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Library:3131 (dbEST ID)
CT0277
Library Description
| Organism: | Homo sapiens |
| Developmental stage: | Adult |
| Organ: | colon |
| Vector: | puc18 |
| Vector type: | Plasmid |
| Cloning site 1: | SmaI |
| Cloning site 2: | SmaI |
A mini-library was made by cloning products derived from ORESTES PCR (U.S. Letters Patent application No. 196,716 - Ludwig Institute for Cancer Research) profiles into the pUC 18 vector. Reverse transcription of tissue mRNA and cDNA amplification were performed under low stringency conditions.
Source: Simpson A.J.G., Ludwig Institute for Cancer Research
Gene Content Analysis
28 ESTs from this library were grouped into 26 UniGene entries (putative genes) [UniGene build #217, 04-Dec-2008]. EST counts for each entry may be used to calculate an approximate expression level in transcripts per million (TPM).
| ESTs | TPM | UniGene Entry | ||
|---|---|---|---|---|
| 2 | 71429 |  |
Hs.683477 | Transcribed locus. |
| 2 | 71429 | |
Hs.520814 | Tensin 3. |
| 1 | 35714 | |
Hs.716416 | Pig8 (PIG8). |
| 1 | 35714 | |
Hs.714722 | Dimethylarginine dimethylaminohydrolase 1 (DDAH1), transcript variant 2, mRNA. |
| 1 | 35714 | |
Hs.703502 | Transcribed locus, strongly similar to XP_001144282.1 PREDICTED: phosphorylase kinase, gamma 2 (testis) [Pan troglodytes]. |
| 1 | 35714 | |
Hs.671070 | Transcribed locus. |
| 1 | 35714 | |
Hs.666716 | Transcribed locus. |
| 1 | 35714 | |
Hs.657350 | INE1 mRNA. |
| 1 | 35714 | |
Hs.655066 | Zinc finger, BED-type containing 5, mRNA (cDNA clone IMAGE:4686017). |
| 1 | 35714 | |
Hs.644639 | Eukaryotic translation elongation factor 1 alpha 1, mRNA (cDNA clone MGC:9345 IMAGE:3461588). |
| 1 | 35714 | |
Hs.527021 | R-ras3. |
| 1 | 35714 | |
Hs.518827 | Cyclin I, mRNA (cDNA clone MGC:3795 IMAGE:2957878). |
| 1 | 35714 | |
Hs.518750 | CDNA FLJ58028 complete cds, highly similar to Homo sapiens OCIA domain containing 1 (OCIAD1), mRNA. |
| 1 | 35714 | |
Hs.496710 | CDNA FLJ58155 complete cds, highly similar to Cohesin subunit SA-2. |
| 1 | 35714 | |
Hs.469116 | Na+/H+ exchanger NHE-1 isoform [human, heart, mRNA, 4516 nt]. |
| 1 | 35714 | |
Hs.438336 | Chromosome 2 open reading frame 30 (C2orf30), transcript variant 2, mRNA. |
| 1 | 35714 | |
Hs.433422 | Nuclear protein p30. |
| 1 | 35714 | |
Hs.356721 | Transcribed locus, strongly similar to NP_058944.1 heterogeneous nuclear ribonucleoprotein A1 [Rattus norvegicus]. |
| 1 | 35714 | |
Hs.355126 | Transmembrane channel-like 4, mRNA (cDNA clone IMAGE:5215240). |
| 1 | 35714 | |
Hs.343522 | Cardiac plasma membrane Ca2+-ATPase variant 4b hPMCA4b. |
| 1 | 35714 | |
Hs.274873 | CDNA FLJ59524 complete cds, highly similar to Cysteinyl-tRNA synthetase (EC 6.1.1.16). |
| 1 | 35714 | |
Hs.26704 | Cytoplasmic FMR1 interacting protein 1 (CYFIP1), transcript variant 1, mRNA. |
| 1 | 35714 | |
Hs.252549 | Cathepsin Z precursor (CTSZ). |
| 1 | 35714 | |
Hs.2161 | C5a anaphylatoxin receptor. |
| 1 | 35714 | |
Hs.164226 | (clone lambda-TS-33) thrombospondin (THBS) mRNA, 5' end. |
| 1 | 35714 | |
Hs.163173 | CDNA FLJ78660 complete cds, highly similar to Homo sapiens interferon induced with helicase C domain 1 (IFIH1), mRNA. |
Source: NCBI UniGene
EST Sequences
66 EST sequences from this library have been submitted to the public sequence database as of 04-Dec-2008.
Source: NCBI GenBank
Click the button to save sequences to a file (FASTA format)
Keywords
- intestine
- colorectal tumor
- adult
Source: NCBI Clone Registry
References
Shotgun sequencing of the human transcriptome with ORF expressed sequence tags. Dias Neto E, et al., Proc Natl Acad Sci U S A 97, 3491-3496 (2000).