Norrby, E; Biberfeld, G. Vaccine protection against HIV-2 infection in cynomolgus monkeys. AIDS Res Hum Retroviruses. 1991 Mar;7(3):271–277. [PubMed]
James, K; Bell, GT. Human monoclonal antibody production. Current status and future prospects. J Immunol Methods. 1987 Jun 26;100(1-2):5–40. [PubMed]
Huse, WD; Sastry, L; Iverson, SA; Kang, AS; Alting-Mees, M; Burton, DR; Benkovic, SJ; Lerner, RA. Generation of a large combinatorial library of the immunoglobulin repertoire in phage lambda. Science. 1989 Dec 8;246(4935):1275–1281. [PubMed]
Caton, AJ; Koprowski, H. Influenza virus hemagglutinin-specific antibodies isolated from a combinatorial expression library are closely related to the immune response of the donor. Proc Natl Acad Sci U S A. 1990 Aug;87(16):6450–6454. [PubMed]
Persson, MA; Caothien, RH; Burton, DR. Generation of diverse high-affinity human monoclonal antibodies by repertoire cloning. Proc Natl Acad Sci U S A. 1991 Mar 15;88(6):2432–2436. [PubMed]
Burton, DR. Human and mouse monoclonal antibodies by repertoire cloning. Trends Biotechnol. 1991 May;9(5):169–175. [PubMed]
Barbas, CF, 3rd; Kang, AS; Lerner, RA; Benkovic, SJ. Assembly of combinatorial antibody libraries on phage surfaces: the gene III site. Proc Natl Acad Sci U S A. 1991 Sep 15;88(18):7978–7982. [PubMed]
Kang, AS; Barbas, CF; Janda, KD; Benkovic, SJ; Lerner, RA. Linkage of recognition and replication functions by assembling combinatorial antibody Fab libraries along phage surfaces. Proc Natl Acad Sci U S A. 1991 May 15;88(10):4363–4366. [PubMed]
Chomczynski, P; Sacchi, N. Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal Biochem. 1987 Apr;162(1):156–159. [PubMed]
Parmley, SF; Smith, GP. Antibody-selectable filamentous fd phage vectors: affinity purification of target genes. Gene. 1988 Dec 20;73(2):305–318. [PubMed]
Rath, S; Stanley, CM; Steward, MW. An inhibition enzyme immunoassay for estimating relative antibody affinity and affinity heterogeneity. J Immunol Methods. 1988 Feb 10;106(2):245–249. [PubMed]
Goudsmit, J; Back, NK; Nara, PL. Genomic diversity and antigenic variation of HIV-1: links between pathogenesis, epidemiology and vaccine development. FASEB J. 1991 Jul;5(10):2427–2436. [PubMed]
Clackson, T; Hoogenboom, HR; Griffiths, AD; Winter, G. Making antibody fragments using phage display libraries. Nature. 1991 Aug 15;352(6336):624–628. [PubMed]
Winter, G; Milstein, C. Man-made antibodies. Nature. 1991 Jan 24;349(6307):293–299. [PubMed]