Klein A, Malkin A, Bruser B, Li G, Volsky DJ, Mizrachi Y; International Conference on AIDS.
Int Conf AIDS. 1990 Jun 20-23; 6: 141 (abstract no. F.A.262).
Dept. of Biochemistry, Sunnybrook Med. Ctr., University of Toronto, Toronto, Canada
OBJECTIVES: High content of unsaturated fatty acids (FA) accompanied by low content of saturated FAs lead to increase in cell membrane fluidity and can explain cell fusion and death through the loss of membrane integrity. It has been demonstrated that neural cells are susceptible to HIV infection. The aim of the present work was to test whether HIV infection leads to changes in the content of FAs in membrane of neuroblastoma cells. METHODS: Two neuroblastoma cell lines were used, one (SKNMC), susceptible to HIV infection and the other (2L1) not susceptible. They were grown in DMEM supplemented with 15% FCS. Cells were harvested and resuspended at 20x10(6) and infected with 100x concentrated virus diluted 1/10 for each time point. Cells were incubated for 0, 15, 30, and 60 min., washed and extracted according to the Folch method. The lipids were hydrolysed and transmethylated to produce FA methyl esters and analysed by gas chromatography. The contents of the following FAs were measured: Palmitic (16:0), 2. Stearic (18:0), 3. Oleic (18:1), 4. Linoleic (18:2), 5. Arachidonic (20:4), and 6. Docosahexaenoic (22:6). RESULTS: The comparison of FA contents in cell membrane of the two cell lines showed no difference among the corresponding FAs at the intervals of 0 - 30 min. However, 60 min after infection, the content of arachidonate increased 170% (p less than 0.01) and the content of stearate decreased to 77% (p less than 0.05) in SKNMC cell. No similar changes were observed with 2L1 cells. CONCLUSION: The present results suggest that HIV infection leads to changes in the saturated/unsaturated FAs ratio which may increase the fluidity of the membrane and cause alteration in the neural cell membrane function.
Publication Types:
Keywords:
- AIDS Vaccines
- Cell Line
- Cell Membrane
- Fatty Acids, Unsaturated
- HIV
- HIV-1
- Humans
- Lipids
- Membrane Fluidity
- Neurons
Other ID:
UI: 102184161
From Meeting Abstracts