RFE Fish Species Identification Isoelectric Focusing Tools

U.S. Food & Drug Administration
Seafood Products Research Center
Center for Food Safety & Applied Nutrition
Regulatory Fish Encyclopedia:
IEF Gel Lane Banding Pattern Peak Report Data

Peak Report Data from Isoelectric Focusing for a specimen of:
Starry Flounder ---- Go to Display Gel Lane Navigator.

FDA Seafood List Scientific Name: Platichthys stellatus
FDA Seafood List Common Name: Starry Flounder
FDA Seafood List Market Name: Flounder
Unique IEF Gel Code: 19950809gel02
Unique Web Code: sfh01Srr
Tissue Aliquot Code: platstel H95-005 #02a Gel Analysis Conditions


Gel Image	Fish Image	Calibrated Gel

DISPLAY GEL LANE NAVIGATOR
RETURN TO TOP OF THIS DOCUMENT
Lane a pI Markers	Lane b Specimen	Lane c pI Markers	Lane d Co-Migrated	Lane e Specimen	Lane f pI Markers	Go to Gel Schematic

**DISPLAY GEL LANE LETTER:** Lane a
**DIGITAL REPORT GEL LANE:** 2
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 2
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.55	97	11.5	0.0291415	5	0.239381	8.5	5.6	4.55	96.8	4.7	0.0287072
2	5.2	196	23.1	0.0274274	4	0.189304	6.7	4.4	5.2	195.8	4.1	0.0260927
3	5.85	291	34.4	0.0224729	6	0.20328	7.2	4.8	5.85	290.9	5.7	0.0200289
4	6.55	412	48.6	0.0261842	6	0.289296	10.3	6.8	6.55	412.2	6.5	0.0251506
5	7.35	510	60.2	0.0105587	6	0.103433	3.7	2.4	7.35	509	5.8	0.0100254
6	8.15	633	74.7	0.0417849	6	0.450681	16	10.6	8.15	632.2	6.3	0.0402363
7	8.45	695	82.1	0.0545175	6	0.550663	19.5	12.9	8.45	695	5.7	0.0540563
8	8.65	749	88.4	0.0428706	4	0.33936	12	8	8.65	748.5	4.5	0.0428587
9	9.3	818	96.6	0.0392678	7	0.452328	16.1	10.6	9.3	817.1	7.1	0.0360322

PeakCounter: 9

**DISPLAY GEL LANE LETTER:** Lane b
**DIGITAL REPORT GEL LANE:** 3
**LANE TYPE:** Specimen
**ACTUAL GEL LANE:** 3
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.101	31	3.7	0.150063	9	2.39421	11.3	8.2	4.101	31.8	9.4	0.144154
2	4.633	108	12.8	0.0695818	6	0.73717	3.5	2.5	4.633	108.4	6.1	0.0682405
3	4.937	152	18	0.0102139	4	0.0763119	0.4	0.3	4.937	150.9	4.2	0.0101756
4	5.287	203	24	0.0128339	5	0.0994814	0.5	0.3	5.287	202.8	4.5	0.0124156
5	5.388	218	25.8	0.234205	3	1.3872	6.6	4.7	5.388	217.9	3.3	0.235728
6	5.481	232	27.5	0.107853	4	0.653244	3.1	2.2	5.481	232.8	3.5	0.104182
7	5.676	261	30.9	0.357582	4	2.19604	10.4	7.5	5.676	261.2	3.7	0.332732
8	5.79	278	32.9	0.0685391	14	1.58827	7.5	5.4	5.79	275.9	14.1	0.0636008
9	5.896	295	34.9	0.0230397	4	0.0893586	0.4	0.3	5.896	295	3.9	0.0127837
10	5.959	306	36.2	0.0103053	1	0.00525495	0	0	5.959	306.3	0.6	0.00488163
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	6.09	329	38.9	0.0325586	5	0.307121	1.5	1	6.09	331.5	4.9	0.0354887
12	6.147	339	40.1	0.234029	2	0.867006	4.1	3	6.147	338.5	2	0.245887
13	6.187	346	40.9	0.327171	3	1.68694	8	5.7	6.187	346.6	2.9	0.33239
14	6.301	366	43.3	0.098285	4	0.193176	0.9	0.7	6.301	363.9	3.7	0.0293317
15	6.347	374	44.3	0.0926094	15	2.27862	10.8	7.8	6.347	373.4	15.2	0.0846859
16	6.473	396	46.9	0.19215	2	0.670658	3.2	2.3	6.473	395.6	2.2	0.172068
17	6.843	446	52.8	0.0837846	3	0.421136	2	1.4	6.843	445.4	2.8	0.0853242
18	7.474	528	62.5	0.0914418	18	2.90121	13.7	9.9	7.474	532.2	17.9	0.0914762
19	7.644	554	65.6	0.154559	11	2.57539	12.2	8.8	7.644	556	11	0.131701

