Energy Citations Database

Bibliographic Citation

 
Document
For copies of Journal Articles, please contact the Publisher or your local public or university library and refer to the information in the Resource Relation field.
For copies of other documents, please see the Availability, Publisher, Research Organization, Resource Relation and/or Author (affiliation information) fields and/or Document Availability.
Title Transcriptional control of ADH genes in the xylose-fermenting yeast Pichia stipitis
Creator/Author Cho, J.Y. ; Jeffries, T.W. [Forest Service, Madison, WI (United States). Forest Products Lab.]|[Univ. of Wisconsin, Madison, WI (United States). Dept. of Bacteriology]
Publication Date1999 Jun 01
OSTI IdentifierOSTI ID: 354324
Other Number(s)AEMIDF; ISSN 0099-2240
Resource TypeJournal Article
Resource RelationApplied and Environmental Microbiology ; VOL. 65 ; ISSUE: 6 ; PBD: Jun 1999
Sponsoring OrgNational Renewable Energy Lab., Golden, CO (United States);Department of Agriculture, Washington, DC (United States)
Subject09 BIOMASS FUELS ; XYLOSE; FERMENTATION; YEASTS; TRANSCRIPTION; GENE REGULATION; ALCOHOL DEHYDROGENASE
Description/Abstract The authors studied the expression of the genes encoding group 1 alcohol dehydrogenases (PsADH1 and PsADH2) in the xylose-fermenting yeast Pichia stipitis CBS 6054. The cells expressed PsADH1 approximately 10 times higher under oxygen-limited conditions than under fully aerobic conditions when cultivated on xylose. Transcripts of PsADH2 were not detectable under either aeration condition. The authors used a PsADH1::lacZ fusion to monitor PsADH1 expression and found that expression increased as oxygen decreased. The level of PsADH1 transcript was expressed about 10-fold in cells grown in the presence of heme under oxygen-limited conditions. Concomitantly with the induction of PsADH1, PsCYC1 expression was regressed. These results indicate that oxygen availability regulates PsADH1 expression and that regulation may be mediated by heme. The regulation of PsADH2 expression was also examined in other genetic backgrounds. Disruption of PsADH1 dramatically increased PsADH2 expression on nonfermentable carbon sources under fully aerobic conditions, indicating that the expression of PsADH2 is subject to feedback regulation under these conditions.
Country of PublicationUnited States
LanguageEnglish
Formatpp. 2363-2368 ; PL:
System Entry Date2001 May 04

Top