Purification and characterization of 2-aminoacetop...[J Biosci Bioeng. 2007]

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1: J Biosci Bioeng. 2007 Nov;104(5):416-9. Links

Purification and characterization of 2-aminoacetophenone reductase of newly isolated Burkholderia sp. YT.

Yamada-Onodera K, Takase Y, Tani Y.

Graduate School of Biological Sciences, Nara Institute of Science and Technology, 8916-5 Takayama, Ikoma 630-0192, Japan. onoderak@bs.naist.jp <onoderak@bs.naist.jp>

We found that a newly isolated Burkholderia sp. produced (R)-2-amino-1-phenylethanol from 2-aminoacetophenone, showing the high stereospecificity. NADPH-dependent 2-aminoacetophenone reductase purified to homogeneity was a dimer with a molecular mass of 65,000. The purified enzyme did not reduce acetophenone and 1-phenyl-1-propanone. The purified enzyme converted 2-aminoacetophenone to only (R)-2-amino-1-phenylethanol.

PMID: 18086443 [PubMed - indexed for MEDLINE]

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Purification and characterization of 2-aminoacetophenone reductase of newly isolated Burkh...Purification and characterization of 2-aminoacetophenone reductase of newly isolated Burkholderia sp. YT.

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Purification and characterization of 2-aminoacetophenone reductase of newly isolated Burkholderia sp. YT.

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