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Title T-lymphocyte induction of human monocyte angiotensin converting enzyme (ACE) is not dependent upon T-lymphocyte proliferation
Creator/Author Vuk-Pavlovic, Z. ; Rohrbach, M.S.
Publication Date1986 Mar 05
OSTI IdentifierOSTI ID: 7135053
Report Number(s)CONF-8604222-
Other Number(s)CODEN: FEPRA
Resource TypeJournal Article
Resource RelationFed. Proc., Fed. Am. Soc. Exp. Biol. ; Vol/Issue: 45:4; 70. annual meeting of the Federation of American Society for Experimental Biology; 13 Apr 1986; St. Louis, MO, USA
Research OrgMayo Clinic, Rochester, MI
Subject550201 -- Biochemistry-- Tracer Techniques; LYMPHOCYTES-- CELL PROLIFERATION;LYMPHOCYTES-- ENZYME INDUCTION; CANDIDA;CELL CULTURES;DOSE-RESPONSE RELATIONSHIPS;ENZYME ACTIVITY;MONOCYTES;TRACER TECHNIQUES;TRITIUM COMPOUNDS
Related SubjectANIMAL CELLS;BIOLOGICAL MATERIALS;BLOOD;BLOOD CELLS;BODY FLUIDS;CONNECTIVE TISSUE CELLS;FUNGI;GENE REGULATION;ISOTOPE APPLICATIONS;LABELLED COMPOUNDS;LEUKOCYTES;MATERIALS;MICROORGANISMS;PLANTS;SOMATIC CELLS;YEASTS
Description/Abstract Human peripheral blood monocytes cultured in serum free media for seven days show a basal activity of the ectoenzyme ACE which is augmented 2-3 times by the presence of autologous peripheral blood T-lymphocytes.^Since these two cell types are also involved in autologous mixed lymphocyte reaction if serum is present, the authors compared the ability of T-cells to stimulate ACE activity in the presence or absence of proliferation (measured by /sup 3/H-thymidine incorporation).^By the seventh day, cultures with 5% AB/sup +/ serum showed significant increase in proliferation but no increase in ACE activity compared to the serum free cultures.^Even higher proliferation rate achieved by co-culturing T-lymphocytes with allogeneic monocytes did not increase ACE production; on the contrary, ACE activity remained at the basal level.^Monocyte-T-cell co-cultures stimulated with increasing concentrations of ConA or PHA showed dose dependent increases in proliferation but parallel decreases in ACE activity.^Addition of soluble antigen (Candida albicans) also enhanced proliferation but not ACE synthesis.^They conclude that T-lymphocyte induction of monocyte ACE is a result of cooperation between autologous cells which is not dependent upon T-cell proliferation.
Country of PublicationUnited States
LanguageEnglish
FormatPages: 1116
System Entry Date2001 May 13

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