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Title Radiation response of human lung cancer cells with inherent and acquired resistance to cisplatin
Creator/Author Twentyman, P.R. ; Wright, K.A. ; Rhodes, T. (MRC Clinical Oncology and Radiotherapeutics Unit, Cambridge (England))
Publication Date1991 Feb 01
OSTI IdentifierOSTI ID: 6057457
Other Number(s)ISSN0360-3016; CODEN: IOBPD
Resource TypeJournal Article
Resource RelationInternational Journal of Radiation Oncology, Biology and Physics ; Vol/Issue: 20:2
Subject560120 -- Radiation Effects on Biochemicals, Cells, & Tissue Culture; ANTINEOPLASTIC DRUGS-- RADIOSENSITIVITY EFFECTS;LUNGS-- CARCINOMAS;TUMOR CELLS-- RADIOSENSITIVITY; DOSE-RESPONSE RELATIONSHIPS;GLUTATHIONE;MAN
Related SubjectANIMAL CELLS;ANIMALS;BODY;DISEASES;DRUGS;MAMMALS;NEOPLASMS;ORGANIC COMPOUNDS;ORGANS;PEPTIDES;POLYPEPTIDES;PRIMATES;PROTEINS;RADIOPROTECTIVE SUBSTANCES;RESPIRATORY SYSTEM;VERTEBRATES
Description/Abstract We have derived sublines of three human lung cancer cell lines with acquired resistance to cisplatin.^The cisplatin resistant sublines of NCI-H69 (small cell), COR-L23 (large cell), and MOR (adenocarcinoma) show 5.3 fold, 3.1 fold, and 3.8 fold resistance, respectively, determined in a 6-day MTT assay.^Although the parent lines show a wide range of glutathione content per cell, the sublines each show similar values to their corresponding parent line.^Radiation response curves have been obtained using a soft agar clonogenic assay.^Values obtained for the parent lines (95% CL in parentheses) were: NCI-H69: Do = 0.99 Gy (0.87-1.16), n = 2.9 (1.6-5.2), GSH = 14 ng/10(4) cells; COR-L23: Do = 1.23 Gy (1.05-1.49), n = 1.3 (0.7-2.2), GSH = 47 ng/10(4) cells; MOR: Do = 1.66 Gy (1.48-1.88), n = 3.0 (1.9-4.8), GSH = 86 ng/10(4) cells.^The cisplatin resistant variants of NCI-H69 and COR-L23 showed 31% and 63% increases, respectively, in Do compared to their parent lines, whereas no change in radiation response was seen in MOR.^In this panel of lines, therefore, although there is a correlation between glutathione content and radiosensitivity of the parent cell lines, acquired resistance to cisplatin is not accompanied by increased glutathione content.^However, two of the three cisplatin resistant lines do show a significantly reduced radiosensitivity.
Country of PublicationUnited States
LanguageEnglish
FormatPages: 217-220
System Entry Date2001 May 13

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