TITLE Microbial Volatile Organic Compound Production by Indoor Air Microorganisms AUTHOR(S) Joan C. Rivers, Joachim D. Pliel, Russell W. Wiener PERFORMING ORGANIZATION NAME AND ADDRESS ManTech Environmental Technology Research Triangle Park, NC 27709 SPONSORING AGENCY NAME AND ADDRESS U. S. EPA AREAL/MRDD/APMB MD-77 Research Triangle Park, NC 27711 ABSTRACT Indoor air quality is a significant environmental and occupational health issue. The production of volatile organic compounds (VOCs) by metabolically active microorganisms is important to indoor air quality because many microbial VOCs have offensive odors and can potentially cause adverse health effects. The identification of microbial VOCs and factors which influence their production is important to understanding the contribution of microbially-produced VOCs to total indoor air quality. In this study, several unidentified fungal and bacterial strains were isolated from indoor air environments and grown on a variety of substrates. VOC characterizations were conducted on microbial headspace air by gas chromatography/mass spectroscopy analysis. Several VOCs were detected that may adversely affect indoor air quality. Some of these compounds include methyl mercaptan, dimethyldisulfide, dimethyltrisulfide, trimethylamine and indole. Factors which influence microbial VOC production were identified.