Degradation of Pseudoviral DNA after Infection of Mouse Cells with Polyoma Pseudovirions

Proc Natl Acad Sci U S A. 1974 October; 71(10): 3834–3838.

PMCID: PMC434278

Degradation of Pseudoviral DNA after Infection of Mouse Cells with Polyoma Pseudovirions^*

S. V. S. Kashmiri^† and H. Vasken Aposhian^‡

¹Department of Cell Biology and Pharmacology, University of Maryland School of Medicine, Baltimore, Md. 21201

^† Present address: The Wistar Institute, Philadelphia, Pa. 19104.

^‡ To whom reprint requests should be addressed.

^* This is publication no. 3 in a series on polyoma pseudovirions. Paper 2 is ref. 6.

This article has been cited by other articles in PMC.

Abstract

When mouse cells are infected with [³H]-thymidine-labeled polyoma pseudovirions, as much as 11% of the input pseudoviral radioactivity is found in the recipient cell DNA. However, the radioactivity found in the recipient cell DNA virtually disappears when nonradioactive thymidine and deoxycytidine, or D-arabinosyl cytosine, are added to the medium. The radioactivity found in the recipient cell DNA appears to be the result of the breakdown of pseudoviral DNA to precursors of DNA and the utilization of these precursors for the synthesis of cell DNA.

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Selected References

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