Bibliographic Citation
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Title | Radiation response of human lung cancer cells with inherent and acquired resistance to cisplatin |
Creator/Author | Twentyman, P.R. ; Wright, K.A. ; Rhodes, T. (MRC Clinical Oncology and Radiotherapeutics Unit, Cambridge (England)) |
Publication Date | 1991 Feb 01 |
OSTI Identifier | OSTI ID: 6057457 |
Other Number(s) | ISSN0360-3016; CODEN: IOBPD |
Resource Type | Journal Article |
Resource Relation | International Journal of Radiation Oncology, Biology and Physics ; Vol/Issue: 20:2 |
Subject | 560120 -- Radiation Effects on Biochemicals, Cells, & Tissue Culture; ANTINEOPLASTIC DRUGS-- RADIOSENSITIVITY EFFECTS;LUNGS-- CARCINOMAS;TUMOR CELLS-- RADIOSENSITIVITY; DOSE-RESPONSE RELATIONSHIPS;GLUTATHIONE;MAN |
Related Subject | ANIMAL CELLS;ANIMALS;BODY;DISEASES;DRUGS;MAMMALS;NEOPLASMS;ORGANIC COMPOUNDS;ORGANS;PEPTIDES;POLYPEPTIDES;PRIMATES;PROTEINS;RADIOPROTECTIVE SUBSTANCES;RESPIRATORY SYSTEM;VERTEBRATES |
Description/Abstract | We have derived sublines of three human lung cancer cell lines with acquired resistance to cisplatin.^The cisplatin resistant sublines of NCI-H69 (small cell), COR-L23 (large cell), and MOR (adenocarcinoma) show 5.3 fold, 3.1 fold, and 3.8 fold resistance, respectively, determined in a 6-day MTT assay.^Although the parent lines show a wide range of glutathione content per cell, the sublines each show similar values to their corresponding parent line.^Radiation response curves have been obtained using a soft agar clonogenic assay.^Values obtained for the parent lines (95% CL in parentheses) were: NCI-H69: Do = 0.99 Gy (0.87-1.16), n = 2.9 (1.6-5.2), GSH = 14 ng/10(4) cells; COR-L23: Do = 1.23 Gy (1.05-1.49), n = 1.3 (0.7-2.2), GSH = 47 ng/10(4) cells; MOR: Do = 1.66 Gy (1.48-1.88), n = 3.0 (1.9-4.8), GSH = 86 ng/10(4) cells.^The cisplatin resistant variants of NCI-H69 and COR-L23 showed 31% and 63% increases, respectively, in Do compared to their parent lines, whereas no change in radiation response was seen in MOR.^In this panel of lines, therefore, although there is a correlation between glutathione content and radiosensitivity of the parent cell lines, acquired resistance to cisplatin is not accompanied by increased glutathione content.^However, two of the three cisplatin resistant lines do show a significantly reduced radiosensitivity. |
Country of Publication | United States |
Language | English |
Format | Pages: 217-220 |
System Entry Date | 2001 May 13 |
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