Construction and characterization of an isogenic urease-negative mutant of Helicobacter mustelae.

Infect Immun. 1995 September; 63(9): 3718–3721.

PMCID: PMC173517

J V Solnick, C Josenhans, S Suerbaum, L S Tompkins, and A Labigne

Division of Infectious Diseases, University of California, Davis, School of Medicine, USA.

This article has been cited by other articles in PMC.

Abstract

Helicobacter mustelae infects the ferret stomach and provides an opportunity to study pathogenic determinants of a Helicobacter species in its natural host. We constructed an isogenic urease-negative mutant of H. mustelae which produced no detectable urease and showed a reduced acid tolerance. This mutant provides an opportunity to further evaluate the role of urease in the pathogenesis of Helicobacter infection.

Full Text

The Full Text of this article is available as a PDF (229K).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

Clyne, M; Labigne, A; Drumm, B. Helicobacter pylori requires an acidic environment to survive in the presence of urea. Infect Immun. 1995 May;63(5):1669–1673. [PubMed]
Creno, RJ; Wenk, RE; Bohlig, P. Automated micromeasurement of urea using urease and the Berthelot reaction. Am J Clin Pathol. 1970 Dec;54(6):828–832. [PubMed]
Dixon, MF. Helicobacter pylori and peptic ulceration: histopathological aspects. J Gastroenterol Hepatol. 1991 6(2):125–130.Mar–Apr; [PubMed]
Dunn, BE; Sung, CC; Taylor, NS; Fox, JG. Purification and characterization of Helicobacter mustelae urease. Infect Immun. 1991 Sep;59(9):3343–3345. [PubMed]
Eaton, KA; Krakowka, S. Effect of gastric pH on urease-dependent colonization of gnotobiotic piglets by Helicobacter pylori. Infect Immun. 1994 Sep;62(9):3604–3607. [PubMed]
Ferrero, RL; Cussac, V; Courcoux, P; Labigne, A. Construction of isogenic urease-negative mutants of Helicobacter pylori by allelic exchange. J Bacteriol. 1992 Jul;174(13):4212–4217. [PubMed]
Ferrero, RL; Labigne, A. Cloning, expression and sequencing of Helicobacter felis urease genes. Mol Microbiol. 1993 Jul;9(2):323–333. [PubMed]
Fox, JG; Correa, P; Taylor, NS; Lee, A; Otto, G; Murphy, JC; Rose, R. Helicobacter mustelae-associated gastritis in ferrets. An animal model of Helicobacter pylori gastritis in humans. Gastroenterology. 1990 Aug;99(2):352–361. [PubMed]
Fox, JG; Otto, G; Taylor, NS; Rosenblad, W; Murphy, JC. Helicobacter mustelae-induced gastritis and elevated gastric pH in the ferret (Mustela putorius furo). Infect Immun. 1991 Jun;59(6):1875–1880. [PubMed]
Heffron, F; McCarthy, BJ; Ohtsubo, H; Ohtsubo, E. DNA sequence analysis of the transposon Tn3: three genes and three sites involved in transposition of Tn3. Cell. 1979 Dec;18(4):1153–1163. [PubMed]
Hu, LT; Mobley, HL. Purification and N-terminal analysis of urease from Helicobacter pylori. Infect Immun. 1990 Apr;58(4):992–998. [PubMed]
Labigne, A; Courcoux, P; Tompkins, L. Cloning of Campylobacter jejuni genes required for leucine biosynthesis, and construction of leu-negative mutant of C. jejuni by shuttle transposon mutagenesis. Res Microbiol. 1992 Jan;143(1):15–26. [PubMed]
Labigne, A; Cussac, V; Courcoux, P. Shuttle cloning and nucleotide sequences of Helicobacter pylori genes responsible for urease activity. J Bacteriol. 1991 Mar;173(6):1920–1931. [PubMed]
Marshall, BJ; Armstrong, JA; McGechie, DB; Glancy, RJ. Attempt to fulfil Koch's postulates for pyloric Campylobacter. Med J Aust. 1985 Apr 15;142(8):436–439. [PubMed]
Morris, A; Nicholson, G. Ingestion of Campylobacter pyloridis causes gastritis and raised fasting gastric pH. Am J Gastroenterol. 1987 Mar;82(3):192–199. [PubMed]
Morris, AJ; Ali, MR; Nicholson, GI; Perez-Perez, GI; Blaser, MJ. Long-term follow-up of voluntary ingestion of Helicobacter pylori. Ann Intern Med. 1991 Apr 15;114(8):662–663. [PubMed]
Nomura, A; Stemmermann, GN; Chyou, PH; Kato, I; Perez-Perez, GI; Blaser, MJ. Helicobacter pylori infection and gastric carcinoma among Japanese Americans in Hawaii. N Engl J Med. 1991 Oct 17;325(16):1132–1136. [PubMed]
Parsonnet, J; Friedman, GD; Vandersteen, DP; Chang, Y; Vogelman, JH; Orentreich, N; Sibley, RK. Helicobacter pylori infection and the risk of gastric carcinoma. N Engl J Med. 1991 Oct 17;325(16):1127–1131. [PubMed]
Sanger, F; Nicklen, S; Coulson, AR. DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463–5467. [PubMed]
Segal, ED; Shon, J; Tompkins, LS. Characterization of Helicobacter pylori urease mutants. Infect Immun. 1992 May;60(5):1883–1889. [PubMed]
Sly, LI; Bronsdon, MA; Bowman, JP; Holmes, A; Stackebrandt, E. The phylogenetic position of Helicobacter nemestrinae. Int J Syst Bacteriol. 1993 Apr;43(2):386–387. [PubMed]
Solnick, JV; O'Rourke, J; Lee, A; Tompkins, LS. Molecular analysis of urease genes from a newly identified uncultured species of Helicobacter. Infect Immun. 1994 May;62(5):1631–1638. [PubMed]
Solnick, JV; O'Rourke, J; Lee, A; Paster, BJ; Dewhirst, FE; Tompkins, LS. An uncultured gastric spiral organism is a newly identified Helicobacter in humans. J Infect Dis. 1993 Aug;168(2):379–385. [PubMed]
Suerbaum, S; Josenhans, C; Labigne, A. Cloning and genetic characterization of the Helicobacter pylori and Helicobacter mustelae flaB flagellin genes and construction of H. pylori flaA- and flaB-negative mutants by electroporation-mediated allelic exchange. J Bacteriol. 1993 Jun;175(11):3278–3288. [PubMed]
Tsuda, M; Karita, M; Morshed, MG; Okita, K; Nakazawa, T. A urease-negative mutant of Helicobacter pylori constructed by allelic exchange mutagenesis lacks the ability to colonize the nude mouse stomach. Infect Immun. 1994 Aug;62(8):3586–3589. [PubMed]
Turbett, GR; Høj, PB; Horne, R; Mee, BJ. Purification and characterization of the urease enzymes of Helicobacter species from humans and animals. Infect Immun. 1992 Dec;60(12):5259–5266. [PubMed]
Winship, PR. An improved method for directly sequencing PCR amplified material using dimethyl sulphoxide. Nucleic Acids Res. 1989 Feb 11;17(3):1266. [PubMed]