PeakCounter: 19

**DISPLAY GEL LANE LETTER:** Lane c
**DIGITAL REPORT GEL LANE:** 4
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 4
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.55	95	11.3	0.030507	5	0.258836	8.2	5.9	4.55	95	4.9	0.0296339
2	5.2	184	21.8	0.0288643	5	0.226632	7.2	5.2	5.2	184.6	4.6	0.0276436
3	5.85	283	33.6	0.0224399	6	0.217835	6.9	5	5.85	283.1	5.7	0.0216792
4	6.55	407	48.3	0.0384422	7	0.428946	13.6	9.8	6.55	406.4	6.7	0.0359584
5	7.35	508	60.3	0.0148934	5	0.130478	4.1	3	7.35	506.8	5.1	0.0144594
6	8.15	630	74.7	0.0432477	6	0.470926	15	10.8	8.15	629.2	6.4	0.0417243
7	8.45	691	82	0.0489159	6	0.558294	17.7	12.8	8.45	691.5	6.4	0.0488468
8	8.65	746	88.5	0.0451786	5	0.366359	11.6	8.4	8.65	745.4	4.6	0.0447066
9	9.3	814	96.6	0.0427368	7	0.487572	15.5	11.2	9.3	813.5	6.6	0.0417267

PeakCounter: 9

**DISPLAY GEL LANE LETTER:** Lane d
**DIGITAL REPORT GEL LANE:** 5
**LANE TYPE:** Co-Migrated
**ACTUAL GEL LANE:** 5
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.128	36	4.3	0.0658796	11	1.17981	8.8	6.8	4.128	36.5	10.8	0.0616188
2	4.55	97	11.5	0.0150429	4	0.0997274	0.7	0.6	4.55	96.9	4.4	0.0127144
3	4.619	107	12.7	0.0248913	6	0.247423	1.8	1.4	4.619	106.8	5.5	0.025342
4	4.944	154	18.3	0.00971603	4	0.0878483	0.7	0.5	4.944	152.6	4	0.0124257
5	5.2	191	22.7	0.0145601	5	0.117089	0.9	0.7	5.2	190.6	4.6	0.0143014
6	5.411	221	26.2	0.113011	4	0.785244	5.8	4.5	5.411	221.3	3.9	0.114828
7	5.517	236	28	0.041728	4	0.309713	2.3	1.8	5.517	236.3	4.1	0.042496
8	5.693	261	31	0.203294	5	1.73588	12.9	10	5.693	260.2	4.9	0.197963
9	5.77	272	32.3	0.0797036	8	1.04226	7.8	6	5.77	272.7	7.6	0.0775764
10	6.129	333	39.5	0.118488	5	0.976916	7.3	5.6	6.129	332.3	5	0.111033
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	6.174	341	40.5	0.22803	3	1.32226	9.8	7.6	6.174	341.1	3.4	0.222412
12	6.297	363	43.1	0.06051	12	1.00769	7.5	5.8	6.297	362	11.8	0.0482289
13	6.348	372	44.1	0.058412	11	0.721266	5.4	4.2	6.348	376.4	11.5	0.0354989
14	6.483	396	47	0.0916857	3	0.369736	2.7	2.1	6.483	396	2.5	0.0831798
15	6.55	408	48.4	0.00536828	3	0.0192339	0.1	0.1	6.55	409	2.9	0.00377135
16	6.872	447	53	0.0431997	6	0.415805	3.1	2.4	6.872	445.3	5.7	0.0409197
17	7.35	505	59.9	0.0180366	5	0.17052	1.3	1	7.35	503.5	5.3	0.0181051
18	7.497	528	62.6	0.0288505	12	0.60366	4.5	3.5	7.497	531.4	12.1	0.0280846
19	7.67	555	65.8	0.0639218	13	1.37425	10.2	7.9	7.67	555.6	12.7	0.0608137
20	8.45	693	82.2	0.0326123	6	0.317756	2.4	1.8	8.45	692.2	5.5	0.0325246
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
21	8.65	744	88.3	0.0275829	5	0.208108	1.5	1.2	8.65	743.4	4.5	0.0258861
22	9.3	813	96.4	0.0217122	10	0.334172	2.5	1.9	9.3	812.7	9.6	0.0196532

