Klein A, Gordon P, Ramcharitar S, Mercure L, Malkin A, Wainberg M; International Conference on AIDS.
Int Conf AIDS. 1989 Jun 4-9; 5: 604 (abstract no. W.C.P.74).
Clinical Biochemistry Dept., Sunnybrook Medical Centre, University of Toronto, Toronto, Ontario, Canada
High content of unsaturated fatty acids (FAS) in membrane lipids lead to an increase in cell membrane fluidity and can explain cell fusion and death through the loss of membrane integrity. In the present work after H-9 and MT-4 lymphocyte cell lines were infected with HIV, a quantitative and qualitative analysis of their FAS were made. These cell lines were chosen because MT-4 is killed after HIV infection and H-9 is not. Infected and non-infected cells were extracted with methanol:chloroform solutions. The lipids were transmethylated to produce FA methyl esters and analyzed by gas chromatography. HIV infection was characterized by: 1) a significant rise (180-250%) (p less than 0.05) in the desaturation of essential FAS--represented by linolenic (18:3) and arachidonic (20:4) acids--in both cell lines, 2) a significant elevation (p less than 0.05) in the concentration of the saturated FA stearate (18:0) (180%) and palmitate (16:0) (187%) only in the MT-4 cell line, and 3) a significant rise (p less than 0.05) in the desaturation of stearate to oleate (18:0 to 18:1) (171%) only in the MT-4 cell line. The results demonstrate that infection by HIV is characterized by elevated desaturation of essential FAS, whereas killing of cells shows increase in both formation of non-essential FAS and desaturation of stearate. The mechanism by which these changes take place is not at all clear.
Publication Types:
Keywords:
- AIDS Vaccines
- Acquired Immunodeficiency Syndrome
- Cell Line
- Cell Membrane
- Fatty Acids, Unsaturated
- HIV
- HIV Seropositivity
- HIV-1
- Humans
- Lipids
- Lymphocytes
- Membrane Fluidity
- Membrane Lipids
- Oleic Acid
- Palmitates
- Stearates
Other ID:
UI: 102179083
From Meeting Abstracts