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An organized view of the transcripome



Library:5159 (dbEST ID)

HT0447

Library Description

Organism:Homo sapiens
Developmental stage:Adult
Organ:head_neck
Vector:puc18
Vector type:Plasmid
Cloning site 1:SmaI
Cloning site 2:SmaI

A mini-library was made by cloning products derived from ORESTES PCR (U.S. Letters Patent application No. 196,716 - Ludwig Institute for Cancer Research) profiles into the pUC 18 vector. Reverse transcription of tissue mRNA and cDNA amplification were performed under low stringency conditions.

Source: Simpson A.J.G., Ludwig Institute for Cancer Research

Gene Content Analysis

24 ESTs from this library were grouped into 24 UniGene entries (putative genes) [UniGene build #217, 04-Dec-2008]. EST counts for each entry may be used to calculate an approximate expression level in transcripts per million (TPM).

ESTs TPM   UniGene Entry
1 41667 Hs.716979 Transcribed locus, strongly similar to NP_536848.1 ATP synthase F0 subunit 6 [Homo sapiens].
1 41667 Hs.715779 Transcribed locus.
1 41667 Hs.713441 MHC class I antigen HLA-A.
1 41667 Hs.711490 Nuclear antigen H731-like protein.
1 41667 Hs.69293 Beta-hexosaminidase beta chain.
1 41667 Hs.692284 Transcribed locus.
1 41667 Hs.675928 Transcribed locus.
1 41667 Hs.655019 CDNA FLJ38710 fis, clone KIDNE2002980.
1 41667 Hs.654400 Full length insert cDNA YQ19A04.
1 41667 Hs.638144 Transcribed locus.
1 41667 Hs.622504 Transcribed locus.
1 41667 Hs.533913 CDNA FLJ60799 complete cds.
1 41667 Hs.524506 ANKRD33 mRNA for ankyrin repeat domain 33.
1 41667 Hs.523789 Trophoblast MHC class II suppressor mRNA, complete sequence.
1 41667 Hs.504856 CDNA FLJ43113 fis, clone CTONG2028208.
1 41667 Hs.480876 Anaphase promoting complex subunit 10, mRNA (cDNA clone MGC:12234 IMAGE:3929766).
1 41667 Hs.444451 Mixed lineage kinase.
1 41667 Hs.439060 Full length insert cDNA clone ZE03C06.
1 41667 Hs.407580 P0071 protein.
1 41667 Hs.325846 Zinc finger CCCH-type containing 14, mRNA (cDNA clone IMAGE:4904207).
1 41667 Hs.309641 CDNA FLJ77817 complete cds, highly similar to Homo sapiens ring finger protein 11 (RNF11), mRNA.
1 41667 Hs.29189 Homo sapiens, clone IMAGE:4427831, mRNA.
1 41667 Hs.257008 Phospholipase D family, member 3, mRNA (cDNA clone MGC:35422 IMAGE:5189261).
1 41667 Hs.24135 Vezatin, adherens junctions transmembrane protein, mRNA (cDNA clone IMAGE:4823231).

Source: NCBI UniGene

EST Sequences

67 EST sequences from this library have been submitted to the public sequence database as of 04-Dec-2008.

Source: NCBI GenBank

Click the button to save sequences to a file (FASTA format)

Keywords

  • thyroid
  • head and neck tumor
  • adult

Source: NCBI Clone Registry

References

Shotgun sequencing of the human transcriptome with ORF expressed sequence tags. Dias Neto E, et al., Proc Natl Acad Sci U S A 97, 3491-3496 (2000).



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