PeakCounter: 22

**DISPLAY GEL LANE LETTER:** Lane e
**DIGITAL REPORT GEL LANE:** 6
**LANE TYPE:** Specimen
**ACTUAL GEL LANE:** 6
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.099	31	3.7	0.125438	9	1.99847	12.6	8.8	4.099	32.2	9.3	0.121336
2	4.609	104	12.3	0.0497853	7	0.595469	3.8	2.6	4.609	104.4	7	0.0478275
3	4.924	149	17.7	0.00927942	4	0.0576571	0.4	0.3	4.924	149.2	4.1	0.00801784
4	5.312	205	24.3	0.00980686	4	0.0637752	0.4	0.3	5.312	203.9	4	0.00896595
5	5.413	220	26.1	0.186824	4	1.25313	7.9	5.5	5.413	219.6	3.7	0.190266
6	5.528	237	28.1	0.0974297	4	0.651472	4.1	2.9	5.528	236.9	3.9	0.0951383
7	5.704	263	31.2	0.354891	4	2.21791	14	9.8	5.704	262.9	3.7	0.341236
8	5.791	276	32.7	0.0977608	8	1.19857	7.6	5.3	5.791	275.6	7.5	0.0899951
9	5.886	291	34.5	0.0258479	4	0.1775	1.1	0.8	5.886	290	4.3	0.0235326
10	5.936	300	35.6	0.00874901	3	0.0388867	0.2	0.2	5.936	301.6	3.5	0.00634162
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	6.038	318	37.7	0.0388715	3	0.163257	1	0.7	6.038	317.8	3.5	0.0266839
12	6.122	333	39.5	0.327118	7	2.88712	18.3	12.8	6.122	330.9	6.6	0.247805
13	6.235	353	41.9	0.0792889	5	0.660956	4.2	2.9	6.235	353.1	4.8	0.0774351
14	6.291	363	43.1	0.0672179	5	0.609184	3.9	2.7	6.291	363	5.3	0.0649907
15	6.353	374	44.4	0.0484901	6	0.48851	3.1	2.2	6.353	372.7	5.5	0.0497616
16	6.46	393	46.6	0.156738	3	0.874021	5.5	3.9	6.46	392.5	3.2	0.153456
17	6.868	448	53.1	0.0720749	3	0.381689	2.4	1.7	6.868	448.2	3	0.0710915
18	7.466	525	62.3	0.0164962	5	0.136861	0.9	0.6	7.466	525.4	4.5	0.0169998
19	7.673	557	66.1	0.0873142	9	1.35306	8.6	6	7.673	555.7	9.1	0.083971

PeakCounter: 19

**DISPLAY GEL LANE LETTER:** Lane f
**DIGITAL REPORT GEL LANE:** 7
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 7
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.55	90	10.7	0.0304467	6	0.30232	8.8	6.5	4.55	89.6	5.7	0.0300181
2	5.2	182	21.7	0.0274222	7	0.309512	9	6.7	5.2	182.3	6.7	0.0261516
3	5.85	282	33.6	0.0169048	8	0.238097	6.9	5.1	5.85	282.3	8.4	0.0159071
4	6.55	406	48.4	0.0289754	8	0.401267	11.6	8.6	6.55	404.7	8.2	0.027769
5	7.35	505	60.2	0.0238122	6	0.231529	6.7	5	7.35	505.2	5.6	0.0233621
6	8.15	628	74.9	0.0453648	7	0.517072	15	11.1	8.15	628.1	6.7	0.0435844
7	8.45	691	82.4	0.0646351	6	0.635637	18.4	13.7	8.45	690.5	5.7	0.0634639
8	8.65	745	88.8	0.0523407	5	0.435829	12.6	9.4	8.65	744.5	4.8	0.050737
9	9.3	809	96.4	0.0371779	6	0.380309	11	8.2	9.3	808.3	6	0.0359593

PeakCounter: 9

Gel Banding Patterns Display in HTML using GIF stacking technique
(Uses Migration Position Normalized Data from above text peak reports)
Return to Lane Navigator Go to Gel Schematic Key

a b c d e f

MK SP MK CO SP MK

pI 4.55 label for gel schematic

pI 5.20 label for gel schematic

pI 5.8 label for gel schematic

pI 6.55 label for gel schematic

pI 7.35 label for gel schematic

pI 8.15 label for gel schematic

pI 8.45 label for gel schematic

pI 8.65 label for gel schematic

pI 9.30 label for gel schematic

pI 4.55 label for gel schematic

pI 5.20 label for gel schematic

pI 5.8 label for gel schematic

pI 6.55 label for gel schematic

pI 7.35 label for gel schematic

pI 8.15 label for gel schematic

pI 8.45 label for gel schematic

pI 8.65 label for gel schematic

pI 9.30 label for gel schematic

Key to Gel Schematic:

MK=pI Marker Lanes, pI bands are in red
SP=Specimen Lanes, Specimen bands are in blue
CO=Co-migrated Lanes of pI Markers and Specimen (pI bands are in red, Specimen bands are in blue)
Note: In co-migrated lanes, some pI Markers and Specimen bands may overlap in experiment. Red pI
band is reported when overlapped peak position is equal to nominal pI Marker position. Blue Specimen
band is reported when overlapped peak position deviates slightly from nominal pI Marker position.
Weak (<2% rel. intensity), Medium (2-6% rel. intensity), and Strong (>6% rel. intensity) band intensities indicated by gel schematic band thickness.
Return to Top of Gel Schematic

PeakCount: 87

HTML Forms TextArea Normalized Peak-Position Peak-Area for Copy-Paste events
4.550 8.500 5.200 6.700 5.850 7.200 6.550 10.300 7.350 3.700 8.150 16.000 8.450 19.500 8.650 12.000 9.300 16.100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane a DIGITAL REPORT GEL LANE: 2 LANE TYPE: pI Markers ACTUAL GEL LANE: 2 PeakCount: 9 Return to Lane Navigator
4.101 11.300 4.633 3.500 4.937 0.400 5.287 0.500 5.388 6.600 5.481 3.100 5.676 10.400 5.790 7.500 5.896 0.400 5.959 0 6.090 1.500 6.147 4.100 6.187 8.000 6.301 0.900 6.347 10.800 6.473 3.200 6.843 2.000 7.474 13.700 7.644 12.200 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane b DIGITAL REPORT GEL LANE: 3 LANE TYPE: Specimen ACTUAL GEL LANE: 3 PeakCount: 19 Return to Lane Navigator
4.550 8.200 5.200 7.200 5.850 6.900 6.550 13.600 7.350 4.100 8.150 15.000 8.450 17.700 8.650 11.600 9.300 15.500 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane c DIGITAL REPORT GEL LANE: 4 LANE TYPE: pI Markers ACTUAL GEL LANE: 4 PeakCount: 9 Return to Lane Navigator
4.128 8.800 4.550 0.700 4.619 1.800 4.944 0.700 5.200 0.900 5.411 5.800 5.517 2.300 5.693 12.900 5.770 7.800 6.129 7.300 6.174 9.800 6.297 7.500 6.348 5.400 6.483 2.700 6.550 0.100 6.872 3.100 7.350 1.300 7.497 4.500 7.670 10.200 8.450 2.400 8.650 1.500 9.300 2.500 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane d DIGITAL REPORT GEL LANE: 5 LANE TYPE: Co-Migrated ACTUAL GEL LANE: 5 PeakCount: 22 Return to Lane Navigator
4.099 12.600 4.609 3.800 4.924 0.400 5.312 0.400 5.413 7.900 5.528 4.100 5.704 14.000 5.791 7.600 5.886 1.100 5.936 0.200 6.038 1.000 6.122 18.300 6.235 4.200 6.291 3.900 6.353 3.100 6.460 5.500 6.868 2.400 7.466 0.900 7.673 8.600 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane e DIGITAL REPORT GEL LANE: 6 LANE TYPE: Specimen ACTUAL GEL LANE: 6 PeakCount: 19 Return to Lane Navigator
4.550 8.800 5.200 9.000 5.850 6.900 6.550 11.600 7.350 6.700 8.150 15.000 8.450 18.400 8.650 12.600 9.300 11.000 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane f DIGITAL REPORT GEL LANE: 7 LANE TYPE: pI Markers ACTUAL GEL LANE: 7 PeakCount: 9 Return to Lane Navigator

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EXPERIMENTAL CONDITIONS: Gels were scanned at 600 d.p.i. in Adobe Photoshop 3.02 using a Sharp JX-325 Scanner with Film Scanner Attachment. Gels were analyzed for Densitometry, Intregration, and Migration Position Normalization in EXCEL 5.0 Format. *.xls files were imported into Paradox 7.0 to save as *.db files.

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Delphi 4.0 Generation of this Isoelectric Focusing (IEF) RFE PAGE 5 Tools in HTML
This HTML is automatically generated from a series of Paradox 7.0 database filesof IEF peak reports is controlled with a main RFEIEF Paradox 7.0 database withDelphi 3.0 front end (see Delphi 2.0 form) and back end for facile addition and modification of the Content of these RFE PAGE 5 HTML tools.
Contact Brad Tenge at btenge@ora.fda.gov for details.

RFE Contacts
A summary list of RFE contact personnel is available at http://www.cfsan.fda.gov/~frf/rfecon.html.

For isoelectric focusing laboratory experiments for fish species identification, you may contact

Brad Tenge at E-mail: btenge@ora.fda.gov
Ngoc-Lan Nguyen at E-mail: nnguyen@ora.fda.gov
Jim Barnett at E-mail: jbarnett@ora.fda.gov
Pat Rogers at E-mail: pxr@cfsan.fda.gov

For isoelectric focusing gel analysis and pattern recognition applications, you may contact

Brad Tenge at E-mail: btenge@ora.fda.gov
Ngoc-Lan Nguyen at E-mail: nnguyen@ora.fda.gov
Fred Fry at E-mail frf@vm.cfsan.fda.gov

For CFSAN web consultation and posting, you may contact

Fred Fry E-mail: frf@vm.cfsan.fda.gov

Thank you for using the Regulatory Fish Encyclopedia (RFE)!

RFE Species Browse | RFE Tutorial | Seafoods | CFSAN

Last updated on 2003-FEB-27 by bjt/frf/nln

Peak Report Data from Isoelectric Focusing for a specimen of: Starry Flounder ---- Go to Display Gel Lane Navigator.

Peak Report Data from Isoelectric Focusing for a specimen of:
Starry Flounder ---- Go to Display Gel Lane Navigator.